Searchable abstracts of presentations at key conferences in endocrinology
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15th International & 14th European Congress of Endocrinology

Poster Presentations

Growth hormone IGF axis - basic

ea0029p984 | Growth hormone IGF axis - basic | ICEECE2012

Evidence of direct mitogenic activity of insulin and the insulin receptor in prostate cancer cell lines

Weinstein D. , Laron Z. , Werner H.

Background: In addition to its normal spectrum of metabolic effects, insulin has been identified as a growth factor capable of promoting mitogenic activities. Thus, hyperinsulinemia, a consequence of insulin resistance, is regarded as a potential risk factor for the development of cancer in patients with diabetes. However, the mechanism of action of insulin in prostate cancer has not yet been completely elucidated. The aim of this study was to investigate whether insulin can d...

ea0029p985 | Growth hormone IGF axis - basic | ICEECE2012

Insulin receptor compensates for IGF1R inhibition and induces mitogenic activity in prostate cancer cell lines

Weinstein D. , Laron Z. , Werner H.

Background: IGF1R targeting emerged in recent years as a promising therapeutic approach in prostate cancer (PCa). The insulin receptor (IR) shares high structure homology with the IGF1R and activates similar signaling cascades. In recent studies we have shown that insulin’s mitogenic activities in PCa cells are mediated via the IR. Given the central role of IR in regulation of metabolism, most anti-IGF1R therapeutic strategies were designed to specifically inhibit IGF1R, ...

ea0029p986 | Growth hormone IGF axis - basic | ICEECE2012

Designing long acting recombinant proteins: from bench to clinics

Fares F.

One major issue regarding the clinical use of many peptides is their short half-life span in the body, due to the rapid clearance from the circulation. Thus, at the clinical level, there is a need for a regime of frequent injections of the peptides into the patients to overcome this low stability factor. To overcome this problem, we succeeded to add the signal sequence of O-linked oligosaccharides to the coding sequence of the hormones. The cassette gene that has been u...

ea0029p987 | Growth hormone IGF axis - basic | ICEECE2012

GH harmonisation: it sounds simple…,?

Menheere P.

Objective: The concentration of GH (hGH) is important in the attribution of hGH therapy, for which in The Netherlands a national committee is responsible. Without this authorization re-imbursement is impossible. Therapy is permitted if all hGH concentrations after two stimulation tests are below 20 mU/l and in some clinical conditions independent of hGH. However, different assays produce different results and nationwide hGH results varied with CV ~30%. Consequently, authorizat...

ea0029p988 | Growth hormone IGF axis - basic | ICEECE2012

Plasma ghrelin levels appeared to be elevated in patients with medium-chain acyl-CoA dehydrogenase deficiency and glutaric aciduria type II: evidence for that acyl-CoA is the substrate for ghrelin acylation

Akamizu T. , Sakura N. , Shigematsu Y. , Tajima G. , Ohtake A. , Hosoda H. , Iwakura H. , Ariyasu H. , Kangawa K.

Ghrelin requires a fatty acid modification for binding to the GH secretagogue receptor. Octanoylation of the Ser3 residue of ghrelin is essential for ghrelin-mediated stimulation of GH secretion and regulation of energy homeostasis via increased food intake and adiposity. Other than octanoylation (C8:0), the hormone is subject to other types of acyl modification, decanoylation (C10:0), and possibly decenoylation (C10:1). The fatty acid substrate that contributes to ghrelin acy...

ea0029p989 | Growth hormone IGF axis - basic | ICEECE2012

Ghrelin suppresses angiotensin II-induced tissue damages by reducing oxidative stress

Fujimura K. , Wakino S. , Minakuchi H. , Tokuyama H. , Hayashi K. , Itoh H.

Objectives: Angiotensin II (AngII) induces renal premature senescence by multiple mechanisms including by increasing oxidative stress. Recent study revealed that growth hormone secretagogue ghrelin exerts anti-senescence effects. In this study, we examined whether ghrelin inhibits AngII-induced renal senescence and damages.Methods: Renal senescence was induced by infusion of Ang II in C57BL/6 mice with osmotic mini-pump. Ghrelin was administered by the d...

ea0029p990 | Growth hormone IGF axis - basic | ICEECE2012

The effect of ghrelin on the mitochondrial dysfunction in muscle and physical disability associated with aging

Mitsuishi M. , Miyashita K. , Muraki A. , Tamaki M. , Tanaka K. , Itoh H.

Aim: We investigated the influence of mitochondrial dysfunction associated with aging on physical performance in mice, and the effect of hormone replacement on physical performance.Method: Using 8, 20, 50 and 100 week-old C57bl6 mice, we evaluated skeletal muscle mass, mitochondrial activity, glucose tolerance and physical performance (muscle power and exercise endurance). In addition, we treated 50-week-old mice with ghrelin or insulin like growth facto...

ea0029p991 | Growth hormone IGF axis - basic | ICEECE2012

Health related quality of life and psychological functioning of short statured German children and adolescents: findings form the quality of life in short stature youth study

Quitmann J. , Behncke J. , Chaplin J. , Pleil A. , Herdman M. , Rohenkohl A. , Mimoun E. , Wollmann H. , Bullinger M. , Power M.

Background: About 3% of all children are short statured. Clinically GH deficiency (GHD) or idiopathic short stature (ISS) is frequently diagnosed. GH (GH−) treatment, which is primarily indicated for GHD patients, is provided not only to increase height, but also to improve psychological functioning and raise health related quality of life. The present study examines these psychosocial outcomes in young German patients and their parents, and analyzes differences accordin...

ea0029p992 | Growth hormone IGF axis - basic | ICEECE2012

C677T methylenetetrahydrofolate reductase gene polymorphism and colorectal neoplasms in acromegalic patients

Torre M. , Russo G. , Ragonese M. , Giandalia A. , Puglisi S. , De Menis E. , Arnaldi G. , Romanello G. , Cucinotta D. , Trimarchi F. , Cannavo S.

Introduction: Acromegalic patients have an increased risk of developing colorectal neoplasms (CN). A common polymorphism (C677T) in gene coding for methylenetetrahydrofolate reductase (MTHFR), a key enzyme in folate metabolism and in DNA synthesis, reparation and methylation, has been associated with CN risk in general population, but its role in acromegalic patients has never been explored yet.Aim: To investigate the contribution of MTHFR C677T polymorp...

ea0029p993 | Growth hormone IGF axis - basic | ICEECE2012

Clinical characteristics and outcome of acromegaly induced by ectopic secretion of GHRH: a French nationwide series of 21 cases

Garby L. , Caron P. , Claustrat F. , Chanson P. , Tabarin A. , Rohmer V. , Arnault G. , Bonnet F. , Chabre O. , Christin Maitre S. , Du Boullay H. , Murat A. , Nakib I. , Sadoul J. , Sassolas G. , Claustrat B. , Raverot G. , Borson Chazot F.

Context: Ectopic GHRH secretion is a rare cause of acromegaly described mainly in isolated case reports.Setting: From the registry of the sole laboratory performing plasma GHRH assays in France, we identified cases of ectopic GHRH secretion presenting with acromegaly between 1983 and 2008.Patients: In total, 21 patients aged 14–77 years, from 12 French hospitals. Median GHRH was 548 ng/l (270–9779)....

ea0029p994 | Growth hormone IGF axis - basic | ICEECE2012

Comparison of intuitiveness, ease of use and preference in two disposable GH injection devices

Kappelgaard A. , Qvist M. , Winter F. , Muller B. , Pfaffle R.

Aim: GH is used to treat short stature in children with GH deficiency (GHD), Turner syndrome (TS) and born small for gestational age (SGA). As GH is injected daily, treatment adherence may be problematic. To maximise adherence, improvements in injection devices have been targeted. This report compares intuitiveness and ease of use of two disposable GH devices: Norditropin NordiFlex (NF; Novo Nordisk A/S, Bagsværd, Denmark) and GoQuick (GQ, Pfizer, Inc., New York, USA).</p...

ea0029p995 | Growth hormone IGF axis - basic | ICEECE2012

Diabetic retinopathy and GH the case of acromegaly

Azzoug S. , Namani S. , Adem A. , Chentli F.

Although GH has been implicated in the pathogenesis of retinopathy in diabetic subjects, the frequency of retinopathy in diabetic acromegalic subjects is deemed to be rare. The aim of our study is to evaluate the prevalence of retinopathy in hypersomatotropic patients.Subjects and methods: In total, 40 acromegalic diabetic patients (21 M, 19 F, mean age=50 years) underwent funduscopy to search for diabetic retinopathyResults: Among...

ea0029p996 | Growth hormone IGF axis - basic | ICEECE2012

Assessing the quality of life of children and adolescents with short stature: development and psychometric testing of the QOLISSY: instrument

Quitmann J. , Bullinger M. , Chaplin J. , Rohenkohl A. , Wollmann H. , Mimoun E. , Pleil A. , Herdman M. , Power M.

In children whose height is substantially below norms, behavioural and emotional problems can be experienced. When evaluating the outcomes of treatments or making treatment decisions, health related quality of life (HrQoL) should be taken into consideration. At present, no standardised HrQoL instrument for short stature youth exists. Through consensus building in five countries, the objective of this study was to develop and psychometrically test a growth-related HrQoL instrum...

ea0029p997 | Growth hormone IGF axis - basic | ICEECE2012

Factors affecting IGF1 response to GH during transition

Thankamony A. , Capalbo D. , Heywood J. , Williams R. , Ong K. , Dunger D. , Simpson H.

Background: GH replacement during the childhood-adult transition period is important for somatic maturation. The aim of the study was to explore the factors influencing IGF1 response to GH during transition.Methods: KIMS (Pfizer International Metabolic Study) database in UK was interrogated, and 98 patients (55 male, median age 20.7 years (15.7–25.8)) with childhood-onset GH deficiency (Co-GHD), who were started on adult dose of GH during transition...

ea0029p998 | Growth hormone IGF axis - basic | ICEECE2012

Quality of life in adult patients with GH deficiency and neurosecretory dysfunction at the end of GH therapy

Quitmann J. , Kammerer U. , Bullinger M. , Schofl C. , Dorr H.

GH is used primarily to increase adult height in short children with GHD/NSD with little knowledge about the impact of GH on health-related quality of life (HRQoL). Therefore this study was carried out to evaluate HRQoL in adult patients with childhood onset GHD/NSD at the end of GH therapy.HrQoL was measured by patient self-report using two generic questionnaires, SF36 and NHP. Patient data were compared to representative norm data. Clinical data as wel...

ea0029p999 | Growth hormone IGF axis - basic | ICEECE2012

Hypertension and cardiovascular risk factors in GH deficiency

Fedala N. , Chentli F. , Haddam A.

Studies showed an increase of cardiovascular risk (CVR) and reduction of the life expectancy observed in the anterior pituitary insufficiency (API) subjects. The morbimortality would be related to GH deficiency not substituted.Aim: To seek the frequency of the hypertension and analyse the predictive factors in subjects with API.A retrospective study relating 55 (47 women, eight men) subjects with API: interrupted pituitarystalk n:6...

ea0029p1000 | Growth hormone IGF axis - basic | ICEECE2012

Anti-androgen flutamide alters gene expression in the porcine fetal ovary

Knapczyk-Stwora K. , Durlej M. , Duda M. , Galas J. , Koziorowski M. , Slomczynska M.

In pigs, the assembly of primordial follicles and their subsequent transition to the primary stage occur in the late gestational and neonatal period. Previously, we reported the presence of androgen receptors in the porcine fetal ovary on different days of gestation, which suggests a fetus capacity to respond to androgens. Since testosterone was detected in the plasma of porcine female fetuses during the second half of pregnancy, it is possible that androgens are involved in p...

ea0029p1001 | Growth hormone IGF axis - basic | ICEECE2012

Relationship between GH activity and reproductive hormones: a randomized cross-over study in healthy male volunteers treated with GH and a GH receptor antagonist for three weeks

Andreassen M. , Frystyk J. , Juul A. , Faber J. , Kristensen L.

Introduction: The GH/insulin-like growth factor 1 (IGF1) system may modulate the pituitary-gonadal axis in males. Direct pituitary and/or testicular effects might be involved, as well as indirect effects on sex hormone binding globulin (SHBG) and aromatase activity.Methods/design: Nine healthy male volunteers (mean age 37, range 29–49 years) were treated in random order with increasing doses of GH for three weeks (1st week 0.01, 2nd week 0.02, 3rd 0...

ea0029p1002 | Growth hormone IGF axis - basic | ICEECE2012

Baseline characteristics and differences in short- and long-term response to rhGH between GHD adults with craniopharyngioma and nonfunctioning pituitary adenoma

Profka E. , Giavoli C. , Bergamaschi S. , Ferrante E. , Malchiodi E. , Sala E. , Verrua E. , Spada A. , Beck-Peccoz P.

Patients with craniopharyngioma (CP) are more often operated by transcranial route than patients with nonfunctioning pituitary adenoma (NFPA), have higher prevalence of pituitary deficiencies, are more obese and dyslipidemic and have a higher mortality rate. A previous study in a large group of GHD subjects, showed that the effects of 2-year rhGH replacement are similar in patients operated for CP and in patients operated for NFPA, except for less reduction in fat mass in CP p...

ea0029p1003 | Growth hormone IGF axis - basic | ICEECE2012

A new somavert (pegvisomant) 30 mg/ml formulation compared to the currently marketed 15 mg/ml formulation: pharmacokinetics, safety, and tolerability in healthy subjects

Jen J. , LaBadie R. , Liang Y. , Crownover P. , Gao X. , Hey-Hadavi J.

Introduction: Pegvisomant is indicated for acromegaly. Doses >20 mg are not commercially available, although daily doses up to 30 mg are approved.Objectives: 1. To estimate the relative bioavailability (relBA) of single-dose pegvisomant s.c. administrations, one injection of 30 mg/ml (1×30) vs two injections of 15 mg/ml (2×15), and 2. To evaluate their pharmacokinetics (PK), safety and tolerability.Methods: This was a...

ea0029p1004 | Growth hormone IGF axis - basic | ICEECE2012

Thyroid function during long-term GH treatment in children affected by idiopathic GH deficiency

Ciresi A. , Guarnotta V. , Perrone C. , Galluzzo A. , Giordano C.

Alterations in the thyroid axis have been reported following GH therapy both in adults and children with GH deficiency (GHD), even if the clinical significance of these changes, in small and short-term studies, remains uncertain. To evaluate the impact of GH replacement on thyroid status in a large selected cohort of prepubertal children with idiopathic GHD during a 3-years follow-up. Study outcome considered FT3, FT4 and TSH modifications (delta) during therapy and their corr...

ea0029p1005 | Growth hormone IGF axis - basic | ICEECE2012

Variations of IGF1 in GH deficiency and Algerian health children

Fedala N. , Chentli F. , Haddam A. , Griene L.

IGF1 represent an important key player in several physiologic process, so it can be implicated in different pathologies among delays of growth. A comparative study was realized comparing a group of healthy Algerian children of normal size (n: 266) and a group presenting a GH deficiency (GHD n: 107)to a group of healthy westerners children of normal size(Serie of ROSENFIELD).The results are as follows: Before the age of 04 years, there is a ...

ea0029p1006 | Growth hormone IGF axis - basic | ICEECE2012

Association Turner's syndrome and GH deficiency

Oueslati I. , Hadj Ali I. , Khiari K. , Mchirgui N. , Ben Abdallah N.

One of the most clinical characteristic of Turner’s syndrome is the final short stature.In order to cure this handicap, several teams were interested to treat these patients by the GH.We report two cases of Turner syndrome associated with GH deficiency.In the first, a 17 years old girl, having a delayed growth lower than −4 S.D. associated with delayed puberty and dysmorphic syndrome...