Searchable abstracts of presentations at key conferences in endocrinology
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11th European Congress of Endocrinology

Poster Presentations


ea0020p618 | Reproduction | ECE2009

The effect of nutritional supplement (Menopace®) on the frequency of hot flushes, night sweats, mood and quality of life in post-menopausal women: a placebo-controlled double blind study

Andrikoula Maria , Baker Dawn , Nesic Jelena , Liao Lih Mei , Duka Theodora , Prelevic Gordana

Objective: Hot flushes and night sweats experienced by 60–70% of postmenopausal women are considered as classical signs of menopause. Estrogens is the gold standard treatment, but in view of its potential risks, various herbal preparations and vitamin supplements have a great appeal to women. Aim of this study was to assess the effect of a nutritional supplement (Menopace®) on the frequency and severity of hot flushes and the quality of life in postmenopausal women i...

ea0020p619 | Reproduction | ECE2009

Clinical characteristics of Algerian PCOS women

Samia Ould-Kablia , Yamina Aribi , Zahra Kemali , Mourad Semrouni

Polycystic ovary syndrome (PCOS) is the most frequent endocrinopathy in premenopausal women, and has many clinical features in common with the metabolic syndrome.Objective: The aim of our study was to evaluate the clinical characteristics of our PCOS women.Methods: Of 181 PCOS defined by the Rotterdam criteria and 90 controls were evaluated for anthropometric parameters by physical examination.Results: The PC...

ea0020p620 | Reproduction | ECE2009

Influence of hyperprolactinemia on prolactin receptor manifestation and hepatic bilirubin excretory activity under condition of female rat obstructive cholestasis

Kushnareva Natalia , Sergeeva Maria , Smirnova Olga

Ordinary complication of pregnancy is shift of hepatic excretory activity leading to cholestasis. Gallstone disease is also predominated in women. Both high prolactin (Prl) concentration and high level of liver prolactin receptors (PrlR) in normal and pregnant women were assumed to participate in obstructive cholestasis development.Using female rat model of hyperprolactinemia combined with obstructive cholestasis we aimed to investigate Prl influence on ...

ea0020p621 | Reproduction | ECE2009

The prevalence of subclinical late – onset hypogonadism in men with diabetes type 2

Vasilkova Olga , Mokhort Tatyana , Sharshakova Tamara , Sanec Igor

The aim: To investigate the prevalence of subclinical late-onset hypogonadism (SLOH) in men with diabetes type 2 and relationship between testosterone concentrations and duration of diabetes.Material and methods: We investigated 114 men with DT 2 aged from 45–60 years (M±SD 54.0±4.63). Luteinizing hormone (LH), follicle-stimulating hormone (FSH), total testosterone (T), calculated free testosterone (cFT) and bioavailable testosterone (bT),...

ea0020p622 | Reproduction | ECE2009

Adiponectin increases insulin-like growth factor I-induced progesterone and estradiol secretion in human granulosa cells

Chabrolle Christine , Tosca Lucie , Rame Christelle , Lecomte Pierre , Royere Dominique , Dupont Joelle

Adiponectin is a protein hormone mainly produced by adipocytes. It plays an essential role in the regulation of lipid and carbohydrate metabolisms. Adiponectin mediates its effects through mainly two receptors named AdipoR1 and AdipoR2. Some evidence in rodent and domestic animals suggests that adiponectin could also regulate female fertility and more particularly ovarian functions. However, its role in human ovary has never been investigated. The objectives of the present stu...

ea0020p623 | Reproduction | ECE2009

Effects of thyroid hormones on ovary granulosa cells: regulation of proliferation, survival and function

Misiti Silvia , Falzacappa Cecilia Verga , Mangialardo Claudia , Bucci Barbara , Raffa Salvatore , Silvestrini Giuliana , Stigliano Antonio , Toscano Vincenzo

It is clinically evident that women suffering from thyroid disorders are associated with frequent occurrence of menstrual disturbances and impaired fertility, and these abnormalities are improved by restoring the euthyroid state. The exact mechanism for such reproductive aberrations is not well known; however, it is conceivable that thyroid hormones might have a direct role in ovarian physiology via receptors in granulosa cells. We evaluated the effect of thyroid hormones (T<s...

ea0020p624 | Reproduction | ECE2009

Successive degradation of StAR by soluble and membrane-bound mitochondrial proteases: plausible mechanism protecting steroidogenic mitochondria under ‘protein stress’

Bahat Assaf , Orly Joseph

Steroidogenic acute regulatory protein (StAR) is a nuclear encoded vital mitochondrial protein that is essential for synthesis of steroid hormones in the adrenal and gonads. In these tissues, StAR mediates translocation of cholesterol into the inner mitochondrial membranes, where it is converted to the first steroid, pregnenolone. The roughly understood mechanism of StAR action is probably executed prior to StAR import, when the protein is paused at the surface of the outer mi...

ea0020p625 | Reproduction | ECE2009

AdipoR1 and AdipoR2 Inactivation by RNA interference in the KGN human granulosa cell line: potential involvement of AdipoR1 in cell survival and AdipoR2 in steroidogenesis

Peggy Pierre , Pascal Froment , Didier Negre , Christelle Rame , Christine Chabrolle , Pierre Lecomte , Joelle Dupont

Adiponectin is one of the most abundant fat-derived hormones involved in a multitude of metabolism pathways. It acts as an anti-diabetic and anti-atherogenic adipokine. Adiponectin mediates its actions through mainly two receptors, AdipoR1 and AdipoR2. It has been postulated that although AdipoR1 and AdipoR2 consist of seven transmembrane helices, they are distinct from other G protein-coupled receptors. A role of adiponectin in ovarian physiology has been recently suggested. ...

ea0020p626 | Reproduction | ECE2009

Progesterone suppression of the male hypothalamo-pituitary gonadal axis is partially reversed by the progesterone antagonist mifepristone

McIntosh Joseph , Walley Ros , Wittke Baerbel , Sudworth David , Howe David

Background: Daily injection of progesterone in men is reported to suppress the pituitary LH response to GnRH challenge. We hypothesized that the progesterone nuclear hormone receptor antagonist mifepristone (RU486) would reverse these effects.Study design: Open–label randomised, three period crossover-over study in 12 healthy male subjects. Subjects were treated for 8 days with A/ progesterone 50 mg (IM) alone; B/ progesterone 50 mg + mifepristone 1...

ea0020p627 | Reproduction | ECE2009

Isradipine inihibits oxytocin-induced contractions of isolated myometrium from late pregnant rat

Nalbant Mehmet , Kutlu Selim , Kumru Selahattin , Ozcan Mete , Alcin Ergul , Gurates Bilgin , Ayar Ahmet

Preterm labour is a serious clinical problem in obstetrics, and affective treatment of this condition still far from satisfactory. The purpose of this in vitro study was to examine the effects of isradipine, a calcium channel antagonist, on oxytocin-induced contractions of myometrium. Myometral strips were removed from late pregnant (18th day) Wistar rats following decapitation and placed in a jacked tissue bath containing Krebs’ solution and isometric contractions...

ea0020p628 | Reproduction | ECE2009

Obesity resulting from Gs-alpha mutations in the maternal (but not paternal) allele is a consequence of Gs-alpha imprinting in the central nervous system

Esponda Pedro , Relloso Miguel

The female genital tract of the mouse was in vivo transfected using the reporter gene β-galactosidase and the liposome Lipofectamine as gene vector. All animals used were anaesthetised. DNA/Liposome complexes were injected through the infundibulum of the tubes in adult, immature and pseudopregnant females. Females that were at different stages of the ovarian cycle were also used. Transfection was analyzed using histochemical, immunological and molecular (Southern b...

ea0020p629 | Reproduction | ECE2009

Prolonged qt (corrected) dispersion in women with polycystic ovary syndrome

Sezer Kerem , Pata Ozlem , Camsari Ahmet

Background: Polycystic ovary syndrome is the most common endocrinopathy among females in reproductive ages. PCOS is not accepted only a reproductive pathology but also accepted as a metabolic problem. Prolonged QTc interval showing ventricular repolarization is a major risk factor for the arrhythmias, coronary heart diseases, and sudden cardiac death. Although hyperinsulinism and hyperandrogenism is known as common manifestations of PCOS, there is conflicting data a...

ea0020p630 | Reproduction | ECE2009

Free thiols in human spermatozoa: correlation with Na+/K+-ATPase, Ca2+-ATPase activities and sperm motility

Vignini Arianna , Buldreghini Eddi , Nanetti Laura , Raffaelli Francesca , Paggi Francesca , Boscaro Marco , Mazzanti Laura , Balercia Giancarlo

The aim of the present study was to measure free thiols content, Na+/K+-ATPase, Ca2+-ATPase activities in human spermatozoa of asthenozoospermic patients and normospermic donors, and evaluate any influence on kinetic sperm features, as well as correlation with peroxynitrite. In fact, membrane integrity and its composition are the basic characteristics of the sperm membrane, thus, it is evident that its composition is an important factor for mem...

ea0020p631 | Reproduction | ECE2009

Influence of polycystic ovary syndrome and obesity on vascular parameters (common carotid artery intima-media thickness – CCA-IMT, common carotid artery compliance – CP-CCA and endothelium function of the brachial artery – FMD) related to the process of atherosclerosis

Marcondes Jose , Barcellos Cristiano , Lage Sylvia , Romano Angela , Rocha Michele , Hayashida Sylvia , Curi Daniela , Baracat Edmund

In order to determine the influence of PCOS and obesity on vascular parameters, we studied 25 patients with PCOS (10 with normal body mass index – BMI – and 15 obese) without classic cardiovascular risk factors (IGT or DM, arterial hypertension, dyslipidemia) and 23 control women (12 with normal BMI and 11 obese), pairwise matched for BMI, through a non-invasive method using high resolution ultrasound imaging. Global age range was 26.0±4.7 years. The mean values...

ea0020p632 | Reproduction | ECE2009

Impact of the type of diabetes in pregnancy upon pregnancy outcome

Elazrag Malak , Sultan Mohammed , Shaliry Hamida

Background and aims: It is well known that most of the pregnancy complications (maternal, fetal, & neonatal) increased noticeably in diabetic pregnancy compared with non diabetics, our aims of this study is the impact of the type of diabetes in pregnancy upon pregnancy outcome.Methods: This is a crosse study analyzed 210 pregnant women who are undergoing antenatal follow up in diabetic pregnancy clinic, and then delivered in Aljalaa Maternity Hospita...

ea0020p633 | Reproduction | ECE2009

Advanced glycosylated end products are associated with anovulatory markers in women with polycystic ovary syndrome

Diamanti-Kandarakis Evanthia , Piouka Athanasia , Livadas Sarantis , Piperi Christine , Katsikis Ilias , Papavasiliou Athanasios , Panidis Dimitrios

Background: Chronic anovulation constitutes one of the cardinal features of Polycystic Ovary Syndrome (PCOS) and Anti-Müllerian hormone (AMH), a granulosa cell product, is a well documented marker of anovulation. Recent data have shown that oxidative stress is involved in the pathophysiology of anovulation. A marker of oxidative stress, Advanced Glycosylated End products (AGEs), is implicated to the pathogenesis of the syndrome and have been localized with high intensity ...

ea0020p634 | Reproduction | ECE2009

A systematic review and a meta-analysis on adiponectin levels in women with polycystic ovary syndrome

Toulis Konstantinos , Goulis Dimitrios , Farmakiotis Dimitrios , Katsikis Ilias , Georgopoulos Neoklis , Tarlatzis Basil , Papadimas Ioannis , Panidis Dimitrios

Background: Conflicting results regarding adiponectin levels in women with polycystic ovary syndrome (PCOS) have been reported. To evaluate adiponectin levels in PCOS, a systematic review of all studies comparing adiponectin levels in women with PCOS to healthy controls and a meta-analysis of those involving women with similar Body Mass Index (BMI) were performed. Influence of possible effect modifiers, such as insulin resistance (IR) and testosterone, was investigated. Influe...

ea0020p635 | Reproduction | ECE2009

Gonadotropin releasing hormone-induced histone modifications and gonadotropin gene expression

Melamed Philippa , Wijeweera Andrea

Gonadotropin releasing hormone actively de-represses expression of the gonadotropin subunit genes through several actions that target the chromatin. We have shown in the past that both LHβ and FSHβ genes are repressed by histone deacetylases (HDACs) in αT3-1 gonadotrope cells and that this repression is overcome by exposure to GnRH which facilitates HDAC removal via CaMKI-mediated phosphorylation. At the LHβ gene promoter, both histone acetylation and phosp...

ea0020p636 | Reproduction | ECE2009

Candidate gene analyses in Caucasian patients with primary ovarian insufficiency

Rossetti Raffaella , Cacciatori Chiara , Marozzi Anna , Cordella Daniela , Bione Silvia , Cannavo Salvatore , Bernard Dan , Cole Trevor , Clayton-Smith Jill , Beck-Peccoz Paolo , Persani Luca

Primary ovarian insufficiency (POI) is a heterogeneous disorder characterized by primary (PA) or secondary (SA) amenorrhea associated with increased levels of gonadotropins. POI affects about 1% of women before the age of 40 years. A major genetic component has been suggested for idiopathic POI due to the frequent familiarity for this defect. Indeed, FMR1 premutations can be found in 10–15% and BMP15 mutations in 2–5% of POI patients. Numerous other candidate genes h...

ea0020p637 | Reproduction | ECE2009

Penile size and testicular volume in healthy Lithuanian newborns

Preiksa Romualdas Tomas , Zilaitiene Birute , Matulevicius Valentinas

Aim: Of the study was to establish normal penile size and testicular volume in healthy Lithuanian newborns.Methods: Of 1204 newborn boys were examined in Panevėžys Hospital due to genitometry. Those with cryptorchidism or hypospadias were excluded. Penis size was measured in 1042 and testicular volume in 712 healthy newborns. The stretched penile length was measured between the pubic ramus and the tip of the glans and the diameter – in...

ea0020p638 | Reproduction | ECE2009

The effects of stress on the histological structure of rat testis

Demirci Tuba , Ozbek Elvan

Objective: Unexplained infertility is 15% among all infertility cases. Many forms of stress, including psychological and physical can effect male fertility and reproductive activity. In this study, we aimed to investigate possible histopathological effects of physical and psychological stress on rat testis at light microscopic level.Methods: Eight adult, male Sprague Dawley rats were used in this study. Rats were randomly divided as control (n=4) ...

ea0020p639 | Reproduction | ECE2009

Effect of lamotrigine and carbamazepine on selected reproductive hormones, lipid profiles and ovarian histology in female rats

Mohammad Bassim Irheim , Al-Mousawi Najah , Al-Terahi Azhar , Hassan Ekhas

Objectives: This study was conducted to evaluate the effect of lamotrigine (LTG) & carbamazepine (CBZ) on reproductive hormones (follicle-stimulating hormone (FSH), luteinizing hormone (LH) & total testosterone), lipid profiles (total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL), low density lipoprotein (LDL) & very low density lipoprotein (VLDL)), ovarian weight & histology in non epileptic female rats.Materials an...

ea0020p640 | Reproduction | ECE2009

Effects of electromagnetic fields of cellular phone on testosterone and progesterone hormones rate in Syrian Hamsters (Mesocricetus auratus)

Lotfi Alireza , Aghdam Shahryar Habib

In this study, the effects of exposure to a 900 MHz electromagnetic field (EMF) emitted from cellular phones on serum testosterone and progesterone hormones rate of adult male Syrian Hamster were evaluated. 72 male Hamster divided in 3 groups include: 1) control group, without any radiation, 2) exposed for 10 days and under 900 MHz EMF (emitted from cellular phone) for 1 h daily, 3) exposed for 50 days and under 900 MHz EMF for 1 h daily. In end of experiment, blood samples co...

ea0020p641 | Reproduction | ECE2009

Is there a need for withdrawal test with oral micronized natural progesterone in the evaluation of women with polycystic ovary syndrome?

Livadas Sarantis , Boutzios Georgios , Economou Frangiskos , Alexandraki Krystallenia , Xyrafis Xenofon , Zerva Aristea , Karachalios Athanasios , Diamanti-Kandarakis Evanthia

Objective: To investigate the effects of oral natural micronized progesterone on hormonal and metabolic parameters in patients with polycystic ovary syndrome (PCOS) and to answer the clinical question if induction of withdrawal bleeding is a necessity for the comparison of hormonal and metabolic data in PCOS subjects.Design: Prospective clinical study.Setting: Academic medical centre.Population: Twenty eight ...

ea0020p642 | Reproduction | ECE2009

Gonadal function in male mountain bikers

Yamaner Faruk , Atmaca Hulusi , Bayraktaroglu Taner , Aydin Mustafa , Aydemir Selim

Objectives: Mountain biking has become increasingly popular worldwide. Mountain bikes, are a common source of significant injuries including chronic overuse injuries affecting the genitourinary tract. Erectile dysfunction and infertility are some of the reported urogenital problems in male riders. Our aim was to evaluate the testicular function in male mountain bikers.Methods: Thirty male professional mountain bikers with a mean age of 23.4±2.5 year...

ea0020p643 | Reproduction | ECE2009

The effects of metformin therapy in women with idiopathic hirsutism

Unluhizarci Kursad , Ahu Baysal Nuran , Karaca Zuleyha , Tanriverdi Fatih , Kelestimur Fahrettin

Hirsutism, which is characterized by excessive growth of terminal hair in a male pattern, is a common clinical condition in women. Among its etiologies, idiopathic hirsutism (IH) is considered to be one of the most common forms of hirsutism. We have previously shown the presence of insulin resistance in women with IH and our aim was to investigate the effects of metformin therapy in women with IH. The study was approved by the local Ethics Committee. Sixteen women with IH (mea...

ea0020p644 | Reproduction | ECE2009

Impact of gestational impaired glucose tolerance test on pregnancy outcome

Sultan Mohamed , Elazrag Malak , Khiaif Huda

Background and aims: The high prevalence of diabetes and IGTT in women of childbearing age in our community provides an opportunity to assess prospectively the risks of adverse outcome in pregnancy of these conditions.Method: This retrospective analysis data of a 114 pregnant women diagnosed to have gestational impaired glucose tolerance test, in the period from beginning of January 2001 to the end of December 2004 from maternal & neonatal documents,...

ea0020p645 | Reproduction | ECE2009

A monocentric study of 360 consecutive patients presenting with premature ovarian failure

Bachelot Anne , Rouxel Agnes , Massin Nathalie , Dulon Jerome , Courtillot Carine , Kuttenn Frederique , Touraine Philippe

Premature ovarian failure (POF) encompasses a heterogeneous spectrum of conditions, with phenotypic variability among patients. The etiology of POF remains unknown in most cases. This led us to set up a French network on POF for the purpose of better characterizing POF patients and understanding the mechanisms involved in this pathology. Over the last 10 years, we have evaluated 360 women who were referred to our center with a diagnosis of POF, and performed a study of clinica...

ea0020p646 | Reproduction | ECE2009

Effect of an oral contraceptive on emotional distress of women with polycystic ovary syndrome: a prospective study

Cinar Nese , Harmanci Ayla , Demir Basaran , Yildiz Bulent O

Background: The symptoms typically associated with polycystic ovary syndrome (PCOS) such as acne, hirsutism, irregular menses, amenorrhoea, obesity and infertility are a major source of psychological morbidity and can negatively affect quality of life (QOL). Limited data are available regarding effects of different treatment modalities on emotional distress of women with PCOS.Objective: To determine potential impact of treatment on health-related quality...

ea0020p647 | Reproduction | ECE2009

Spermatogenesis in men with late-onset hypogonadism, receiving testosterone-gel

Mskhalaya George , Tishova Yulia , Vadov Vadim , Gusakova Dariya , Kalinchenko Svetlana

The prevalence of LOH among middle-aged men, who want to have kids, is increasing. According to HIM study (Mulligan T, Frick MF 2006), the prevalence of LOH is 34% in men, aged 45–54. There are 3 wide-spread forms of testosterone treatment of LOH: oral, i/m forms, and gels. Oral forms of treatment are known to have a rather weak effect. I/m forms of testosterone treatment have a significant negative effect on spermatogenesis down to azoospermia, causing decrease in LH and...

ea0020p648 | Reproduction | ECE2009

Prostatic secreted proteins in mice and rats: Identification using mass spectrometric analysis and the hormone dependent expression

Fujimoto Nariaki , Suzuki Tomoharu , Ohta Shigeru

It is not surprising that mouse prostate gland is anatomically very similar to that in the rat, which consists of the ventral prostate (VP), dorsolateral prostate (DLP) and anterior prostate (AP). However, the basic biological function of the prostate, prostatic secretion, has been suggested to be diverse between the two species and yet poorly understood. We have identified major secreted proteins from separate prostate lobes of the mouse as well as the rat by mass spectrometr...

ea0020p649 | Reproduction | ECE2009

Erectile dysfunction among HIV-infected men is associated with protease inhibitor containing HAART

Moreno-Perez Oscar , Escoin Corina , Serna-Candel Carmen , Arias Nieves , Alfayate Rocio , Boix Vicente , Merino Esperanza , Portilla Joaquin , Pico Antonio

Background: Erectile dysfunction (ED) is an increasingly recognized condition among HIV-infected, however its prevalence and etiology remain unclear. Our aim was to asses prevalence and risk factors associated to ED in this population.Methods: A cross-sectional study was performed, 88 male HIV-positive without hepatitis C or diabetes were studied. Patients were classified according antiretroviral treatment (ART): naïve, protease inhibitor (PI)-conta...

ea0020p650 | Reproduction | ECE2009

Abstract withdrawn....

ea0020p651 | Reproduction | ECE2009

Screening of maternal thyreopathies in Slovakia: is it worth it?

Urbankova Helena , Hruska Jan , Vanuga Peter

Background: Newborn screening is the only systematic work-up of thyroid disorders in Slovakia with unambigously proven positivities. There is wide discussion regarding the screening of thyreopathies in specific risk groups such as pregnant women.Aims: To study the gynaecologist and endocrinologist co-operation in screening pregnant patients from region of Liptov and Orava for autoimmune thyroiditis.Subjects and methods: The partici...

ea0020p652 | Reproduction | ECE2009

Temporal dexamethasone treatment: responses in uterine proliferative mechanisms induced by neurogenic stimulation

Spencer Fitzgerald , Qi Limen

Mechanical vagino-cervical stimulation (vcs) or mating stimulates hypothalamic neurons that regulate pulsatile secretion of gonadotropin-releasing hormone and the gonadotropins which affect decidualization, an essential for successful mammalian pregnancy, via induced ovarian steroidogenesis. Progesterone-regulated deciduomal growth is promoted by paracrine factors plus the uterine matrix metalloproteinases (MMPs) that remodel the decidual tissue, and nitric oxide (NO), a regul...

ea0020p653 | Reproduction | ECE2009

Hyperprolactinemia in polycystIc ovary syndrome

Agbaht Kemal , Yerlikaya Halis , Demir Ozgur , Gullu Sevim

Background: Hyperprolactinemia is frequently reported with polycystic ovary syndrome (PCOS). However, there is a controversy whether they share a common mechanism or have cause–result relationship or just are coincidental. The objective of the present study was to identify the cause of hyperprolactinemia in patients with PCOS.Methods: We retrospectively evaluated our outpatient admission records for PCOS, hirsutism, oligomenorrhea, or secondary amen...

ea0020p654 | Reproduction | ECE2009

Serum androgen levels correlate well with metabolic parameters in polycystic ovary syndrome

Agbaht Kemal , Yerlikaya Halis , Gullu Sevim

Background: In polycystic ovary syndrome (PCOS), in response to stimulation by luteinizing hormone (LH), the ovarian theca cell synthesizes androgens. However, associations of serum androgen levels with metabolic parameters yet to be determined. We aimed to investigate these associations.Methods: We retrospectively evaluated our outpatient admission records for PCOS, hirsutism, oligomenorrhea, or secondary amenorrhea. PCOS diagnosis was made after exclud...

ea0020p655 | Reproduction | ECE2009


Vilela Mrcio , Lucio dos Santos Juniora Joao , Ziyona Mamman

Objective: In order to determine the plasmatic levels of leptin in adult Wistar rats (90 days +7 days) which had undergone specific types of stress, a prospective study was done in which the stimulus was presented in the proestrus stage and the samples, recovered by decapitation 24 h after the initial stimulus, were centrifuged and stored for analysis of leptin by radioimmunoassay (RIA).Methods: The rats were divided into four groups: Group I (...

ea0020p656 | Reproduction | ECE2009

Use of estradiol benzoate in presynch method in lactating dairy cows

Laleh Vahid Ghaffari , Asadi Ali , Mohammadi Mohammad Tagi

The aim of this study was to determine of pregnancy rate when Estradiol Benzoate (EB) used in presynch method than to routine presynchronization protocol in lactating dairy cows (N: 210). In this study, cows divided in two treatment groups: control group (N: 100) and main group (N: 110). Control group cows received two injections of Cloprostenol (750 μg/Case, IM) with 14 d interval. Then, they received an injection of Gonadorelin (100 μg/Case, IM...

ea0020p657 | Reproduction | ECE2009

Prolonged treatment with N-acetylcysteine and L-arginine restores gonadal function in patients with PCO syndrome

Masha Andi , Manieri Chiara , Dinatale Stefano , Bruno Guido Albino , Ghigo Ezio , Martina Valentino

Introduction: Nitric oxide (NO) plays a wide spectrum of biological actions including a positive role in oocyte maturation and ovulation. Free radicals have been shown elevated in PCOS and therefore would be responsible for quenching NO that, in turn, would play a role in determining oligo or amenorrhea connoting PCOS. We recently demonstrated that the combined administration of N-acetylcysteine (NAC) and arginine (ARG) is able to exert an antioxidant action leading to ...

ea0020p658 | Reproduction | ECE2009

Does the point of time of menstrual irregularities appearance influence clinical, metabolic, hormonal profile and ultrasound findings in PCOS women?

Economou Frangiskos , Livadas Sarantis , Christou Maria , Christakou Charikleia , Palymeri Elvina , Palioura Eleni , Tantalaki Evangelia , Diamanti-Kandarakis Evanthia

Background: PCOS is characterized from chronic anovulation, clinically expressed as menstrual irregularities, hyperandrogenism and polycystic ovaries on ultrasound. A growing body of data indicates that PCOS is a life existing disease starting before puberty and lasting post menopause, as hormonal and metabolic changes exist through the life span of the patient. Since the presence of menstrual irregularities constitutes a prerequisite for the diagnosis of PCOS, we wonder if th...

ea0020p659 | Reproduction | ECE2009

Adiponectin levels and its relation to indices of insulin resistance in women with PCOS

Bozic Ivana , Macut Djuro , Popovic Bojana , Isailovic Tatjana , Ognjanovic Sanja , Petakov Milan , Elezovic Valentina , Damjanovic Svetozar

Objectives: Insulin resistance and hyperinsulinaemia are implicated in the pathogenesis of the polycystic ovary syndrome (PCOS) in the majority of the cases. Adiponectin is adipose tissue-specific protein and its correlation with insulin resistance is well established. The aim of the study was to access the correlation between adiponectin and anthropometric and metabolic parameters in a group of women with PCOS.Methods: Thirty-one women with PCOS (age: 2...

ea0020p660 | Reproduction | ECE2009

Preterm menopause and some of its related factors among over 40 years old women

Sadeghi Mahbobeh , Mirmiran Parvin , Sadeghian Saeid

Goal: To study Prevalence of Preterm menopause and its relation to obesity; to study relation of DM and the age of the first menorrhoea among women over 40 years old.Materials and methods: The study was performed on 640 menopause women (with no menorrhoea during the past 12 months) who participated in ‘sugar and lipid’ study of Tehran during 2006 to 2008. With no history of hysterectomy. Preterm menopause was defined as menopause before 48 year...

ea0020p661 | Reproduction | ECE2009

Does idiopathic hyperandrogenemia exist?

Kursad Unluhizarci , Emin Capak Mehmet , Fatih Tanriverdi , Zuleyha Karaca , Fahrettin Kelestimur

Some of the patients with hyperandrogenemia are characterized by regular cycles and normal ovaries on USG and named as idiopathic hyperandrogenemia (IHA). To clarify the pathophysiology of IHA we have investigated both the adrenal and ovarian functions, and insulin resistance in 20 patients with IHA and 10 healthy women. The study was approved by the local Ethics Committee. The patients have at least one increased serum androgen. ACTH stimulation test, buserelin test and an OG...

ea0020p662 | Reproduction | ECE2009

Ovulation induction and multicentric follow-up of 21 pregnancies in 14 patients

Villaret Laure , Chabre Olivier , Caron Philippe , Young Jacques , Quenard Nicole , Bry Helene , Hoffmann Pascale

Objective: To determine the outcome of pregnancy in hypopituitary women.Background: Rare situation, not studied enough: only two previous studies concerning 9 and 19 patients (Overton et Hall).Design: Multicentric, descriptive, prospective and retrospective study.Material and methods: Twenty-one pregnancies were registered in 14 hypopituitary women (defined as gonadotropic deficiency associated with at least ...

ea0020p663 | Reproduction | ECE2009

The effects of subclinical hypothyroidism treatment in PCOS women with metabolic syndrome

Velija-Asimi Zelija

Aim: The aim of study was to explore the effects of subclinical hypothyroidism (SH) treatment in PCOS women with metabolic syndrome (MS) and PCOS women without MS.Methods: The 34 PCOS women were divided in two groups: 1) PCOS women with SH and the MS (n=23, age 32.9±9 years, BMI=31.2±3.1 kg/m2; waist size=87 cm) and 2) PCOS women with SH without MS (n=11, age 30.5±7.5 years, BMI=23.4±1.5 kg/m2; wais...

ea0020p664 | Reproduction | ECE2009

Total ghrelin levels in obese and non-obese patients with polycystic ovary syndrome

Pejkovic Danica , Micic Dragan , Sumarac-Dumanovic Mirjana , Cvijovic Goran , Zoric Svetlana , Kendereski Aleksandra

Polycystic ovary syndrome (PCOS) is associated with adiposity and metabolic changes predisposing to insulin resistance. Because the recently discovered GH secretagogue, ghrelin is intimately involved in the control of appetite and weight regulation, we investigate ghrelin levels in non-obese and obese PCOS patients. Ten obese (BMI=32.50±1.57 kg/m2) and ten non-obese (BMI=20.45±0.51 kg/m2) patients with PCOS and their respective controls were eva...

ea0020p665 | Reproduction | ECE2009

Our experience in treatment of infertility in patients with Klinefelter syndrome (mosaic karyotype 46,XY/47,XXY)

Vinogradov Igor , Kalinchenko Svetlana , Mskhalaya George , Gusakova Dariya , Afanasjeva Lubov

Introduction: Until 1996, men with Klinefelter syndrome (KS) were considered to be infertile, but with the development of testicular sperm extraction (TESE) and intracytoplasmatic sperm injection (ICSI) it is now possible to extract viable spermatozoa from the testes using surgical biopsy for the future injection into an ovum. A minority of men with KS have viable sperm in their ejaculate and might, therefore, be able to provide native sperm for ICSI.Mat...

ea0020p666 | Reproduction | ECE2009

Periodontal disease in polycystic ovary syndrome

Dursun Erhan , Guncu Guliz , Cinar Nese , Harmanci Ayla , Ozbek Murat , Karabulut Erdem , Tozum Tolga F , Kilinc Kamer , Akalin F Alev , Yildiz Bulent O

Background: Periodontal disease (diseases of the tissues around teeth) and polycystic ovary syndrome (PCOS) are common disorders in women with a significant public health impact. Both disorders appear to be associated with diabetes and cardiometabolic risk. There are no published data regarding periodontal disease in PCOS.Objective: To determine periodontal status in women with PCOS compared to healthy women.Methods: We studied 25 ...

ea0020p667 | Reproduction | ECE2009

Selected cytokines are associated with markers of insulin resistance in polycystic ovary syndrome

Stanicka Sona , Vrbikova Jana , Haluzik Martin , Hill Martin , Dvorakova Katerina , Grimmichova Tereza , Vondra Karel

The polycystic ovary syndrome (PCOS) is associated with features of the insulin resistance syndrome and altered glucose homeostasis. Factors that play an important role in these processes are still emerging. Pro-inflammatory cytokines may be involved in development of insulin resistance in PCOS. The purpose of this study was to determine if a relationship exists between interleukin-6 (IL-6), interleukin-8 (IL-8), monocyte chemoattractant protein-1 (MCP-1), hepatocyte growth fa...