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Endocrine Abstracts (2022) 86 P129 | DOI: 10.1530/endoabs.86.P129

SFEBES2022 Poster Presentations Reproductive Endocrinology (36 abstracts)

Adipocytes-Derived Extracellular Vesicle-miR-26b Induces the process of Polycystic Ovary Syndrome

Guannan Zhou 1,2 , Yuanyuan Gu 1 , Fangyue Zhou 1 , Keqin Hua 1 & Jingxin Ding 1


1Obstetrics and Gynecology Hospital of Fudan University, Shanghai, China; 2Karolinska Institutet, Stockholm, Sweden


Background: Adipocyte cells could produce numerous extracellular vesicles and orchestrate the balance of endocrinological system. Polycystic ovary syndrome (PCOS) is a refractory reproductive disease and also a kind of endocrine and metabolic disease. The aim of the study was to explore the effects of adipocyte-derived extracellular vesicles-miR-26b on cumulus cells (CCs) and development of PCOS.

Methods: The crosstalk mediated by extracellular vesicle-miR-26b between adipocytes and CCs was determined in CC cells co-cultured with mature adipocytes or incubated with extracellular vesicle isolated from mature adipocytes. CCK-8 assay and flow cytometry were conducted in CCs treated with or without extracellular vesicles; microRNA (miRNA) sequencing was conducted for clarifying the key molecular. Hormone levels and ovary ovulation ability were conducted with animal experiment.

Results: The results indicated that mature adipocytes derived extracellular vesicles highly expressed miRNA-26b. Adipocyte-derived extracellular vesicles inhibited viability and promoted apoptosis in CCs via targeting JAG1. Furthermore, extracellular vesicles derived from mature adipocyte disrupted the ovary ovulation and impaired the hormone levels.

Conclusions: These results identify a novel signaling pathway that adipocytes-derived extracellular vesicles-miR-26b promotes cell apoptosis in CCs and disrupted the ovary ovulation in the development of PCOS. The study indicates that adipose tissue-derived extracellular vesicles-miR-26b may play a key role in the PCOS and also provides insight into developing new therapeutic strategies for PCOS.

Volume 86

Society for Endocrinology BES 2022

Harrogate, United Kingdom
14 Nov 2022 - 16 Nov 2022

Society for Endocrinology 

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