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Endocrine Abstracts (2022) 81 OC13.4 | DOI: 10.1530/endoabs.81.OC13.4

ECE2022 Oral Communications Oral Communications 13: Adrenal and Cardiovascular Endocrinology 2 (6 abstracts)

Classic and 11-oxygenated androgens in serum and saliva across adulthood and the menstrual cycle – a mass spectrometry-based cross-sectional study

Punith Kempegowda 1,2 , Lina Schiffer 1 , Jo Adaway 3 , Fozia Shaheen 1 , Andreas Ebbehoj 4,5 , Sumitabh Singh 4 , Alessandro Prete 1,2 , James M Hawley 1,3 , Alice J Sitch 6 , Brian Keevil 3 , Irina Bancos 4 , Angela Taylor 1 & Wiebke Arlt 1


1University of Birmingham, Institute of Metabolism and Systems Research, United Kingdom; 2Queen Elizabeth Hospital Birmingham, United Kingdom; 3Wythenshawe Hospital, Department of Clinical Biochemistry, United Kingdom; 4Mayo Clinic, Division of Endocrinology, Metabolism, Diabetes and Nutrition, Department of Internal Medicine, United States; 5Aarhus University, Department of Clinical Medicine, Aarhus, Denmark; 6University of Birmingham, Institute of Applied Health Research, Birmingham, United Kingdom

Objective: Quantify classic and 11-oxygenated androgens in serum and saliva and determine variations across age, sex, body mass index (BMI), menstruation and hormonal contraception use.

Methods: Morning serum samples were collected from 292 healthy volunteers (125 men, 22-95 years; 167 women, 21-91 years). Morning saliva was collected from 83 healthy volunteers (51 women, 32 men). 25 individuals (12 women, 13 men) also collected a 7-timepoint diurnal saliva profile; the 12 women also collected morning saliva on seven consecutive days during both follicular and luteal phase. The following steroids were quantified by liquid chromatography-tandem mass spectrometry: classic androgens and their precursors (dehydroepiandrosterone sulfate [DHEAS], dehydroepiandrosterone [DHEA], androstenedione [A4], testosterone [T], dihydrotestosterone [DHT]), and 11-oxygenated androgens and their precursors (11-hydroxy androstenedione [11OHA4], 11-keto androstenedione [11KA4], 11-hydroxy testosterone [11OHT], 11-keto testosterone [11KT]). Data were pooled Descriptive statistics and non-parametric tools were used for each variable to obtain median, IQR and significance (p-values). Multiple linear regressions were performed to delineate the distinct effects of age and BMI in a sex-specific analysis

Results: Age: In serum, DHEAS, DHEA, and A4 decreased with age in both men and women, while 11OHA4, 11KA4, 11OHT, 11KT remained stable. Sex: Serum concentrations of DHEA, A4, and 11-oxygentated androgens were similar in men and women while, as expecteded, T and DHT were higher in men. 11OHA4 levels were the highest of all 11-oxygenated androgens and were higher than A4 and similar to DHEA levels. BMI: There were no associations of DHEAS, DHEA, A4, 11OHA4 and 11KA4 with BMI. After adjusting for age, 11KT positively correlated with BMI in men (change/kg/m2 (95% CI)=3.05(0.08, 6.03), P=0.044), while the relationship between 11OHT and BMI was not significant. Menstruation status: Saliva classic and 11-oxygenated androgens showed a clear diurnal pattern in men and in the follicular phase in women, but in the luteal phase only 11-oxygenated androgens showed diurnal variation. Postmenopausal women had lower serum DHEAS, DHEA, A4, T, and 11KA4 (P<0.001) compared to premenopausal women. 11OHT and 11KT were increased in postmenopausal compared to premenopausal women (P<0.001 and P=0.005, respectively). Impact of contraception: Women on hormonal contraceptives had lower T and 11-KT concentrations in saliva, but not in serum.

Conclusion: While classic androgens decline with age and are subject to menstrual cycle-dependent variation, 11-oxygenated androgens form a stable pool during adulthood. While all other measured androgens decreased after menopause, 11OHT and 11KT increased, which may have clinical implications for diagnostic work-up of hormonal pathologies.

Volume 81

European Congress of Endocrinology 2022

Milan, Italy
21 May 2022 - 24 May 2022

European Society of Endocrinology 

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