Searchable abstracts of presentations at key conferences in endocrinology
Endocrine Abstracts (2016) 41 EP970 | DOI: 10.1530/endoabs.41.EP970

ECE2016 Eposter Presentations Thyroid (non-cancer) (120 abstracts)

The immune status peculiarities depending on the level of thyroid peroxidase antibodies in Graves’ disease

Sergey Dogadin 1, , Margarita Dudina 1, , Andrey Savchenko 2 & Valentina Matsynina 3


1Krasnoyarsk State Medical University named after Prof. V. F. Voino-Yasenetsky, Krasnoyarsk, Krasnoyarsk region, Russia; 2Institute for Medical Problems of the North, Siberian Division, Russian Academy of Medical Sciences, Krasnoyarsk, Krasnoyarsk region, Russia; 3Krasnoyarsk regional clinical hospital, Krasnoyarsk, Krasnoyarsk region, Russia.


It is known that thyroid peroxidase is expressed on the apical surface of thyrocytes, where catalyze the iodination of thyroglobulin and may, also, be a surface-cell antigen, which involves in the complement-dependent cytotoxicity reactions. Objective: to study the immune status indicies depending on thyroid peroxidase (anti-TPO) antibodies in patients with Graves’ disease (GD). Materials and methods. The study included 35 women aged from 18 to 55 years, mean age of 39.1±7.2, with manifestations of GD, before antithyroid therapy. Concentrations of thyroid hormones were measured by RIA. Anti-TPO concentrations was assessed by ELISA. The analysis of lymphocytes subset pattern were performed by indirect immunofluorescence using the FITC-marked monoclonal antibodies to CD3, CD4, CD8, CD16, CD19, HLA-DR. The Ig A, M, G concentrations were determined by ELISA. The CD4+/CD8+T cell ratio and the relative synthesis of IgA (IgA /CD19+), IgM (Ig M/CD19+) and IgG (IgG/CD19+) were also calculated. Results. The patients with more than 100 IU/L anti-TPO level were characterized by an increase in absolute lymphocytes, relative and absolute number of CD19+ and HLA-DR+cells, CD3+ and CD8+cells, compared to the control range and patients with anti-TPO less than 100 IU/L. In GD patients with at-TPO level more than 100 IU/l the leuko-T- and leuko-B-cell ratios reduction, along with the IgA and IgM relative synthesis decreasing were observed. The only difference of patients with anti-TPO less than 100 IU/l was the increasing of absolute lymphocytes number (P<0.05). But in this group relationship anti-TPO with relative and absolute content of B-lymphocytes (r=+0.50, P=0.026 and r=+0.47, P=0.39, respectively), and leuko-T-cell ratio (r=−0.58, P=0.007) have been appeared. Conclusion. The most significant changes of immunological indices in GD were found in patients with more than 100 IU/L anti-TPO level. In the case of anti-TPO level less than 100 IU/l the immunopathogenesis of GD is characterized by the appearance of positive relationships anti-TPO with B-, and negative with T-cell immunity indicies.

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