ECE2023 Poster Presentations Diabetes, Obesity, Metabolism and Nutrition (159 abstracts)
Assessment of insulin-releasing and glucose-lowering actions of aqueous extracts of Pterocarpus mildbraedii cellular and diet-induced mice models of type 2 diabetes
Opeolu Ojo 1,2 , Ayokunle Benjamin Falana 1 , Ayodele Abiodun Falobi 1 , Simren Kaur Heer 1 , Mojisola Adie 1 , Joy Nneka Edeani 1 , Gabriel Anyanwu 3 & Uju Ejike 3
1University of Wolverhampton, Wolverhampton, United Kingdom; 2IRiD Bioscience, Stokes-on-Trent, United Kingdom; 3Bingham University, Department of Biochemistry, Karu, Nigeria
Aim: The use of commonly consumed vegetables, such as Pterocarpus mildbraedii, in the treatment of metabolic conditions is consistent with the food-as-medicine strategy. To characterise its pharmacological actions, this study assessed insulin-releasing and glucose lowering effects of effects of P. mildbraedii in models of type 2 diabetes.
Methods: Clonal pancreatic cells, BRIN-BD11, were treated P. mildbraedii extracts (0 - 0.1-1000µg/ml) in the presence or absence of insulin secretagogues, inhibitors and extracellular calcium. Cytotoxicity and actions of P. mildbraedii on cell viability, cell proliferation, intracellular calcium concentration, membrane depolarisation and glucose uptake were assessed together with its in vivo actions on diet-induced diabetes.
Results: P. mildbraedii stimulated non-toxic concentration-dependent insulin secretion at all concentrations tested (0.1-1000µg/ml, 1.1-3.4-fold, P<0.01-0.001). P. mildbraedii extract produced no significant effect on cell viability. Actions of the extract were inhibited by verapamil (50nM, 49%, P<0.001), diazoxide (300µM, 50%, P<0.01) and absence of calcium (47%, P<0.01). Insulinotropic effects increased in incubations containing KCl (30mM, 1.2-fold, P<0.05) and different glucose concentration (1.1 to 5.6mM, 1.8-fold, P<0.01; 5.6 to 16.7mM, 1.1-fold, P<0.05). Enhanced cell proliferation (21%, P<0.01), membrane depolarisation (27%, P<0.05), intracellular calcium concentration (31%, P<0.001), glucose uptake (31%, P<0.01) were observed in vitro. Glucose tolerance improved (42%, P<0.01) in high-fat fed mice.
Conclusions: These results indicate significant antidiabetic actions of P. mildbraedii and suggests the involvement of the ATP-dependent pathway of insulin secretion.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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