Introduction: The formation of oxidative stress in the biological reaction of inflammation is accompanied by the formation of methylglyoxal (MG), which initiates the glycosylation reaction of proteins. When hyperglycemia MG content significantly increased. MG interacts with the amino groups of lysine and arginine to form further glycation end products (CNG). Glycation is due to the ability of glucose to form irreversible chemical compounds of CNG with amino groups of proteins. During inflammation, MG covalently binds to proteins, enzymes, lipids, and DNA, in violation of their physiological functions. CNG causes complications of diabetes, damaged proteins in the glomeruli of the kidneys, retina and peripheral nerves. The formation of covalent bonds with the amino groups of collagen is the cause of the loss of vascular elasticity.
Purpose of the study: To determine the level of MG in serum by the method of high performance liquid chromatography according to Titov V.N. 2014 in our modification in patients with NAFLD and type 2 diabetes.
Materials and methods: Surveyed 98 patients with NAFLD and 52 patients with type 2 diabetes and 22 healthy volunteers. Age 57.3±5.2 years. IMT 34.85±1.79. The diagnosis was established on the basis of clinical laboratory data and examination results. The level of POL was determined by the content of malonic dialdehyde (MDA).
The results: In patients with NAFLD, the MG content in the serum was 520.75±114.35 in the control 69.02±6.67 nM/l P=0.001. For T2DM, the content of MG292 is 11±16.34 nM/l. With hyperglycemia and excessive accumulation of lipids, the processes of lipid peroxidation enhance, which leads to an increase in the activity of PLA2. In apoptosis, the function of cellular membrane phospholipids is impaired, the function of mitochondria is disturbed, fibrosis develops and steatohepatitis is formed. The correlation of MDA and PLA2 in the serum r=−0.578 P=0.001 was noted. MG and MDA are in the correlation dependence r=0.495. The content of PLA2 correlates with the level of nitric oxide r=0.625 P=0.001.
Conclusion: In patients with NAFLD there is a significant increase in MG in serum by 7 times compared with the control. MG damages arginine residues of proteins, the insulin signaling is disturbed, and enzymes are inhibited. MG plays a key role in the development of IR and hyperglycemia. Quantitative determination of MG in serum by HPLC is a prognostic and diagnostic test.
18 - 21 May 2019
European Society of Endocrinology