Searchable abstracts of presentations at key conferences in endocrinology
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23rd Joint Meeting of the British Endocrine Societies with the European Federation of Endocrine Societies

Poster Presentations

Neuroendocrinology and behaviour

ea0007p145 | Neuroendocrinology and behaviour | BES2004

The insulin tolerance test (ITT) in clinical practice: the experience of a busy pituitary unit

Liew A , Agha A , Finucane F , Thornton E , Rogers B , Tormey W , Thompson C

The insulin tolerance test (ITT) is the gold standard for assessing growth hormone (GH) and cortisol production in pituitary disease, However it is potentially hazardous, requires medical supervision and adequate hypoglycemia may not be achieved. We retrospectively examined the records of 185 consecutive patients aged 14-76 years with pituitary disease who underwent the ITT over 18 months. Testing was performed in the endocrine day ward by an experienced endocrine nurse. Absol...

ea0007p146 | Neuroendocrinology and behaviour | BES2004

Cardiovascular risk burden in adult patients with hypopituitarism

Kyaw|#Tun T , Agha A , Muigai N , Al-Sahli W , Rogers B , Tormey W , Thompson C

Hypopituitarism is associated with premature vascular mortality. Growth hormone (GH) deficiency, over-replacement with glucocorticoid, cranial irradiation and traditional vascular risk factors are possible contributing factors to the excess mortality.We studied 200 consecutive adult hypopituitary age (mean standard deviation) 51.7 plus/minus 15.3 years, who attended our pituitary clinic between July and December 2002. Hypertension was defined as blood pr...

ea0007p147 | Neuroendocrinology and behaviour | BES2004

Incorporating social preferences in the measurement of QoL outcomes in growth hormone deficiency (GHD) in adults

Kind P , Koltowska-Haggstrom M

Background:.Quality of Life Assessment of Growth Hormone Deficiency in Adults (QoL-AGHDA) was designed for use in patients with GHD, a condition characterized amongst other things, by impairment in QoL. For use in cost-utility analysis outcomes should be measured on a scale of social preferences that assigns values of 1 and 0 to full health and dead. Condition-specific instruments may generate summary index scores but typically lack the legitimacy for use as measures of QoL ou...

ea0007p148 | Neuroendocrinology and behaviour | BES2004

Neuroendocrine abnormalities in the acute phase of traumatic brain injury

Rogers B , Agha A , Mylotte D , Tormey W , Phillips J , Thompson C

Pituitary hormone abnormalities have been reported in up to 50% of survivors of traumatic brain injury (TBI), tested several months or longer after the event. No information is available on the frequency of hypopituitarism in the immediate post-TBI period.We studied 50 consecutive patients who were admitted to the neurosurgical unit with severe or moderate TBI [initial Glasgow Coma Scale (GCS) score 3-13]. Testing was performed at a median of 12 days pos...

ea0007p149 | Neuroendocrinology and behaviour | BES2004

Investigating the potential role of melanocortin receptor 5 in the rodent adrenal gland

Kosti O , Renshaw D , Harrold J , Cone R , Hinson J

Two members of the melanocortin (MC) receptor family are abundantly expressed in the adrenal cortex, namely MC2R and MC5R. MC2R binds only ACTH and is known as the ACTH receptor, the role of MC5R however is still under investigation. It is known that alpha-MSH stimulates aldosterone secretion by the rat adrenal cortex, by an MC2R-independent mechanism, although the receptor involved has not been identified. The experiments were designed to investigate the role of MC5R in the a...

ea0007p150 | Neuroendocrinology and behaviour | BES2004

Quality of life deficit in hypopituitary adults with growth hormone deficiency (GHD): comparison of age-sex matched patient data and a UK population survey

Koltowska-Haggstrom M , Monson J , Hennessy S , Mattsson A , Kind P

Background: Although the negative impact of GHD in adults on quality of life (QoL) is well recognized and it has become routinely measured in clinical practice, there is still limited information about the extent of the differential between adults with GHD and their age/sex-matched peers within the general population.Objectives: The study was designed to test the discriminatory power of QoL-AGHDA by determining normative reference values in the general p...

ea0007p151 | Neuroendocrinology and behaviour | BES2004

Downward resetting of the osmotic threshold for thirst in patients with syndrome of inappropriate antidiuretic hormone

Smith D , Moore K , Tormey W , Baylis P , Thompson C

The syndrome of inappropriate antidiuretic hormone (SIADH) is characterised by euvolamic hyponatraemia. Patients with SIADH continue to drink normal amounts of fluid, despite plasma osmolalities well below the physiological osmotic threshold for the onset of thirst. The regulation of thirst has not been previously studied in SIADH. We studied the characteristics of osmotically-stimulated thirst and vasopressin (AVP) secretion, and the non-osmotic suppression of thirst and AVP ...

ea0007p152 | Neuroendocrinology and behaviour | BES2004

Nephrogenic diabetes insipidus in the mouse does not deplete vasopressin neurons or activate oxytocin neurons

Morris J , Epton M , Jenkins D

Hereditary nephrogenic diabetes (di/di) in mice results from a failure of vasopressin (VP)action caused by overactive renal cAMP phosphodiesterase activity. The adult animals pass large amounts of dilute (100 mOsm per kg) urine and have hyperosmotic plasma (40 mOsm per kg greater than wild-type). Their osmotic status is therefore similar to that in Brattleboro rats in which both VP and oxytocin (OT) neurons are hyperactive and the neural lobe is depleted of both OT and VP. The...

ea0007p153 | Neuroendocrinology and behaviour | BES2004

Comparison of anterior pituitary annexin 1 expression in male/female and old/young Long Evans and Brattleboro rats

Davies E , Christian H , Buckingham J , Morris J

It has been noted that there are differences in the hypothalamic-pituitary-adrenal (HPA) axis between the sexes, as well as changes in the axis with age. The differences in the HPA axis between the gender and age groups are thought to be due to differences in the efficiency of glucocorticoid (GC) negative feedback. The pituitary, a principle site of GC negative feedback, is the focus of this investigation. Annexin-1 (ANXA1) is a member of the annexin family of phospholipid- an...

ea0007p154 | Neuroendocrinology and behaviour | BES2004

Prevalence of hypopituitarism in survivors of traumatic brain injury

Agha A , Rogers B , Tormey W , Phillips J , Thompson C

Recent evidence suggests a high prevalence of neuroendocrine dysfunction in patients following traumatic brain injury (TBI), but dynamic anterior pituitary assessment were performed in relatively small number of patients and posterior pituitary function remains poorly investigated.We studied 102 consecutive patients (84 males) who had survived severe or moderate TBI [initial Glasgow Coma Scale (GCS) score 3-13] at a mean (+/- standard deviation) of 19 +/...

ea0007p155 | Neuroendocrinology and behaviour | BES2004

The effect of oestrogen replacement therapy (ORT) on growth hormone (GH) dose; KIMS database perspective

Mah P , Ross R , Jonsson P , Feldt-Rasmussen U , Koltowska-Haggstrom M , Webster J

Oral but not transdermal ORT reduces serum IGF-1 levels in postmenopausal women. The effect of ORT type on GH dose and sensitivity is unclear. We present a retrospective analysis of GH-deficient women on ORT in the KIMS database.AIM: To determine the type of oestrogen prescribed and its effect on GH dose.METHOD: Patients were divided into groups taking oral oestradiol valerate, conjugated oestrogen, ethinyloestradiol and transderma...

ea0007p156 | Neuroendocrinology and behaviour | BES2004

Metastatic glucagonoma transforming to an insulinoma

Byard A , Rangan S , Dixon A , Kos K , Edavalath M , Buch H , Singh B

Neuroendocrine tumours are uncommon and often present difficult therapeutic challenges. They may be composed of different cell types and are often multihormonal. We present the case of a 75 year-old man diagnosed to have a glucagonoma in 1995. He was deemed unfit for curative surgery and was managed medically including insulin therapy for control of hyperglycaemia. Over the next 7 years the patient became progressive more symptomatic due to increase in tumour size and hepatic ...

ea0007p157 | Neuroendocrinology and behaviour | BES2004

Investigation of the hypothalamo-pituitary-adrenal axis in patients with irritable bowel syndrome

Baskol M , Gokce C , Yucesoy M , Kelestimur F

Irritable bowel syndrome (IBS) which is very common is characterized by chronic intermittent symptoms, including recurrent abdominal pain, altered frequency of defecation, stool urgency and excess flatus. Widespread symptoms associated with IBS are abnormal defecation and abdominal pain, both of which can be exacerbated by psychogenic stress. IBS is one of the stress-related bodily disorders and it has been suggested that patients with IBS may exhibit disturbances in the hypot...

ea0007p158 | Neuroendocrinology and behaviour | BES2004

DNA microarray analysis of NECA action in a pituitary folliculostellate cell line

Rees D , Scanlon M , Ham J

Folliculostellate (FS) cells are non-hormone producing cells that represent up to 10% of the anterior pituitary gland and may mediate the response of the hypothalamic pituitary adrenal axis to inflammation. Adenosine, released during stress and inflammation, acts at A2b receptors to stimulate growth and secretion of IL-6 from a mouse FS cell line, TtT/GF. We have now extended these studies and investigated how NECA (universal adenosine receptor agonist; 10 micromolar) stimulat...

ea0007p159 | Neuroendocrinology and behaviour | BES2004

Imaging of the pituitary is indicated in marginal hyperprolactinaemia (<1000 milliunits per litre) to detect microadenoma

Shore H , Karavitaki N , Turner H , Wass J

Background: In clinical practice there is considerable confusion as to whether pituitary imaging is indicated in patients presenting with a marginally elevated serum prolactin level (<1000 milliunits per litre) and no other discernible cause of hyperprolactinaemia.Objective: The aim of this study was to investigate the frequency of radiological abnormalities of the pituitary in patients presenting with marginal hyperprolactinaemia (<1000 milliunits...

ea0007p160 | Neuroendocrinology and behaviour | BES2004

Effects of melatonin on catecholaminergic neurotransmitters in brain areas regulating food intake in the male rat

Canpolat S , Sandal S , Kutlu S , Aydin M , Yilmaz B , Kelestimur H

Brain catecholaminergic neurotransmitters are involved in the regulation of food intake. Increased dopamin and noradrenaline release in the lateral hypothalamic area (LHA) results in inhibition of food intake whereas stimulative effects have been reported in the medial nucleus. Elevated noradrenaline levels have been shown in the hypothalamus and nucleus accumbens (NAC) of hyperphagic-hypermetabolic rats. Effects of melatonin on food intake in various species have been controv...

ea0007p161 | Neuroendocrinology and behaviour | BES2004

The acute octreotide suppression test is useful in predicting long-term responses to depot somatostatin analogue injections in patients with active acromegaly

Botusan I , Karavitaki N , Radian S , Turner H , Wass J

Background: Long-acting depot somatostatin analogues injections are useful drugs in the medical treatment of acromegaly. No previous study has assessed the value of acute octreotide suppression test in predicting responses to depot injections.Objective: The aim of this study was to investigate the value of the acute octreotide suppression test (100 micrograms subcutaneously, GH measurements hourly for 8 hours) in predicting the long-term response to long...

ea0007p162 | Neuroendocrinology and behaviour | BES2004

Effects of melatonin on the regulation of leptin production in rat anterior pituitary cells

Kus I , Sarsilmaz M , Colakoglu N , Kukner A , Ozen O , Yilmaz B , Kelestimur H

Leptin, which is secreted mainly by adipocytes and some other tissues including pituitary, informs the brain about the mass of adipose tissue and has an important role in energy homeostasis. We have previously shown that melatonin decreases circulating leptin levels. Whether melatonin has an effect on leptin synthesis in pituitary is currently unknown. Therefore, the aim of this study was to immunohistochemically examine the effects of pinealectomy and administration of melato...

ea0007p163 | Neuroendocrinology and behaviour | BES2004

Primary therapy with somatostatin analogues in acromegaly does not restore orderly GH secretion

Parkinson C , Darzy K , Peacey S , Thorner M , Veldhuis J , Trainer P , Shalet S

Tumoural GH secretion in acromegaly is characterised by increased non-pulsatile (basal) release, pulse amplitude and pulse frequency. We have studied the effect of primary therapy with Sandostatin LAR on GH pulsatility in 9 patients with acromegaly who achieved disease control (mean serum GH<2ug/L) on therapy (7 males; median (range) age 67 (43-75). Data obtained were compared with 16 healthy subjects (10 males, age 50 (30-75)). Spontaneous 24-h GH secretion (20 minute samp...

ea0007p164 | Neuroendocrinology and behaviour | BES2004

Down-regulation of neurotrophin receptor expression limits the regenerative potential of injured CNS neurons

Brown E , Berry M , Logan A

The application of exogenous neurotrophins (NT) has been widely proposed as a therapeutic strategy for the recovery of regeneration in axotomised CNS neurons. Results from this approach have, however, been disappointing since limited regenerative responses are elicited, we suggest due to limited neurotrophin receptor (NTR) expression. NT exert their trophic effects by signalling through the Trk/p75 receptors. Adequate receptor expression levels must, therefore, be central to t...

ea0007p165 | Neuroendocrinology and behaviour | BES2004

Functional interactions between anosmin-1, heparan sulphate and urokinase-type plasminogen activator and the pathogenesis of X-linked Kallmann's Syndrome

Hu Y , Gonzalez-Martinez D , Bouloux P

Anosmin-1, a protein defective in X-linked Kallmann's syndrome (X-KS), presents a structure comprising a cysteine rich N terminus (CR), a whey acid protein (WAP) domain followed by four fibronectin-like III domains (FnIII); and its function is heparan sulphate (HS) dependent. However, anosmin-1 and HS binding affinity is unknown. Although the WAP domain belongs to a protein family demonstrating serine protease inhibitory activity, the interacting serine protease remains to be ...

ea0007p166 | Neuroendocrinology and behaviour | BES2004

Are cortisol responses to psychological stress related to size at birth in humans?

Ward A , Moore V , Steptoe A , Robinson J , Phillips D

Prenatal manipulations in animal models result in lifelong alterations in the stress responsivity of the hypothalamic-pituitary-adrenal axis (HPAA). While several human studies have found raised 0900h cortisol concentrations in low birthweight individuals, twenty-four hour cortisol profiles do not vary according to birthweight (a marker of adverse antenatal exposures). One explanation for this dichotomy is that 0900h cortisol concentration measured in a novel clinic setting ma...

ea0007p167 | Neuroendocrinology and behaviour | BES2004

Addition of dopamine agonists to somatostatin analogue therapy improves biochemical control of acromegaly

Selverajah D , Webster J , Ross R , Newell-Price J

Background: The introduction of somatostatin analogues for the treatment of acromegaly has relegated dopamine agonists, once a mainstay of treatment, down the therapeutic ladder. Dopamine agonists are, however, added to somatostatin analogues to control active disease in some patients, in an attempt to achieve biochemical control. There are no reports, however, assessing this practise.Aim: To assess the effectiveness of adding dopamine agonist therapy to...

ea0007p168 | Neuroendocrinology and behaviour | BES2004

Variability in GH assays undermines the value of consensus criteria for the diagnosis of adult GH deficiency and acromegaly

Pokrajac-Simeunovic A , Wieringa G , Ellis A , Trainer P

There is increasing reliance on published consensus criteria for clinical decision-making in states of GH excess and deficiency. NICE eligibility criteria for GH treatment include a peak GH response <9 mU/L during an ITT. To determine the adequacy of GH assay performance for diagnosing GH deficiency (GHD), we have assessed the variability in 101 UKNEQAS reported results from a single sample with a value close to 9.0 mU/L. For all laboratories (n=101) median GH was 11.1 mU/L...

ea0007p169 | Neuroendocrinology and behaviour | BES2004

The role of the melanocortin 5 receptor in the hypothalamic regulation of appetite and pituitary function

Sajedi A , Abbott C , Small C , Haskell-Luevano C , Ghatei M , Bloom S

The melanocortin receptors (MCR), MC3R and MC4R, have established roles in feeding and pituitary function. MC5R mRNA expression has been identified at low levels in the hypothalamus. However, a role for this receptor in appetite and pituitary function remains to be elucidated. We investigated the effects of central administration of an MC5R agonist (3,3,3-triphenylpropionyl-His-D-Phe-Arg-Trp-NH2) on food intake and plasma pituitary hormone levels. We did not observe...