Searchable abstracts of presentations at key conferences in endocrinology
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16th European Congress of Endocrinology

Poster Presentations

Steroid metabolism and action

ea0035p946 | Steroid metabolism and action | ECE2014

Measurement of testosterone, androstenedione and dehydroepiandrosterone (DHEA) serum levels using isotope-dilution liquid-chromatography tandem mass spectrometry (ID-LC-MS/MS)

Buttler Rahel M , Martens Frans , Ackermans Mariette T , Blankenstein Marinus A , Heijboer Annemieke C

The adrenal and gonadal androgens testosterone, androstenedione and dehydroepiandrosterone (DHEA) play an important role in sexual development and fertility as well as in several other processes. We developed a method to assess serum testosterone, androstenedione and DHEA levels in one run using isotope-dilution liquid-chromatography tandem mass spectrometry (ID-LC-MS/MS). Sample preparation consisted of addition of internal standards (13C3-testosterone, ...

ea0035p947 | Steroid metabolism and action | ECE2014

Vitamin D is associated with androgen synthesis in human testicular cells

Hofer Daniela , Munzker Julia , Zachhuber Verena , Stiegler Philipp , Hutz Karla , Zigeuner Richard , Pieber Thomas , Muller Helmut , Obermayer-Pietsch Barbara

Introduction: Testosterone production in male testicular cells is mainly stimulated by LH, secreted by the pituitary. Recent findings have shown significant clinical associations of vitamin D seasonal rhythms and androgens in men (Wehr et al. 2010, Nimptsch et al. 2012). We aimed to elucidate associations of vitamin D and androgen synthesis on a cellular level.Methods: Human primary testicular cells, isolated from testes of braindead donors, and a cell l...

ea0035p948 | Steroid metabolism and action | ECE2014

Changes in serum steroid concentrations in relation to fat distribution and insulin resistance in women with polycystic ovarian syndrome

Ibanichuka Jacqueline , Vincent Royce P , Ghataore Lea , Taylor Norman F , Millward Ann , Pinkney Jonathan H , Stenhouse Elizabeth

Background: Polycystic ovarian syndrome (PCOS) affects 5–10% of women of reproductive age. Hyperandrogenism and hyperinsulinaemia have been attributed to the pathophysiology of PCOS. The aim of this study is to evaluate the effect of fat distribution and insulin resistance on steroid metabolism in PCOS.Methods: The study recruited 20 PCOS and 20 matched controls. Fat distribution was assessed by waist circumference, skin fold measurements, and body ...

ea0035p949 | Steroid metabolism and action | ECE2014

The role of steroid hormones in the development of postpartum depression

Duskova Michaela , Mikesova Michaela , Jirak Roman , Hill Martin , Parizek Antonin , Starka Luboslav

Introduction: Postpartum depression affects 10–15% women after childbirth. There is no currently generally accepted theory about the causes and mechanisms of postpartum mental disorders. The principal hypothesis concerns the association with sudden changes in the production of hormones affecting the nervous system of the mother and, on the other hand, with the ability of receptor systems to adapt to these changes.Methods: We observed changes in ster...

ea0035p950 | Steroid metabolism and action | ECE2014

Tissue cortisol vs lipolysis in ICU patients

Ilias Ioannis , Vassiliadi Dimitra , Theodorakopoulou Maria , Nikitas Nikitas , Tzanela Marinela , Apollonatou Sofia , Tsagarakis Stylianos , Dimopoulou Ioanna

Introduction: The interplay of cortisol (F) and adipose tissue is complex and in many aspects is still obscure. Plasma F has been shown to be positively associated with lipolysis.Aim: To study in adipose tissue indices of lipolysis vs tissue F with microdialysis (MD).Subjects and methods: We studied 46 mechanically ventilated patients with a diagnosis of septic/non-septic shock, systemic inflammatory response syndrome or s...

ea0035p951 | Steroid metabolism and action | ECE2014

Testosterone stimulates glucose uptake in HepG2 liver cells

Kelly Daniel , Akhtar Samia , Bowskill Susannah , Smallwood Zoe , Jones Hugh

Introduction: Testosterone deficiency is associated with an increased risk of type-2 diabetes (T2D) in men. Physiological testosterone replacement (TRT) improves insulin resistance and glycaemic control in hypogonadal men with T2D. The mechanisms underlying these actions remain unknown, but may be due in part to an effect on the liver as a major metabolic organ involved in glucose regulation. We have previously shown in testicular feminised mice, which have low testosterone le...

ea0035p952 | Steroid metabolism and action | ECE2014

A hypercoagulable state associated with intravenous pulse methylprednisolone (MP) therapy in patients with Graves' orbitopathy

Rutkowska Beata , Miskiewicz Piotr , Trautsolt-Jeziorska Katarzyna , Pirko-Kotela Katarzyna , Milczarek-Banach Justyna , Wolczanska Natalia , Jelenska Magdalena , Glowczynska Renata , Bednarczuk Tomasz

Introduction: Chronic exposure to glucocorticoid excess, either endogenous or exogenous, is associated with a hypercoagulable state and a high thromboembolism risk. The underlying mechanism of this phenomenon is still unclear. Clinical studies have indicated various abnormalities of procoagulant, anti-coagulant and fibrynolytic factors, e.g.: alterations of clotting factors (F), plasminogen activator inhibitor (PAI-1), plasminogen, antithrombin. Moreover, other consequences of...

ea0035p953 | Steroid metabolism and action | ECE2014

Adrenal androgen secreting tumour in 39-year-old woman: Hormones and sexuality

Matulevicius Valentinas , Jureviciute Justina , Krasauskas Virgilijus , Matuleviciute Indre , Banisauskaite Ilona , Ciaplinskiene Lina , Verkauskiene Rasa , Urbanavicius Vaidotas

Introduction: Androgen secreting adrenal tumours are rare and sometimes malignant.We report hormonal and sexual changes in 39-year-old woman with androgen secreting adrenal tumour completely healed by resection of the tumour.Case report: Androgen secreting adrenal tumour that manifested in 39-year-old woman with suddenly appeared amenorrhea, weight gain, hirsutism, epileptic seizures and elevated DHEA-S and testosterone is describe...

ea0035p954 | Steroid metabolism and action | ECE2014

Estradiol and its metabolite 2-methoxyestradiol modulate the growth of HECa10 line acting predominantly in the opposite way

Niedziela Agata , Melen-Mucha Gabriela

Introduction: The HECa10 line was derived from the specialized endothelial cells typically occurring in lymphoid tissues and belonging to high endothelial vessels (HEVs). Recent studies have shown that HEVs are also present within the tumor microenvironment, what unlike to other blood vessels, can be associated with favorable prognosis of cancer patients. Estradiol (E2) and its metabolite 2-methoxyestradiol (2ME2) are known as the important modulators of cardiovascular system ...

ea0035p955 | Steroid metabolism and action | ECE2014

Testosterone supplementation and sexual function: a meta-analysis study

Corona Giovanni , Isidori Andrea M , Buvat Jaques , Aversa Antonio , Ratrelli Giulia , Hackett Geoff , Rochira Vincenzo , Lenzi Andrea , Maggi Mario

Introduction: The role of testosterone supplementation (TS) as a possible treatment for male sexual dysfunction remains questionable. The aim of the present study is to meta-analyse data evaluating the effects of TS on male sexual function and its therapeutic synergism with the use of phosphodiesterase type 5 (PDE5i).Methods: An extensive Medline Embase and Cochrane search was performed including the following words: ‘testosterone’, ‘erect...

ea0035p956 | Steroid metabolism and action | ECE2014

Profiling of neuroactive steroids, their precursors and metabolites in patients suffering from multiple sclerosis

Kancheva Radmila , Hill Martin , Kancheva Lyudmila , Velikova Marta , Pavelcova Miluse , Starka Luboslav , Havrdova Eva

Some steroids modulate the permeability of ionotropic receptors on cell membranes and therefore may activate or inhibit neuronal activity depending on the steroid structure. These neuroactive steroids (NAS) exert neuroprotective effects like the neuronal remyelination.Multiple sclerosis (MS) is the most common cause of neurological disability in young adults. Therefore, the authors have followed the neuroprotective, GABAergic, glycinergic, glutamatergic ...

ea0035p957 | Steroid metabolism and action | ECE2014

Role of clinical risk factors and polymorphisms in glucocorticoid receptor gene in the determining the risk of developing new-onset diabetes after kidney transplantation

Michetti Grazia , Trementino Laura , Marcelli Giorgia , Apolloni Gloria , Taruscia Domenica , Frasca Giovanni Maria , Boscaro Marco , Faloia Emanuela , Arnaldi Giorgio

Introduction: New onset diabetes after transplantation (NODAT) is a recognized metabolic complication of kidney transplantation: its rates at 12 months after transplantation is between 20 and 50% for kidney recipients and it is associated with increased risks of graft rejection, infection, cardiovascular disease and death. Transplant-specific risk factors for NODAT,such as corticosteroids and calcineurin inhibitors, play a dominant role in its pathogenesis. Furthermore polymor...