Endocrine Abstracts

Objectives: The crystal structures of the TSH receptor (TSHR) leucine rich repeat domain (LRD) bound to TSHR stimulating monoclonal autoantibody M22TM or to TSHR blocking monoclonal autoantibody K1-70TM and antibody free have been solved previously. Cryo-electron microscopy (cryo-EM) was now used to solve the structure of full length TSHR in complex with K1-70TM.Methods: Recombinant human TSHR expressed in CHO cells was i...

Objective: Vitamin D has potential immunomodulatory effects. We studied whether vitamin D3 supplementation affects the course of Graves’ disease (GD).Methods: In a double-blind, placebo-controlled design, we randomized patients with a first time diagnosis of GD hyperthyroidism to daily supplementation with vitamin D3 70 mg (2800 IU) or placebo, as add-on to standard treatment with anti-thyroid drugs (ATD). The intervention was continued 12 months af...

Objective: Thyroid hormones are essential for maintaining metabolic balance and particularly influence lipid synthesis and degradation. Metabolomics and in-depth lipid profiling may enable us to assess for differential effects of thyroid hormones and provide insight into tissue thyroid status, that cannot be captured by levels of serum free thyroid hormones alone.Methods: 4,347 children from the Avon Longitudinal Study of Parents and Children who had thy...

Objectives: Mutations in SECISBP2 cause deficiency of selenoproteins, resulting in a multisystem disorder with abnormal circulating thyroid hormone and selenium levels and features due to lack of specific selenoproteins or loss of antioxidant selenoenzymes. Having observed early-onset, aneurysmal thoracic aortic dilatation in four patients with this disorder, we studied zebrafish and murine Secisbp2 mutant models to determine whether the aortic phenotype and selenopro...

Objective: Recent studies have shown that sarcopenia at cancer diagnosis is associated with a poor survival in patients with solid tumors. Up to now, few data are available among patients with advanced thyroid cancer, especially those treated with tyrosine kinase inhibitors (TKI). We retrospectively evaluated sarcopenia by Skeletal Muscle Index (SMI) in a cohort of advanced thyroid cancer patients before and during TKI treatment and investigated its association with treatment ...

Introduction: Due to their remarkable self-organizing structures and functional properties, organoid has become a powerful tool to model diseases ‘in a dish’. The use of organoids in cancer research emerged to better understand tumor behavior. Originally, adult stem cells (aSC)-derived organoids were derived from primary tumors while recent studies have reported the generation of cancer models arising from healthy cells by controlling oncogene expression. SC-derived ...