ISSN 1470-3947 (print) | ISSN 1479-6848 (online)

Endocrine Abstracts (2019) 63 P564 | DOI: 10.1530/endoabs.63.P564

Erythrocytes glucose uptake increases in rats with experimental induced obesity, diabetes and hyperinsulinemia

Zulaykho Shamansurova1, Anna Alieva1,2, Akmaral Tashmanova3 & Talat Saatov1,4

1TashPMI, Tashkent, Uzbekistan; 2RSSPMCE, Tashkent, Uzbekistan; 3Cardilogy and Internal Diseases, Almaty, Kazakhstan; 4Bioorganich Chemistry, Tashkent, Uzbekistan.

Introduction: As were previously shown the erythrocytes glucose uptake is impaired in patients with the Diabetes Mellitus, obesity and polycystic ovarian syndrome due to insulin resistance. For further, study whether the erythrocytes glucose uptake will be impaired in rats with experimental metabolic syndrome were observed gradually.

Material and methods: The 40 adult white male rats separated into 4 groups. In 10 rats diabetes were induced by injection of 200 mkg/kg alloxan (AID), in other 10 rats obesity were induced by diet (DIO), in other 10 rats hyperinsulinemia induced by daily sc injection of insulin 0.1 mU/kg during one month (HIR) and another 10 rats were stay intact as a control group (N). Rats body weight (BW), glycemia, HbA1c level and erythrocytes glucose uptake (EGU) observed weekly during 2 months and compare to initial data in the beginning (ID) in each group.

Results: Final BW were significantly increased in DIO group by 20.14%, P<0.01 and in HIR by 17.62%, P<0.05 than ID. The fasting glycemia level were increased only in AID by 2 times (P<0.05), whereas HbA1c level were increased by 62% (P<0.01) in AID, by 44% (P<0.01) in HIR and 26% (P<0.05) in DIO. Interestingly, EGU level were increased by 109% (P<0.01) in AID, by 130% (P<0.01) in HIR, by 58% (P<0.05) in DIO group than ID and show the strong linkage with HbA1c level in all groups. Analysis in dynamics shown gradually increasing of the EGU in AID in the first week, in DIO in the 2nd week and in HIR in the 3rd week of experiment.

Conclusion: EGU were increased in rats with experimental models of metabolic syndrome AID, HIR, DIO, dependent from HbA1c level and can be used as a marker in early diagnosis.

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