ICEECE2012 Oral Communications Diabetes Basic (6 abstracts)
Faculty of Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, Brazil.
Introduction: MicroRNAs (miRNA) are noncoding RNAs that play a central role in governing a variety of physiological and pathological processes, and there are few studies on miR involvement in diabetes mellitus. There are three principal types of diabetes, i.e., type 1 (T1DM), type 2 (T2DM) and gestational diabetes mellitus (GDM). In this study we performed a meta-analysis of the miRNA expression profiling of peripheral blood mononuclear cells (PBMC) from T1DM, T2DM and GDM patients by using the microarray technology.
Methods/Design: Agilent human miRNA microarray kits (v3) 8×15 K were used to perform hybridizations of samples from T1DM (n=4), T2DM (n=4) or GDM (n=5) patients that were quantified and analyzed using GeneSpring GX11 pack (Agilent). ANOVA (P<0.05) with Benjamini-Hochberg FDR multiple testing correction was performed. Finally, miRNAs exhibiting fold change ≥2.0 were considered for the study.
Results: Ten miRNA were differentially expressed in the 3 types of diabetes: hsa-miR-328, hsa-miR-181b, hsa-miR-1306, hsa-miR-181c, hsa-miR-1275, hsa-let-7b*, hsa-miR-939, hsa-miR-623, hsa-miR-595, and hsa-miR-1268. None of these miRNAs were previously described in association with any type of diabetes. Interestingly, two members of miR-181 family contribute to T cell tolerance, and this miRNA family has been involved in the inhibition of IL-2 expression.
Conclusion: Considering that autoimmunity is characteristic of T1DM, considering the similarities of mRNA expression profiles between T1DM, T2DM and GDM, and considering that miR-181 family modulates some cytokine expression, these results indicate that miRNA may be used as marker for diabetes. Financial support: FAPESP, CNPq.
Declaration of interest: The authors declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research project.
Funding: This work was supported, however funding details are unavailable.