Endocrine Abstracts

Resin-based composite materials have become most used in restorative dentistry as a result of continuous effort to reduce the use of dental amalgam. Since 2018, it has been banned in children under 15 years of age, pregnant and nursing women. As these materials often contain bisphenol A glycidyl methacrylate (Bis-GMA) or derived monomers, bisphenol A (BPA), known for its endocrine disrupting effects, may be released and represent a low-dose and long-term source of BPA for the human body. To evade the problem of BPA presence, its structural derivatives such as BPS, BPF, and BPAF are often used in industry. The aim of this work was to determine the maximal amounts of BPA released from Bis-GMA-containing and ‘BPA-free’ restorative composites, and to describe the kinetics of BPA release to methanol using liquid chromatography tandem mass spectrometry (LC-MS/MS). Bis-GMA-containing composites Charisma Classic (CC) and Filtek Ultimate (FU), and ‘BPA-free’ Charisma Diamond (CD) and Admira Fusion (AF) were used in this study. Specimens (diameter 6 mm, height 2 mm, n = 5) were light-cured from one side (20 s, 1000 mW/cm²), and stored at 37 °C in 2 ml of methanol which was exchanged after 1, 4, 9, 16, 35, 65, and 130 days. Bisphenol concentrations were measured using LC-MS/MS. In order to gain high sensitivity, dansyl chloride derivatization was carried out¹. The total amounts of BPA released from ‘BPA-free’ materials CD (8.31 ± 1.47 ng/g) and AF (5.18 ± 1.30 ng/g) after 130 days were significantly lower compared with Bis-GMA-containing composites CC (146.17 ± 6.99 ng/g) and FU (182.61 ± 5.94 ng/g). The highest release of BPA was observed within the first day of incubation, followed by a gradual decrease. The release was still ongoing since the 35-days values at a similar daily rate until 130 days of incubation. Alternative bisphenols (BPS, BPF, BPAF) were not detected. As the total amounts of BPA released within 130 days were several orders of magnitude lower than the tolerable daily intake 4 µg/kg body weight/day, these materials should not pose a serious health risk according to the current criteria.

Reference

1. Kolatorova SL, Chlupacova T, Vitku J, Vlk M, Heracek J, Starka L, Saman D, Simkova M, Hampl R, Talanta 2017 174 21.