Searchable abstracts of presentations at key conferences in endocrinology

ea0014p625 | (1) | ECE2007

Effect of sildenafil administration on penile hypoxia induced by cavernous neurotomy in the rat

Vignozzi Linda , Morelli Annamaria , Filippi Sandra , Barbara Vannelli Gabriella , Forti Gianni , Maggi Mario

Objectives: Radical prostatectomy is an effective therapy for men with clinically localized prostate cancer. A significant number of men develop erectile dysfunction after radical prostatectomy (PPED) due to intraoperatory cavernous nerve injury causing hypoxia and fibrosis of corpus cavernosus. We established an experimental model of bilateral cavernous neurotomy (BCN) in the rat in order to investigate whether sildenafil treatment in PPED patients could prevent penile tissue...

ea0032p680 | Neuroendocrinology | ECE2013

High glucose induces hypogonadotropic hypogonadism by interfering with GPR54 signaling in the preoptic area of the hypothalamus

Morelli Annamaria , Vignozzi Linda , Erica Sarchielli , Comeglio Paolo , Filippi Sandra , Rastrelli Giulia , Maneschi Elena , Cellai Ilaria , Barbara Vannelli Gabriella , Maggi Mario

The metabolic syndrome (MetS) is a clustering of metabolic and cardiovascular risk factors, having in insulin resistance the key element. In males, MetS is also associated to hypogonadism. We recently found that high fat diet (HFD)-induced MetS rabbits also developed hypogonadotropic hypogonadism, showing a reduced immunopositivity for gonadotropin relasing hormone (GnRH) in the hypothalamus. In this study, we investigated the pathogenetic link between Mets components and the ...

ea0014oc11.3 | Reproductive endocrinology II | ECE2007

Sex steroid and leptin regulation of KISS1/GPR54 system, a new regulator of the neuroendocrine reproductive axis, in human fetal GnRH-secreting neurons

Morelli Annamaria , Marini Mirca , Mancina Rosa , Vignozzi Linda , Pezzatini Anna , Luconi Michaela , Mungai Sara , Fibbi Benedetta , Filippi Sandra , Barbara Vannelli Gabriella , Forti Gianni , Maggi Mario

The molecular mechanisms underlying the reawakening of hypothalamic GnRH neurons at puberty remain to be elucidated. Recently, the G protein-coupled receptor 54 (GPR54) and its endogenous ligand kisspeptin, encoded by the KISS1 gene, have been involved. In fact, GPR54 mutations cause idiopathic hypogonadotropic hypogonadism in human and mice. We used the previously characterized primary culture of human fetal olfactory GnRH-secreting neurons, FNC-B4, to study in vitro t...

ea0014p624 | (1) | ECE2007

Testosterone regulates RhoA/Rho-kinase signalling in two distinct animal models of chemical diabetes

Vignozzi Linda , Morelli Annamaria , Filippi Sandra , Ambrosini Stefano , Mancina Rosa , Luconi Michaela , Mungai Sara , Barbara Vannelli Gabriella , Zhang Xin-Hua , Forti Gianni , Maggi Mario

The contractile RhoA/Rho-kinase (Rock) signalling pathway is up-regulated in penile tissue in animal models of experimental diabetes and has been proposed to contribute to diabetes-related erectile dysfunction (ED). In previous studies we demonstrated that testosterone (T) restores diabetes-induced ED by influencing the NO/cGMP/PDE5 pathway.AimTo investigate the effect of T on the RhoA/Rock signalling in course of diabetes.MethodsWe used two distinct animal models of chemical ...

ea0014p627 | (1) | ECE2007

Characterization and functional role of an androgen-dependent PDE5 activity in bladder

Filippi Sandra , Morelli Annamaria , Sandner Peter , Fibbi Benedetta , Mancina Rosa , Marini Mirca , Gacci Mauro , Vignozzi Linda , Barbara Vannelli Gabriella , Carini Marco , Forti Gianni , Maggi Mario

Benign prostate hyperplasia (BPH) is the most common disease in the aging male, often comorbid with erectile dysfunction (ED). PDE5 inhibitors (PDE5i, sildenafil, tadalafil and vardenafil) decrease lower urinary tract symptoms (LUTS) in patients with ED and BPH. We studied PDE5 expression and activity in the human bladder and PDE5i effects both in vitro (human and rat) and in vivo (rat). PDE5 is highly expressed in rat and human bladder and immunolocalized in vas...