Searchable abstracts of presentations at key conferences in endocrinology
Previous issue | Volume 35 | ECE2014 | Next issue

16th European Congress of Endocrinology

Oral Communications

Pituitary Basic

ea0035oc12.1 | Pituitary Basic | ECE2014

Human AIP gene rescue lethality in a Drosophila melanogaster knockout model of AIP orthologue

Aflorei Elena Daniela , Chen Chenghao , Klapholz Benjamin , Brown Nick , Stanewsky Ralf , Korbonits Marta

Introduction: Heterozygote loss-of-function mutations in AIP (Aryl hydrocarbon receptor interacting protein) predispose to young-onset pituitary adenomas. Homozygote murine knockout model of AIP leads to lethality. While the majority of the 75 published AIP mutations result in truncated or missing proteins, missense mutations are more difficult to characterise and to establish their pathogenic role. We have identified the orthologue of AIP, CG1847, in Drosophila melanogaster. ...

ea0035oc12.2 | Pituitary Basic | ECE2014

Differential in vitro response to octreotide and pasireotide in normal and tumoral primary pituitary cell cultures

Ibanez-Costa Alejandro , Gahete Manuel David , Jimenez-Reina Luis , Rivero-Cortes Esther , Lopez-Sanchez Laura Maria , Galvez Maria Angeles , de la Riva Andres , Benito-Lopez Pedro , Venegas-Moreno Eva , Angel Japon Miguel , Moreno Alberto , Garcia-Arnes Juan Antonio , Maraver-Selfa Silvia , Angel Arraez Miguel , Leal-Cerro Alfonso , Schmid Herbert A. , Tinahones Francisco J. , Soto-Moreno Alfonso , Castano Justo P. , Luque Raul M.

Somatostatin analogs (SSA) are a first-line treatment for pituitary adenomas (PA). Indeed, multi-receptor targeting SSA such as octreotide and pasireotide are being successfully used to control hormone secretion and/or tumor growth. Unfortunately, many PA escape from SSA-therapy, which could be related to somatostatin receptor (sst) presence, abundance, availability and/or signaling. In order to better define the molecular/cellular features associated to octreotide and pasireo...

ea0035oc12.3 | Pituitary Basic | ECE2014

Detection of pituitary antibodies by immunofluorescence: Methodological approach and results in patients with pituitary diseases

Ricciuti Adriana , De Remigis Alessandra , Landek-Salgado Melissa A. , De Vincentiis Ludovica , Guaraldi Federica , Lupi Isabella , Iwama Shintaro , Wand Gary S. , Salvatori Roberto , Caturegli Patrizio

Pituitary antibodies have been measured mainly to identify patients whose disease is caused or sustained by pituitary-specific autoimmunity. Although reported in over 100 publications, this antibody test has variable results and limited clinical utility. Our goals were to: identify major sources of variability; test them experimentally for establishing an optimized immunofluorescence protocol; assess prevalence and significance of pituitary antibodies in patients with pituitar...

ea0035oc12.4 | Pituitary Basic | ECE2014

Identification and characterization of pituitary adenoma stem-like cells in human non functioning pituitary adenomas

Giardino Elena , Peverelli Erika , Treppiedi Donatella , Belicchi Marzia , Meregalli Mirella , Spada Anna , Torrente Yvan , Mantovani Giovanna

Several studies support the existence of multipotent stem/progenitor cells in the adult pituitary, whereas the presence of stem cell in pituitary tumors and their role in pituitary tumorigenesis are still on debate. Aim of this study was to identify and characterize stem-like cells in non-functioning pituitary adenomas (NFPAs). To this purpose primary cell cultures from 25 NFPAs were grown in culture conditions that favored stem cell growth (EGF, bFGF and B27 containing medium...

ea0035oc12.5 | Pituitary Basic | ECE2014

Does hypercortisolism of Cushing’s syndrome affect telomere length?

Aulinas Anna , Ramirez Maria Jose , Barahona Maria Jose , Valassi Elena , Resmini Eugenia , Mato Eugenia , Santos Alicia , Crespo Iris , Bell Olga , Surralles Jordi , Webb Susan

Introduction: Hypercortisolism in CushingÂ’s syndrome (CS) determines increased mortality and morbidity. Hypercortisolism is also present in chronic depressive disorders and stress, where telomere length (TL) is shorter than in controls. We hypothesized that telomere shortening may occur and contribute to premature morbidity in CS.Aim: Investigate TL in CS compared to matched controls, and longitudinally in a subset of CS patients evaluated both with...