Introduction: Until 1996, men with Klinefelter syndrome (KS) were considered to be infertile, but with the development of testicular sperm extraction (TESE) and intracytoplasmatic sperm injection (ICSI) it is now possible to extract viable spermatozoa from the testes using surgical biopsy for the future injection into an ovum. A minority of men with KS have viable sperm in their ejaculate and might, therefore, be able to provide native sperm for ICSI.
Materials and methods: We hold 4 successful programmed in vitro fertilization (IVF)/ICSI cycles to couples with mans factor of infertility because of KS (karyotype 46,XY/ 47,XXY) in our clinic.
The results of initial spermograms presented in Table 1.
|Volume, MI||Concnetration million/ml||Motility, % AB (A + B)||Normal Morphology, %|
|No 1||1.2||0.4||2 (0+2)||4|
|No 3||0.8||1.1||12 (2+10)||9|
Results: We used native sperm in 3 patients, and spermatozoa in one case were received by multifocal TESE.
IVF/ICSI cycles were routine. ICSI was performed for all cases. The embryo on the stage of 6 or more blastomeres underwent preimplantation genetic diagnostics (PGD). The aim of this diagnostics was to select only female embrios for the further implantation. Pregnancy and labor of KS patients wives were ordinary and didnt differ from normal. Children were examined by pediatrician, no abnormalities were found. All children had a normal karyotype.
Conclusion: Pregnancy can be achieved in KS patients families, using IVF/ICSI method.
25 - 29 Apr 2009
European Society of Endocrinology