Searchable abstracts of presentations at key conferences in endocrinology
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12th European Congress of Endocrinology

Poster Presentations

Signal transduction

ea0022p720 | Signal transduction | ECE2010

Role of filamin-A in the regulation of dopamine D2 receptor localization and signaling in a lactotroph cell model

Mantovani Giovanna , Peverelli Erika , Ferrero Stefano , Vitali Eleonora , Beck-Peccoz Paolo , Spada Anna , Lania Andrea

Dopamine agonists (DA) inhibit prolactin (PRL) secretion and expression on the pituitary lactotrophs by binding dopamine D2 receptor. Prolactinomas are the most common secreting pituitary adenomas, and DA are the first choice for their treatment because they reduce PRL levels and tumor size. However, a subset of patients is resistant to DA. The mechanisms involved in DA resistance are not fully understood, although decreased expression of D2 receptor or altered signal transduc...

ea0022p721 | Signal transduction | ECE2010

Lysyl oxidase interacts with AGEs signaling to modulate collagen synthesis in polycystic ovarian tissue

Papachroni Katerina , Piperi Christina , Levidou Georgia , Korkolopoulou Penelope , Pawelczyk Leszek , Diamanti-Kandarakis Evanthia , Papavassiliou Athanasios

The connective tissue components, collagen types I, III and IV, which surround the ovarian follicles, undergo drastic changes during ovulation. Abnormal collagen synthesis and increased volume and density of ovarian stroma characterise the polycystic ovary syndrome (PCOS). Physiologically, collagen synthesis in ovarian follicles is partly regulated by lysyl oxidase (LOX), which catalyzes the collagen and elastin cross-linking and plays indispensable role in the organization of...

ea0022p722 | Signal transduction | ECE2010

Peroxisome-proliferator-activated receptor gamma is required for modulating endothelial inflammatory response through a novel nongenomic mechanism

Cantini Giulia , Lombardi Adriana , Borgogni Elisa , Francalanci Michela , Ceni Elisabetta , Gelmini Stefania , Poli Giada , Galli Andrea , Serio Mario , Forti Gianni , Luconi Michaela

Besides their well-known anti-diabetic effects, the peroxisome proliferator-activated receptor γ (PPARγ) thiazolidinedione ligands (TZD) have been suggested to also display anti-inflammatory properties. The receptor role in mediating such effects is far from being elucidated. Here, we demonstrate that PPARγ is necessary for TZD to interfere with TNFα and IFNγ inflammatory activity in human endothelial cells. Different PPARγ ligands similarly inhib...

ea0022p723 | Signal transduction | ECE2010

The β subunits of heterotrimeric G-protein in thyroid plasma membranes

Adler Grazyna , Piotrowska Urszula , Kozicki Ireneusz

TSH regulates thyroid function trough the receptor coupled to heterotrimeric G-protein composed of α, β and gamma subunits. The activation of α s /adenylyl cyclase mediated pathway accounts for most of TSH biological effects. TSH can also induce the other G-α protein mediated activities. Five different isoforms of β subunit have been described and it can be assumed that specific α-β combination are essential for functional differentiation of ...

ea0022p724 | Signal transduction | ECE2010

Spatial and temporal regulations of MAPKinases ERK1/2 in the somatolactotroph cell line GH4C1

Zeiller Caroline , Blanchard Marie-Pierre , Pertuit Morgane , Thirion Sylvie , Enjalbert Alain , Barlier Anne , Gerard Corinne

Somatotroph adenomas are characterized by growth hormone hypersecretion and cell proliferation. We have previously identified crosstalk between the cAMP pathway and the MAPKinases ERK1/2 cascade and shown the central place of the MAPKinases in the control of the pituitary function in GH4C1 somatolactotroph cell line.The spatio-temporal organization of the signalling partners governs signalling fidelity and encodes the specificity of biological responses....

ea0022p725 | Signal transduction | ECE2010

Molecular origin of idiopathic central hypogonadism: variable impairment of two signal-transduction pathways due to PROKR2 mutations

Libri Domenico Vladimiro , Bonomi Marco , Busnelli Marta , Kleinau Gunnar , Krausz Csilla , Beck-Peccoz Paolo , Agostino Sinisi Antonio , Persani Luca , ICH study group of the Italian Societies for Endocrinology and Paediatric Endocrinology

ICH is a heterogeneous disease mainly due to functional defects of GnRH neurons. Recent studies have described the importance of prokineticin pathway in the pathogenesis of these defects. In a series of 166 ICH patients we found germline mutations affecting this pathway in about 10% of the cases. PROKR2 gene analysis revealed 3 novel (V158I, T260M, V334M) and 4 already known (L173R, R268C, V274D, V331M) mutations affecting different transmembrane and intracellular regions of t...