Searchable abstracts of presentations at key conferences in endocrinology
Previous issue | Volume 19 | SFEBES2009 | Next issue

Society for Endocrinology BES 2009

ea0019p1 | Bone | SFEBES2009

Project sunshine - is there a seasonal variation in bone mineral density? a retrospective study

Quinton R , Rawlings D , Hanson P

Objective: To determine whether there is a seasonal variation in bone mineral density (BMD) related to exposure to sunlight.Design: A single-centre retrospective analysis of BMD measurements.Participants: Of 10 971 patients who had undergone bone densitometry in the period 2000–2007. 7513 were female and 3458 male.Results: Anonymised data on BMD of lumbar spine was gathered from existing hospital databas...

ea0019p2 | Bone | SFEBES2009

A ten-year epidemiological study of prevalence and incidence of primary hyperparathyroidism in Tayside, Scotland

Yu N , Donnan PT , Murphy MJ , Leese GP

Objective: The clinical presentation of primary hyperparathyroidism (PHPT) has changed dramatically over the last three decades. Previous epidemiological estimations of PHPT are mainly derived from highly selected populations. This paper systematically evaluates the prevalence and incidence of PHPT in Tayside, Scotland, using a real-world unselected population base.Methods: All Tayside residents with an increased serum calcium concentration (>2.55 mm...

ea0019p3 | Bone | SFEBES2009

Bone parameters and body composition in paediatric renal patients in the year following transplantation

Khanna S , Waller S , King D , Wallace M , Maxwell H , Ahmed SF

Introduction: Children with chronic renal failure may grow poorly and have altered bone metabolism and body composition. Post-renal transplant (Tx), improvement in these parameters may be hindered due to glucocorticoid exposure.Aims & methods: To study changes in these parameters after Tx, anthropometry, bone mineral content (BMC) at total body (TB) and lumber spine (LS), lean mass (LM) and fat mass (FM) were analysed by DXA in 15 children (median ag...

ea0019p4 | Bone | SFEBES2009

Effects of five gut hormones on osteoblastic-like cell lines

Pacheco-Pantoja EL , Ranganath L , Wilson P , Gallagher J , Fraser WD

Gut hormones are gastro-entero-pancreatic hormones released during the normal physiological response to feeding/fasting. Adequate nutrient intake and normal gastrointestinal function are critical to bone health, which can be under constant repair and remodelling. Gut hormones may integrate a connection between food intake and bone turnover.We studied the effects of five gut hormones, glucose-dependent insulinotropic polypeptide (GIP), glucagon-like pepti...

ea0019p5 | Bone | SFEBES2009

Identification of a kindred from Northern Ireland with familial hypocalciuric hypercalcaemia type 3, which maps to chromosome 19q13.3

Nesbit MA , Hannan FH , Graham U , Hunter S , Morrison PJ , Thakker RV

Familial hypocalciuric hypercalcaemia (FHH) is an autosomal dominant disorder of mineral metabolism that is characterized by lifelong elevation of serum calcium concentrations associated with inappropriately low urinary calcium excretion (calcium clearance:creatinine clearance <0.01). Three separate FHH loci have been identified (FHH1-3). Loss-of-function mutations of the calcium-sensing receptor (CaSR) gene located on 3q21.1, which account for the majority of FHH c...

ea0019p6 | Bone | SFEBES2009

A Ser1386Pro mutation in the C-propeptide domain of Col2a1 results in spondyloepiphyseal dysplasia congenita in mice

Esapa C , Hough T , Testori S , Head R , Crane E , Chan C , McNally E , Carr A , Brown M , Croucher P , Nesbit M , Brown S , Cox R , Cheeseman M , Thakker R

We are investigating mice treated with the chemical mutagen N-ethyl-N-nitrosourea (ENU) to establish models for hereditary bone disorders such as osteoporosis, osteoarthritis and skeletal dysplasias. We report on one such mouse model designated Longpockets (Lpk). Mice were kept in accordance with national welfare guidelines and project license restrictions. Lpk/+ mice are viable and fertile, present with short humeri by two weeks of birth and have a...

ea0019p7 | Bone | SFEBES2009

A mouse model, Hcalc1, for autosomal dominant hypercalciuria is due to a transient receptor potential cation channel, subfamily V, member 5 (Trpv5) mutation

Loh N , Stechman M , Ahmad B , Hannan F , Hough T , Chiev K-P , Stewart M , Bentley L , Cox R , Brown S , Thakker R

To identify genes causing hypercalciuria, we screened male offspring of C57BL/6J male mice mutagenised by N-ethyl-N-nitrosourea (ENU) for this abnormality. Mice were kept in accordance with UK Home Office welfare guidelines and project licence restrictions. Metabolic cage studies were performed to collect 24-hour urine samples, and this revealed one mouse with hypercalciuria (Hcalc1). Inheritance testing demonstrated that Hcalc1 was inherited as an autosomal domi...

ea0019p8 | Bone | SFEBES2009

The potential inhibitory role of SOCS2 in chondrocyte GH/IGF-1 signalling during chronic inflammatory diseases

Pass C , MacRae V , Ahmed S , Farquharson C

The actions of SOCS2 on GH and IGF-1 signalling in growth plate chondrocytes have yet to be reported. During chronic paediatric inflammatory diseases retarded growth is seen in association with increased inflammatory cytokine levels, which is an effect that may involve SOCS2. The primary aim of this study was to investigate STAT signalling in chondrocytes in response to GH, IGF-1 and IL-1β. We also investigated the temporal expression of SOCS2 in response to GH and IGF-1 ...

ea0019p9 | Bone | SFEBES2009

Effects of adiponectin on the osteosarcoma cell line Saos-2

Pacheco-Pantoja EL , Sodi R , Gallagher J , Fraser WD

Adiponectin, the most abundant peptide hormone secreted from adipose tissue, has been negatively correlated with obesity and can induce varying responses on bone. There are significant discrepancies in the published data, showing increased, decreased or no effects on bone turnover.We investigated the effects of adiponectin on the human osteosarcoma cell line Saos-2. After culture with varying concentrations of adiponectin (10–100 nM), supernatants w...

ea0019p10 | Bone | SFEBES2009

Bone mineral density and body composition in adult men and women with CAH hyperplasia: congenital adrenal hyperplasia adult study executive

Han T , Willis D , Wild S , Ross R , Conway G , CAH Adult Study Executive CaHASE

Objectives: To assess bone mineral density (BMD) and body composition in adults with congenital adrenal hyperplasia (CAH).Setting: Endocrine clinics in seventeen centres throughout the UK participating in CaHASE, a Society for Endocrinology project.Study design and subjects: Cross-sectional survey of 203 adults with CAH (65 men, 138 women: median age 33 (range 18–70) years). Hundred and sixty six patients were classified as cl...

ea0019p11 | Bone | SFEBES2009

Impact of body mass index at baseline on parathyroid hormone and phospho-calcium metabolism in adult growth hormone deficient patients before and after growth hormone replacement therapy

Mon Aung , Ahmad Aftab , Malipatil Nagaraj , Joseph Franklin , White Helen , Joshi Ashwin , Sharma Dushyant , Fraser William , Vora Jiten

Background: Adult growth hormone deficient (AGHD) is associated with reduced bone turnover and reduced bone mineral density (BMD): factors improved by growth hormone replacement (GHR). Obesity is associated with reduced bone turnover but increased BMD. We investigated the impact of body mass index on parathyroid hormone (PTH), phospho-calcium metabolism and bone turnover in AGHD patients before and after GHR.Study design: Of 31 AGHD patients (15 obese pa...

ea0019p12 | Bone | SFEBES2009

Mesenchymal stem cell differentiation to osteoblasts and adipocytes is associated with differential adenosine receptor expression

Gharibi Borzo , Ham Jack , Evans Bronwen

Osteoblasts and adipocytes are derived from mesenchymal stem cells (MSC), although the mechanisms involved are not fully understood. Our previous findings showed that MSCs express functional adenosine receptors (ARs) and that adenosine stimulates osteoblastogenesis. Here we describe changes in AR expression as MSCs differentiate into osteoblasts and adipocytes, and provide evidence that ARs are involved in these differentiation pathways.Rat MSCs were dif...

ea0019p13 | Bone | SFEBES2009

Hyperparathyroidism secondary to a parathyroid carcinoma

Chikkaveerappa K , Jones IA , Wynne S , Leong KS

We present a rare case of hypercalcaemia caused by a parathyroid carcinoma.An asymptomatic 74 year old gentleman was referred with slightly raised alkaline phosphatase and corrected serum calcium the results of which are shown below:Serum Calcium 3.02 mmol/l, Albumin 41 g/l, PTH 18.7 pmol/l, Urine Calcium 10.6 mmol/24 h, TSH 6.3 Mu/l, FT4 12.3 pmol/l, FT3 5.1 pmol/l, TPO antibody >5000 IU/ml, ALP 142 iu/l.<p class="abstext"...

ea0019p14 | Bone | SFEBES2009

Osteoporosis as a major risk for patients with glycogen storage disease

Geberhiwot Tarekegn , Cooper Mark , Ayuk John , Toogood Andrew , Newsome Philip , Gittoes Neil

Glycogen storage diseases (GSD) are autosomal recessive inborn errors of carbohydrate metabolism. With current dietary therapy, life expectancy in patients with GSD has improved considerably and almost all children reach adulthood. Notwithstanding intensive therapy, patients with GSD have an increased risk of osteoporosis. We followed 20 patients aged 22–62 (mean age of 37) years with GSD type I, III and IX, for up to 5 years with serial measurements of bone turnover mark...

ea0019p15 | Bone | SFEBES2009

Novel mutation of the COL1A2 gene in a patient with osteogenesis imperfecta

Pua S , Ashawesh K , Murthy NPN , Randeva H

Introduction: Osteogenesis imperfecta (OI) is classified on the basis of molecular genetics, mode of inheritance, clinical and radiological findings. The genes implicated are either COL1A1 or COL1A2. We report a case of a novel mutation of OI in the COL1A2 gene.Case: A 24-year-old teacher with type 1 Diabetes Mellitus, presented to our Endocrine Clinic with recurrent fractures since childhood. At 7 months old, she fractured her left leg. At...

ea0019p16 | Bone | SFEBES2009

Familial hypocalciuric hypercalcaemia and pregnancy outcome

Murthy A , Murthy NPN , Ashawesh K , Kulambil Padinjakara RN , Anwar A

Introduction: Hypercalcaemia during pregnancy poses a risk to both the mother and foetus and can present a complex management issue. Although hypercalcaemia from any cause can occur during pregnancy, primary hyperparathyroidism is the most common cause but other rarer cause such as familial hypocalciuric hypercalcaemia (FHH) should be ruled out. Although usually posing no risk to the mother, infants of mothers with the disease are at risk for neonatal hypocalcaemia, and seizur...

ea0019p17 | Bone | SFEBES2009

Determinants of vitamin D deficiency in chronic pancreatitis

Selby P , Prasad N , Makin A , Berry J

Chronic pancreatitis (CP) may lead to deficiency of fat soluble vitamins such as vitamin D; little is known of the extent of this in chronic pancreatitics.In order to assess the extent and severity of vitamin D deficiency in CP we examined the notes of 111 patients with CP. In addition to clinical information and calcium, vitamin D and PTH levels, exocrine function was evaluated using the PABA test to obtain a pancreatic excretion index (PEI: normal &#62...

ea0019p18 | Bone | SFEBES2009

Renal bone disease control: comparison between diabetics and non diabetics in 187 haemodialysis patients

Panteliou E

Objectives: To compare the diabetic (DM) (n=124) and non diabetic (nonDM) (n=63) patients on haemodialysis (HD) in terms of renal bone disease (RBD) control.Methods: Retrospective case-note review of 187 HD patients. The National Kidney Foundation guidelines for chronic kidney disease stage 5 (CKD-5) were used, with target levels for parathormone (PTH) 16.5–33 pmol/l, calcium 2.1–2.35 mmols/l and phosphate 1.78 mmols/l.<p clas...

ea0019p19 | Bone | SFEBES2009

Primary hyperparathyroidism: osteopaenia and osteoporosis

Ajala O , Thondam S , Adams JE , Khaleeli A

Objective: To determine the incidence of osteopaenia and osteoporosis in primary hyperparathyroidism.Methods: Retrospective review of 80 patients with primary hyperparathyroidism; Bone densitometry of the femoral neck, lumbar spine and distal ulna was assessed by dual energy X-ray absorptiometry (DEXA) at the time of diagnosis.Results: The median age at diagnosis was 68; The male: female ratio was 1:3; osteoporosis was present in 2...

ea0019p20 | Bone | SFEBES2009

A case of severe hyperparathyroidism with brown tumour and vitamin D deficiency- treated as simple bone cyst

Suresh Damodharan , Nethaji Chithambaram , Hyatt Penny , Halliday Neil

Brown tumour is a fibrous tissue mass containing hemosiderin-pigmented macrophages/ multinucleated giant cells, replacing/expanding part of bone in primary hyperparathyroidism. We present a case with brown tumour who was treated as simple bone cyst.A 22-year-old lady presented to rheumatology with pain in arms and legs. Investigations revealed severe vitamin D/B12/folate/iron deficiency with positive IgA. Gliadin antibodies and duodenal biopsy confirmed ...

ea0019p21 | Bone | SFEBES2009

Activation of AMP-activated protein kinase represses chondrocyte proliferation

Dobson Pauline , Collins John , El-Mastry Omar , Goldring Mary , Brown Barry

AMP-activated protein kinase (AMPK) acts as a sensor and regulator of cellular energy homeostasis. Once activated, AMPK influences a number of responses including inhibition of anabolic pathways and the switching-on of catabolic pathways. Regulation of AMPK, which responds to an increase in the AMP/ATP ratio, could have an important role in chondrocyte function particularly in response to hormones and to stress such as occurs in osteoarthritis. Therefore, we sought to investig...