ISSN 1470-3947 (print) | ISSN 1479-6848 (online)

Endocrine Abstracts (2008) 16 P509

Interleukin-1sz is a positive regulator of TIARP/STAMP2 in 3T3-L1 adipocytes

Grit Sommer, Susan Kralisch, Sebastian Weise, Lossner Ulrike, Bluher Matthias, Stumvoll Michael & Fasshauer Mathias

University of Leipzig, Department of Internal Medicine III, Leipzig, Germany.

Tumor necrosis factor alpha-induced adipose-related protein (TIARP)/six-transmembrane protein of prostate 2 (STAMP2) has been characterized as an adipocyte-expressed protein which might play a crucial role in metabolic homeostasis and inflammatory pathways. In the current study, the impact of insulin resistance-inducing and proinflammatory interleukin (IL)-1ß on TIARP/STAMP2 gene expression was determined by quantitative real-time reverse transcription-polymerase chain reaction in 3T3-L1 adipocytes. Interestingly, TIARP/STAMP2 mRNA synthesis was significantly stimulated by IL-1ß in a dose-dependent fashion with 2.6-fold induction seen at IL-1ß concentrations as low as 0.02 ng/ml and maximal 9.2-fold upregulation found at 20 ng/ml effector. Furthermore, induction of TIARP/STAMP2 mRNA by IL-1ß was time-dependent with maximal 18.6-fold upregulation detectable after 8 h of IL-1ß treatment. Signaling studies suggested that janus kinase 2, nuclear factor κ B, and p44/42 mitogen-activated protein kinase are involved in IL-1ß-induced TIARP/STAMP2 mRNA expression. Taken together, these results show that TIARP/STAMP2 is highly upregulated in fat cells by IL-1ß and might participate in proinflammatory and insulin resistance-inducing effects of this cytokine.

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