ISSN 1470-3947 (print) | ISSN 1479-6848 (online)

Endocrine Abstracts (2019) 63 P248 | DOI: 10.1530/endoabs.63.P248

Study of relationship between levels of kisspeptin and neurokinin B and markers of calcium-phosphate metabolism and water-electrolyte balance in patients with neuroendocrine disorders

Ekaterina Pigarova, Svetlana Vorotnikova, Artem Zhukov, Larisa Dzeranova, Alexandra Petrushkina & Larisa Nikankina

Endocrinology Research Centre, Moscow, Russian Federation.

Objective: To study the effect of neurohormones kisspeptin and neurokinin B on the indices of calcium-phosphate and electrolyte metabolism in patients with neuroendocrine pathology.

Material and Methods: Eighty-two female patients were included in this pilot study divided into 2 groups: ‘Cushing’s disease’ group (n=51) and ‘Acromegaly’ group (n=31). Median age was 33 years [27; 38] in ‘Cushing’s disease’ group and 39 years [32; 45] in ‘Acromegaly’ group. Kisspeptin levels were measured using an ELISA test-system ‘Kisspeptin-54 S-1308’ (Peninsula Laboratories International, Inc., USA) on a Luminometer Photometer LMA01 (Beckman Coulter, Czech Republic). Extraction of peptide from plasma was performed according to the manufacturer’s protocol. Measurement of the level of neurokinin B was carried out by the ELISA method on Cobas 6000 Module e601 analyzer (Roche, Switzerland), set ‘Neurokinin B S-1271’ (Peninsula Laboratories International, Inc., USA).

Results: The median serum levels of kisspeptin in the ‘Cushing’s disease’ and ‘Acromegaly’ groups were 10.8 [3.0; 13.5] and 7.9 [0.14; 11.7] ng/ml, neurokinin B – 0.11 [0.08; 0.13] and 0.07 [0.06; 0.1] ng/ml, sodium - 142 [140; 143] and 140 [139; 141] mmol/l, potassium – 4.4 [4.2; 4.8] and 4.6 [4.2; 5.0] mmol/l, chlorides - 106 [105; 108] and 107 [105; 109] mmol/l, total calcium – 2.39 [2.28; 2.46] and 2.38 [2.31; 2.45] mmol/l, phosphate – 1.14 [1.07; 1.26] and 1.34 [1.24; 1.53] mmol/l respectively. We observed statistically significant difference in kisspeptin, sodium and phosphate levels between groups (P<0.05). In the correlation analysis, no significant dependencies were obtained between neuropeptides and sodium, potassium and chloride blood levels. Negative correlation was observed between kisspeptin and neurokinin B in ‘Cushing’s disease’ group (r=−0.55, P<0.05), between kisspeptin and serum phosphate in ‘Acromegaly’ group (r=−0.45, P<0.05).

Conclusion: In a pilot study assessing the impact of new neuroendocrine hormones, there is no correlation between kisspeptin, neurokinin B and sodium, potassium, chloride levels, which negates their significant role in the control of water and electrolyte parameters of blood. Relationship between the studied neuroendocrine hormones and calcium-phosphate metabolism is not clearly understood and may be the subject of further research.

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