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15th European Congress of Endocrinology

Poster Presentations

Pituitary–Basic (<emphasis role="italic">Generously supported by IPSEN</emphasis>)

ea0032p823 | Pituitary–Basic (<emphasis role="italic">Generously supported by IPSEN</emphasis>) | ECE2013

Gene expression profiling of familial and sporadic pituitary adenomas

Barry Sayka , Gadaleta Emanuela , Chelala Claude , Korbonits Marta

Background: Familial isolated pituitary adenoma (FIPA) is recently identified autosomal dominant condition with incomplete penetrance. Heterozygote mutations have been identified in the aryl-hydrocarbon receptor interacting protein (AIP) gene in 20–30% of FIPA families. AIP mutation positive patients have distinct phenotype: the disease is occurring at a younger age and have more aggressive tumours.Aims: The aim of this study was to perform comparat...

ea0032p824 | Pituitary–Basic (<emphasis role="italic">Generously supported by IPSEN</emphasis>) | ECE2013

Role of filamin-A in the regulation of SST2 receptor localization and signalling in tumoral GH-secreting cells

Giardino Elena , Peverelli Erika , Vitali Eleonora , Cambiaghi Valeria , Lania Andrea G , Peccoz Paolo Beck , Mantovani Giovanna , Spada Anna

Somatostatin (SS) binds to different SS receptors (SSTR1–5) and SS analogues are the first choice medical treatment of GH-secreting pituitary adenomas (GH-omas). A subset of patients is resistant to SS, although the mechanisms involved in SS resistance are not fully understood. Recent studies identified specific protein–protein interactions as determinant in the regulation of receptor anchoring and signalling. Filamin A (FLNA) is a widely expressed cytoskeleton prote...

ea0032p825 | Pituitary–Basic (<emphasis role="italic">Generously supported by IPSEN</emphasis>) | ECE2013

Drosophila melanogaster as a model organism to study aryl hydrocarbon receptor interacting protein gene function

Aflorei Elena Daniela , Chen Chenghao , Klapholz Benjamin , Grossman Ashley B , Tapon Nicolas , Brown Nicholas H , Stanewsky Ralf , Korbonits Marta

Introduction: Loss-of-function mutations in AIP are associated with familial isolated pituitary adenoma, often leading to gigantism due to invasive GH-secreting pituitary adenomas. One challenging problem in the management of patients carrying a missense AIP variant is to determine whether the missense variant is a disease-causing mutation or not. As most of the molecular mechanisms involved in the control of growth and the cell cycle are well-conserved, we p...

ea0032p826 | Pituitary–Basic (<emphasis role="italic">Generously supported by IPSEN</emphasis>) | ECE2013

The role of GLI1 in pituitary tumor formation and pituitary cell survival

Lampichler Katharina , Ferrer Patricio , Vila Greisa , Luger Anton , Knosp Engelbert , Wagner Ludwig , Baumgartner-Parzer Sabina

The transcription factor and proto-oncogene GLI1 is the terminal mediator of the Hedgehog signalling pathway which is involved in developmental processes, stem cell maintenance and cell proliferation. Although the pathway is mainly active during embryogenesis and tissue repair, it is frequently reactivated in several cancer types.Based on these findings we investigated the potential role of GLI1 in the pathogenesis of pituitary adenomas. GLI1 expression ...

ea0032p827 | Pituitary–Basic (<emphasis role="italic">Generously supported by IPSEN</emphasis>) | ECE2013

cAMP exerts proliferative and anti-proliferative effects in pituitary cells of different types by activating both cAMP-dependent protein kinase A and exchange proteins directly activated by cAMP

Vitali Eleonora , Peverelli Erika , Mantovani Giovanna , Giardino Elena , Lania Andrea G. , Beck-Peccoz Paolo , Spada Anna

cAMP is implicated in the inhibition or stimulation of proliferation depending on cell type. The activation of cAMP–PKA pathway generates proliferative signals in GH-secreting adenomas whereas this effect is not present, or even opposite, in non-functioning pituitary cells (NFPA). Although cAMP effects were initially attributed to PKA activation, recently the discovery of two cAMP-activated guanine nucleotide exchange factors (Epac1,2) was proposed as a novel mechanism fo...

ea0032p828 | Pituitary–Basic (<emphasis role="italic">Generously supported by IPSEN</emphasis>) | ECE2013

Expression of somatostatin receptors, SSTR2A and SSTR5, in 108 pituitary adenomas, using immunohistochemical detection with specific MABs

Chinezu Laura , Vasiljevic Alexandre , Jouanneau Emmanuel , Francois Patrick , Borda Angela , Raverot Gerald , Trouillas Jacqueline

Background: Medical treatment of pituitary adenomas with somatostatin analogs depends on tumor type and somatostatin receptor expression. Using polyclonal antibodies, their immunohistochemical (IHC) detection gave conflicting results. Therefore, we studied the IHC expression of SSTR2A and SSTR5 using two specific MABs in five types of pituitary adenomas.Methods: SSTR2A and SSTR5 expression was studied using two...

ea0032p829 | Pituitary–Basic (<emphasis role="italic">Generously supported by IPSEN</emphasis>) | ECE2013

mTOR mediated IGF1 proliferative effects on a rat pituitary GH/PRL secreting pituitary adenoma cell line

Bellio Mariaenrica , Mirabassi Cecilia , Gagliano Teresa , Uberti Ettoredegli , Chiara Zatelli Maria

IGF1 represents an important growth factor in pituitary physiology and pathology, also mediating the negative feed-back mechanisms on somatotroph axis. It has been previously demonstrated that IGF1 promotes cell viability in primary cultures of human non functioning pituitary adenomas, by a mechanisms involving, at least in part, mTOR signaling, which is involved in many pathways controlling proliferation. We aimed at investigating whether mTOR may mediate IGF1 effects also in...

ea0032p830 | Pituitary–Basic (<emphasis role="italic">Generously supported by IPSEN</emphasis>) | ECE2013

Mutations of SOX2 gene: a novel heterozygous mutation and impact on congenital hypopituitarism

Auriemma Renata S , Macchiaroli Annamaria , Kelberman Daniel , Faienza Maria F , Corona Rosalia , Mariano Iolanda , Giangiobbe Sara , Galdiero Mariano , Pivonello Rosario , Colao Annamaria , Gasperi Maurizio

Introduction: Anophthalmia/microphthalmia is a rare developmental craniofacial defect often associated to congenital hypopituitarism with GH deficiency and hypogonadism. SOX2 gene plays a key role in embryonic development regulation and heterozygous mutations of this gene, reported only in 14 patients to date, have been associated to anophthalmia/microphthalmia and congenital hypopituitarism in uo to 10% of cases. Therefore, the study of the SOX2 gene can be clinically useful ...

ea0032p831 | Pituitary–Basic (<emphasis role="italic">Generously supported by IPSEN</emphasis>) | ECE2013

Chracterizazion of somatostatin receptor expression in MENX-assoxciated pituitary adenomas: impact on therapy and imaging with somatostatin analogs

Lee Misu , Lupp Amelie , Gartner Florian , Castano Justo P , Schulz Stephan , Pellegata Natalia

Introduction: Somatostatin analogs (SSAs) are the first-line clinical treatment for patients with pituitary adenomas (PAs) but their efficacy is highly variable among patients. This could be due to a differential expression of somatostatin receptor subtypes (Ssts) among tumors, but this issue is still unresolved. Nonfunctioning pituitary adenomas (NFPAs), which mostly derive from gonadotroph cells, are among the tumors that poorly respond to SSAs. Rats carrying a germline loss...

ea0032p832 | Pituitary–Basic (<emphasis role="italic">Generously supported by IPSEN</emphasis>) | ECE2013

Clinical and morphological characteristics of dopamine agonist-resistant prolactinomas

Fedorova Natalia , Dzeranova Larisa , Pigarova Ekaterina , Abrosimov Alexander , Astaf'eva Liudmila , Rozhinskaya Liudmila

Introduction: The aim of our study was to investigate clinical and morphological features of dopamine agonist-resistant prolactinomas.Methods/design: The study included 51 patients (38 women, 13 men) with prolactin-secreting pituitary adenoma that is resistant to treatment with dopamine agonists, age 15–82 years.Results: Median of the serum PRL level distribution at the onset of the disease was 4500 mIU/l (3480, 140 300). The ...

ea0032p833 | Pituitary–Basic (<emphasis role="italic">Generously supported by IPSEN</emphasis>) | ECE2013

Expression of connexins 26, 32 and 43 mRNA in normal and pituitary adenomas

Nunes Bruno , dos Santos Faria Manuel , Fortunato Rodrigo , Eurico Nasciutti Luiz , Renner Urlich , Stalla Gunter K. , de Carvalho Denise Pires , Miranda-Alves Leandro

Connexins are a proteins critically involved in the formation of gap junction, which connect cells with each other and enable the exchange of small compounds and, besides having a role independently of gap junction formation. At least, in the anterior pituitary two cellular networks have already been identified, one of them is composed of the endocrine inactive folliculo-stellate cells and the second one is the GH-producing somatotroph cell network. It is supposed that gap jun...

ea0032p834 | Pituitary–Basic (<emphasis role="italic">Generously supported by IPSEN</emphasis>) | ECE2013

No correlation between somatostatine 2 receptor expression analyzed by RNA in-situ hybridization and real-time qRT-PCR in clinically non-functioning pituitary adenomas

Gabalec Filip , Kohout Ales , Laco Jan , Drastikova Monika , Beranek Martin , Netuka David , Masopust Vaclav , Cesak Tomas , Cap Jan

Objective: The aim of this study was to quantitatively estimate SSTR2 in clinically non-functioning pituitary adenomas (CNFAs) with use of RNA in-situ hybridization (ISH) and quantitative real-time RT-PCR and correlate the results of both methods.Methods: A standard histological and immunohistochemical examination was performed on the resected pituitary tumour. Afterwards in-situ hybridization for somatostatine 2 receptor (SSTR2) RNA wa...

ea0032p835 | Pituitary–Basic (<emphasis role="italic">Generously supported by IPSEN</emphasis>) | ECE2013

FSH and LH cells in normal-fed male rats after centrally applied ghrelin

Milosevic Verica , Ajdzanovic Vladimir , Nesic Dejan , Starcevic Vesna , Ristic Natasa , Rakocevic Rastko , Stevanovic Darko

Ghrelin, the endogenous ligand of GH secretagogue receptor type 1a, has emerged as pleiotropic modulator of diverse biological functions, including energy homeostasis and recently, reproduction. The effects of i.c.v. administered ghrelin on morphological characteristics of pituitary FSH- and LH-cells were examined in adult male Wistar rats. The animals were randomized in two groups: control and experimental group, each consisted of seven animals, and implanted with an i.c.v. c...

ea0032p836 | Pituitary–Basic (<emphasis role="italic">Generously supported by IPSEN</emphasis>) | ECE2013

PTTG and Ki-67 expression in pituitary adenomas

Golkowski Filip , Sokolowski Grzegorz , Wierzbicka-Tutka Iga , Baldys-Waligorska Agata , Adamek Dariusz , Hubalewska-Dydejczyk Alicja

Introduction: During clinical observation the behaviour of pituitary tumours is often unpredictable. The work was aimed at evaluating Ki-67 and pituitary tumour transforming gene (PTTG) indices in pituitary adenomas.Material and methods: The Ki-67 and PTTG indices were determined by immunochemistry in specimens excised from neurosurgically removed pituitary tumours. In fifty two examined patients (30 females and 22 males, mean age 50.5±15.4 years) w...

ea0032p837 | Pituitary–Basic (<emphasis role="italic">Generously supported by IPSEN</emphasis>) | ECE2013

Medical therapy of acromegaly

Palha Ana , Cortez Luisa , Sabino Teresa , Silva Nunes Jose , Afonso Antonio , Fonseca Fernando , Agapito Ana

Introduction: Medical therapy of acromegaly is indicated in patients who failed to achieve remission after surgery, with contraindication or refusal for surgical therapy or following radiotherapy in order to bridge the interval until complete remission.Objective: Evaluate the medical therapy – somatostatin analogs (SSA), dopamine agonist (DAs) and GH receptor-antagonist (Pegvisomant) – in acromegalic patients followed from 1988–2011.<p...

ea0032p838 | Pituitary–Basic (<emphasis role="italic">Generously supported by IPSEN</emphasis>) | ECE2013

Daidzein affects the stereological parameters of pituitary GH cell in orchidectomized adult rat

Trifunovic Svetlana , Ajdzanovic Vladimir , Filipovic Branko , Miler Marko , Ristic Natasa , Medigovic Ivana , Milosevic Verica

Daidzein, one of the main phytoestrogens of soy, is able to act as estrogen-like compounds due to its structural similarity with 17β-estradiol, which gives them the capacity to bind estrogen receptors and to induce hormone-like effects. Considering, that somatotropic system is sensitive to sex steroids, the aim of this study was to investigate the effects of daidzein on stereological and biochemical parameters of pituitary somatotropic (GH) cells in orchidectomized adult ...

ea0032p839 | Pituitary–Basic (<emphasis role="italic">Generously supported by IPSEN</emphasis>) | ECE2013

8-Prenylnaringenin decreases hormone expression in GH3 pituitary adenoma cells of the rat

Rupa Rosita , Voellger Benjamin , Kirches Elmar , Mawrin Christian , Firsching Raimund

Objective: 8-Prenylnaringenin (8-PN) is a phytoestrogen discovered in hops. GH3 pituitary adenoma cells of the rat show a variety of responses to phytoestrogens in vitro. This in vitro study aims to analyze changes in hormone expression in GH3 cells treated with 8-PN.Methods: GH3 cells were incubated with 0.5–50 μM 8-PN for 4 h. Medium and ethanol served as controls. Changes in expression of growth hormone (GH) and prolactin (...