Introduction: Obesity and type 2 diabetes mellitus (T2DM) are commonly associated with coronary artery disease (CAD). Recent studies demonstrated that microRNAs (miRNAs) are involved in the pathogenesis of CAD.
Aim: To study miRNA-26a, microRNA-33a and microRNA-33b expression in patients with obesity.
Methods: MiRNAs were detected in peripheral blood samples of 66 patients, aged 4865 years. Patients with BMI 30.039.9 kg/m2 were divided into 3 groups. The 1st group included 21 patients with CAD (confirmed by coronary angiography) and T2DM, the 2nd group 22 patients with T2DM and excluded CAD (according to the treadmill test), the 3rd group 23 patients with obesity and excluded CAD and T2DM (healthy obesity). RT-qPCR assays were used for miRNA detection.
Results: Expression of miRNA-33a was significantly different in the studied groups (P=0.009). MiRNA-33a was positively correlated with brachiocephalic arteries stenosis (r=0.453; P=0.039). MiRNA-33b was positively correlated with BMI (r=0.293; P=0.008) and HbA1c (r=0.269; P=0.016). MiRNA-33b was positively correlated with the thickness of interventricular septum (r=0.281; P=0.012); negatively with ejection fraction (r=0.397; P=0.0003) and triglycerides (r=0.551; P=0.009). In the third group, miRNA-33a and b were positively correlated with waist circumference (r=0.444; P=0.034 and r=0.438; P=0.036, respectively); negatively with cholesterol (r=0.419; P=0.046 and r=0.489; P=0.018, respectively). MiRNA-33b also negatively correlated with brachiocephalic arteries stenosis (r=0.474; P=0.022). MiRNA-26a expression was lower in patients of the 1st group (with T2DM and CAD (P=0.003). In the first group of patients, miRNA-26a was positively correlated with LDL-cholesterol (r=0.541; P=0.011) and waist circumference (r=0.481; P=0.027). MiRNA-33a and -b were positively correlated with miRNA-26a (r=0.375; P=0.001 and r=0.353; P=0.001, respectively).
Conclusions: Expression of miRNA-33a differ in patients with CAD, T2DM, and healthy obesity. MiRNA-33a and miRNA-33b were positively correlated with heart remodeling processes and therefore may determine the severity of cardiovascular disease in patients with obesity.
18 - 21 May 2019
European Society of Endocrinology