Searchable abstracts of presentations at key conferences in endocrinology
Previous issue | Volume 59 | SFEBES2018 | Next issue

Society for Endocrinology BES 2018

Glasgow, UK
19 Nov 2018 - 21 Nov 2018

Card image cap
The Society for Endocrinology BES will take place 19-21 Nov 2018 in Glasgow. Come and exchange knowledge, share experiences and strengthen collaborations across our global community of endocrinologists.

Poster Presentations

Obesity & metabolism

ea0059p153 | Obesity & metabolism | SFEBES2018

Subcutaneous adipose tissue from patients with Idiopathic Intracranial Hypertension exhibits metabolically distinctive characteristics

Westgate Connar , Markey Keira , Ludwig Christian , Singhal Rishi , Lavery Gareth , Sinclair Alexandra

Idiopathic intracranial hypertension (IIH) is characterised by raised intracranial pressure (ICP) and papilloedema, diagnosed primarily in obese women of reproductive age, with the incidence rising with the global epidemic of obesity. Weight-loss lowers ICP and treats IIH. No mechanism explains the link between obesity and raised ICP. We hypothesise that adipose tissue from IIH patients has a metabolically distinct profile that contributes to raised ICP. Our previous data demo...

ea0059p154 | Obesity & metabolism | SFEBES2018

Dominant-negative mutations in PPAR alpha are present in unselected human populations and have a metabolic signature

Melvin Audrey , Lam Brian , Langenberg Claudia , Agostini Maura , Schoenmakers Erik , Luan Jian'an , Rainbow Kara , Yeo Giles S , Wareham Nick , Savage David B , Chatterjee Krishna , O'Rahilly Stephen

The study of humans carrying dominant negative mutations in PPAR gamma has contributed significantly to our understanding of its role in human physiology. To date, comparable studies of PPAR alpha have not been reported. Using a pooled approach, we undertook exon sequencing of PPARA in 11,848 adult participants of the Fenland study, a population-based cohort study with detailed metabolic phenotyping. Twenty-nine PPARA missense variants were detected (allelic ...

ea0059p155 | Obesity & metabolism | SFEBES2018

Identity and cell fate of Ngn3-expressing population in small intestinal organoids

Pedro Patricia Fonseca , Tsakmaki Anastasia , Bewick Gavin

The intestinal epithelium (IE) is populated by different cell types each with a unique set of functions. Each cell type is derived from a common progenitor, the stem cell. The hierarchy of epithelial cell fate is transcriptionally regulated for example, Notch signalling defines secretory versus absorptive destiny. Peptide hormone producing enteroendocrine (EE) cells are scattered throughout the epithelium where they integrate complex nutrient signals and respond by promoting m...

ea0059p156 | Obesity & metabolism | SFEBES2018

Randomised trial of empagliflozin versus metformin in polycystic ovary syndrome

Javed Zeeshan , Papageorgiou Maria , Kilpatrick Eric , Abbas Jehangir , Khan Amer , Rigby Alan , Atkin Stephen , Sathyapalan Thozhukat

Background: Empagliflozin is a sodium-glucose-cotransporter-2 that improves cardiovascular risk and weight loss in patients with type 2 diabetes. Polycystic ovary syndrome (PCOS) is associated with obesity and increased cardiovascular risk; therefore, empagliflozin may be of benefit in PCOS.Methods: A randomised, open-label study in 40 overweight and obese women with PCOS treated with either empagliflozin 25 mg or metformin 1500mg daily for 12 weeks....

ea0059p157 | Obesity & metabolism | SFEBES2018

The impact of lipopolysaccharide on mitochondrial efficiency in brown adipocytes

Omran Farah , McTernan Philip , Christian Mark

Background: The presence of brown adipose tissue (BAT) in adults offers an opportunity to examine inflammatory factors that may affect metabolic function in states of obesity. Gut-derived lipopolysaccharide (LPS), which is elevated in obesity, and initiates the innate immune response in white adipose tissue, has not been fully studied in BAT. The interactions between LPS, TLR4 and ß3-adrenergic receptors in BAT is unknown. ß3-adrenergic receptor ligands as CL 316,243...

ea0059p158 | Obesity & metabolism | SFEBES2018

Increased pro-inflammatory cytokine production in vitamin B12 deficient adipocytes

Samavat Jinous , Adaikalakoteswari Antonysunil , Boachie Joseph , Saravanan Ponnusamy

Vitamin B12 (B12) is an essential micronutrient required for optimal hematopoietic, neurologic and other several metabolic reactions. Longitudinal studies and animal models showed that low maternal vitamin B12 deficiency is associated with the maternal obesity, development of insulin resistance and metabolic syndrome phenotype suggesting the crucial role of B12 in adipose tissue function. Although the mechanisms underpinning metabolic disorders remain poorly defined, the patho...

ea0059p159 | Obesity & metabolism | SFEBES2018

Vitamin B12 deficiency leads to adipocyte dysfunction by enhancing triglyceride biosynthesis and impairing fatty-acid oxidation: a new protagonist in metabolic disease?

Samavat Jinous , Adaikalakoteswari Antonysunil , Boachie Joseph , Saravanan Ponnusamy

Vitamin B12 (B12) is an essential micronutrient required for two key metabolic reactions. Longitudinal studies and animal models showed that B12-deficiency during pregnancy is associated with the maternal obesity, development of insulin resistance and metabolic syndrome phenotype. Although the mechanisms underpinning low B12 and metabolic disorders remain poorly defined, it’s becoming increasingly clear that lipid dysregulation is associated with obesity and co-morbiditie...

ea0059p160 | Obesity & metabolism | SFEBES2018

Screening for Cushing’s syndrome in a tier 3 weight management service

Glyn Tessa , Ho May , Lambert Anthony Paul , Thomas Julia , King Rhodri , Douek Isabelle , Andrews Robert

There is limited evidence for the role of screening for Cushing’s syndrome (CS). Patients referred to the specialist medical weight management service at Musgrove Park Hospital have routinely been screened for CS with either an overnight dexamethasone suppression test (ODST) or two 24hour urinary free cortisol (UFC) if evidence of dysglycaemia. We retrospectively analysed the results of all patients referred to the service between 2013–2016. 794 patients were seen as...

ea0059p161 | Obesity & metabolism | SFEBES2018

B12 Receptors and transporters regulate the uptake and storage of vitamin B12 in hepatocytes

Boachie Joseph , Adaikalakoteswari Antonysunil , Samavat Jinus , Ilona Goljan , Saravanan Ponnusamy

Background: The liver stores 10 μg cobalamin per gram protein and able to hold 50% (1–1.5 mg) of body’s B12. Making up the bulk of the liver, hepatocytes express receptors regulating cellular uptake of B12 in the liver. However, relationship between circulating and intracellular B12 levels as well as regulation of hepatic uptake of B12 is unexplored.Objective: To assess the regulation of cellular uptake and storage of B12 in hepatocytes in...

ea0059p162 | Obesity & metabolism | SFEBES2018

The relationship between obstructive sleep apnoea and quality of life in women with polycystic ovary syndrome: a cross-sectional study

Kahal Hassan , Tahrani Abd , Kyrou Ioannis , Dimitriadis Georgios , Kimani Peter , Barber Thomas , Nicholls Matthew , Ali Asad , Weickert Martin , Randeva Harpal

Background: Obstructive sleep apnoea (OSA) and polycystic ovary syndrome (PCOS) are associated with significant comorbidities and commonly coexist. The primary aim of this study was to examine the relationship between OSA and quality of life (QoL) in women with PCOS.Study design: Observational cross-sectional study.Methods: Women with PCOS were recruited from a single secondary care centre in the UK. PCOS was diagnosed according to...

ea0059p163 | Obesity & metabolism | SFEBES2018

Metformin treatment fails to restore fatty acid oxidation in low vitamin B12 hepatocytes

Boachie Joseph , Antonysunil Adaikalakoteswari , Samavat Jinus , Saravanan Ponnusamy

Background: Metformin is utilized in clinical trials for treatment of non-alcoholic fatty liver disease (NAFLD) and obesity. Metformin increases AMP:ATP ratio activating AMP activated protein kinase-alpha (AMPKα), a mediator of fatty acid oxidation (FAO) in the liver. Meanwhile, prolonged oral metformin therapy in T2DM decreases vitamin B12(B12) in patients and evidence shows that low B12 dysregulates lipid levels. We therefore investigated whether low B12 impairs FAO ind...

ea0059p164 | Obesity & metabolism | SFEBES2018

Prevalence of adenovirus 36 infection and association with obesity and diabetes in the United Arab Emirates

Lessan Nader , Radha Saradalekshmi Koramannil , Barakat Maha T , Atkinsons Richard L

Background: Prevalence of obesity and diabetes has increased significantly in the UAE over the last 40 years. Adenovirus 36 (Adv36) infection has been associated with obesity in several studies across different ethnic populations, and usually is associated with improved glucose tolerance.Objective: 1) Identify the prevalence of Adv36 seropositivity among adults living in the UAE.2) Investigate the association of Adv36 infection wit...

ea0059p165 | Obesity & metabolism | SFEBES2018

Obesity and diabetes in adults with down syndrome: data from a large diabetes centre in the United Arab Emirates (UAE)

Fojas Esphie , Moriarty Maura , Lessan Nader , Barakat Maha T

Background: Down Syndrome (DS) is the most common chromosomal abnormality. Obesity and type 1 diabetes mellitus (T1DM) are known to be more prevalent in patients with DS than the general population. Available data on the prevalence of type 2 diabetes mellitus (T2DM) in DS is scant.Objectives: This study aims to investigate the prevalence of obesity and diabetes mellitus (DM) in adults with DS seen in Imperial College London Diabetes Centre (ICLDC) in the...

ea0059p166 | Obesity & metabolism | SFEBES2018

Evaluating non-face to face (NFTF) contacts for patients with Thyrotoxicosis

Mulla Kaenat , Razvi Yousuf , Srinivasan Balasubramanian Thiagarajan , Neill Katherine O'

Aim: To evaluate the feasibility of non-face to face (NFTF) contact in the follow-up for patients with thyrotoxicosis on carbimazole therapy.Background: While on carbimazole, achievement of a euthyroid state may involve multiple clinic appointments. We hypothesize by conducting these appointments in a NFTF setting, i.e. telephone consultation with trained nurse practitioner supported by an Endocrine consultant; a higher volume of consultations can occur ...

ea0059p167 | Obesity & metabolism | SFEBES2018

Male 5-beta reductase knockout mice have altered pancreatic islet morphology and hormone secretion

Harris Shelley , Gathercole Laura , Nikolaou Nikolaos , Ramracheya Reshma , Cox Roger , Forhead Alison , Tomlinson Jeremy

The enzyme 5β-reductase (AKR1D1) controls intra-cellular steroid hormone availability through hormone clearance. Additionally, it catalyses an essential step in bile acid (BA) synthesis. Disturbances in steroid hormones and BA metabolism have potent effects on metabolic health, therefore we hypothesize that AKR1D1 may play a role in metabolic homeostasis; the role of AKR1D1 in regulating glucose homeostasis and pancreatic function remains unexplored. We generated a global...

ea0059p168 | Obesity & metabolism | SFEBES2018

The impact of freeze dried broccoli extract to mitigate inflammation in human adipocytes through the mevalonate pathway

Murphy Alice , Azharian Sahar , Tripathi Gyanendra , Barker Guy , Chappell Michael , McTernan Philip G

Background: Delivery of nutrient excess in obesity can disrupt protein folding in the endoplasmic reticulum (ER) within adipose tissue; this activates the unfolded protein response (UPR) and contributes to type 2 diabetes mellitus (T2DM) risk. Thus, the aims of this study were to utilise freeze-dried broccoli extract (BE) as a nutrient to mitigate such cellular damage in human adipocytes, understand the relevance of associated pathways, and create a mathematical model of the U...

ea0059p169 | Obesity & metabolism | SFEBES2018

Angiopoietin-like ptotein 4 and 8 (ANGPTL4 and ANGPTL8) in human fetal liver are dysregulated by in utero exposure to maternal smoking

Talia Chiara , Filis Panagiotis , Soffientini Ugo , Lucendo-Villarin Baltasar , Douglas Alex , Hay David , Shaw Sophie , Iredale John , Swortwood Madeleine , Huestis Marilyn , Bellingham Michelle , Connolly Lisa , O'Shaughnessy Peter , Fowler Paul

Introduction: Angiopoietin-like proteins (ANGPTLs) are a family of 8 glycoproteins with pleiotropic effects in metabolism, angiogenesis, inflammation and cancer. ANGPTL3, 4 and 8 play major roles in regulating lipid levels, via inhibition of lipoprotein lipase. Increased serum ANGPTL3, 4, 8 levels are associated with obesity, diabetes, metabolic syndrome and fatty liver. Furthermore, cord blood ANGPTL8 is higher than in maternal serum, suggesting a role in fetal development an...

ea0059p170 | Obesity & metabolism | SFEBES2018

Effects of visfatin on brown adipose tissue energy regulation

Dimitriadis Georgios K , Adya Raghu , Tan Bee K , Jones Terence A , Menon Vinod S , Ramanjaneya Manjunath , Kaltsas Gregory , Miras Alexander D , Randeva Harpal S

The role of brown adipose tissue (BAT) in pathological states of energy homeostasis and impaired adipocyte function, such as obesity has been a major area of research interest in recent years. Herein, we sought to determine the direct effects of adipokines, visfatin and leptin on BAT thermogenesis. The effects of mouse recombinant visfatin, nicotinamide mononucleotide (NMN) and leptin with or without FK866 were studied on differentiated T37i cells. Treated cells were analyzed ...

ea0059p171 | Obesity & metabolism | SFEBES2018

Glucocorticoid-induced metabolic syndrome: establishing the role of AgRP

Sefton Charlotte , Harno Erika , Davies Alison , Allen Tiffany-Jayne , Wray Jonathan R , Coll Anthony P , White Anne

Glucocorticoid (Gc) excess, either from endogenous overproduction in disorders of the hypothalamic-pituitary-adrenal axis or exogenous medical therapy, is recognized to cause adverse metabolic side effects including obesity, hyperphagia, and hyperglycemia. The Gc receptor (GR) is widely expressed in the brain including the hypothalamus which is known to regulate energy balance. We have previously established through the administration of corticosterone (Cort) in the drinking w...

ea0059p172 | Obesity & metabolism | SFEBES2018

Reduced PTEN levels enhance the proliferation as well as differentiation of preadipocytes

Kirstein Anna , Penke Melanie , Kiess Wieland , Garten Antje

Background/aim: Patients with germline mutations in the tumor suppressor PTEN frequently develop single or multiple lipomas. PTEN antagonizes the phosphatidylinositide3-kinase/AKT/mechanistic target of rapamycin (PI3K/AKT/mTOR) pathway, which promotes cell proliferation and is involved in adipocyte differentiation. The aim of this study was to investigate the mechanisms leading to aberrant adipose tissue growth using PTEN knock-down cell models....

ea0059p173 | Obesity & metabolism | SFEBES2018

Fructose is metabolised by human subcutaneous adipocytes and can be used as a substrate for de novo lipogenesis

Pinnick Katherine , Cornfield Thomas , Green Charlotte , Hodson Leanne

Introduction: Excessive consumption of free sugars (glucose and fructose) is linked to an increased risk of developing chronic metabolic diseases. Current knowledge of fructose metabolism has focussed on the liver where it is implicated in impaired insulin sensitivity, increased fat accumulation and dyslipidaemia. The long-term effects of elevated fructose consumption on human health are poorly defined and fructose metabolism in subcutaneous adipose tissue, the largest human f...

ea0059p174 | Obesity & metabolism | SFEBES2018

Placental DNA methylation is associated with infant adiposity but is not altered with metformin exposure

Yang Liu , Aldhous Marian , Chiswick Carolyn , Norman Jane , Denison Fiona , Drake Amanda , Reynolds Rebecca

Background: Metformin is widely used for treatment of gestational diabetes mellitus. Metformin is considered safe in pregnancy but crosses the placenta. The limited available data of follow-up of children exposed to metformin in utero suggests potential for increased adiposity but mechanisms are unknown. As placental DNA methylation has been linked to later obesity and metformin causes global DNA methylation changes in cancer cell lines we hypothesised that this may be a candi...

ea0059p175 | Obesity & metabolism | SFEBES2018

Hepatic Cyp17a1 regulates the adaptive starvation response aia a nuclear receptor network

Milona Alexandra , Willemsen Ellen , Artigas Natalia , Paterson Helen , Vos Harmjan , Naik Jyoti , Miguel-Aliaga Irene , Bosma Piter , Burgering Boudewijn , Williamson Catherine , Vernia Santiago , Mil Saski van , Owen Bryn

Coupling metabolic processes to nutrient availability is essential for survival. The nuclear receptors PPARα and FXR regulate adaptive liver metabolism in the fasted-state and fed-state, respectively, through a complex mechanism that is incompletely understood. Here, we show that hepatic expression of the steroidogenic enzyme Cyp17al is strikingly regulated by feed-fast cycles via a repressive nuclear receptor cascade involving bile-acid:FXR signalling. Using both gain- a...

ea0059p176 | Obesity & metabolism | SFEBES2018

A Direct Comparison of Metabolic Responses to NAD repletion in C57BL/6J and C57BL/6N diet-induced obesity mouse models

Garten Antje , Cartwright David , Oakey Lucy , Fletcher Rachel , Nasteska Daniela , Hodson David , Larner Dean , Doig Craig , Ludwig Christian , Kluckova Katarina , Lavery Gareth

Background and Aim: Supplementation with precursors of nicotinamide adenine dinucleotide (NAD), was shown to be beneficial in preventing metabolic dysfunction in mice, which is induced by feeding a high fat diet. We compared the effect of nicotinamide riboside (NR) supplementationon whole-body energy metabolism and mitochondrial function in two widely used diet-induced obesity mouse models.Methods: Mice were fed a high fat diet (HFD, 60% fat) or standard...