Searchable abstracts of presentations at key conferences in endocrinology

ea0032p489 | Diabetes | ECE2013

Analysis of the newly diagnosed diabetic patients, one year and five years of evolution, C-peptide, autoinmunity, treatment and metabolic control

Dominguez-Lopez Marta , Fernandez-Arias David , Gonzalez-Molero Inmaculada , Colomo Natalia , Adana Marisol Ruiz de , Soriguer Federico

Objective: We retrospectively analyzed patients with type 1 diabetes onset, attended during the years 2005 and 2006 on the specific query of type 1 DM debut of a tertiary hospital, with initial treatment assessment, at 1 and 5 years evolution.Results: Patients were treated for newly diagnosed DM 1, 25 patients (72% male, 28% female) mean age 23.2 years (14–36), mean BMI: 23.18 (18–33.8%).The clinical presentation included: 88.24% presented with...

ea0032p540 | Endocrine tumours and neoplasia | ECE2013

PKCδ plays an important in regulating human medullary thyroid carcinoma cell viability

Mole Daniela , Gentilin Erica , Gagliano Teresa , Tagliati Federico , Ambrosio Maria Rosaria , degli Uberti Ettore , Zatelli Maria Chiara

Protein kinase C (PKC) is a family of serine-threonine kinases that regulate many cellular processes including proliferation and survival. Previous evidence has shown that PKC is involved in the control of human medullary thyroid carcinoma (MTC) proliferation and survival by modulating apoptosis, with a mechanism that implicates PKCβII isoform and translocation in different subcellular compartments.In this study, we investigated the role of PKC&#948...

ea0029p1124 | Neuroendocrinology | ICEECE2012

Autoimmune thyroid disease and neuroendocrine gastric tumors: early detection and efficacy of somatostatin analogue treatment

Accardo G. , Renzullo A. , De Bellis A. , Bizzarro A. , Romano M. , Federico A. , Gravina A. , Faggiano A. , Marotta V. , Esposito D. , Bellastella A. , Pasquali D.

Autoimmune chronic thyroid disease (AITD) is associated with atrophic gastritis (CAG) in 29–42% of the cases. CAG and the consequent hypergastrinemia, listed as precancerous lesion, can predispose to neuroendocrine cells hyperplasia and the development of neuroendocrine gastric tumors (NET). Aim of this study was to evaluate the association of AITD with risk of gastric NET and the efficacy of somatostatin analogues treatment.Patients: From 2009 to 2...

ea0028p241 | Pituitary | SFEBES2012

Can the stability of variant aryl hydrocarbon receptor interacting protein (AIP) be a marker for pathogenicity in FIPA (familial isolated pituitary adenoma)?

Martucci Federico , Trivellin Giampaolo , Garcia Edwin , Dalantaeva Nadezhda , Chapple Paul , Pecori Giraldi Francesca , Grossman Ashley , Korbonits Marta

Background: 20% of the familial isolated pituitary adenoma (FIPA) population harbour an aryl hydrocarbon receptor-interacting protein (AIP) gene mutation. The recognition of whether a variant is pathogenic can be difficult, in cases where the observed change does not lead to a truncated protein. Segregation with disease in a family, in silico predictions, loss of heterozigosity in the tumour, in vitro functional studies and screening of the variant in controls may help in asce...

ea0022oc2.3 | Thyroid | ECE2010

Allelic discrimination in the diagnosis of somatic BRAF V600E mutation on fine-needle aspiration biopsies

Buratto Mattia , Tagliati Federico , Trasforini Giorgio , Leoni Stefania , Rossi Roberta , degli Uberti Ettore C , Zatelli Maria Chiara

Many studies demonstrated that somatic BRAF gene mutation analysis increases diagnostic accuracy for papillary thyroid carcinoma (PTC), even from very small samples. The gold standard for point mutations research is direct sequencing, that implies DNA extraction, PCR with specific primers, sequencing reaction and run on an automatic sequencer. This is an expensive and time consuming method, and the possible contamination with wild-type DNA not coming from the nodule significan...

ea0022p384 | Endocrine tumours &amp; neoplasia (<emphasis role="italic">Generously supported by Novartis</emphasis>) | ECE2010

Real-time PCR is useful to detect menin gene deletions

Zatelli Maria Chiara , Filieri Carlo , Tagliati Federico , Buratto Mattia , Calabro Veronica , Ambrosio Maria Rosaria , degli Uberti Ettore C

Familial pituitary adenoma is frequently associated with germinal mutations of several genes, including menin gene. MEN1 syndrome is an autosomic dominant disease, characterized by parathyroid adenomas, endocrine gastroenteropancreatic tumors, and pituitary adenomas, due to inactivating mutations of the MenI gene (11q13). MEN1 mutations are scattered within and around the menin open reading frame and are mainly represented by single nucleotide polymorphisms (SNPs), and ...

ea0022p385 | Endocrine tumours &amp; neoplasia (<emphasis role="italic">Generously supported by Novartis</emphasis>) | ECE2010

Role of piuitary tumour transforming gene 1 in medullary thyroid carcinoma

Zatelli Maria Chiara , Tagliati Federico , Buratto Mattia , Pelizzo Mariarosa , Pansini Giancarlo , Ambrosio Maria Rosaria , degli Uberti Ettore C

Pituitary tumour transforming gene 1 (PTTG1) is over-expressed in a variety of endocrine-related tumors. We investigated PTTG1 expression in human C-cell hyperplasia (CCH), human medullary thyroid carcinoma (MTC) and in the human MTC cell line, TT. PTTG1 expression was significantly higher (P<0.01) in CCH (threefold), in papillary thyroid cancer and in MTC (fivefold) than in normal thyroid, and in MTC lymph-node metastases as compared to primary lesions (approximate...

ea0022p386 | Endocrine tumours &amp; neoplasia (<emphasis role="italic">Generously supported by Novartis</emphasis>) | ECE2010

Everolimus reduces human ACTH-secreting pituitary adenoma in vitro cell viability

Zatelli Maria Chiara , Minoia Mariella , Filieri Carlo , Tagliati Federico , Mole Daniela , Scanarini Massimo , Ambrosio Maria Rosaria , degli Uberti Ettore

Everolimus (RAD001), an immunosuppressant drug, has antineoplastic activity in human neoplasia, including endocrine tumors, due to its ability to inhibit the AKT down-stream signaling pathway. It has been demonstrated that AKT is overexpressed and up-regulated in pituitary tumor, including ACTH-producing pituitary tumors, that are still orphan of an effective medical therapy. We therefore investigated the effects of RAD001 on cell viability, apoptosis and mTOR phosphorilation ...

ea0022p387 | Endocrine tumours &amp; neoplasia (<emphasis role="italic">Generously supported by Novartis</emphasis>) | ECE2010

GH influences breast cancer chemoresistanec independently of cell cycle perturbations

Minoia Mariella , Filieri Carlo , Tagliati Federico , Mole Daniela , Leoni Stefania , Ambrosio Maria Rosaria , degli Uberti Ettore C , Zatelli Maria Chiara

GH and insulin-like growth factor 1 (IGF1) are known to promote breast carcinogenesis. Even if breast cancer (BC) incidence in not increased in female acromegalic patients, mortality is greater as compared to general population. In order to evaluate whether GH/IGF1 excess might influence BC response to therapy, accounting for the increased mortality, we evaluated the effects of GH and IGF1 on cell proliferation of a BC cell line, the MCF7 cells, in the presence of doxorubicine...

ea0022p659 | Neuroendocrinology and Pituitary (<emphasis role="italic">Generously supported by Novartis</emphasis>) | ECE2010

Study of pituitary function in acromegaloidism

Sierra Wilfredo Guanipa , Catalina Pablo Fernandez , Fernandez Concepcion Paramo , Garcia Elias Alvarez , Olivie Amalia Andrade , Pena Eduardo , Ferrer Federico Mallo

Acromegaloidism includes clinical features of acromegaly with GH and IGF1 normal levels at baseline and dynamic, without findings of extrapituitary or pituitary tumour. It has been associated with various conditions without a definite pattern. The aim was to evaluate whether there are differences in some hormonal pituitary axis from acromegaloid subjects with respect to acromegalic and healthy subjects. We designed a comparative, multicenter, case–control study in three h...