Searchable abstracts of presentations at key conferences in endocrinology
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18th European Congress of Endocrinology

Guided Posters

Receptors & Signalling

ea0041gp169 | Receptors & Signalling | ECE2016

Chemotherapy resistance and metastasis-promoting effects of thyroid hormone in hepatocarcinoma cells are mediated by suppression of FoxO1 and Bim pathway

Chi Hsiang-Cheng , Lin Kwang-Huei

Hepatocellular carcinoma (HCC) is the third leading cause of cancer-related death worldwide, and systemic chemotherapy is the major treatment strategy for late-stage HCC patients. Poor prognosis following chemotherapy is the general outcome owing to recurrent resistance. Recent studies have suggested that in addition to cytotoxic effects on tumor cells, chemotherapy can induce an alternative cascade that supports tumor growth and metastasis. In the present investigation, we sh...

ea0041gp170 | Receptors & Signalling | ECE2016

Altered exression of circadian clock genes in polyglandular autoimmune syndrome type III

Angelousi Anna , Nasiri-Ansari Narjes , Spilioti Eliana , Kalotychou Vasiliki , Chrousos George , Kaltsas Gregory , Kassi Eva

Introduction: CLOCK system is a highly conserved, ubiquitous molecular ‘clock’ which creates internal circadian rhythmicity under the influence of light/dark information. CLOCK system is regulated by the coordinated activation/inactivation of several transcription factors, including the CLOCK, the BMAL1 and other essential regulators, such as the Pers, Crys and RORs. The present study aimed to evaluate the circadian rhythm of clock-related genes expressed in patients...

ea0041gp171 | Receptors & Signalling | ECE2016

Zebrafish as a new model to study in vivo the functional consequences of human THRA variants

Marelli Federica , Carra Silvia , Rurale Giuditta , Cotelli Franco , Persani Luca

Introduction: Since 2012, different heterozygous mutations in the THRA gene have been described in patients with resistance to thyroid hormone alpha (RTHα). The associating symptoms are reminiscent of untreated congenital hypothyroidism (growth retardation, psycho-neuromotor disorders, delayed bone development and bradycardia) but with raised T3/T4 ratio and normal TSH levels. All genetic abnormalities act in a dominant negative (DN) manner against functional rec...

ea0041gp172 | Receptors & Signalling | ECE2016

Increased 17beta-hydroxysteroid dehydrogenase type 1 mRNA level is correlated with poor prognosis in endometrial cancer

Cornel Karlijn , Krakstad Camilla , Jori Balazs , Bongers Marlies , Kruitwagen Roy , Salvesen Helga , Romano Andrea

Endometrial cancer (EC) is estrogen dependent and several enzymes control the local availability of these steroids by activating (i.e. aromatase, sulfatase, 17beta-hydroxysteroid dehydrogenase type 1 – HSD17B1) or deactivating estrogens (sulfotransferase, 17beta-hydroxysteroid dehydrogenase type 2 – HSD17B2). Imbalances in these enzymes are implied in EC development.In the present study, it is evaluated whether imbalances in these enzymes are a...

ea0041gp173 | Receptors & Signalling | ECE2016

The changing ‘steroid metabolome’ across the spectrum of non-alcoholic fatty liver disease

Moolla Ahmad , Amin Amin , Hughes Bev , Arlt Wiebke , Hassan-Smith Zaki , Armstrong Matt , Newsome Philip , Shah Tahir , Van Gaal Luc , Verrijken An , Francque Sven , Biehl Michael , Tomlinson Jeremy

Introduction: Dysregulated glucocorticoid (GC) metabolism has been implicated in the pathogenesis of non-alcoholic fatty liver disease (NAFLD). NAFLD extends from simple steatosis, to inflammation (steatohepatitis/NASH), fibrosis and consequent cirrhosis. It is often regarded as the hepatic manifestation of the metabolic syndrome and is independently associated with increased liver and cardiovascular mortality. Changes in GC metabolism have thus far been described in small num...

ea0041gp174 | Receptors & Signalling | ECE2016

5β-reductase (AKR1D1) is a regulator of glucose homeostasis in human hepatocytes and development of model systems to define its role in metabolic liver disease

Nikolaos Nikolaou , Dunford James , Green Charlotte , Lee Wenhwa , Lim Reina , Gathercole Laura , McKeating Jane , Oppermann Udo , Hodson Leanne , Tomlinson Jeremy

Non-alcoholic fatty liver disease is the hepatic manifestation of the global epidemic of metabolic disease. Steroid hormones, including glucocorticoids and sex steroids, regulate metabolic phenotype, and in addition, bile acids have recently been identified as potent metabolic regulators. 5β-reductase (AKR1D1) is predominantly expressed in the liver and is a crucial regulator of steroid hormone clearance as well as bile acid synthesis. Its role in pathogenesis of metaboli...

ea0041gp175 | Receptors & Signalling | ECE2016

Differential roles of estrogen receptors (ERα and ERβ) in seminiferous epithelium: in vivo agonist studies in adult rats

Dumasia Kushaan , Kumar Anita , Balasinor Nafisa

Estrogen, through its receptors, plays an important role in regulation of spermatogenesis. The importance of estrogen is highlighted with the reports that environmental estrogens have deleterious effects on spermatogenesis and have been associated with declining sperm counts in men. Although estrogen receptors (ERα and ERβ) have been localised in the seminiferous epithelium; their precise roles are yet unclear as in vivo estradiol treatment would signal thro...

ea0041gp176 | Receptors & Signalling | ECE2016

Luteinizing hormone and human chorionic gonadotropin (hCG) action on the same receptor results in different in vitro intracellular signaling in mouse primary Leydig cells

De Pascali Francesco , Riccetti Laura , Poti Francesco , Tagliavini Simonetta , Trenti Tommaso , Simoni Manuela , Casarini Livio

Luteinizing hormone (LH) and human chorionic gonadotropin (hCG) are two glycoprotein hormones regulating development and reproduction. Despite binding the same receptor (LHCGR), they elicit different intracellular signaling. In vitro non-equivalence of hCG and LH was previously demonstrated in human primary granulosa cells. The aim of this study is to compare the effects of LH and hCG in mouse primary Leydig cells in vitro, naturally expressing the murine rec...

ea0041gp177 | Receptors & Signalling | ECE2016

Characterization of the signaling and testicular functions of bone morphogenetic protein 8

Wu Fang-Ju , Luo Ching-Wei

Previous studies have indicated that targeted depletion of Bmp8 in mice can cause the reduction of germ cells in the postnatal testis and this further leads to male infertility, indicative of its importance in the maintenance of spermatogenesis. However, except the genetic impacts, no direct signaling pathway of BMP8 in cells has been interpreted in vitro due to the lack of functional BMP8 proteins. To solve this, we firstly generated different constructs for...

ea0041gp178 | Receptors & Signalling | ECE2016

Salivary testosterone to androstenedione (T/A4) ratio is significantly higher in PCOS patients and accompanied with an adverse metabolic phenotype

Muenzker Julia , Lindheim Lisa , Adaway Jo , Lerchbaum Elisabeth , Pieber Thomas , Keevil Brian , Obermayer-Pietsch Barbara

Background: The aim of this study was to evaluate salivary androgen measurements via liquid chromatography-mass spectrometry as a non-invasive tool for the diagnosis of polycystic ovary syndrome (PCOS) and the assessment of metabolic health.Methods: Saliva and serum samples of 274 patients with PCOS fulfilling Rotterdam criteria and 51 BMI-matched, premenopausal, healthy controls were analysed for steroid hormones using liquid chromatography/mass spectro...