Objective: To study the effect of neurohormones kisspeptin and neurokinin B on the indices of calcium-phosphate and electrolyte metabolism in patients with neuroendocrine pathology.
Material and Methods: Eighty-two female patients were included in this pilot study divided into 2 groups: Cushings disease group (n=51) and Acromegaly group (n=31). Median age was 33 years [27; 38] in Cushings disease group and 39 years [32; 45] in Acromegaly group. Kisspeptin levels were measured using an ELISA test-system Kisspeptin-54 S-1308 (Peninsula Laboratories International, Inc., USA) on a Luminometer Photometer LMA01 (Beckman Coulter, Czech Republic). Extraction of peptide from plasma was performed according to the manufacturers protocol. Measurement of the level of neurokinin B was carried out by the ELISA method on Cobas 6000 Module e601 analyzer (Roche, Switzerland), set Neurokinin B S-1271 (Peninsula Laboratories International, Inc., USA).
Results: The median serum levels of kisspeptin in the Cushings disease and Acromegaly groups were 10.8 [3.0; 13.5] and 7.9 [0.14; 11.7] ng/ml, neurokinin B 0.11 [0.08; 0.13] and 0.07 [0.06; 0.1] ng/ml, sodium - 142 [140; 143] and 140 [139; 141] mmol/l, potassium 4.4 [4.2; 4.8] and 4.6 [4.2; 5.0] mmol/l, chlorides - 106 [105; 108] and 107 [105; 109] mmol/l, total calcium 2.39 [2.28; 2.46] and 2.38 [2.31; 2.45] mmol/l, phosphate 1.14 [1.07; 1.26] and 1.34 [1.24; 1.53] mmol/l respectively. We observed statistically significant difference in kisspeptin, sodium and phosphate levels between groups (P<0.05). In the correlation analysis, no significant dependencies were obtained between neuropeptides and sodium, potassium and chloride blood levels. Negative correlation was observed between kisspeptin and neurokinin B in Cushings disease group (r=−0.55, P<0.05), between kisspeptin and serum phosphate in Acromegaly group (r=−0.45, P<0.05).
Conclusion: In a pilot study assessing the impact of new neuroendocrine hormones, there is no correlation between kisspeptin, neurokinin B and sodium, potassium, chloride levels, which negates their significant role in the control of water and electrolyte parameters of blood. Relationship between the studied neuroendocrine hormones and calcium-phosphate metabolism is not clearly understood and may be the subject of further research.
18 - 21 May 2019
European Society of Endocrinology