Searchable abstracts of presentations at key conferences in endocrinology
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10th European Congress of Endocrinology

ea0016p484 | Obesity | ECE2008

The influence of low calorie-high dietary fibres diet on the change of parameters of the metabolic syndrome

Polovina Snezana , Milenkovic Koviljka , Novkovic Mile

Metabolic syndrome is risk factor for cardiovascular event. According recommendation of NCEP ATP III, the major component of metabolic syndrome is waist circumference up to 94 cm in male and 80 cm in female, and two of following disturbances: fasting glucose up to 5.6 mmol/l, HDL below 0.9 in male and 1.3 in female, triglycerides up to 1.7 mmol/l and BP up to 135/80 mmHg.We have analyzed the influence of low calorie diet (1200–1500 kcal/day) with in...

ea0016p485 | Obesity | ECE2008

Estradiol reverts the impairment of the natriuretic peptide system in a menopause-associated obesity model

Belo Najara , Reis Adelina , Sairam Malur

The follicle-stimulating hormone receptor knockout (FORKO) female mouse provides a useful model to examine the role that lack of estrogen plays in the development of obesity and hypertension in postmenopausal women. Estrogen increases circulating levels of atrial natriuretic peptide (ANP), a hormone with renal and cardiovascular effects. It has been shown that atrial natriuretic peptide (ANP) is a potent stimulator of fat cell lipolysis in addition of its well established effe...

ea0016p486 | Obesity | ECE2008

The effect of various dyslipidemia management guidelines on the assessment of LDL-C goal attainment in population-based studies

Farahani Pendar

Drug therapies are effective for improving lipid profiles. However, a large proportion of patients and specifically high-risk patients appear unable to achieve the recommended goals for LDL-C level. Furthermore, the proportions of patients, who lack the targeted LDL-C goal, are inconsistent among different studies.Objectives: To explore if application of different guidelines for the management of dyslipidemia can influence the proportion of LDL-C target ...

ea0016p487 | Obesity | ECE2008

Bariatric surgery for patients whit morbid obesity: a cohort study

Antolin Sandra Herranz , Pelayo Miriam Perez , Losada Tomas Gonzalez , de Dios Luis Munoz , Tudanca Sergio Fuentes , Garcia Dolores del Olmo , Echarri Jaime Vazquez

Objectives: Analyze the evolution of body mass index (BMI) and percentage of excess weight loss (PEWL) after bariatric surgery; compare adjustable gastric banding (LABG) vs laparoscopic gastric bypass (LGB).Methods: Cohort study. Inclusion criteria: all cases underwent laparoscopic bariatric surgery; n=89; 9 patients were excluded for incomplete follow up. Differences between the 2 groups were evaluated using the Student t-test; P&#6...

ea0016p488 | Obesity | ECE2008

Overweight and obesity in Iranian adolescents

Abdollahi Morteza , Abtahi Mitra , HoushiarRad Anahita

Introduction: The problem of increasing prevalence of overweight and obesity as a consequence of new life styles is worrying the scientists and health officers in less developed countries. In Iran that is experiencing an accelerated nutrition transition overweight has turned into a major public health problem.Objective: To determine overweight and obesity prevalence in Iranian adults.Material and methods: We have used data on 35 92...

ea0016p489 | Obesity | ECE2008

Obesity and it's hormonal profile in saudi population

Bahathiq Adil , Alharthy Afaf , Assery Ebtehaj , Nafady Hind

Objective: To evaluate and to study obesity in Saudi female population represente by Makkah community. This study aims to investigate obesity, by detecting leptin concentration and to take other parameters like body mass index (BMI) and waist circumference (WC) in assessing obesity.Methods: Two-hundred forty women (n=240) between the ages 18 and 65 participate in this study. Volunteers were divided into three groups. The first group was the normal...

ea0016p490 | Obesity | ECE2008

Adiponectin induces CXCL8 in primary human hepatocytes via the adiponectin receptor 1

Wanninger Josef , Neumeier Markus , Weigert Johanna , Weiss Thomas , Bollheimer Cornelius , Buettner Roland , Schaffler Andreas , Scholmerich Jurgen , Buechler Christa

Background: Adiponectin was suggested to exert hepatoprotective effects in animal models of tetrachlorcarbonate-, endotoxin- and alcohol-induced liver injury. Further, in patients with non-alcoholic fatty liver disease (NAFLD) low adiponectin levels were found to be associated with more severe steatosis and hepatic inflammation.Methods: To investigate the molecular basis of the hepatoprotective effects of adiponectin primary human hepatocytes were incuba...

ea0016p491 | Obesity | ECE2008

Iranian overweight children and adolescents: who are seeking weight loss treatment?

Maddah Mohsen

Objective: This study described overweight/obese children and adolescents seeking weight loss treatment regarding their age, gender, severity of obesity and maternal education in Rasht City, northern Iran.Design: A descriptive study on overweight children and adolescents setting: the main clinic of obesity management in Rasht, northern Iran.Subjects: Data on 1465 overweight/obese children and adolescents aged 2–18 years engage...

ea0016p492 | Obesity | ECE2008

Metabolic and hormonal patterns in men under the age 40 years with obesity and metabolic syndrome

Goncharov Nikolai , Katsya Gulinara , Chagina Nina

Objectives: To assess the androgens profile (total testosterone (TT),free testosterone (fT), SHBG, and DHEA-sulphate), cortisol, aldosterone, insulin, and leptin in patients with obesity (OB), metabolic syndrome (MS) and healthy men (HM).Subjects and methods: Group 1: 26 men with obesity, age 30±6 years. BMI 27–35 kg/m2. Group 2: 34 men with MS, age 31±6 yrs. BMI 30–36 kg/m2. Group 3: 20 healthy men, age 28±...

ea0016p493 | Obesity | ECE2008

Prevalence of overweight in comparison to wasting, among Iranian under 5 year-old children in urban and rural

Abtahi Mitra , Houshiarrad Anahita , Abdollahi Morteza , Mohammadpour Ahranjani Behnoush

Introduction and aim: Beside protein–energy malnutrition among children, high prevalence of overweight is also a public health problem and a major nutritional challenge in most developing countries. The aim of this research was to determine and compare the prevalence of wasting, overweight and obesity among Iranian urban and rural under 5 year -old children.Subjects and methods: This study was based on national comprehensive study on household food ...

ea0016p494 | Obesity | ECE2008

Gene expression profiling in skeletal muscle from diet-induced obesity susceptible rats

Buttner Roland , Schaffler Andreas , Buchler Christa , Wrede Christian , Scholmerich Jurgen , Bollheimer Cornelius

Background: The variablity of the individual susceptibility to diet-induced obesity and insulin resistance is well known, but so far insufficiently explained. As skeletal muscle metabolism and insulin sensitivity are important pathophysiologic features of obesity-asscociated metabolic perturbations, we tried to identify differences in muscular gene expression profiles between high fat fed rats prone to develop obesity (diet-induced obesity, DIO) and those which remained lean (...

ea0016p495 | Obesity | ECE2008

A pharmacologic sympathectomy prevents the development of obesity and insulin resistance in the high fat fed rat

Bollheimer Cornelius , Harle Peter , Schaffler Andreas , Buchler Christa , Wrede Christian , Straub Rainer , Scholmerich Jurgen , Buttner Roland

Background: Obese patients show an increased activity of the sympathetic nervous system, but the causality of the relationship is not known. Here, we examine whether a pharmacologic sympathectomy influences diet-induced obesity and insulin resistance in the high fat fed rat model.Methods: Wistar-Rats were fed ad libitum with a high fat diet (HF, 40 energy% of fat, based on lard) for 12 weeks. A pharmacologic sympathectomy was performed by a) weekl...

ea0016p496 | Obesity | ECE2008

Lipid-loaded hepatocytes release soluble factors that activate hepatic stellate cells: a new in vitro model to study fibrogenesis in NASH

Wobser Hella , Dorn Christoph , Weiss Thomas , Scholmerich Jurgen , Hellerbrand Claus

Background: Non-alcoholic steatohepatitis (NASH) can progress to hepatic fibrosis and end-stage liver disease. Hepatic stellate cells (HSC) are the central mediators of liver fibrosis. The molecular mechanisms linking hepatic steatosis to activation of HSC, thereby promoting inflammation and fibrosis, are mainly unknown. Here, we demonstrate a novel in vitro model to study the effect of hepatic lipid accumulation on HSC.Methods and results:...

ea0016p497 | Obesity | ECE2008

Insulin resistance, atherogenic dyslipidemia and other atherogenic factors in simple obesity and obesity with Acanthosis nigricans (an) in childhood

Ilyes Istvan , Felszeghy Eniko , Varadi Zita , Seres Ildiko , Paragh Gyorgy

The aim of the study was to investigate insulin resistance, dyslipidemia and atherogenic factors influencing HDL functions in symple obesity (SO) and obesity with acanthosis nigricans (OAN). Althogether 37 children with obesity, 17 girls and 20 boys, 19 with SO and 18 with OAN, were included into the study. Their age was 14.2±1.8 years and BMI was 35.9±6.1 kg/m2.Fasting glucose (FG), insulin (INS) were measured and HOMA-IR was calcul...

ea0016p498 | Obesity | ECE2008

Tribbles pseudokinases and adipose tissue biology

Ostertag Anke , Herzig Stephan

Obesity, insulin resistance and high cholesterol levels are hallmarks of the metabolic syndrome. The molecular players involved in the onset of the metabolic syndrome are mostly unknown. In this context the three pseudokinases of the tribbles family have emerged as potential candidates. Among them, TRB3 has been described as a negative regulator of Akt, a key player in insulin signal transduction. However, the biological functions of TRB family members remain largely unknown. ...

ea0016p499 | Obesity | ECE2008

Differences in the POMC methylation pattern in normal weight and obese individuals and regulation of MC-4R promoter activity by DNA methylation

Kuehnen Peter , Frank van Landeghem , Annette Grueters , Heiko Krude

The highly conserved Leptin-Melanocortin signaling pathway plays a central role in the regulation of body weight and energy expenditure. Mutations in POMC and MC-4R lead to dosage dependent monogenetic obesity. However, several evidences suggest an epigenetic impact on the regulation of body weight. Moreover it has been shown recently that variants of the FTO gene, which affects ss DNA methylation in the arcuate nucleus, correlate with increased body weight in children and adu...

ea0016p500 | Obesity | ECE2008

Retinol-binding protein 4 (RBP-4) levels do not change after oral glucose tolerance test in obese and overweight individuals, but correlate with some indices of insulin resistance

Lewandowski Krzysztof C , Basinska-Lewandowska Magdalena , Bienkiewicz Malgorzata , Randeva Harpal S , Lewinski Andrzej

Background: Secretory products from adipocytes may contribute to increased insulin resistance (IR). Retinol-binding protein-4 (RBP-4) may increase IR in mice, with elevated levels in insulin resistant mice and humans with obesity and type 2 diabetes. Mechanisms regulating RBP-4 synthesis remain not fully understood. It is not clear whether short-term hyperglycaemia alters RBP-4 levels in humans. In order to investigate this, we measured serum RBP-4 concentrations during 75 gra...

ea0016p501 | Obesity | ECE2008

Overweight adolescents, a group at risk for metabolic syndrome (Tehran Adolescent Obesity Study)

Moayeri Heshmat , Bidad Katayoon , Rabbani Ali , Keihani doust Zarrin

Background: Metabolic syndrome not only is a serious problem for adults, but is also afflicting an increasing number of children and adolescents. This syndrome is a risk factor for type 2 diabetes mellitus and cardiovascular diseases. The aim of this study was to estimate the prevalence of metabolic syndrome in a sample of Iranian adolescents.Methods: A total of 554 overweight adolescents (aged 11–17 years) participated in a community-based cross se...

ea0016p502 | Obesity | ECE2008

The obesity, physical activity status and dietary pattern in 10–12 years old girls of a mountainous region in north of Iran

Salarkia Nahid , Reineh Homeira Nasiri

Background: It has been shown that one of major causes of obesity in young people could be explained by physical inactivity and fat intake.Purpose: This study was carried out to investigate the prevalence of obesity, dietary pattern and physical activity status in Tonekabon girls, a mountainous city in north of Iran.Methods: In a cross-sectional study 311 girls aged 10–12 years old in Tonekabon were studied. Weight, height, wa...

ea0016p503 | Obesity | ECE2008

Expression of the different components of the endocannabinoid system (ECs) in morbid obesity

Vicennati Valentina , Pagotto Uberto , Cervino Cristina , Amenta Enrico , Cariani Stefano , Pasquali Renato

Visceral fat (VAT) may represent the more important site of endocannabinoid dysregulation in obesity. No data are available on the expression of the ECs in morbid obesity. Eleven morbidly obese women, who underwent bariatric surgery, were enrolled and paired samples of VAT and subcutaneous fat tissue (SAT) were obtained from each patient. Real-Time PCR for the gene expression levels of cannabinoid type 1 (CB1) receptor, N-acyl-phosphatidil-ethanolamine (NAPE), and fatty...

ea0016p504 | Obesity | ECE2008

Plasma adrenomedullin positively correlates to body mass index, and significantly decreases after bariatric surgery

Clodi Martin , Vila Greisa , Riedl Michaela , Handisurya Ammon , Maier Christina , Morgenthaler Nils G , Luger Anton

Adrenomedullin is a vasoactive peptide originally discovered in extracts of human pheochromocytoma. It is highly expressed in plasma and adipose tissue of obese subjects and considered as a member of the adipokine family. Adrenomedullin inhibits adipogenesis under the transcriptional control of insulin. We determined plasma adrenomedullin concentrations in a cohort of 360 healthy subjects and found a very significant positive correlation to body mass index (BMI). In parallel, ...

ea0016p505 | Obesity | ECE2008

Plasma osteopontin correlates with insulin resistance in obese subjects, but increases following bariatric surgery parallel to other markers of bone turnover

Riedl Michaela , Vila Greisa , Maier Christina , Handisurya Ammon , Shakeri-Manesch Soheila , Prager Gerhard , Wagner Oswald , Kautzky-Willer Alexandra , Clodi Martin , Luger Anton

Osteopontin (OPN) is a multifunctional glycoprotein implicated, among others, in bone metabolism, cardiovascular disease, diabetes and obesity. It promotes inflammation and insulin resistance. Osteopontin is elevated in the plasma and adipose tissue of obese subjects and decreases upon diet induced weight loss. Here we investigated the effect of bariatric surgery on plasma OPN concentrations in morbidly obese patients. 40 obese patients aged 43.1±1.8 years were recruited ...

ea0016p506 | Obesity | ECE2008

Cholinergic regulation of meal induced ghrelin and PYY release is impaired in obesity

Maier Christina , Riedl Michaela , Vila Greisa , Wolzt Michael , Clodi Martin , Ludvik Bernhard , Luger Anton

Ghrelin and PYY are gastrointestinal peptides involved in appetite regulation. The cholinergic part of the vagal nerve participate in the regulation of glucose and insulin levels. We aimed to examine the effects of the cholinergic antagonist atropine on ghrelin, PYY, glucose and insulin under basal conditions and after meal ingestion in lean and obese subjects. Eight lean and eight obese subjects received in a randomized, double-blind, placebo controlled crossover study design...

ea0016p507 | Obesity | ECE2008

Serum adiponectin levels in children with nonspecific infections: relations with fever and acute phase reactants

Aydin Banu , Kirel Birgul , Sirmagul Basar

It has been shown that adiponectin (AN) has antidiabetic, antiatherosclerotic and antiinflammatory activities. We analyzed serum AN levels in children with nonspecific infections. Forty-two prepubertal children (18 boys, 24 girls, age range: 14–163 months) admitted with fever ≥38 °C and nonspecific infection (upper respiratory tract infection, otitis, urine tract infections) symptoms were included. Their ideal body weights (IBW) for height were within 10...

ea0016p508 | Obesity | ECE2008

NCEP-ATP-III defined metabolic syndrome, type 2 diabetes mellitus and prevalence of hypogonadism in male patients with sexual dysfunction

Corona Giovanni , Mannucci Edoardo , Petrone Luisa , Balercia Giancarlo , Paggi Federica , Fisher Alessandra , Lotti Francesco , Chiarini Valerio , Forti Gianni , Maggi Mario

Objectives: Type 2 diabetes mellitus (T2DM) and metabolic syndrome (MetS) are characterized by insulin resistance and often associated with male hypogonadism. To discriminate the specific contribution of T2DM and MetS to male hypogonadism.Design and methods: A consecutive series of 1134 (mean age 52.1±13 years) male patients with sexual dysfunction was studied. Several hormonal and biochemical parameters were studied along with ANDROTEST, a 12-item ...

ea0016p509 | Obesity | ECE2008

Interleukin-1sz is a positive regulator of TIARP/STAMP2 in 3T3-L1 adipocytes

Sommer Grit , Kralisch Susan , Weise Sebastian , Ulrike Lossner , Matthias Bluher , Michael Stumvoll , Mathias Fasshauer

Tumor necrosis factor alpha-induced adipose-related protein (TIARP)/six-transmembrane protein of prostate 2 (STAMP2) has been characterized as an adipocyte-expressed protein which might play a crucial role in metabolic homeostasis and inflammatory pathways. In the current study, the impact of insulin resistance-inducing and proinflammatory interleukin (IL)-1ß on TIARP/STAMP2 gene expression was determined by quantitative real-time reverse transcription-polymerase chain re...

ea0016p510 | Obesity | ECE2008

Daily and nightly urinary free cortisol ratio as a marker of the hypothalamic-pituitary-adrenal (HPA) axis activity in abdominal obesity

Agostinio Romina Di , Vicennati Valentina , Pagotto Uberto , Genghini Silvia , Ambrosi Bruno , Maccario Mauro , Uberti Ettore Degli , Boscaro Marco , Cavagnini Francesco , Mantero Franco , Armanini Decio , Vettor Roberto , Marugo Mario , Pasquali Renato

Abdominal obesity (AO) might have a hyperactivation of the HPA axis but previous studies are limited by the small and heterogeneous number of patients investigated. The aim of this study was to evaluate urinary free cortisol (UFC) output during daily and nightly hours in a large cohort of AO women versus normal weight controls (CT). 107 AO women and 37 CT women were enrolled in this study; all subjects underwent a complete physical examination, an OGTT and biochemical determin...

ea0016p511 | Obesity | ECE2008

Impact of dietary management and diet duration on body weight and metabolic and endocrine changes in rats fed a high fat/low carbohydrate diet

Bielohuby Maximilian , Caton Samantha J , Spangler Lothar J , Bidlingmaier Martin

High-fat/low carbohydrate (HF–LC) diets in humans, such as the Atkin’s diet, are claimed to work in an ad libitum setting. In a previous study, we had demonstrated reduced bodyweight (BW) gain in rats when pair-fed isocaloric amounts of a HF–LC compared to normal chow (CH). However, rats on HF–LC also showed increased body fat and low serum IGF-I. We now compared BW development, food intake and resting energy expenditure (EE) in an ad libitum setting in mal...

ea0016p512 | Obesity | ECE2008

No feedback inhibition of obestatin during acylated ghrelin infusion in humans

Lucatello Barbara , St-Pierre David H. , Settanni Fabio , Olivetti Ilaria , Benso Andrea , Granata Riccarda , Ghigo Ezio , Broglio Fabio

Background: Obestatin is a 27 aa peptide derived from the ghrelin gene. Obestatin was first described to exert anorexigenic effects by decreasing gastric motility in rodent models, however these results have been debated and there is presently only small information regarding its activity and its regulation in humans. Furthermore, its interactions with acylated ghrelin (AG), derived from the same gene, have not been evaluated. Therefore, in these preliminary data, we sought to...

ea0016p513 | Obesity | ECE2008

Prevalence and characterization of metabolic syndrome (MS) in different classes of obesity and related endocrine factors

Genghini Silvia , Vicennati Valentina , Zampighi Adria , Corvaglia Stefania , Pasquali Renato

The aim of study was to evaluate in a group of obese women the prevalence of the metabolic syndrome, defined accordingly to the International Diabetes Federation, of single factors of the syndrome and the correlations with endocrine factors. We evaluated 351 women, 262 overweight-obese and 89 normal weight controls, which underwent physical, biochemical and hormonal evaluation. The prevalence of MS in obese and overweight subjects was 62.3%; the prevalence increased significan...

ea0016p514 | Obesity | ECE2008

Relationship among stress, weight gain and the activity of the hypothalamic-pituitary-adrenal (HPA) axis

Marconi Valentina , Vicennati Valentina , Corvaglia Stefania , Zampighi Adria , Agostino Romina Di , Pasquali Renato

The aim of this study was: 1) to determine whether there is a correlation between a stressful event, such as the mourning, the hyperactivity of HPA axis, and the fast weight gain 2) to assess whether there is a specific parameter that can predict the susceptibility of individuals to develop metabolic syndrome following a stressful event. We investigated 80 obese women, aged between 23 and 67 years; each subject underwent a clinical evaluation, an OGTT and biochemical, metaboli...

ea0016p515 | Obesity | ECE2008

Glucose-dependent insulinotropic polypeptide (GIP) receptor knock-out prevents ovariectomy-induced obesity in mice

Isken Frank , Pfeiffer Andreas FH , Nogueiras Ruben , Osterhoff Martin A , Ristow Michael , Thorens Bernard , Tschop Matthias H , Weickert Martin O

Objectives: Premature estrogen deficiency and menopause are associated with increases in body weight and fat mass. Ovariectomized (OVX) mice also show reduced locomotor activity. Because glucose-dependent-insulinotropic-polypeptide (GIP) is known to play an important role both in fat metabolism and locomotor activity, we hypothesized that effects of estrogen on the regulation of body weight, fat mass and spontaneous physical activity could be mediated in part by GIP signaling....

ea0016p516 | Obesity | ECE2008

Chronic central infusion of a ghrelin receptor (GHS-R1A) antagonist to rats: impact on ICV ghrelin-induced food intake and altered body composition

Salome N , Gustafsson L , Taube M , Hansson C , Karlsson L , Fehrentz JA , Martinez J , Perrissoud D , Dickson SL

Central treatment with ghrelin increases body weight and fat accumulation. Here we explore the pharmacological effect of a combined central (ICV) infusion of ghrelin (0.3 nmol/day) and a recently developed ghrelin receptor (GHS-R1A) antagonist (JMV2959, 100 nmol/day) on food intake and body composition. The effect of ghrelin infusion for 14 days to increase body weight (20.7±0.9% vs 34.3±3.1%, saline vs ghrelin, P<0.001) was blocked by co-infusion with JMV...

ea0016p517 | Obesity | ECE2008

Increased unsaturated fatty acids biosynthesis when pyruvate carboxylase is suppressed in adipocytes

Slawik Marc , Jitrapakdee Sarawut , Atherton Helen , Hadaschick Dirk , Kimber Wendy L , Considine Robert , Wallace John C , Sethi Jaswinder K , O'Rahilly Stephen , Griffin Julian L , Vidal-Puig Antonio

To maintain a proper lipid composition of cellular membranes is fundamental for cell viability and intracellular signalling. Therefore we hypothesised the existence of membrane quality control homeostatic mechanisms. Previously, we have shown that Pyruvate Carboxylase (PC), an enzyme involved in de novo lipogenesis and fuel partitioning, is a target of PPARγ and that PC level is decreased in PPARγ KO and obese insulin resistant mouse models. Here, we used RNAi...

ea0016p518 | Obesity | ECE2008

Glomerular filtration rates as a cardiometabolic risk markers in obese women

Bayraktaroglu Taner , Kutluturk Faruk , Dogan Azezli Adil , Orhan Yusuf

Introduction: This study carried out relationship between glomerulary filtration rates (GFR) and various metabolic parameters in obese women.Materials and methods: Selected subjects recruited retrospectively from outpatient clinic for this study were 5115 overweight or obese women with mean GFR which was 93 ml/dk/1.73 m2 calculated by MDRD (modification of diet in renal disease). According to mean GFR, they divided group I (low GFR) having &#8...

ea0016p519 | Obesity | ECE2008

In the metabolic syndrome adrenergic overdrive is independent on the obese state

Colombo Manuela Carla , Quarti-Trevano Fosca , Dell'Oro Raffaella , Gamba Pierluigi , Arenare Francesca , Perego Rita , Ilardo Valeria , Grassi Guido , Mancia Giuseppe

Objective: The metabolic syndrome (MS) is characterized by a marked sympathetic activation. Whether this adrenergic overdrive is mainly dependent on the obese state or it can be detected also in absence of obesity is unknown, however.Design and Methods: In 25 male patients (age 41.4±yrs., mean±S.E.M.) with MS (ATP III criteria) and in 12 age-matched healthy male controls, we measured body mass index (BMI), waist circumference (WC...

ea0016p520 | Obesity | ECE2008

Changes of the testosterone levels in young males with obesity and metabolic syndrome

Robeva Ralitza , Kirilov Georgi , Tomova Analia , Kumanov Philip

Aim: The present study aimed to compare the androgen levels in men with obesity, metabolic syndrome /MS/ and healthy normal – weight controls.Materials and methods: Eighty-six males (mean age 29.65±8.57 /18–50/) were investigated. 31 of them were healthy (mean BMI – 23.66±1.97); 22 were with simple obesity (mean BMI – 32.79±7.16) and 33 (mean BMI – 35.78±8.71) were with overt metabolic syndrome /MS/ according ...

ea0016p521 | Obesity | ECE2008

Expression of adrenergic receptors and atrial natriuretic peptide in human adipose tissue of severe obese women and Rosiglitazone action during differentiation of human pre-adipocytes

Garruti Gabriella , Giusti Vittorio , Nussberger Jurg , Darimont Christian , Appert-Collin Aline , Perrini Sebastiano , NennigerTosato Monique , Giorgino Riccardo , Giorgino Francesco , Cotecchia Susanna

Background: The balance between Adrenergic Receptor (AR) subtypes and Atrial Natriuretic Peptide (ANP) is crucial for lipolysis. It was previously demonstrated that ANP is strongly lipolytic and we recently demonstrated that ANP is expressed and secreted by human pre-adipocytes. In previous studies, β-receptor density and lipolysis were higher in human visceral adipose tissue (HVAT) as compared with human subcutaneous adipose tissue (HSAT).Aims and ...

ea0016p522 | Obesity | ECE2008

Glucose-dependent insulinotropic polypeptide downregulates expression and enzyme activity of 11β-Hydroxysteroid Dehydrogenase type 1

Andres Janin , Gogebakan Ozlem , Mai Knut , Pfeiffer Andreas , Spranger Joachim

Introduction: 11β-hydroxysteroid dehydrogenase type 1 (11 β-HSD1) converts cortisone into its active metabolite, cortisol. Mechanisms regulating the promoter-activity of 11β-HSD1 are of considerable importance to understand the basics of intracellular cortisol, lipid and glucose metabolism. The incretine glucose-dependent insulinotropic polypeptide (GIP) has been suggested to affect insulin sensitivity. However, the mechanisms of this effect are unclear yet....

ea0016p523 | Obesity | ECE2008

Obesity in GDM: a registry of GDM in Portugal

Ruas Luisa , Paiva Sandra , Rocha Tiago , Dores Jorge , Carvalheiro Manuela

Introduction: A retrospective study of the year 2003, of 1314 women with GDM, from 24 public health Centres in Portugal, was performed.Patients and methods: Women were divided into two groups according to their pre-pregnancy BMI: group Go – BMI ≥30 kg/m2 and group Gno BMI <30 kg/m2. The mean age of these women was 32.9±5 years (18–45), the A1c was <6% in both groups. The influence of the BMI in different...

ea0016p524 | Obesity | ECE2008

Sleep loss and metabolic response to breakfast

Schmid Sebastian M , Jauch-Chara Kamila , Hallschmid Manfred , Wilms Britta , Born Jan , Schultes Bernd

Background: Sleep loss is increasingly common in the western world and has been shown to be associated with an increased risk of diabetes mellitus and obesity.Objective: We hypothesised that short term sleep loss results in a diabetogenic metabolic status during carbohydrate challenge at a breakfast buffet.Subjects and methods: Fifteen healthy, normal-weight young men were studied in randomized balanced order on the subsequent morn...

ea0016p525 | Obesity | ECE2008

Abdominal fat and atheromatosis risk factors in non-obese healthy subjects

Bednarek-Tupikowska Grazyna , Kubicka Eliza , Tworowska-Bardzinska Urszula , Lenarcik Agnieszka , Matczak-Giemza Magdalena , Milewicz Andrzej , Krzyzanowska-Swiniarska Barbara , Katra Barbara

In the 1980s the concept, that some individuals with normal weight (Metabolically Obese, Normal-Weight) have disturbances of metabolism, related to obesity was proposed. It is known, that obesity promotes atheromatosis by various mechanism, inter alia due to excess of inflammatory markers. This problem however, was not investigated in non-obese subjects.We analyzed anthropometric variables, body fat distribution by DXA and serum CRP, Il-6 and Il-18 in 43...

ea0016p526 | Obesity | ECE2008

The effect of fasting on substrate metabolism and growth hormone signaling

Moller Louise , Dalman Lisa , Norrelund Helene , Billestrup Nils , Otto Jorgensen Jens

Background: Fasting is associated with suppression of IGF-I production, which is likely to be the stimulator of GH secretion and the subsequent increase in lipolysis. The GH signalling events subserving this switch in the actions of GH have not been studied.Aim: To assess whole body substrate metabolism and GH signalling proteins in skeletal muscle and fat in healthy human subjects in the postabsorptive state and after 38 h of fasting.<p class="abste...

ea0016p527 | Obesity | ECE2008

Regulation of acylation stimulating protein by insulin in overweight and obese postmenopausal women: a MONET study

St-Pierre David H , Cianflone Katherine , Smith Jessica , Lu Huiling , Coderre Lise , Karelis Antony D , Imbeault Pascal , Lavoie Jean-Marc , Rabasa-Lhoret Remi

Objective: Acylation Simulating Protein (ASP) has been shown to regulate lipid clearance and glucose uptake in adipose tissue. In vitro studies demonstrate that insulin stimulates ASP secretion from adipocytes. Individuals with obesity and/or metabolic disturbances (insulin resistance and type 2 diabetes) have increased plasma ASP, suggesting ASP resistance. Therefore, the present study evaluates whether ASP levels are influenced by the metabolic profile of overweight and obes...

ea0016p528 | Obesity | ECE2008

Prevalence of obesity and overweight in Iranian middle aged adults

Houshiar Rad Anahita , Abdollahi Morteza , Esmaeili Mina

Introduction: High prevalence of obesity and overweight among middle aged adults is a major health problem. It is related to high prevalence of many chronic degenerative diseases in this high risk group. This study was conducted to determine the prevalence of obesity and overweight among young and middle aged Iranian adults.Methods: This study was based on anthropometric data collected in the national household food consumption and nutritional status sur...

ea0016p529 | Obesity | ECE2008

Determination of dimerisation domains of the human melanocortin 4 receptor

Genetzky Jessica , Tarnow Patrick , Rediger Anne , Gruters Annette , Biebermann Heike

The melanocortin 4 receptor (MC4R), a G protein coupled receptor, has a prominent role in hypothalamic weight regulation. The MC4R was recently shown to form receptor dimers. The cannabinoid 1 receptor (CB1R) is the nearest phylogenetic relative of MC4R but fails to interact with the MC4R in vitro. In this study we aim to investigate receptor domains that are necessary for MC4R interaction. Four different chimeric receptors of MC4R and CB1R were constructed. The generated cons...

ea0016p530 | Obesity | ECE2008

Fluctuation of orexin, ghrelin and melatonin levels during daytime

Racz Beata , Duskova Michaela , Vondra Karel , Simunkova Katerina , Nedvidkova Jara , Sedlackova Dana , Hill Martin , Starka Luboslav

Introduction: Melatonin plays a key role in the circadian timing system. The question remains whether changes in endogenous melatonin may be associated with food intake. Orexin and ghrelin are involved in the regulation of energetic homeostasis. Therefore, we decided to obtain more detailed data on the circadian changes of orexin, ghrelin and melatonin for identification of the changes in these hormones to food intake and day-time, together with glycemic and C-peptide levels.<...

ea0016p531 | Obesity | ECE2008

Metabolic syndrome and endothelial dysfunction in women with polycystic ovary syndrome – relationship with insulin sensitivity

Kowalska Irina , Kozlowska Agnieszka , Straczkowski Marek , Karczewska-Kupczewska Monika , Adamska Agnieszka , Nikolajuk Agnieszka , Wolczynski Slawomir , Gorska Maria

Polycystic ovary syndrome (PCOS) is a main cause of women infertility. There are data suggesting an increased risk for cardiovascular disease in PCOS. Insulin resistance might play a role in the pathogenesis of PCOS and its metabolic complications. The aim of the study was to asses the prevalence of The National Cholesterol Education Program (NCEP)-defined metabolic syndrome (MS) in women with PCOS in relation to insulin resistance and endothelial dysfunction – soluble E-...

ea0016p532 | Obesity | ECE2008

Diabetogenic effects of a continuous infusion of acylated ghrelin in normal and type 2 diabetic patients

St-Pierre David H , Olivetti Ilaria , Pomero Elisa , Gramaglia Elena , Riganti Fabrizio , Benso Andrea , Ghigo Ezio , Broglio Fabio

Background: Previous studies have shown that acylated ghrelin (AG) modulates insulin secretion and glucose disposal in vitro and after acute administration in humans. The aim of the present study was to evaluate postprandial glucose and insulin response following AG infusion in normal subjects and patients with type 2 diabetes.Methods: Six normal subjects and 3 patients with type 2 diabetes were included in this study. AG (1 μg/kg per h) or s...

ea0016p533 | Obesity | ECE2008

Pentraxin 3 production in human visceral adipose tissue is associated with cardiovascular risk factors

Alberti Luisella , Gilardini Luisa , Girola Andrea , Micheletto Giancarlo , Peri Giuseppe , Doni Andrea , Mantovani Alberto , Invitti Cecilia

Pentraxin 3 (PTX3) is an acute phase protein expressed in the advanced atherosclerotic lesions and produced by endothelial cells, macrophages and, in rodents also by adipocytes under tumour necrosis factor α (TNFα) exposure. We investigated if PTX3 is expressed in human adipose tissue and is associated with cardiometabolic risk factors.Methods: Samples of subcutaneous (SAT) and omental visceral (VAT) adipose tissue were obtained from 21 obese (...

ea0016p534 | Obesity | ECE2008

Insulin and glucose regulates omentin-1, a novel adipokine: relation to women with the polycyastic ovary synfrome

Tan Bee K , Adya Raghu , Farhatullah S , Lewandowski KC , O'Hare Paul , Lehnert Hendrik , Randeva Harpal S

Polycystic ovary syndrome (PCOS) is associated with insulin resistance and obesity. Recent studies have shown that plasma omentin-1 levels decrease with obesity. Currently, no data exists on the relative expression and regulation of omentin-1 in adipose tissue (AT) of PCOS women.Objectives: To assess mRNA and protein levels of omentin-1 in omental (om) AT of PCOS women and matched controls, including circulating omentin-1. Ex vivo and in vivo</...

ea0016p535 | Obesity | ECE2008

Postnatal determination of ghrelin secretion levels and sexual dimorfism in the rat

Al-Massadi Omar , Gonzalez Carmen Ruth , Pardo Maria , Dieguez Carlos , Casanueva Felipe , Seoane Luisa Maria

Ghrelin is a pleiotropic hormone characterized mainly by its strong orexigenic action and its role as GH secretagogue.Age-related level variation of this hormone has been previously shown in different studies centred in mARN expression and in its plasma levels; however there is no data about its secretion directly from the stomach although this is the main ghrelin producing organ.Objectives: To determine the levels of ghrelin in th...

ea0016p536 | Obesity | ECE2008

The effect of polyunsaturated fatty acids enriched eggs on lipid profile and inflammatory markers

Manda Dana , Giurcaneanu Mihaela , Ionescu Liliana , Criste Rodica , Panaite Tatiana , Vladoiu Suzana , Busu Carmina , Ianas Olga

The correction of a subtle nutritional deficiency that may reduce the risk of a future chronic disease is indeed a challenge. One of the most intriguing current and future impacts on public health may come from a greater intake of omega-3 fatty acids such as α-linolenic acid (ALA).Objective: We investigated the effect of an increased amount of dietary α-linolenic acid (ALA) from enriched eggs on the lipid profile and inflammatory markers in hea...

ea0016p537 | Obesity | ECE2008

Carbohydrate and lipid oxidation in relation to serum adiponectin concentration in women with anorexia nervosa

Karczewska-Kupczewska Monika , Kowalska Irina , Straczkowski Marek , Adamska Agnieszka , Nikolajuk Agnieszka , Otziomek Elzbieta , Gorska Maria

Anorexia nervosa (AN) is an eating disorder, with the significant loss of the adipose tissue. Lack of subcutaneous adipose tissue observed in lipodystrophies is accompanied by insulin resistance. The crucial step in the development of insulin resistance is an impaired increase in carbohydrate oxidation and decrease in lipid oxidation in response to insulin. The aim of the present study was to estimate carbohydrate and lipid oxidation in relation to serum adiponectin concentrat...

ea0016p538 | Obesity | ECE2008

Molecular insights in dysfunctions of the human melanocortin-4-receptor (MC4R) caused by mutations in the third transmembrane domain (TM3) and the second intracellular loop

Tarnow Patrick , Rediger Anne , Widhalm Kurt , Friedel Susann , Kleinau Gunnar , Bolz Hanno , Bettecken Thomas , Hinney Anke , Krause Gerd , Gruters Annette , Biebermann Heike

Mutations in the hypothalamic expressed MC4R gene are the most frequent cause of monogenetic obesity. This Gαs-Protein coupled receptor (GPCR) is activated by endogenous ligands α- and β-MSH and is inhibited by the only known endogenous inverse agonist and antagonist Agouti related peptide (AgRP). Naturally occurring mutations help to understand activation mechanisms of the human MC4R.We previously described a constitutively act...

ea0016p539 | Obesity | ECE2008

Ghrelin gene polymorphisms in Prader Willi Syndrome

Prodam Flavia , Bellone Simonetta , Corneli Ginevra , Rienzo Francesca Di , Giacoma Sara , Rapa Anna , Vivenza Daniela , Grugni Graziano , Crino Antonino , Battista Eliana Di , Bona Gianni

Introduction: Prader Willi Syndrome (PWS) is a genetic syndrome characterized by hyperphagia, morbid obesity, and many other endocrine alterations. PWS subjects present higher ghrelin levels. The cause of this increase as well as the modulation of ghrelin secretion at fasting and feeding in relation to other metabolic parameters in PWS is largely unknown. It has also been demonstrated that many ghrelin gene (GHRL) polymorphisms are associated with obesity, type 2 diabetes, and...

ea0016p540 | Obesity | ECE2008

Retinol-binding protein 4 is independently associated with insulin resistance in PCOS women

Mohlig Matthias , Weickert Martin O , Ghadamgahi Elham , Arafat Ayman M , Spranger Joachim , Pfeiffer Andreas FH , Schofl Christof

Objective: Adiposity, insulin resistance, and hyperandrogenism are features of the polycystic ovary syndrome (PCOS). Retinol-binding protein 4 (RBP4) secreted from adipose and liver tissue has been linked to insulin resistance. We here address the impact of RBP4 on insulin resistance in PCOS and its usability to identify women with metabolic syndrome or impaired glucose metabolism (IGT or diabetes).Design: RBP4 was determined in plasma of 115 PCOS...

ea0016p541 | Obesity | ECE2008

Glucagon suppression of total but not of acylated ghrelin is preserved in obesity: the impact on appetite control

Arafat Ayman M , Adamidou Aikaterini , Otto Barbel , Weickert Martin O , Perschel Frank H , Spranger Joachim , Schofl Christof , Mohlig Matthias , Pfeiffer Andreas F H

Objective: The mechanisms underlying the well known glucagon-induced satiety effect are unclear. As we showed recently, the glucagon-induced reduction in total ghrelin, that might be responsible for this effect, is exerted at hypothalamus–pituitary level. The aim of the present study was to further evaluate glucagon’s suppressive effect on both total and acylated-ghrelin in obesity with respect to its role in appetite control.Methods: Prospecti...

ea0016p542 | Obesity | ECE2008

The sirtuin activating compound resveratrol decreases GH transcription

Monteserin Jose Luis , Stalla Johanna , Stalla Gunter , Theodoropoulou Marily

Caloric restriction is the best known experimental procedure that extends lifespan and resistance to aging-related diseases. Part of these actions was found to be mediated by the NAD-dependent histone deacetylase Sir2/Sirt1. Resveratrol is a polyphenol found in the skin of grapes, which was identified as a Sirt1 activator and subsequently proposed as a caloric restriction mimetic. The aim of the present study was to determine how resveratrol affects the GH axis, which is centr...

ea0016p543 | Obesity | ECE2008

Glucose-dependent insulinotropic polypeptide (GIP) modulates inflammatory markers and regulates cell proliferation in subcutaneous adipose tissue

Gogebakan Ozlem , Mosig Sandy , Middelbeck Ines , Osterhoff Martin A , Isken Frank , Rudovich Natalia , Pfeiffer Andreas F H

Background: Glucose-dependent insulinotropic polypeptide (GIP) is a gastrointestinal hormone that is secreted in response to food intake and modulates β-cell function. Remarkably, GIP looses its insulinomimetic effect in the presence of chronic hyperglycaemia and thus appears to be ineffective in poorly controlled T2DM at least with regard to insulin release. The mechanism by which this occurs is not clear and it is not known.Objectives: To analyse ...

ea0016p544 | Obesity | ECE2008

Serum soluble E-selectin concentration in relation to insulin resistance and metabolic inflexibility in obese women

Straczkowski Marek , Kowalska Irina , Adamska Agnieszka , Nikolajuk Agnieszka , Karczewska-Kupczewska Monika , Gorska Maria

Markers of endothelial dysfunction, including soluble E-selectin (sE-selectin) are related to insulin resistance and are predictors of type 2 diabetes. Insulin resistance is associated with metabolic inflexibility, i.e. an impaired stimulation of carbohydrate oxidation and inhibition of lipid oxidation in insulin-stimulated conditions. The aim of the present study was to estimate the relationships of serum sE-selectin concentration with carbohydrate and lipid oxidation in lean...

ea0016p545 | Obesity | ECE2008

The volume of left hepatic lobe as metabolic marker, correlates with weight and not with height, both in obese or non-obese women and men

Peretianu Dan , Carsote Mara , Staicu Daniela Cristea , Poiana Catalina

Introduction: Left hepatic lobe volume (LHLV) has been associated with hyperinsulinemia and insulin resistance in obese women.Aim: To study the correlation between LHLV and body weight, regardless the weight and gender of the patient.Subjects and methods: The left hepatic lobe was evaluated with an echographic probe of 3.5 MHz. The LHLV (cm3) was calculated with the formula: antero-posterior diameter (length)×longit...

ea0016p546 | Obesity | ECE2008

Decreased NK cell functions from obese F344 rats can be altered after transfusion in lean littermates

Nave Heike , Lautenbach Anne , Muller Gunther , Jacobs Roland , Siegmund Britta , Wrann Christiane , Brabant Georg

Leptin acts not only as an anorexigenic hormone but is also involved in the regulation of cell-mediated immunity. However, since the impact of leptin on NK cells is currently elusive, we investigated the body weight-dependent leptin effects on NK cells numbers and function.In a first set of experiments leptin and MADB106 tumor cells were injected intravenously in male lean and diet-induced obese Lewis and F344 rats. In a second set of experiments an i...

ea0016p547 | Obesity | ECE2008

Central resistin regulates both hypothalamic and peripheral lipid metabolism in a nutritional dependent fashion

Vazquez MJ , Gonzalez CR , Varela L , Lage R , Tovar SA , Sangiao-Alvarellos S , Williams LM , Vidal-Puig A , Nogueiras R , Lopez M , Dieguez C

Current evidence suggests that the adipocyte-derived hormone resistin (RSTN) regulates both feeding and peripheral metabolism through unclear hypothalamic-mediated mechanisms. Here, we demonstrate by the first time, that the anorectic effect of RSTN is associated to specific changes in the expression of neuropeptides in the arcuate nucleus of the hypothalamus (ARC), namely AgRP, NPY and CART. Very interestingly, RSTN also exerts a deep, nutritional-dependent inhibitory effect ...

ea0016p548 | Obesity | ECE2008

Changes in physical activity and changes in body weight: a longitudinal assessment in the SUN dynamic cohort

Basterra-Gortari Francisco Javier , Bes-Rastrollo Maira , Forga Lluis , Martinez-Gonzalez Miguel Angel

Objective: To evaluate the associations between baseline leisure-time physical activity (LTPA) or changes in LTPA during follow-up on long-term weight changes, and to investigate the effects of physical activity in participants with baseline BMI<25 kg/m2 and baseline BMI≥25 kg/m2.Material and methods: Data of the prospective dynamic cohort (SUN cohort) were used. In total 12 117 participants, all of them university graduat...

ea0016p549 | Obesity | ECE2008

The relationship between alteration in plasma inflammation, metabolic biomarkers and severe steatosis in a group of morbidly obese patients

Sirbu Anca , Barbu Carmen , Botusan Ileana , Arbanas Tudor , Albu Alice , Fica Simona

Aim: The aim of the study was to evaluate the relation between anthropometric measurements, plasma biomarkers of inflammation, metabolic and adipokines pattern and the presence of severe steatosis, in a group of patients with morbid obesity.Materials and methods: We evaluated a group of 40 patients (25 F, 15 M) with morbid obesity and indication for bariatric surgery. Anthropometric measurements (waist circumference, BMI determination) were performed and...

ea0016p550 | Obesity | ECE2008

Endocrine and metabolic alterations in narcoleptic patients as result of orexin-A reduction

Dalmazi Guido , Poli Francesca , Ribichini Danilo , Fanelli Flaminia , Vicennati Valentina , de Iasio Rosaria , Montagna Pasquale , Pasquali Renato , Plazzi Giuseppe , Pagotto Uberto

Aims: Narcolepsy with cataplexy (NC) is a rare, chronic disease, characterized by excessive daytime sleepiness, cataplexy, disrupted nocturnal sleep and manifestations of abnormal REM (Rapid Eye Movement) sleep. Human NC is probably caused by environmental and genetic factors, leading to dysfunctions in hypothalamic orexigenic neurons. Recent studies showed that NC was associated with an increase in Body Mass Index, despite a reduction in food intake. In the current study we i...

ea0016p551 | Obesity | ECE2008

Prevalence of non alcoholic steatohepatitis in diabetic patients with morbid obesity

Segovia Raquel , Camara Rosa , Del Olmo Maribel , Ponce Jose , Navas Soledad , Argente Maria , Pinon Francisco

Non alcoholic steatohepatitis (EHNA) is diagnosed by hepatic biopsy. Its etiology is uncertain but obesity is present in 69–100% of all cases and type 2 diabetes mellitus (DM) in about 34–75%.Objectives: To study the prevalence of EHNA in morbidly obese patients and determine if there is a difference between the prevalence in patients with and without carbohydrate metabolism disorders.Patients and methods: Prospective stu...

ea0016p552 | Obesity | ECE2008

Dual effect of the adapter Grb14 on insulin action in primary mouse hepatocytes

Carre Nadege , Cauzac Michele , Girard Jean , Burnol Anne-Francoise

Obesity and type 2 diabetes are expanding rapidly and become a wide world health issue. These metabolic diseases are tightly associated with an insulin resistance state. Among insulin target tissues, liver plays a central role in the regulation of glucose homeostasis.Insulin action is initiated by its binding to its receptors (IR) which, once activated, phosphorylate intracellular substrates and lead to the activation of transduction pathways implicating...

ea0016p553 | Obesity | ECE2008

Consequences of the modification of Grb14 expression level on adipocyte differentiation and metabolism

Goenaga Diana , Moldes Marthe , Holt Lowena , Daly Roger , Girard Jean , Burnol Anne-Francoise

Grb14 is a molecular adaptor that inhibits insulin signaling by interacting with the activated insulin receptor and inhibiting its tyrosine kinase activity. In various models of insulin resistance, including obese mice and type 2 diabetes patients, an inverse correlation was reported between Grb14 expression in adipose tissue and insulin sensitivity. To elucidate Grb14 function in this specific tissue, we studied the impact of modifications of Grb14 level of expression on adip...

ea0016p554 | Obesity | ECE2008

25-Hydroxyvitamin D deficiency in premenopausal morbidly obese women

Galiana Pablo Abellan , Gomez Rosa Camara , del Olmo Garcia Maria Isabel , Marco Jose Luis Ponce , Torres Juan Francisco Merino , Alborg Vicente Campos , Selles Francisco Pinon

Background: Obesity has been associated with low levels of 25-hydroxyvitamin D (25(OH)D). This could be explained by a low dietary intake, reduced sun exposure or less biodisponibility because of its arrest in the fat mass. The main objective is to study the prevalence of 25(OH)D deficiency in premenopausal obese women.Methods: Aleatory sample of 100 morbidly obese patients. Selection criteria were: women, premenopausal, Caucasian. Patient with diabetes ...

ea0016p555 | Obesity | ECE2008

Association study on three single nucleotide polymorphisms upstream and in the GIPR in obese and lean children from Berlin

Sauber Jeannine , Jyrch Sabine , Bronner Gunter , Friedel Susann , Illig Thomas , Grallert Harald , Hebebrand Johannes , Wiegand Susanna , Krude Heiko , Gruters Annette , Brumm Harald , Biebermann Heike

Introduction: In the past 20 years, obesity has become a major health problem occurring as well in adults as in children. Beside environmental influences on body weight, the genetic background of a person plays an important role in body weight control. After the description of monogenetic mutations linked to obesity most current studies investigate polygenetic forms of obesity.Recent studies showed an association between two SNPs, located in the promoter...

ea0016p556 | Obesity | ECE2008

Screening for mutations in the glucose-dependent insulinotrophic polypeptide receptor gene (GIPR) in obese patients with disturbed glucose tolerance

Behm Maria , Ambrugger Petra , Sauber Jeannine , Hinney Anke , Hebebrand Johannes , Friedel Susann , Bronner Gunter , Wiegand Susanna , Krude Heiko , Gruters Annette , Biebermann Heike

Objective: The increasing incidence of obesity is a major health problem world-wide. So far, mutations were identified in genes encoding major contributors in energy homeostasis. With the exception of mutations in the melanocortin-4-receptor gene (MC4R) obesity-causing mutations are very rare. To date great efforts were undertaken to identify gene variants that contribute to polygenic obesity. The GIPR belongs to the large superfamily of G-protein coupled recepto...

ea0016p557 | Obesity | ECE2008

Analysis of the central glucocorticoid feedback in patients with obesity, diabetes, and depression

Greiling Marie , Hubold Christian , Conrad Matthias , Kuhn Johanna , Fischer Bernd , Lehnert Hendrik , Peters Achim

Obesity, diabetes, and depression are associated with distinct neuroendocrine changes influencing the regulation of the hypothalamus pituitary adrenal (HPA) system. Up to now it was not possible to examine the central glucocorticoid feedback in humans to verify results of animal experiments in relating disease models. With the help of a recently developed computational approach we were enabled to address this question to patients with obesity, diabetes, or depression.<p cl...

ea0016p558 | Obesity | ECE2008

Evaluation of metabolic syndrome in patients with diabetes mellitus type 2 using different criteria: National Cholesterol Education Program's Adult Treatment Panel III (ATPIII) and International Diabetes Federation (IDF)

Bajkin Ivana , Stokic Edita , Radovanov Tijana , Tomic-Naglic Dragana , Tomic Dusan , Munjiza Milena , Crnobrnja Veljko

The aim of the study was to compare ATPIII and IDF criteria for metabolic syndrome in diabetes mellitus type 2 subjects.Two hundred and seventy-seven participants were included, 177 women 60.43±8.06 years and 100 men aged 56.35±9.58 years. Group A: metabolic syndrome by ATPIII; group B metabolic syndrome by IDF.Following laboratory and antropometric measurements were done: total cholesterol, triglycerides, LDL- and HDL ch...

ea0016p559 | Obesity | ECE2008

Relation of high-sensitive C-reactive protein and metabolic risk factors in overweight and obese women

Kutluturk Faruk , Bayraktaroglu Taner , Azezli Adil , Orhan Yusuf

Introduction: Elevated high-sensitive c-reactive protein (hs-CRP) in association with hyperinsulinemia, obesity, insulin resistance and high blood pressure are significant risk factors for cardiovascular diseases. The aim of this study is to evaluated the correlations between serum hs-CRP concentration and metabolic risk factors in overweight and obese women.Methods: Overweight (BMI>25 kg/m2) and obese (BMI>30 kg/m2) 755 wom...

ea0016p560 | Obesity | ECE2008

Relationship of visceral adiposity with plasma adiponectin concentration: effect of weight loss

Nasseri Esmat , Hosseini Mostafa , Dorosti Ahmad Reza , Chamari Maryam

Objective: To investigate the associations between adiponectin levels and adiposity and body fat distribution indices assessed by waist to hip ratio in Iranian women and to determine if the association differ as a result of ethnicity.Methods: A cross-sectional study of 76 Iranian women free from metabolic disease was performed. Body mass index (BMI), waist circumferences, waist to hip ratio (WHR), body composition, insulin sensitivity, lipid profiles and...

ea0016p561 | Obesity | ECE2008

The interrelationship between leptin, visfatin and CRP levels and insulin sensitivity assessed by euglycaemic clamp in obese women

Micic Dragan , Sumarac-Dumanovic Mirjana , Stamenkovic-Pejkovic Danica , Simic Maja , Cvijovic Goran , Zoric Svetlana , Kendereski Aleksandra , Stevanovic Darko , Jorga Jagoda , Trajkovic Vladimir

Visfatin is a newly discovered adipokine found in abundance in visceral fat. Leptin is well known marker of fat mass in the body but also one aspect of leptin is its action as a proinflammatory cytokine. Recent studies, both in vitro and in vivo, indicated that visfatin is also one of the inflammatory cytokines, although the relationship between visfatin and insulin resistance remains still unclear. The aim of our study was to assess the association between visfa...

ea0016p562 | Obesity | ECE2008

Obesity and adipocytokines

Gorar Suheyla , Culha Cavit , Demir Yavuz S , Turgut Ahmet T , Karakaya Pinar , Serter Rustu , Aral Sema , Aral Yalcin

Aim: Comparison of the parameters of obesity with the newly-defined adipocytokines in females applying to our clinic for obesity.Method: Demographical data of 36 obese females were determined. Cases were classified as obese and morbid obese according to BMI values. Biochemical parameters were measured for all the cases. Thicknesses of subcutaneous and visceral fatty tissues were measured on ultrasonography. Statistical significances were evaluated by app...

ea0016p563 | Obesity | ECE2008

ERK1/2 MAPKs and Wnt signaling pathways are independently involved in adipocyte-mediated aldosterone secretion

Vleugels Kim , Ehrhart-Bornstein Monika , Bornstein Stefan R , Krug Alexander W

One important risk factor for the development of arterial hypertension is abdominal obesity. Aldosterone, secreted by adrenocortical cells, promotes sodium and water retention and by that regulates blood pressure homeostasis. Increased serum aldosterone levels have been linked to the development of obesity hypertension. Therefore, identifying the link between obesity and increased aldosterone secretion is of high importance for the management of obesity hypertension.<p cla...

ea0016p564 | Obesity | ECE2008

Leptin decreases and glucose deprivation increases the generation of the soluble leptin receptor (sOb-R) in a cell model

Schaab Michael , Morawe Daniel , Klammt Jurgen , Wilfert Wolfgang , Thiery Joachim , Kratzsch Jurgen

Objective: The soluble leptin receptor (sOb-R) is generated by ectodomain shedding of the membrane-associated Ob-R and may modulate leptin action in metabolic dysregulations. The objective of our study was to investigate whether or not sOb-R generation may be induced by indicators of energy availability or metabolic regulators in a cell model.Material and methods: The four human Ob-R isoforms were cloned, characterized by binding experiments with 125-iod...

ea0016p565 | Obesity | ECE2008

The impact of middle term feeding with organic, low input and conventional diets on body composition and plasma leptin concentration in male rats

Srednicka Dominika , Krolikowski Tomasz , Gromadzka-Ostrowska Joanna , Rembialkowska Ewa , Lueck Lorna , Leifert Carlo

The aim of the study was to analyze the influence of feed from different production systems on food intake, body chemical composition and plasma leptin concentrations in rats. It is expected, that organically produced feed influences positively rat organism, in that hormone balance. Leptin regulates energy intake and expenditure, as well as basic functions of many tissues.The experiment was conducted in 104 Wistar male rats divided into 16 dietary groups...

ea0016p566 | Obesity | ECE2008

Prospective, Placebo controlled, randomized treatment of 67 obese children/adolescents with metformin

Wiegand Susanna , L'Allemand Dagmar , Huebel Hanna , Burmann Mareike , Krude Heiko , Gruters Annette , Holl Reinhard

Background: It becomes increasingly evident that features of the metabolic syndrome are already present in young obese patients. Especially an impaired glucose metabolism with insulin resistance is an alarming sign of existing comorbidity of childhood obesity. Metformin has been argued as one pharmacological option to improve impaired glucose metabolism at least in obese adults. So far three prospective randomized studies were performed in childhood cohorts with <30 patien...

ea0016p567 | Obesity | ECE2008

Hyperandrogenism in pre- and post-menopausal obese women

Ferreira Joana , Souto Selma , Oliveira Ana , Freitas Paula , Correia Flora , Varela Ana , Carvalho Davide , Medina Jose Luis

Introduction: Obesity has been associated with increased androgenicity in women, however there are conflicting data on this subject. Objectives: To determine whether any androgen is independently related to body mass index (BMI), waist-to-hip ratio (WHR) or age. To access if there is an association between elevated plasma androgen levels and the presence of either morbid obesity or metabolic syndrome (MS).Design and Methods: A total of 148 obese women we...

ea0016p568 | Obesity | ECE2008

Relationship between obesity, IL-17 and IL-23 and insulin resistance

Sumarac-Dumanovic Mirjana , Micic Dragan , Simic Maja , Stamenkovic-Pejkovic Danica , Stevanovic Darko , Jorga Jagoda , Cvijovic Goran , Zoric Svetlana , Trajkovic Vladimir

In most obese patients, obesity is associated with a low-grade inflammation of white adipose tissue resulting from chronic activation of the innate immune system and which can subsequently lead to insulin resistance, impaired glucose tolerance and even diabetes. It is still not clear role of acquired immune response in obesity and related comorbidites such as asthma, cancer or autoimmune diseases. The aim of our study was to compare level s of IL-17 and IL23 in sever obese wom...

ea0016p569 | Obesity | ECE2008

NMR study of ghrelin and desacyl-ghrelin with the growth hormone releasing receptor type 1a using living cells

Pazos Yolanda , Martin-Pastor Manuel , De Capua Antonia , Alvarez Carlos J P , Casanueva Felipe F

Ghrelin, the endogenous ligand for the GH secretagogue receptor type 1a (GHS-R1a), is a peptide with a post-translational octanoyl modification on Ser-3. Previous studies of ghrelin derivatives showed that the pendant group at Ser-3 plays an essential role in the bioactivity. The acylated peptide specifically releases growth hormone (GH) both in vivo and in vitro, while the desoctanoyl form of the hormone is at least 100-fold less potent than the parent peptide.<p class="a...

ea0016p570 | Obesity | ECE2008

C-reactive protein and insulin sensitivity in severe obese women with and without metabolic syndrome

Simic Maja , Micic Dragan , Sumarac-Dumanovic Mirjana , Stamenkovic-Pejkovic Danica , Jorga Jagoda , Cvijovic Goran , Kendereski Aleksandra , Zoric Svetlana

It was postulated that different therapeutic approaches could influence the markers of inflammation in obesity but it is unknown whether overweight women with and without metabolic syndrome have the same degrees of inflammatory state. The aim of our study was to compare level of hs-CRP in obese women without other components of metabolic syndrome with hs-CRP in women with metabolic syndrome (according IDF criteria). Overweight body mass index-matched women without (OW)) (n<...

ea0016p571 | Obesity | ECE2008

Inflammatory parameters in obesity: lack of influence of weight loss

Sola Eva , Jover Ana , Garcia Katherinne , Salvador Carlos , Morillas Carlos , Hernandez-Mijares Antonio

Introduction: Obesity has been associated with a low-level activation of the acute-phase response. The aim of the present study was to compare inflammatory parameters in patients with obesity and a control group, and to evaluate the effect of weight loss on these parameters.Methods: Sixty-seven severe or morbid obese patients (51 women, 16 men), aged 34±11 years, were compared with 67 normal-weight controls (45 women, 22 men), aged 32±10 years....

ea0016p572 | Obesity | ECE2008

Activated protein C levels in obesity and weight loss influence

Sola Eva , Espana Francisco , Vaya Amparo , Estelles Amparo , Garcia Katherinne , Hernandez-Mijares Antonio

Introduction: Several haemostatic disturbances have been described in obese patients. Nevertheless, the state of coagulation inhibitors has been scarcely studied in these patients. Activated protein C (APC) is the anticoagulant enzyme formed upon activation of the protein C (PC) by the thrombin-thrombomodulin complex on the surface of endothelial cells. Morbid obesity is associated to endothelial dysfunction, which could cause a reduction in APC generation.<p class="abstex...

ea0016p573 | Obesity | ECE2008

Determination of obesity and its relationship with hypertension among Semnanian adults, Iran

Pouraram Hamed , Reza Dorosti Ahmad , Elmadfa Ibrahim , Abtahi Mitra , Sadeghian Saeid , Jandaghi Jafar

Introduction: Obesity and its related diseases like high blood pressure are the main reason of coronary heart diseases that are the second main cause of mortality in I.R of IRAN. The significant rise of obesity in the last decade resulted in corresponding increase in the prevalence of hypertension.Objective: To detect obesity and hypertension, and the relationship between them among Semnanian people, Iran, 2007Methods: In this cros...

ea0016p574 | Obesity | ECE2008

Development of metabolic syndrome is associated with impaired quality of life: longitudinal data from the North West Adelaide Health Study

Phillips Patrick , Baldock Katherine , Chittleborough Catherine , Taylor Anne

Background and aims: Longitudinal data from the North West Adelaide Health Study (NWAHS) were used to examine the effect of development of metabolic syndrome between Stage 1 (2000–2003) and Stage 2 (2004–2006) on quality of life.Material and methods: The NWAHS is a random, representative sample of people aged 18 years and over living in the north west region of Adelaide. Participants were recruited via telephone interviews to attend the clinic....

ea0016p575 | Obesity | ECE2008

Novel potent and selective non-peptide ligands of ghrelin receptor: characterization of endocrine and extraendocrine actions

Torsello Antonio , Bresciani Elena , Moulin Aline , Tamiazzo Laura , Bulgarelli Ilaria , Caporali Simona , Fehrentz Jean-Alain , Martinez Jean , Perrissoud Daniel , Locatelli Vittorio

Several synthetic peptide and non-peptide ligands of the GHS-R1a have been described that release GH and stimulate food intake. Starting from a triazole scaffold, we have designed and prepared novel small molecules with high binding affinity to the GHS-R1a and we have investigated their effects in vitro on free intracellular calcium levels and in vivo on food intake and GH secretion in the rat. In CHO cells transfected with the GHS-R1a, hexarelin stimulated calcium leve...

ea0016p576 | Obesity | ECE2008

Hypoadiponectinemia but not activation of immune markers is associated with impaired glucose metabolism in morbidly obese patients

Schinner Sven , Kempf Kerstin , Overmann Hubert , Rotthoff Thomas , Schott Matthias , Rose Bettina , Scherbaum Werner A , Herder Christian

Objectives: Obesity is the major risk factor for the development of impaired glucose tolerance (IGT) and type 2 diabetes mellitus (T2DM). In addition, increased circulating levels of cytokines and chemokines and decreased adiponectin levels are associated with IGT and T2DM. However, a large part of morbidly obese patient remain normoglycemic. Therefore, we investigated if this protection can be attributed to a lower grade of inflammation or higher adiponectin levels.<p cla...

ea0016p577 | Obesity | ECE2008

Genetic determination of leptin, insulin and adiponectin levels in the Berlin Fat Mouse Inbred 860 line

Hantschel Claudia , Wagener Asja , Schmitt Armin O , Neuschl Christina , Brockmann Gudrun A

Understanding the complexity of polygenic obesity includes the aspect of the hormonal adaptation during the process of selection for high body weight. Therefore we focussed on the analysis of hormones regulating energy homeostasis, growth and insulin sensitivity in the Berlin Fat Mouse Inbred 860 (BFMI860) line which we use as a model for polygenic obesity. The mouse line had been selected for low protein and high fat content before it was inbred. Under a standard maintenance ...