Searchable abstracts of presentations at key conferences in endocrinology
Endocrine Abstracts (2015) 37 EP853 | DOI: 10.1530/endoabs.37.EP853

ECE2015 Eposter Presentations Thyroid cancer (90 abstracts)

Detection of somatic oncogene alterations in FNA samples of cold nodules and 3 years follow-up of patients in Hungary

Bálint Tobiás 1, , Bernadett Balla 1, , P János Kósa 1, , István Takács 1 , Zsolt Nagy 1 , Péter Horváth 1 , Balázs Járay 2 , Eszter Székely 2 , Roland Istók 2 , Tamás Székely 2 & Péter Lakatos 1


11st Department of Internal Medicine, Semmelweis University, Budapest, Hungary; 22nd Department of Pathology, Semmelweis University, Budapest, Hungary; 3Pentacore Laboratory, Budapest, Hungary.

Cold nodules are one of the most common findings on scintigraphic examinations of the thyroid gland. About 5–10% of these nodules turn out to be histologically malignant. Our aim was to examine some somatogenetic alterations associated with thyroid cancer in FNA samples of the thyroid. These alterations included single nucleotide mutations (BRAF, HRAS, NRAS, KRAS) and genetic translocations (RET/PTC1, RET/PTC3, PAX8ex7/PPARgamma, PAX8ex9/PPARgamma). The SNPs were tested by real-time PCR with fluorescence melting curve analysis and the rearrangements were detected by Taqman probe-based quantitative real-time PCR. We have analysed 779 consecutive FNA samples and followed up the patients 3 years long. In the examined 779 samples, we found different genetic alterations (39 BRAF, 23 NRAS, 9 HRAS, 1 KRAS mutations and 1 RET/PTC3 rearrangement). After 1 year follow-up by histology, 52 cases (6.8%) were classified as malignant, from which we identified genetic alterations only in 40 (5.1%). (specificity 93.3%, sensitivity 46.2%, negative predictive value 96.0%, positive predictive value 32.9%) In two years follow-up group (n=504) by histology, 30 cases (6.0%) were classified as malignant, from which we identified genetic alterations in 26 (5.2%). In three years follow-up group (n=250) by histology, 13 cases (5.2%) were classified as malignant, from which we found genetic alterations in 14 (5.6%). No PAX8/PPARgamma rearrangements were demonstrated in the 779 samples. These data are not in complete accordance with published information. This fact might be due to several factors including the differences in iodine supply in different geographical areas.

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