Searchable abstracts of presentations at key conferences in endocrinology
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10th European Congress of Endocrinology


Protein modifications - proteomics

ea0016s5.1 | Protein modifications - proteomics | ECE2008

Comparative proteomics as a tool in biology: the protein microscope

van der Post Sjoerd , Hakansson Joakim , Enerback Sven , Nilsson Tommy

We use redundant peptide counting as a tool to compare, quantitatively, data from mass spectrometry analyses. When combined with hierarchical clustering, a tool commonly used for micro array analysis, we can group proteins based on their relative abundance across samples. In this way, we recently completed a spatial map of the secretory pathway of rat hepatic cells comprising some 1400 different proteins, each with a defined distribution (Gilchrist, Au, Hiding et al. 20...

ea0016s5.2 | Protein modifications - proteomics | ECE2008

Applying proteomics to thyroid tumour diagnosis

Fuhrer Dagmar

The application of proteomics in clinical thyroid logy is innovative and is a novel tool to decipher Pathophysiologie-inherent changes in protein function, which cannot be comprehensively studied by the more widely applied transcriptome methods.In this talk we will provide an overview on methods of thyroid proteomics and their possible pitfalls, present current studies on this topic and discuss our own experience with the application of proteomics techno...

ea0016s5.3 | Protein modifications - proteomics | ECE2008

Targeting ubiquitin networks

Dikic Ivan

Ubiquitin (Ub) plays a critical role in many fundamental processes like cell cycle, apoptosis, DNA repair or endocytosis. In these processes Ub acts as a signalling component able to trigger molecular events in cells. Ub does so by operating as a reversible and highly versatile regulatory signal for an expanding number of Ub-binding domains (UBD) present in cellular proteins that convey Ub signals into appropriate cellular phenotypes. In addition, it is becoming apparent that ...

ea0016s5.4 | Protein modifications - proteomics | ECE2008

Progesterone receptor responses modified by SUMO in the endometrium

Brosens Jan

Cyclic AMP sensitizes human endometrial stromal cells (HESCs) to progesterone and promotes differentiation into decidual cells, a process indispensable for pregnancy. Our studies have shown that cAMP enhances progesterone responses, at least in part, by attenuating ligand-dependent sumoylation of the progesterone receptor (PR). In fact, decidualization is associated with global hyposumoylation and redistribution of SUMO-1 conjugates into distinct nuclear foci. This altered pat...