Introduction: Modifications of gut microbiota have been described in relation to inflammatory bowel disease (IBD), dietary habits and nutritional status.
Objective: To relate gut microbial pattern of patients with IBD compared to healthy individuals; and link gut microbial pattern with activity of the disease, nutritional status and dietary habit.
Material and methods: Observational study of 56 subjects (44 with IBD and 12 controls). We collected clinical and analytical data (nutritional and inflammatory profile), anthropometric, dietary habits (record of 3 days) and microbial study (grouped by a dendrogram for Lactobacillus and the clusters were amplified by PCR).
Results: Mean age 44.7±2.07 years (54.5% males). The disease ratio Crohns disease (CD)/ulcerative colitis (UC) was 23/21. 38.6% presented remission of the disease and the rest had mild (27.3%), moderate (27.3%), severe activity (11.4%). 37% with activity had malnutrition and 76.9% had moderate or severe vitamin D deficiency (P=0.025, 11.61±5.42 vs 21.99±1.81). In the analysis of gut microbiota we found greater similarity between controls and those patient in remission (M1 pattern). The other microbial pattern (M2) showed lower bacterial diversity (patients with higher use of corticosteroid therapy). However, M1 had a higher vitamin D deficiency (75% vs 52.4%) and malnutrition (37.5% vs 22.7%). M2 was associated with the highest protein and carbohydrate intake: meat (P=0.003, 60.63±32.55 vs. 128.73±10.26); dairy products (P=0.040, 137.13±64.43 vs 285.36±34.28) and pasta (P=0.049, 15.88±8.62 vs 40.82±8.23). However, malnourished patients took more vegetables (P=0.024, 161.25±43.1) and less fat products (P=0.032, 74.84±6.34 vs 92.93±4.24).
Conclusions: Gut microbiota pattern changes according to diet. M2 (more Firmicutes and Proteobacteria and less Bacteroidetes); Active IBD (M2) is associated with a Western diet. The dietary influence on gut microbiota is greater than inflammatory pattern.
18 - 21 May 2019
European Society of Endocrinology