Male Reproduction

ea0029p1007 | Male Reproduction | ICEECE2012

The homologous hormones lutropin and choriogonadotropin are interacting differently with the LH/CG receptor

Grzesik P. , Teichmann A. , Kreuchwig A. , Furkert J. , Rutz C. , Wiesner B. , Schulein R. , Gromoll J. , Krause G.

Activation of the human LH/CG receptor (LH/CGR) by lutropin (LH) and choriogonadotropin (CG) is essential in the human reproduction. Deletion of the Exon10 (LH/CGR-delExon10) resulting in a lack of 27 amino acids within the extracellular hinge-region of LH/CGR causes Leydig cell hypoplasia type II. To clarify why this deletion impairs LH but not CG action, we investigated the molecular determinants of LH/CGR activation elucidating the different behaviour of both hormones.<...

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ea0029p1008 | Male Reproduction | ICEECE2012

Differential expression of pseudoautosomal region genes in patients with Klinefelter syndrome

Zuccarello D. , Speltra E. , Perilli L. , Selice R. , Ferlin A. , Foresta C.

Klinefelter syndrome (KS) was first described in 1942 and the cause for the syndrome was identified as a supernumerary X chromosome resulting in the karyotype 47,XXY. 8090% of KS cases bear this karyotype, whereas the remaining exhibit (in decreasing frequency) varying mosaicism (e.g. 47,XXY/46,XY), carry additional sex chromosomes (48,XXXY; 48,XXYY; 49,XXXXY) or structurally abnormal X chromosomes. The prevalence of KS is up to 1 in 500 boys, and it is the most common c...

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ea0029p1009 | Male Reproduction | ICEECE2012

Rab-like 2 (RABL2) function in male fertility, intra-flagella transport and sperm tail assembly

Lo J. , O'Bryan M.

Infertility affects 1 in 20 Australia men of reproductive age, and for the majority, the causes remain unknown. In an effort to identify addition key pathways and proteins involved in male fertility we undertook a random mouse mutagenesis screen. In the process we identified the Mot1 line which carries a point mutation in the Rabl2 gene. Mot1 homozygous male are sterile, have a reduced daily sperm output, short sperm tails and drastically reduced progressive sperm motil...

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ea0029p1010 | Male Reproduction | ICEECE2012

Prevalence of olfactory and other developmental anomalies in patients with central hypogonadotropic hypogonadism

Valle E. Della , Vezzani S. , Rochira V. , Granata A. , Carani C. , Genovese E. , Simoni M.

Introduction: Hypogonadotropic hypogonadism (HH) is a heterogenous disease caused by mutations in several genes. Based on the presence of hyposmia/anosmia it is distinguished into Kallmann syndrome and isolated HH. The prevalence of other developmental anomalies is not well established.Methods: We studied patients with HH (29 males, 3 females, mean age 41.5), 9 with familial and 23 sporadic HH (29 congenital, 3 adult-onset), by physical examination, smel...

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ea0029p1011 | Male Reproduction | ICEECE2012

Abstract withdrawn....

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ea0029p1012 | Male Reproduction | ICEECE2012

Total Testosterone levels and predisposing criteria for metabolic syndrome in healthy young men

Hlazkova A. , Murashko N. , Danilova L. , Romanouski A. , Yaroshevich N. , Raduk D. , Dashkevich E. , Pishchik V.

Object of the study: To evaluate total testosterone (TT) levels in healthy young men and determine its possible influence on metabolic syndrome risk factors.Materials and methods: One hundred and twenty-six practically healthy young men, mean age 20.16±1.42 years old (1829) were included in the study. Individuals with any clinical manifestations suspicious for hypogonadism, chronic liver and kidney diseases in the anamnesis or receiving any ki...

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ea0029p1013 | Male Reproduction | ICEECE2012

Functional status of pituitary–gonads axis in males with obesity

Urmanova Y. , Nabieva I.

Aim: to study functional status of pituitarygonads (PG) axis in males with obesity.Materials and methods: We evaluated 15 males with obesity without other associated pathology. Mean age of patients was 28.3 years old. Control group constituted by 20 healthy men with different age.All patients underwent clinical and biochemical evaluations including endocrine check, lipids profile, hormonal profile (LH, FSH, prolactin, ...

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ea0029p1014 | Male Reproduction | ICEECE2012

Deletion of FSTL3 increases testicular size and delays testicular regression

Mukherjee A. , Oldknow K. , Seebacher J. , Villen J. , Goswami T. , Gygi S. , O'Shaughnessy P. , Schneyer A.

Follistatin-like 3 (FSTL3) is a secreted glycoprotein known to act as an inhibitor of TGFβ ligands such as activin and myostatin. Like activin, FSTL3 is expressed strongly in the testis but the role FSTL3 plays in testis development and physiology is not clearly understood. Here we show that FSTL3 is a key regulator of testicular size and a promoter of age-dependent testicular regression. FSTL3 deletion in mice leads to the development of enlarged testes that show delayed...

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ea0029p1015 | Male Reproduction | ICEECE2012

High prevalence and interaction of hypogonadism and the metabolic syndrome in long-term survivors with germ cell tumours

Zitzmann M. , Gottardo F. , Grunwald L. , Puhse G. , Secker A. , Kliesch S.

Introduction and objectives: Testicular cancer survivors (TCS) are at a putative risk to develop premature testosterone deficiency (TD). The clinical and biochemical components of TD and the metabolic syndrome have not been thoroughly described in such patients.Material and methods: Prospective clinical trial: 163 TCS took part in the study, clinical parameters, sex hormones and biochemical values were assessed in fasting blood samples, MS was defined ac...

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ea0029p1016 | Male Reproduction | ICEECE2012

Mutation analysis of NR5A1 gene encoding steroidogenic factor 1 in cryptorchidism and male infertility

Ferlin A. , Vinanzi C. , Zuccarello D. , Ghezzi M. , Foresta C.

The gene NR5A1, which encodes steroidogenic factor 1 (SF-1), is a pivotal transcriptional regulator of genes involved in adrenal and gonadal function, including several steroidogenic enzymes and key genes necessary for male sex determination and differentiation, testicular descent and reproduction (such as SOX9, AMH, INSL3, and AR). The most severe phenotypes associated with NR5A1 mutations include gonadal dysgenesis, disorders of sex development (DSD) and adrenal insuf...

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ea0029p1017 | Male Reproduction | ICEECE2012

The Muenster EXAKT project: cardiovascular risk factors in Klinefelter patients and healthy controls

Zitzmann M. , Bongers R. , Werler S. , Tuttelmann F. , Rittstieg C. , Edelbrock L. , Gromoll J. , Kliesch S.

Background and Aim: Klinefelter syndrome (47,XXY; KS) is a very common chromosomal disorder, affecting 1:600 men. Klinefelter men have been described to exhibit clinically relevant metabolic patterns related to a pro-inflammatory status, resulting in a high prevalence of insulin resistance and cardiovascular impairment. Testosterone deficiency in form of primary hypogonadism is a common feature in these men.EXAKT (epigenetics, X-chromosomal features and ...

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ea0029p1018 | Male Reproduction | ICEECE2012

Cellular distribution, regulated expression and functional role of the anorexigenic peptide, NUCB2/nesfatin-1, in the testis

Garcia-Galiano D. , Pineda R. , Ilhan T. , Castellano J. , Ruiz-Pino F. , Sanchez-Garrido M. , Vazquez M. , Sangiao-Alvarellos S. , Romero-Ruiz A. , Pinilla L. , Dieguez C. , Gaytan F. , Tena-Sempere M.

Nesfatin-1, product of the precursor NEFA/nucleobindin 2 (NUCB2), was initially identified as anorectic hypothalamic neuropeptide, acting in a leptin-independent manner. In addition to its central role in the control of energy homeostasis, evidence has mounted recently that nesfatin-1 is also produced in peripheral metabolic tissues, such as pancreas, adipose and gut. Moreover, nesfatin-1 has been shown to participate in the control of body functions gated by whole-body energy...

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ea0029p1019 | Male Reproduction | ICEECE2012

Neurocognitive phenotype and personality profile in men with Klinefelter syndrome and their vulnerability to psychiatric symptoms

Jensen A. , Pedersen A. , Bojesen A. , Hertz J. , Wallentin M. , Ostergard J. , Gravholt C.

Background: Klinefelter syndrome (KS) is associated with increased risk of psychiatric disease and behavioral problems, as well as social problems. The background for these risks is not known.Aim: The aim was to describe the cognitive function, personality traits and the vulnerability to psychiatric symptoms in patients with KS.Methods: Fourty one KS patients and 41 age- and educational-matched control subjects participated in the ...

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ea0029p1020 | Male Reproduction | ICEECE2012

FSHB promotor polymorphism influences male reproductive parameters

Tuttelmann F. , Grigorova M. , Terwort N. , Punab M. , Gromoll J. , Laan M.

Introduction: Recently, a single nucleotide polymorphism (SNP) in the FSH (FSH)-β gene (FSHB −211G>T, rs10835638) that leads to reduced mRNA transcription was associated with serum FSH levels in an Estonian cohort of young men (Grigorova et al., Hum Reprod 2008). Further investigations showed an increased frequency of the T-allele in patients with oligozoospermia compared to men with normozoospermia (Grigorova et al., JCEM 2010). Another Bal...

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ea0029p1021 | Male Reproduction | ICEECE2012

Side effect profile of long-term treatment of hypogonadal men with testosterone undecanoate

Saad F. , Haider A. , Gooren L.

Introduction: Testosterone therapy for hypogonadal men has been used for decades. However, there are still concerns regarding the safety of this treatment, particularly in elderly men.Methods: Prospective registry study of 252 men (mean age 60.6±8.0 years), with testosterone levels between ≤3.5 ng/ml. They received parenteral testosterone undecanoate 1000 mg at day 1, week 6 and every 12 weeks thereafter for up to 66 months.<p class="abst...

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ea0029p1022 | Male Reproduction | ICEECE2012

Effect of testosterone on seminal proteome in male hypogonadism

Milardi D. , Grande G. , Vincenzoni F. , Giampietro A. , Bianchi A. , Messana I. , Pontecorvi A. , Marinis L De , Castagnola M. , Marana R.

Seminal plasma (SP) contains proteins secreted by testis, epididymis and male accessory glands, involved in the successful fertilization of the oocyte. The function of epididymis, prostate and seminal vesicles are dependent upon the presence of androgenic stimuli.To investigate the role of testosterone in the modulation of the proteomic pattern in SP, we analyzed human SP proteome comparing the panel of common seminal proteins in five fertile normogonada...

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ea0029p1023 | Male Reproduction | ICEECE2012

Comparing effects of weight loss on sexual, urinary and endothelial function, insulin resistance and quality of life in obese men with and without erectile dysfunction

Khoo J. , Chen R. , Cho L. , Tay T. , Tan E. , Au V. , Soh S. , Ng B.

Introduction: Abdominal obesity and insulin resistance are risk factors for erectile dysfunction (ED). ED is associated with hypoandrogenism, endothelial dysfunction, lower urinary tract symptoms (LUTS), and reduced quality of life (QoL). We aimed to compare effects of lifestyle modification-induced weight loss on insulin resistance, endothelial and sexual function, LUTS and QoL in obese non-diabetic men with and without ED.Methods: Seventy abdominally o...

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ea0029p1024 | Male Reproduction | ICEECE2012

Inhibitors of 5α-reductase-related side effects in patients seeking medical care for sexual dysfunction

Rastrelli G. , Corona G. , Maseroli E. , Sforza A. , Forti G. , Mannucci E. , Maggi M.

Introduction: Despite their efficacy in the treatment of benign prostatic hyperplasia (BPH) the popularity of inhibitors of 5α-reductase (5ARIs) is limited by their association with adverse sexual side effects. However, the real impact of 5ARIs on sex hormones and sexual function is controversial.Aim: The aim of this study is to investigate the role of 5ARIs therapy on hormonal parameters and sexual function in men already complaining of sexual prob...

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ea0029p1025 | Male Reproduction | ICEECE2012

Multiplex ligation dependent probe amplification analysis of KAL1, GnRH1, GnRHR, PROK2 and PROKR2 in male patients with idiopathic hypogonadotropic hypogonadism

Basaran Y. , Unal H. , Bolu E. , Sagkan R. , Taslipinar A. , Ozgurtas T. , Musabak U.

Introduction: To date, several mutations have been identified as the underlying cause of hypogonadotropic hypogonadism. However, they account for a small minority of cases, suggesting that other genes play a significant role in the pathogenesis. The aim of the present study was to examine the prevalence of KAL1, GnRH1, GnRHR, PROK2 and PROKR2 mutations. Because it has several advantages over traditional screening methods, multiplex ligation dependent probe amplification (MLPA)...

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ea0029p1026 | Male Reproduction | ICEECE2012

Cimetidine induces testosterone reduction and impairs leydig cell-microvasculature paracrine interaction in adult rats

Beltrame F. , Cerri P. , Sasso-Cerri E.

Cimetidine, an antagonist of histamine H2-receptors used for treatment of gastric ulcers, exerts antiandrogenic effect. In testes, cimetidine causes histopathological disorders in the seminiferous tubules and impairs spermatogenesis. Additionally to testosterone, Leydig cells (LC) produce several paracrine factors, including EGVEGF (endocrine gland-derived vascular endothelial growth factor) which participates in the testicular microvasculature control. Regarding the imp...

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ea0029p1027 | Male Reproduction | ICEECE2012

Gonadal status in adult male survivors of childhood cancer

Felicetti F. , Castiglione A. , Corrias A. , Chiabotto P. , Fagioli F. , Ciccone G. , Boccuzzi G. , Brignardello E.

Introduction: Survival rates in childhood cancer survivors (CCS) have enormously increased in the last 40 years. However, this improvement has been achieved at the expense of serious late effects, including the potential for severe gonadal damage. Here we report the effects of anticancer therapies on gonadal function in male long-term survivors of childhood cancer.Methods: We estimated, at the last follow up available, the prevalence of primary hypogonad...

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ea0029p1028 | Male Reproduction | ICEECE2012

Cyclooxygenase-1 (COX-1) and COX-2, differently expressed in human sperm from normal and pathological patients, might be considered molecular markers in the diagnosis of male infertility disorders

Perrotta I. , Santoro M. , Guido C. , Avena P. , Amicis F De , Cesario M. , Maris P. , Tripepi S. , Aquila S.

Varicocele and diabetes mellitus (DM) have long been recognized to impair male fertility by interfering with both sperm parameters and production, however the molecular mechanism by which these effects occur remains largely unknown. Cyclooxygenases are important in spermatogenesis, but their ultrastructural localization in sperm cells and their role in male infertility have not yet established in humans. In the present study we presented the evidence of constitutive (COX-1) an...

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ea0029p1029 | Male Reproduction | ICEECE2012

Activation of selective human sperm functions by EGF and TGF-β3 is mediated by an ERK-depended mechanism

Band G. , Etkovitz N. , Breitbart H. , Naor Z.

Introduction: The signaling involved in ligand-stimulated spermatozoa is not yet clarified. Moreover, the main question in sperm biology is the identification of the sperm ligands. Analysis of the role of MAPK in mammalian sperm physiology is a prerequisite for assigning a role for MAPK in mammalian sperm functions. The unique opportunity to study ERK1/2 in human spermatozoa, which are differentiated cells lacking active transcriptional machinery, will enable to correlate it t...

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ea0029p1030 | Male Reproduction | ICEECE2012

Leydig and sertoli cell failure in myotonic dystrophy

Passeri E. , Bugiardini E. , Sansone V. , Fulceri C. , Costa E. , Bandera F. , Borgato S. , Persani L. , Pizzocaro A. , Meola G. , Ambrosi B. , Corbetta S.

Introduction: Hypogonadism occurs in myotonic dystrophy (DM) type 1 and 2, a multisystemic autosomal dominant disorder with insulin resistance and visceral obesity. DM patients provide a model to investigate the impact of metabolic alterations on hypogonadism.Methods: We assessed Leydig and Sertoli cell functions and metabolic features in 32 DM1 (44+11 years),13 DM2 patients (54+9 years) and 32 age and BMI-matched controls.Results:...

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ea0029p1031 | Male Reproduction | ICEECE2012

Decreased osteoprotegerin levels during testosterone therapy in ageing men were associated with changed distribution of regional fat

Glintborg D. , Frederiksen L. , Hougaard D. , Brixen K. , Rasmussen L. , Andersen M.

Objective: The cardiovascular effects of testosterone treatment are debated. Osteoprotegerin (OPG) is an independent marker of cardiovascular risk. We investigated the effect of testosterone therapy on OPG levels in ageing men with low normal bioavailable testosterone levels.Design: A randomized, double-blinded, placebo-controlled study of six months testosterone therapy (gel) in 38 men aged 6078 years with bioavailable testosterone <7.3 nmol/l...

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ea0029p1032 | Male Reproduction | ICEECE2012

New insights into sperm DNA methylation: intra-and inter individual stability and a comparative analysis versus somatic cells

Krausz C. , Sandoval J. , Chianese C. , Giachini C. , Sayols S. , Esteller M.

Data about the entire sperm DNA methylome are limited to two sperm donors whereas studies dealing with a greater number of subjects focused only on a few genes or were based on low resolution arrays. This implies that information about what we can consider as a normal sperm DNA methylome and whether it is stable among different normozoospermic individuals is still missing. The definition of the DNA methylation profile of normozoospermic men, the entity of inter-individual vari...

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ea0029p1033 | Male Reproduction | ICEECE2012

Complete reversal of isolated idiopathic hypogonadothropic hypogonadism in men

Rabijewski M. , Papierska L. , Zgliczynski W.

Idiopathic hypogonadotropic hypogonadism (IHH) is caused by an isolated defect in GnRH release or action and classified as the Kallmann syndrome with associated anosmia or normosmic IHH in the presence of a normal sense of smell. Patients with IHH have absent or incomplete sexual maturation and infertility. Usually long term GnRH or gonadotropin therapy in these patients can induce spermatogenesis and puberty and is followed by testosterone therapy to maintain normal virilizat...

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ea0029p1034 | Male Reproduction | ICEECE2012

G protein-coupled estrogen receptor 1- and estrogen receptor α-dependent pathways regulate spermatocytes apoptosis

Chimento A. , Sirianni R. , Casaburi I. , Ruggiero C. , Maggiolini M. , Ando S. , Pezzi V.

In mammals, spontaneous apoptosis is observed particularly in differentiating spermatogonia and spermatocytes. We have previously demonstrated that 17β-estradiol (E2) in primary rat pachytene spermatocytes (PS) binds ESR1 and GPER1 to activate EGFR/ERK/c-Jun pathway leading to up regulation of bax, a pro-apoptotic factor. Aim of this study was to clarify the effector pathway(s) controlling spermatocytes apoptosis using as model GC-2 cells, an immortalized mouse...

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ea0029p1035 | Male Reproduction | ICEECE2012

Single nucleotide polymorphism (SNP) of endothelial nitric oxide synthase (eNOS) gene (Glu298Asp variant) in infertile men with asthenozoospermia

Buldreghini E. , Paggi F. , Gioia A. , Muti Ndelli , Macri L. , Vignini A. , Balercia G.

Objective(S): Single nucleotide polymorphism (SNP) in endothelial nitric oxide synthase (eNOS) G894T may affect the oxidative stress response. Our objective was to examine G894T SNP eNOS genotype frequencies and its potential role with sperm motility in infertile men. Through this prospective controlled study in the Andrology Unit, we have enrolled infertile (n=70) and healthy (n=60) men. Sperm motion kinetics assessed by computer assisted semen analysis (CASA), ...

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ea0029p1036 | Male Reproduction | ICEECE2012

Severe depressive symptoms and cardiovascular risk in subjects with erectile dysfunction

Fisher A. , Bandini E. , Corona G. , Ricca V. , Monami M. , Boddi V. , Balzi D. , Melani C. , Forti G. , Mannucci E. , Maggi M.

Introduction: Although penile blood flow (PBF) has been recommended as an additional diagnostic test in identifying erectile dysfunction (ED) patients at risk for latent cardiovascular disease, no study has ever assessed the possible association of PBF and the relational component of sexual function with incident major cardiovascular events (MACE).Aim: The aim of this study is to investigate whether severity of ED, PBF and other factors related to a coup...

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ea0029p1037 | Male Reproduction | ICEECE2012

The protective role of ginger against Di-(2-ethylhexyl) phthalate-induced reproductive toxicity and oxidative stress in male rabbits

Yousef M. , Hassan H. , Mohammed A. , Kamel K. , Khaled F.

The experiment was designed to study toxic effects of Di-(2-ethylhexyl) phthalate (DEHP) on semen characteristics, testosterone levels, testicular lipid peroxidation and testicular antioxidants in male New-Zealand white rabbits for 12 weeks. Ginger has been reported to be an important antioxidant. Therefore, the protective effect of ginger against DEHP-induced reproductive toxicity and oxidative stress was studied. Rabbits were orally administered the doses of ginger, DEHP and...

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ea0029p1038 | Male Reproduction | ICEECE2012

Mutations and polymorphism of FSH and LH receptors and sperm quality in men with hypergonadotropic hypogonadism without obvious testicular noxa

Di Cairano G. , Cantara S. , Collodel G. , Moretti E. , Taglianetti S. , Mazzi L. , Pacini F.

Introduction: Inactivating mutations in LH or FSH receptors (R) are known causes of gonadotrophins resistance.Aim: This study aims at evaluating the presence of mutations and polymorphisms of FSHR and LHR and sperm quality in men with hypergonadotropic hypogonadism without obvious testicular noxa.Patients and methods: We selected 8 males with elevated levels of FSH and/or LH and normal levels of testosterone. Sperm parameters were ...

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ea0029p1039 | Male Reproduction | ICEECE2012

Poor response to alprostadil ici test is associated with arteriogenic erectile dysfunction and higher risk of major adverse cardiovascular events

Rastrelli G. , Corona G. , Monami M. , Melani C. , Balzi D. , Sforza A. , Forti G. , Mannucci E. , Maggi M.

Introduction: Intracavernous alprostadil injection (ICI) test has been considered useless in assessing the vascular status of subjects with erectile dysfunction (ED).Aim: To analyze the clinical correlates of ICI test in patients with ED and to verify the value of this test in predicting major adverse cardiovascular events (MACE).Methods: A consecutive series of 2,396 men (mean age 55.9±11.9 years) attending our outpatient cli...

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ea0029p1040 | Male Reproduction | ICEECE2012

Hormonal associations and sexual dysfunctions in patients with impaired fasting glucose

Rastrelli G. , Corona G. , Balercia G. , Lotti F. , Sforza A. , Monami M. , Forti G. , Mannucci E. , Maggi M.

Introduction: The category of impaired fasting glucose (IFG) denotes a state of non-diabetic hyperglycemia, considered a risk factor of the further development of diabetes mellitus (DM) and cardiovascular (CV) diseases.Aim: The aim of the present study is to evaluate the impact of IFG on sexual health in men. In addition its effect on CV morbidity and mortality will also be addressed.Methods: A consecutive series of 3451 men (mean ...

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ea0029p1041 | Male Reproduction | ICEECE2012

The relationship between total testosterone, cognitive function, depressive behavior and sleep quality in chronic renal failure patients not on dialysis

Afsar B.

Purpose: Studies have shown that testosterone levels were associated with cognitive function, depression and sleep quality in general population. However these relationships between testosterone, cognitive function, depressive behavior, and sleep quality in chronic renal failure (CRF) patients not on dialysis were not evaluated before.Methods: All patients underwent history taking, physical examination, blood pressure measurement, routine urine and bioch...

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ea0029p1042 | Male Reproduction | ICEECE2012

Premature ejaculation and erectile dysfunction in a cohort of infertility men: clinical and ultrasonographic correlations

Lotti F. , Corona G. , Rastrelli G. , Forti G. , Jannini E. , Maggi M.

Introduction: Premature ejaculation (PE) and erectile dysfunction (ED) are considered pre-testicular causes of male subfertility. No previous study systematically evaluated the prevalence of ED and PE along with their possible correlations with clinical and colour-Doppler ultrasound (CDU) features in infertile men.Methods: Ejaculatory status, erectile function, prostatitis symptoms and psychopathological traits of 244 men complaining of couple infertilit...

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ea0029p1043 | Male Reproduction | ICEECE2012

Maternal malnutrition alters the leptin expression in the rats epididymis

Gombar F. , Sampaio F. , Ramos C.

Several papers show that maternal malnutrition can affect the reproductive capability of the offspring. Leptin is an important regulator of the reproductive system, however little is known about its role in the epididymis. The goal of this study was to evaluate whether the maternal malnutrition during lactation can alter the expression of leptin and its receptors in the epididymis of rats. Six rat dams were randomly assigned to the control group (C), with free access to a stan...

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ea0029p1044 | Male Reproduction | ICEECE2012

Scrotal and transrectal ultrasound correlates of prostatitis-like symptoms in a cohort of infertility patients

Lotti F. , Corona G. , Filimberti E. , Innocenti SDegli , Forti G. , Maggi M.

Introduction: Prostatitis-like symptoms (PLS) may originate from the prostate or from pelvic or scrotal organs. The National Institutes of Health-Chronic Prostatitis Symptom Index (NIH-CPSI) is considered the gold-standard instrument to assess PLS severity. Although several studies previously investigated the impact of prostatitis, vesiculitis or epididymitis on semen parameters, only a few studies evaluated the correlations between PLS and scrotal or transrectal colour-Dopple...

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ea0029p1045 | Male Reproduction | ICEECE2012

Elevated body mass index correlates with higher seminal interleukin 8 levels and ultrasound prostate abnormalities in infertile men

Maggi M. , Lotti F. , Corona G. , Colpi G. , Filimberti E. , Degli Innocenti S. , Mancini M. , Baldi E. , Noci I. , Adorini L. , Forti G.

Introduction: It is general acknowledgement that obesity is associated with a systemic, low-grade inflammatory state. Although the relationship between obesity and semen parameters or prostate diseases has been previously investigated, the association between body mass index (BMI), prostate inflammatory diseases and colour-Doppler ultrasound (CDU) features of the male genital tract (MGT) has been poorly studied. This study was aimed at evaluating the association between BMI an...

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ea0029p1046 | Male Reproduction | ICEECE2012

Ultrasonographic and clinical correlates of seminal plasma interleukin 8 levels in patients attending an Andrology Clinic for infertility

Maggi M. , Lotti F. , Corona G. , Mancini M. , Filimberti E. , Degli Innocenti S. , Colpi G. , Baldi E. , Noci I. , Adorini L. , Forti G.

Introduction: Interleukin 8 (IL8) is a proinflammatory cytokine highly expressed in seminal plasma. Seminal IL8 (sIL8) has been proposed as a reliable surrogate marker of prostatitis and as a potential marker of male accessory gland infection (MAGI). So far, no study systematically evaluated the correlations of sIL8 with colour-Doppler ultrasound (CDU) features of the male genital tract. Hence, we investigated these possible correlations in infertile patients with MAGI.<p ...

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ea0029p1047 | Male Reproduction | ICEECE2012

Visceral adiposity index and the effect of testosterone treatment in young men with congenital hypogonadotropic hypogonadism

Haymana C. , Sonmez A. , Aydogdu A. , Taslipinar A. , Meric C. , Basaran Y. , Baskoy K. , Tapan S. , Uckaya G. , Bolu E. , Azal O.

Introduction: Visceral adipose tissue (VAT) is closely related to metabolic derangement, such as dyslipidemia insulin resistance and type 2 diabetes. Also, there is an increase in deposition of visceral adipose tissue in hypogonadal subjects. The visceral adiposity index (VAI) is a sex-specific mathematical index, based on waist circumference (WC), body mass index (BMI), triglycerides (TG) and HDL cholesterol (HDL) levels, indirectly expressing visceral adipose function. We in...

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ea0029p1048 | Male Reproduction | ICEECE2012

Prevalence of erectile dysfunction is greater in young middle-aged HIV-infected men than in hiv-uninfected men

Madeo B. , Santi D. , Zona S. , Guaraldi G. , Rochira V.

Introduction: Erectile dysfunction (ED) is common among elderly men and patients suffering from chronic diseases, the latter probably including also HIV infection. No studies, however, compared the prevalence of ED in HIV-infected and -uninfected individuals using the IIEF-15.Aim: To compare ED prevalence in young-middle aged men with and without HIV infection using the International Index of Erectile Function (IIEF-15) questionnaire.<p class="abstex...

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ea0029p1049 | Male Reproduction | ICEECE2012

Effectiveness of gonadotropin treatment for spermatogenesis induction in hypogonadotropic hypogonadism: a possible role of androgen receptor CAG repeat polymorphism

Giagulli V. , Triggiani V. , Tafaro E. , Corona G. , Licchelli B. , Resta F. , Sabba' C. , Maggi M. , Guastamacchia E.

Prepuberal-onset HH (PRHH) and postpuberal-onset HH (PSHH) men required GnTh by means of administration of both the β human chorionic gonodadotropin (ηHCG) and the FSH to induce a complete spermatogenesis. However, the response to GnTh is always unpredictable concerning either the effectiveness or the duration of the therapy. Consequently, different studies have been carried out to identify clinical and biochemical markers that can be useful to predict the effectiven...

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ea0029p1050 | Male Reproduction | ICEECE2012

Phosphodiesterase type 5 expression in human and rat lower urinary tract tissues and the effect of tadalafil on prostate gland oxygenation in spontaneously hypertensive rats

Morelli A. , Sarchielli E. , Comeglio P. , Filippi S. , Mancina R. , Gacci M. , Vignozzi L. , Carini M. , Vannelli G. , Maggi M.

Introduction: In humans, prostate PDE5 expression was prominently localized in the endothelial and smooth muscle cells of the vascular bed, suggesting a possible action of PDE5 inhibitors on prostate blood flow.Aim: To investigate PDE5 expression in human and rat lower urinary tract (LUT) tissues and determine the effects of PDE5 inhibition with tadalafil on prostatic blood perfusion.Main outcome measures: Human vesicular-deferenti...

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ea0029p1051 | Male Reproduction | ICEECE2012

Ten years of EAA/EMQN quality control scheme for microdeletions of the Y chromosome

Tuttelmann F. , Hoefsloot L. , Patton S. , Simoni M. , Krausz C.

Introduction: Y-chromosomal azoospermia factor (AZF) microdeletions are one of the few well-established genetic causes of male infertility, specifically azoospermia or severe oligozoospermia. Since the introduction of a PCR-based method to easily detect the distinct deletion patterns, the screening of infertile men for Y microdeletions has become a very common genetic test performed frequently by a large number of laboratories. An external quality control scheme was establishe...

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ea0029p1052 | Male Reproduction | ICEECE2012

SIEDY scale 3, a new instrument to detect intrapsychic component in subjects with erectile dysfunction

Corona G. , Ricca V. , Bandini E. , Rastrelli G. , Casale H. , Jannini E. , Sforza A. , Forti G. , Mannucci E. , Maggi M.

Introduction: We previously developed and validated a structured interview (SIEDY) dealing with the organic (scale 1), relational (scale 2) and intrapsychic (scale 3) components of erectile dysfunction (ED). The aim of the present study is to identify a pathological threshold for SIEDY scale 3 and to analyze scale 3 score with biological and psychological correlates in subjects with sexual dysfunction.Method: A pathological threshold of SIEDY scale 3 sco...

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ea0029p1053 | Male Reproduction | ICEECE2012

Immunogold localization of testosterone and immunohistochemical detection of AR, SHBG, CYP1A1, ERβ and PCNA indicate a participation of steroid hormones in primordial germ cells of adult male bullfrogs

Caneguim B. , Beltrame F. , de Andrade Rosa I. , Cerri P. , Sasso-Cerri E.

In vertebrates, ARs have been detected in spermatogonia and also fetal gonocytes, confirming the importance of testosterone for the initial process of spermatogenesis. During seasonal spermatogenesis of adult bullfrogs (Lithobates catesbeianus), primordial germ (PG) cells show testosterone immunoexpression in winter; however, a weak or none testosterone immunoexpression was detected in summer. With the aim to confirm the role of testosterone in these cells, the immunogold loca...

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ea0029p1054 | Male Reproduction | ICEECE2012

The high prevalence of testosterone deficiency in population of Polish men over 65 years with erectile dysfunction

Rabijewski M. , Papierska L. , Zgliczynski W.

About 810% of erectile dysfunction (ED) is caused by hormonal causes, but in men over 65 years testosterone deficiency due to aging seems to play important role. In population of Polish men with relative poor health status, the prevalence of testosterone deficiency in elderly men with ED is probably higher. We evaluated the prevalence of low and low-normal testosterone levels in men with ED in population of Polish men over 65 years.Material and met...

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ea0029p1055 | Male Reproduction | ICEECE2012

Production of nitric oxide and peroxynitrite by human spermatozoa and their possible role on sperm motility

Vignini A. , Buldreghini E. , Nanetti L. , Raffaelli F. , Paggi F. , Mazzanti L. , Salvolini E. , Balercia G.

Spermatozoa generate small amounts of O2°- and NO°. Under physiological condition these compounds exist at very low concentration and the formation of peroxynitrite (ONOO−) appears likely. Peroxynitrite reacts rapidly with proteins, lipids and DNA. Moreover, tyrosine nitration is a widely used marker of peroxynitrite. The nitration of protein residues gives rise to 3-nitrotyrosine which represents a protein modification specific for ONOO− formation in ...

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ea0029p1056 | Male Reproduction | ICEECE2012

Two infertile patients with 45X/46XY mosaicism and structural rearrangements of chromosome Y present with different phenotypes

Ibarra Gasparini D. , Pelusi C. , Gambineri A. , Altieri P. , Scarano E. , Mazzanti L. , Pasquali R.

Introduction: Within the high prevalence of male infertility there is the rare 45,X/46,XY mosaicism, associated or not with hypogonadism. The phenotypes of this genetical disorder present with a wide variability ranging from Turner stigmata to apparent normal male.Case report: Two men who presented with azoospermia in the context of couple infertility were subsequently found to be affected by 45,X/46,XY mosaicism. Physical examination revealed a complete...

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ea0029p1057 | Male Reproduction | ICEECE2012

Abstract withdrawn....

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ea0029p1058 | Male Reproduction | ICEECE2012

Seminal vesicles ultrasound features in a cohort of infertility patients

Lotti F. , Corona G. , Colpi G. , Filimberti E. , Innocenti SDegli , Mancini M. , Baldi E. , Noci I. , Forti G. , Maggi M.

Introduction: Previous studies concerning ultrasound evaluation of the seminal vesicles (SV) have been performed on a limited series of subjects, taking into consideration few parameters, often only before ejaculation and without assessing sexual abstinence of the patients. The aim of this study is to evaluate the volume and the emptying characteristics of the SV and their possible correlations with scrotal and transrectal ultrasound features.Methods: Th...

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ea0029p1059 | Male Reproduction | ICEECE2012

Nitric oxide synthase and tyrosine nitration in idiopathic asthenozoospermia: an immunohistochemical study

Salvolini E. , Buldreghini E. , Lucarini G. , Vignini A. , Di Primio R. , Balercia G.

It has been previously shown that human spermatozoa produce nitric oxide (NO), a short-lived free radical synthesised by three isoforms of NO synthase (NOS): endothelial (eNOS), neuronal (nNOS), and inducible (iNOS), which are responsible for the conversion of L-arginine to L-citrulline and NO. Spermatozoa of fertile men express both eNOS and nNOS. The specific roles of NOS isoforms in gametes are not well understood, even if iNOS seems to ...

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ea0029p1060 | Male Reproduction | ICEECE2012

Testosterone replacement therapy: A comparison of testosterone mixed esters, extended release testosterone implants and testosterone undecanoate depot

Conaglen Helen M. , Yarndley Tania , Kamp Jozef , Paul Ryan G , Nirmalaraj Kingsley , Elston Marianne S , Conaglen John V

Introduction: Male hypogonadism is one of the most common endocrine disorders affecting 5.6% of men aged between 3079 years. Testosterone deficiency (TD) is an independent risk factor for multiple cardiovascular risk factors including obesity, diabetes mellitus, hypertension, dyslipidaemia and endothelial dysfunction. The aims of testosterone replacement treatment (TRT) in hypogonadism are to improve or ameliorate the clinical features and to restore the serum T to physi...

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ea0029p1061 | Male Reproduction | ICEECE2012

Abstract withdrawn...

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ea0029p1062 | Male Reproduction | ICEECE2012

Reduced fatigue and improved sexual function in hypogonadal men treated with TESTIM in routine daily practice

Behre H. , Tolle A. , Gedamke M.

Introduction: Hypogonadal men frequently suffer from fatigue and reduced sexual function. International guidelines recommend testosterone replacement therapy (TRT) for symptomatic hypogonadal men. Our aim was to document testosterone changes and the impact of TRT with TESTIM on quality of life in routine daily practice.Methods: In this non-interventional-study (NIS) data from 117 hypogonadal patients from 43 office-based-study-sites were evaluated. Data ...

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ea0029p1063 | Male Reproduction | ICEECE2012

The mitochondrial citrate carrier regulates insulin secretion by human male gamete

Cappello A. , Guido C. , Santoro A. , Santoro M. , Amicis F. De , Montanaro D. , Marsico S. , Dolce V. , Aquila S.

Sperm energy management is poorly studied and understood. The present study provides biochemical and morphological evidence that ejaculated human sperm express the mitochondrial citrate carrier (CIC) which is restricted to the midpiece. CIC inhibition by the specific substrate analogue 1,2,3-benzenetricarboxylate (BTA) reduced cholesterol efflux, proteins tyrosine phosphorylation, P-AKT, P-P60src addressing to its functional role in sperm acquisition of fe...

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ea0029p1064 | Male Reproduction | ICEECE2012

Mild androgen insensitivity syndrome due to novel mutation in androgen receptor gene

Ivannikova E. , Dzeranova L. , Kurilo L. , Polyakov A. , Chernykh V. , Pigarova E.

We describe a clinical case of the patient with the syndrome of partial resistance to androgens. The patient, a man 25 years old, complained of a barren marriage. Lab and clinical findings included high gonadotropin and testosterone levels, hypogonadal appearance, coronal hypospadias, left-sided varicocele, oligoastenotheratozoospermia. The results of cytogenetic analysis showed a normal male karyotype (46, XY). Molecular genetic studies identified mutation in exon 1 of androg...

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ea0029p1065 | Male Reproduction | ICEECE2012

Semen quality in patients with newly diagnosed Hodgkin's disease

Peros K. , Bozek T. , Zmire J. , Colak B. , Stakor M. , Prkacin I. , Karakas A.

Objective: To analyse the semen quality in patients with newly diagnosed Hodgkins disease before starting the treatment.Materials and methods: We evaluated semen quality in 67 patients with Hodgkins disease who underwent sperm banking in our clinic over a 10-year period. Semen samples were collected by masturbation after 27 days of sexual abstinence. Age at banking, semen volume, sperm concentration, and total and progressive sperm moti...

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ea0029p1066 | Male Reproduction | ICEECE2012

Subcutaneous gonadotropin therapy for male infertility by hypogonadotropic hypogonadism

Moreno P. , Moreno M. Galvez , Exposito M. Alhambra , Sanchez I. Prior , Jimenez C. Munoz , Ortega R. Palomares , Lopez P. Benito

Objetive: Evaluate the efficacy of subcutaneous HCG with or without FSH in male infertility associated to hypogonadotropic hypogonadism (HH).Patients and methods: Descriptive study of patients with HH treated with HCG s.c. with or without FSH, for fertility objective (20042011). The Treatment was initiated with HCG 500 UI/72 h s.c., periodic monitoring of testosterone and semen analysis was realized, adjusting doses up to 2500 UI/72 h. If no answer...

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ea0029p1067 | Male Reproduction | ICEECE2012

Translation of EMAS sexual functions questionnaire from English to Lithuanian

Prakapiene E. , Matulevicius V. , Nikulina S. , Preiksa R. , Velickiene D. , Lasaite L. , Buteikis A. , Buteikiene A. , Adomaitiene V. , Sakalauskas R.

EMAS - SFQ is recently available instrument, validated and prooved valid in multicentral European study of more than 3000 eldery men (OConnor DB, Corona G & Forti G et al. 2008). This questionnaire assess male sexual functions rather than only erection disturbances.The aim of this study was to perform translation of EMAS - SFQ from English to Lithuanian for assessing elderly Lithuanian men suffering of prostate cancer. Permission for tra...

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ea0029p1068 | Male Reproduction | ICEECE2012

A surprising diagnosis in a man with high testosterone levels

Srbova L.

Introduction: Marked elevation of testosterone levels can be caused by tumors with hormonal hypersecretion. However, we should be aware of the relationship between thyroid and sexual hormones, including changes of SHBG.Case report: A 48-year-old man was recommended by an urologist to endocrinological examination due to high levels of total testosterone.CT scan of abdomen, pelvis and the pituitary did not show any abnormalities....

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ea0029p1069 | Male Reproduction | ICEECE2012

Recapture of the study on the reproductive function of Estonian, Latvian and Lithuanian Young Men after 8 years

Nikulina S. , Abramavicius S\. , Preiksa R. , Matulevicius V. , Adomaitiene V. , Linkevicius R. , Verkauskiene R. , Sabeckiene N. , Zilaitiene B. , Pundzius J. , Saukiene I.

In 20032004 year a study named The Reproductive Function of Estonian, Latvian and Lithuanian Young Men, 1825 year aged, was performed (QLK4-CT-1999-01422, NAS: QLK4-CT-2001-02911). Determination of semen quality, physical appearance and testicular size in relation to the level of reproductive hormones and environment, including life style factors was performed.The aim of our study was to recapture young men, who had participate in the study 7...

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15th International & 14th European Congress of Endocrinology

BiosciAbstracts