Searchable abstracts of presentations at key conferences in endocrinology

ea0038p207 | Obesity, diabetes, metabolism and cardiovascular | SFEBES2015

Adrenaline mediated metabolic and functional quiescence protects insulin producing cells from hypoxia

Kelly Amy , Smith Rachel , DiBase Samantha , Davenport Hailey , Limesand Sean

Pancreatic islets are susceptible to hypoxia during isolation, culture, and transplantation. An intervention that protects against hypoxia while preserving β-cell function will improve islet quality. Adrenaline suppresses insulin secretion and oxygen consumption in pancreatic islets. These findings suggest that adrenergic action creates metabolic quiescence in β-cells. Our objective was to determine whether adrenaline is protective during hypoxic culture in an insuli...

ea0038p222 | Obesity, diabetes, metabolism and cardiovascular | SFEBES2015

Transcriptional profiles explain insulin hypersecretion following chronic adrenergic stimulation in foetal sheep islets

Kelly Amy , Chen Xiaochuan , Bidwell Christopher , McCarthy Fiona , Limesand Sean

Prolonged increases in foetal plasma noradrenaline occur in pathologies, including intrauterine growth restriction (IUGR). Infusion of noradrenaline into healthy sheep foetuses showed continuous suppression of insulin secretion but produced a compensatory beta-cell response after noradrenaline infusion was terminated, similar to findings with adrenergic antagonists in IUGR foetuses. Hyper-insulin secretion responsiveness was confirmed in isolated foetal islets. To identify mol...

ea0038p140 | Growth and development | SFEBES2015

Hormonal control of β-cell proliferation in fetal ovine pancreatic islets in vitro

Harris Shelley E , Kelly Amy , Davis Melissa A , Anderson Miranda , Forhead Alison J , Limesand Sean W

Hormonal control of pancreatic β-cell development in the fetus during late gestation remains unclear. In fetal sheep hypothyroidism causes an increase in pancreatic β-cell mass in association with increased plasma insulin and leptin concentrations. This study investigated the effects of triiodothyronine (T3), insulin and leptin on proliferation rates in fetal ovine pancreatic β-cells in vitro.All procedures were approve...

ea0038p185 | Obesity, diabetes, metabolism and cardiovascular | SFEBES2015

Transcriptome profiles of human pancreatic islets following transient ischemia identify inflammatory and cell death responses

Kelly Amy , Smith Kate , McCarthy Fiona , Smith Breonna , Badarinarayana Vasudeo , Steyn Leah , Strop Peter , Limesand Sean , Papas Klearchos

Islet transplantation, an effective treatment for Type I Diabetes, is compromised by transient ischemia. Ischemia reduces the viability and function of transplanted islets and may also promote signals that lower islet efficacy. In order to characterize the response of human islets to transient ischemia, high-throughput sequencing was employed to determine global gene expression. Islets isolated from healthy donors (n=4) were either pelleted in microcentrifuge tubes (i...