Searchable abstracts of presentations at key conferences in endocrinology
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9th European Congress of Endocrinology

ea0014p1 | (1) | ECE2007

Human adrenal NCI-H295R cells produce more C19 steroids than NCI-H295A cells – a possible model to study regulation of androgen biosynthesis?

Samandari Elika , Kempna Petra , Hoffer Gaby , Mullia Primus E , Fluck Christa E

The human adrenal cortex consists of three layers in which specific steroid hormones are produced. Human adrenal NCI-H295A (A) and NCI-H295R (R) cells, originate from the same adrenocortical tumor and express all genes essential for steroidogenesis. Therefore they often serve as a suitable model to study human steroidogenesis. No data are available comparing steroidogenesis of A vs. R cells. Assuming no difference, research data from these two cell lines are directly compared....

ea0014p2 | (1) | ECE2007

Effects of ethanol and blokade of synthesis of nitric oxide on level of ACTH in female rats

Djikic Dragoslava , Vranjes-Djuric Sanja , Budec Mirela , Spasic Andjelka

We showed previously that a single dose of ethanol acts as a stressor in female rats (Milovanovic et al., 2003). In order to extend this observation, we investigated whether the effect of ethanol on ACTH is dose-related and possible interactions between nitric oxide (NO) and alcohol on the level of ACTH. To this end, adult female Wistar rats showing diestrus day 1 were treated with: (a) ethanol (2 or 4 g/kg, i.p.), (b) Nω-nitro-L-arginine-methyl ester (L-NAME), whi...

ea0014p3 | (1) | ECE2007

Study of the hypothalamic-pituitary-adrenal axis in patients with the antiphospholipid syndrome

Carignola Renato , Data Valeria , Vitetta Rosetta , Severino Adriana , Bertero Tiziana , Bazzan Mario , Reimondo Giuseppe , Daffara Fulvia , Angeli Alberto , Terzolo Massimo

Objective: The Antiphospholipid Syndrome (APS) is a thrombophilic disorder characterised by recurrent venous and/or arterial thromboses and increased pregnancy morbidity. There is growing evidence supporting a functional interplay between the neuroendocrine and immune system; the hypothalamic-pituitary-adreal (HPA) axis plays a pivotal role in this network. Previous studies have described normal cortisol levels in APS patients while occurrence of acute adrenal failure was repo...

ea0014p4 | (1) | ECE2007

Survey of thyroid function of Hungarian Vizsla population in Hungary

Thuroczy Julianna , Tibold Aniko , Balogh Lajos

The prevalence of hypothyroidism in women of childbearing age is relatively high. The incidence of hypothyroidism during pregnancy has been calculated as between 0.3% and 0.7%. Overt abnormalities in thyroid function are common endocrine disorders affecting more than 19.2% of pregnant women in certain geographic areas of Hungary. 80% of Hungarian inhabitants are living in an iodine deficient area. The aim of this study was to investigate the prevalence of thyroid dysfunction i...

ea0014p5 | (1) | ECE2007

Pancreatic polypeptide (PP) radioimmunoassay in acute phase and regression of cerulein induced pancreatitis

Orlicz-Szczesna Grazyna , Zdanowska-Filipczak Jolanta

Acute pancreatitis is a real medical problem with high patients mortality. Pathogenic interdependence between pancreas follicles function and islet endocrine secretion is under research. PP cells are pancreatic polypeptide (PP) producing cells, they determine about 1% islets, but their function is not completely known yet. Vagus nerve and peptidergic stimulation regulates PP secretion.The aim of study was to estimate cerulein induced pancreatitis effects...

ea0014p6 | (1) | ECE2007

Evaluation of neuroendocrine disfunction in hypothalamo-pituitary-adrenal axis in diagnosis of depressive and non-depressive alcohol-dependent persons

Matovinovic Martina , Katalinic Darko , Maric Andreja , Solter Miljenko , Ivica Josip , Resetic Josip , Murgic Jure , Vrkljan Milan

Acute and chronic alcohol intake and alcohol withdrawal induce dysfunction of neuroendocrine and other regulatory systems. The aims of this study were to assess a possible hypothalamo-pituitary-adrenal (HPA) axis dysfunction in population of alcoholics, using dexamethasone suppression test (DST). The study was approved by local Ethical Committee. The serum and urinary cortisol were compared between the groups of 89 male patients (64.5% depressive and 35.5% nondepressive alcoho...

ea0014p7 | (1) | ECE2007

Effects of melatonin on glutathione peroxidase activity after adriamycin in normal and pinealectomized rats

Dabrowska Katarzyna , Stuss Michal , Gromadzinska Jolanta , Wasowicz Wojciech , Sewerynek Ewa

Adriamycin (ADR) is a potent chemotherapeutic agent, effective in treatment of leukemias, lymphomas and of many solid tumours. However, its clinical usage is often limited by cardiotoxicity, induced by oxygen radical damage of membrane lipids.Melatonin (MEL), is a well-known antioxidant. It has been shown that melatonin can scavenge free radicals, both directly or indirectly, stimulating the activity of antioxidative enzyme, e.g., glutathione peroxidase ...

ea0014p8 | (1) | ECE2007

Is there any role for anti-inflammatory cytokine Interleukin-10 in advanced congestive heart failure?

Farrag Azza , El Gahndour Nouran , Halim Mohab

Background: CHF manifestations can be explained by the biologic effects of tumor necrosis factor-alpha (TNF-alpha). Interleukin-10 (IL-10) has potent deactivating properties and is considered a potent anti-inflammatory cytokine that inhibits TNF-alpha production. This study was designed to examine the role of IL-10 in patients with CHF and to test its correlation with pro-inflammatory markers.Methodology: Fifty patients with CHF were studied. Patients we...

ea0014p9 | (1) | ECE2007

Regulation of GAGEC1, a cancer-testis associated antigen family member, by sex steroid hormones and TGF-beta: implications for prostatic disease

Sampson Natalie , Schuhfried Gerlinde , Plas Eugen , Berger Peter

Prostate homeostasis and function are regulated by complex interactions between the fibromuscular stroma and secretory epithelium via locally-derived and systemic paracrine- and autocrine-acting growth factors and sex steroid hormones. Stromal tissue remodelling due to alterations in transforming growth factor beta (TGF-β) and sex steroid hormone signalling are associated with benign prostatic hyperplasia (BPH) and prostate cancer (PCa), two of the most common proliferati...

ea0014p10 | (1) | ECE2007

Normalization of serum testosterone level alters local GnRH-II and IL-2R mRNA expression in peripheral lymphocytes in patients with idiopathic hypogonadotrophic hypogonadism (IHH)

Tanriverdi Fatih , Akalin Hilal , Caglayan Okay , Demirkoparan Umit , Ozkul Yusuf , Dundar Munis , Bayram Fahri , Kelestimur Fahrettin

Although the existence of the interaction between sex steroids and immune system is well known, the mechanisms of this interaction are still unclear. Recently a second form of GnRH (GnRH-II) has been described in human, which is significantly expressed in immune tissues suggesting a potential function. In a recent in-vitro study it has been demonstrated that GnRH-II decreases local expression of IL-2R in peripheral lymphocytes (1). However in-vivo interactions of...

ea0014p11 | (1) | ECE2007

Blocking undesired leptin action in vivo with leptin antagonists prepared by site-directed mutagenesis of human, ovine, rat and mouse leptin site III

Niv-Spector Leonora , Gonen - Berger Dana , Salomon Gili , Callebaut Isabelle , Djiane Jean , Gertler Arieh

Recent reports have revealed that leptin’s effects are not restricted to central control of body weight, demonstrating instead that leptin is a pleiotropic hormone with a wide variety of different biological actions. Leptin exhibits undesirable effects in autoimmune diseases, in atherosclerosis, and possibly in several types of cancer, and increases the risk of cardiovascular disease in obese people. Therefore, preparation of reagents capable of abolishing leptin’s a...

ea0014p12 | (1) | ECE2007

Role of soluble Fas-antigen (sFas), interleukin-6 (IL-6) and vascular endothelial growth factor (VEGF) in adrenocortical carcinoma patients

Kushlinsky Nikolai , Britvin Timur , Kazantseva Irina , Kalinin Arian

The numerous growth factors and cytokines take part in mechanisms of tumor growth and metastasing.The aim of this study was determination of sFas, IL-6 and VEGF serum levels in 19 patients with adrenal cortical carcinoma (11 women and 8 men aged 21–72 years). The control group comprised 40 practically healthy donors (22 women and 18 men aged 19–70 years). sFas, IL-6 and VEGF before adrenalectomy and in the control were measured by ELISA. Mean I...

ea0014p13 | (1) | ECE2007

Rosiglitazone interferes with the inflammatory response induced in human endothelial cells by TNFalpha and IFNgamma through a new mechanism involving extracellular signal-regulated kinases (ERKs)

Lombardi Adriana , Piscitelli Elisabetta , Francalanci Michela , Mello Tommaso , Santarlasci Veronica , Gelmini Stefania , Cantini Giulia , Galli Andrea , Cosmi Lorenzo , Annunziato Francesco , Forti Gianni , Serio Mario , Luconi Michaela

Microvascular endothelium is one of the main character and target involved in the inflammatory response. Upon specific activation, endothelial cells massively recruit Th1 IFNgamma secreting lymphocytes at the inflammatory site. In the present study, we investigate the intracellular signalling mediating TNFalpha (T) and IFNgamma (I) inflammatory response in a human endothelial cell line (HMEC-1) in vitro and the interfering effects of rosiglitazone (RGZ), a peroxisome pr...

ea0014p14 | (1) | ECE2007

Role of growth hormone/insulin like growth factor 1 system in the remodelling process of the right ventricle in top levels rowers

Vitale Giovanni , Galderisi Maurizio , Colao Annamaria , Innelli Pasquale , Guerra Germano , Guerra Ermelinda , Lloyd Dini Frank , Soscia Antonio , de Divitiis Oreste , Lombardi Gaetano

The intensive physical activity is often associated with cardiac changes, particularly involving the right ventricular (RV) chamber. However, the molecular mechanisms involved in the RV physiologic adaptation to long-term training are not completely understood. In the present study we investigated the role of the growth hormone/insulin like growth factor 1 (GH/IGF-1) axis in the RV remodeling of athletes.Nineteen male top levels rowers and 19 age-matched...

ea0014p15 | (1) | ECE2007

Clinical significance of simultaneously determined serum interleukin-6, dehydroepiandrosterone and its sulphate levels in melanoma

Boldizsár Mariann , Vincze Borbála , Gilde Katalin , Bánfalvi Teodora , Fejos Zsuzsanna , Borbola Kinga , Kapuvári Bence , Liszkay Gabriella , Otto Szabolcs

Among well-known circulating melanoma markers 5-S-cysteinyldopa (5-SCD), a precursor of pheomelanin biosynthesis and S-100 beta (S-100B) are extensively investigated. Our earlier observations confirmed that serum concentration of 5-SCD and S-100B correlates well with the stages and progression of the disease. Interleukin-6 (IL-6) is a multifunctional inflammatory cytokine implicated in advanced stage of various diseases and tumour recurrence. Malignant melanoma cells are known...

ea0014p16 | (1) | ECE2007

Changes in growth hormone messenger RNA (GHmRNA) expression in rats anterior pituitary after single Interferon (IFN)alpha administration.

Romanowski Wojciech , Braczkowski Ryszard , Danikiewicz Aleksander , Nowakowska-Zajdel Ewa , Muc-Wierzgon Malgorzata , Muryn Zuzanna , Zubelewicz-Szkodzinska Barbara

Introduction: Interferon alpha (IFN alpha) is a cytokine with pleiotropic effects and via different pathways influences secretion of certain cytokines and hormones. Growth Hormone (GH) secreted from the pituitary has its physiological effects on various target tissues. The question is how IFN alpha administered in various type of diseases influences GH secretion. This study investigated acute effect of IFN-alpha on GH mRNA expression in rat anterior pituitary<p class="abst...

ea0014p17 | (1) | ECE2007

Does stress test influence Interleukin (IL)-2 and IL-8 concentration in serum patients with stable ischaemic heart disease?

Romanowski Wojciech , Szkodzinski Janusz , Kaszuba Andrzej , Blazelonis Adam , Muryn Zuzanna , Anna Pietka-Rzycka , Zubelewicz-Szkodzinska Barbara

Background: There is growing evidence that adhesion molecules, proinflammatory cells and cytokines play an important role in a variety of cardiac pathophysiological conditions; Cytokines are responsible for the modulation of immune and inflammatory processes. It has been suggested that cytokines such as IL-1, IL-2, IL-6, IL-10 and TNF alpha are important modulators of atherosclerotic effects with IL-2 and Interferon γ having a proinflammatory atherogenic effect and IL-8 a...

ea0014p18 | (1) | ECE2007

Concentration of inflammatory factors such as CRP (acute phase response protein) and Interleukin (IL)-6 in serum patients with stable ischemic heart disease during trimetazidine treatment

Szkodzinski Janusz , Szewczyk Marek , Romanowski Wojciech , Danikiewicz Aleksander , Muc-Wierzgon Malgorzata , Zubelewicz-Szkodzinska Barbara

Background: The patomechanism of developing ischemic heart disease (i.h.d.) is stenosis of coronary blood vessels with plaque placed on vascular endothelium built with monocytes/macrophages, foam cells, oxidized LDL, leukocytes, platelets and collagen. Atherosclerotic lesions are heavily infiltrated by cellular components associated with inflammation and influenced by other inflammatory factors. Trimetazidine, a clinically effective antianginal agent acts by optimizing cardiac...

ea0014p19 | (1) | ECE2007

Insulin decreases IGF-I bioactivity in patients with impaired glucose tolerance and in healthy subjects

Arafat Ayman M , Frystyk Jan , Weickert Martin O , Spranger Joachim , Schöfl Christof , Möhlig Mathias

Objectives: Insulin resistance (IR) is a very common metabolic abnormality in obesity, which is often associated with reduced growth hormone (GH) secretion. GH deficiency is associated with increased in intra-abdominal fat and several parameters of the metabolic syndrome. IGF-I improves IR but the IGF-binding proteins are supposed to regulate its bioactivity although only little information exists. We postulated that the elevated insulin levels due to IR do not only suppress G...

ea0014p20 | (1) | ECE2007

Plasma free fatty acids and adipocytokines concentration in relation to insulin sensitivity in patients with anorexia nervosa.

Karczewska-Kupczewska Monika , Kowalska Irina , Straczkowski Marek , Adamska Agnieszka , Nikolajuk Agnieszka , Karolczuk-Zarachowicz Malgorzata , Otziomek Elzbieta , Gorska Maria

Anorexia nervosa (AN) is an eating disorder, resulting in sustained low weight. In AN, similarly to syndromes of lipodystrophy, one observes the significant loss of the adipose tissue. In lipodystrophies, despite the lack of subcutaneous adipose tissue, insulin resistance is observed. Adipose tissue is known as a source of a variety of bioactive peptides, known as adipocytokines. The aim of the present study was to examine the plasma concentration of adipocytokines in relation...

ea0014p21 | (1) | ECE2007

Improved glucose metabolism and altered pancreatic structure in transgenic mice overexpressing betacellulin

Dahlhoff Maik , Herbach Nadja , Wanke Rüdiger , Wolf Eckhard , Schneider Marlon R.

Betacellulin, one of several peptides activating the EGFR (ErbB1) and related receptors, is a multipotent growth factor known to posses the unique ability to promote growth and differentiation of pancreatic β-cells.We investigated the effects of betacellulin overexpression in a recently established transgenic mouse model (Schneider et al., Endocrinology 146, 5237–5246, 2005). In transgenic animals, overall glucose metabolism was improved...

ea0014p22 | (1) | ECE2007

Screening of 120 adipokines in subcutaneous adipose tissue of patients with growth hormone deficiency reveals changed protein levels

Gasperikova Daniela , Ukropec Jozef , Penesova Adela , Sell Henrike , Skopkova Martina , Vlcek Miroslav , Radikova Zofia , Koska Juraj , Belan Vitazoslav , Pura Mikulas , Vanuga Peter , Payer Juraj , Eckel Jurgen , Klimes Iwar

The role of adipokines and inflammatory cytokines of adipose tissue for development of the growth hormone deficiency (GHD)-related metabolic derangements has not yet been completely understood. Therefore, we screened the protein level of 120 adipokines in subcutaneous adipose tissue (ScAT) of patients with GHD in adulthood.Subjects and methods: Sixteen GHD (10M/6F) with BMI 27±1.0 kg/m2, age 30±2 yrs and sixteen controls matched for ...

ea0014p23 | (1) | ECE2007

Human somatotrophic (GH) adenoma cells – interleukin (IL)-1β induces production of il-6 and il-8.

Diness Signe , Rasmussen Æse Krogh , Bendtzen Klaus , Kosteljanetz Michael , Feldt-Rasmussen Ulla

Aim: to establish a human in vitro system for the study of pituitary cells in culture and subsequently to study the influence of the pro-inflammatory cytokines interleukin (IL)-1β and tumour necrosis factor (TNF)-α on the function of the somatotrophic cells inclusive the ability of the cells to produce IL-6 and IL-8.Methods: Pituitary adenomas were obtained from hypophysectomies of patients with acromegaly. The tissue was enzymatically d...

ea0014p24 | (1) | ECE2007

Serum ferritin concentrations in an impaired fasting glucose population and their normal control group

Sharifi Faranak , Jaberi Yahya , Mousavinasab Nuraddin

Background: Some recent studies have revealed the relationship among excess ferritin, coronary heart disease, and insulin resistance. To assess the association between serum ferritin concentration and Impaired Fasting Glucose, a prediabetes situation with insulin resistance, this study was designed in Zanjan, Iran.Materials & Methods: 187 people including 91 impaired fasting glucose (IFG) subjects and 96 normal glucose subjects who had been recognize...

ea0014p25 | (1) | ECE2007

Implications of serum resistin in overweight diabetic patients with ischemic heart disease

Farrag Azza , Salama Sameh , Rizk Amal

Background: Resistin is a recently discovered adipocyte-secreted hormone that links obesity with insulin resistance and/or metabolic and cardiovascular risk. This study was designed to investigate whether serum resistin concentrations constitute a significant coronary risk factor, with a particular focus on diabetes and one of its microvascular complications; nephropathy.Methodology: Serum resistin was measured in 86 overweight patients with acute corona...

ea0014p26 | (1) | ECE2007

The protective effect of tribulus terrestris in diabetes

Amin Amr , Lotfy Mohamed , Adeghate Ernest

Tribulus terrestris (TT) is used in the Arabic folk medicine to Q1 treat various diseases. The aim of this study was to investigate the protective effects of TT in diabetes mellitus (DM). Diabetes is known to increase reactive oxygen species (ROS) level that subsequently contributes to the pathogenesis of diabetes. Rats were divided into six groups and treated with either saline, glibenclamide (Glib), or TT for 30 days. Rats in group 1 were given sa...

ea0014p27 | (1) | ECE2007

Prevalence of I27L polymorphism of hepatic nuclear factor-1[alpha] in diabetic patients younger than 35 years old.

Amador Norma , López Manuel , Sánchez Jose , Guízar Juan , Zapién Alejandra , Martínez Silvia

Objective: In last decades prevalence of type 2 diabetes mellitus (DM) in children and young people worldwide has been reported increase. It is necessary to know according to biochemical and genetic characteristics the frequency of DM no corresponding to type 1 in our population. The objective of this study was to determine the prevalence of mutations on hepatic nuclear factor 1 [alpha], and 4 [alpha] in diabetic patients younger than 35 years old with features of clinical aut...

ea0014p28 | (1) | ECE2007

Novel mechanism of chronic exposure of oleic acid-induced insulin release impairment in rat pancreatic β-cells

Kudo Takanori , Ogawa Yoshiji , Suga Sechiko , Tomotsune Ken , Hasegawa Noriyuki , Suda Toshihiro , Wakui Makoto

A sustained, high circulating level of free fatty acids (FFAs) is an important risk factor for the development of insulin resistance, islet beta-cell dysfunction, and pathogenesis of type 2 diabetes. Here, we report a novel mechanism of chronic exposure of oleic acid (OA)-induced rat insulin release impairment. Following a 4-day exposure to 0.1 mM OA, there was no significant difference in basal insulin release when comparing OA-treated and untreated islets in the presence of ...

ea0014p29 | (1) | ECE2007

Quality of care in a diabetic outpatient clinic

Nobre Ema Lacerda , Chambel Paula , Domingues Andreia , da Silva Marlene Paes , Jorge Zulmira , Santos Valentim , Raimundo Joaquim , de Castro João Jácome

Background and aims: Type 2 Diabetes Mellitus affects a growing number of people allover the world. It is associated with serious complications. Several studies have shown that it is possible to prevent and minimize type 2 diabetes complications if it is treated appropriately over time. In our Hospital there is, since 1998, an outpatient clinics of diabetes. This study aimed to determine the quality of care provided to diabetic patients in our institution.<p class="abstext...

ea0014p30 | (1) | ECE2007

Cardiovascular risk factors (CVRF) as predictors of microalbuminuria (MA) in type 2 diabetes mellitus (T2DM) patients

del Cañizo-Gomez Francisco Javier , Moreira-Andres Maria Natividad

MA is a marker of greatly increased cardiovascular morbidity and mortality in T2DM patients.Objective: To perform a prospective study of normoalbuminuric T2DM patients, analysing the association between CVRF at baseline and the development of MA at follow-up.Materials and methods: The prospective observational study was performed at Montes de Barbanza public health center, a specialized secondary referral center, which provides ser...

ea0014p31 | (1) | ECE2007

The prevalence of metabolic syndrome and its relation to metabolic control in patients with diagnosed type 2 diabetes

Toti Florian , Bejtja Gazmend , Hoti Kliti , Shota Eduart , Thengjilli Erion

Background and Aims: The identification of metabolic syndrome (MS) is important so that components of this syndrome can be managed appropriately to prevent or delay progression of associated cardiovascular risk factors. The aim of our study was to determine the prevalence of the Metabolic Syndrome as the NCEP/ATP III criteria in a selected population of type 2 diabetes from the Tirana Register of Diabetes.Materials and Methods: In Tirana district we rand...

ea0014p32 | (1) | ECE2007

Effects of rosiglitazone (RGZ) and pioglitazone (PGZ) on serum androgens and urinary steroid profile in patients with type 2 diabetes: A prospective, randomised cross-over study

Chappuis Bernard , Braun Monika , Stettler Christoph , Diem Peter , Christ Emanuel

Background: Glitazones (GZ) influence androgen biosynthesis in PCO syndrome. At present it is unknown whether a) steroid hormone metabolism is influenced by GZ in patients with type 2 diabetes b) there is a differential effect of RGZ and PGZ on steroid hormone metabolism c) this effect is sex-specific and d) this effect is mediated by changes in insulin sensitivity. Therefore, urinary steroid profiles and serum total testosterone and DHEA levels were analysed before and after ...

ea0014p33 | (1) | ECE2007

Abnormal glucose challenge test reflects mild gestational diabetes

Kafkasli Ayse , Cikim Ayse Sertkaya , Selcuk Engin Burak , Dogan Kezban , Burak Feza , Yologlu Saim

Objective: The status of carbohydrate metabolism of pregnant women with positive glucose challenge test (GCT), but normal oral glucose tolerance test (OGTT); and their neonates have not defined clearly.Methods: Pregnant women with normal GCT (n: 120), with abnormal glucose challenge test (AGCT) but normal OGTT (n: 67) and those with gestational diabetes (GDM) (n: 67) were included into the study. Local ethical committee approval was ...

ea0014p34 | (1) | ECE2007

Ambulatory blood pressure reduction after rosiglitazone treatment in normotensive type 2 diabetic patients

Kemal Yasemin , Tutuncu Neslihan , Guvener Nilgun , Unal Asli , Atar Ilyas , Muderrisoglu Haldun

Objective: The thiazolidinediones are new and potentially useful developments in the treatment of type 2 diabetes and impaired glucose tolerance. We tested the effects of the thiazolidinedione, rosiglitazone on blood pressure in normotensive type 2 diabetes.Methods: After receiving approval from the local ethics committee, 25 normotensive diabetic patients were were enrolled to the study. Before the rosiglitazone treatment we measured plasma glucose, HbA...

ea0014p35 | (1) | ECE2007

Time dependent effects of rosiglitazone on heart and fluid dynamics: a 6-month follow up study

Kemal Yasemin , Demirag Nilgun , Tutuncu Neslihan , Yildirir Aylin , Atar Asli , Unal Asli

Objective: Thiazolidinediones (TZDs) have become a powerful tool for lowering insulin resistance. The problem of cardiovascular adverse events including fluid retention and risk of heart failure, although of a low incidence, should be well known and recognized. We aimed to evaluate the effects of rosiglitazone treatment on cardiac function and show whether these effects are reversible when we continued this treatment.Methods: Fourty-six type 2 diabetic p...

ea0014p36 | (1) | ECE2007

Peculiarities of heart rate control in patients with non-insulin dependent diabetes mellitus and hypertension

Vitols Andris , Voita Daina , Vitola Mara

Objective: To assess the sensitivity of exercise induced heart rate (HR) and baroreceptor reflex (BR) chronotrope reaction and HR variability for an early detection of autonomic nervous system impairment in non-insulin dependent diabetes mellitus (NIDDM) patients with arterial hypertension.Design and Methods: On 25 NIDDM pts (group A, 63±1.8 yrs. aged men, HbA1C 10.2±0.9%), 17 essential hypertension (EH) pts without glucose metabolis...

ea0014p37 | (1) | ECE2007

One injection of Detemir insulin administered before the lunch improves the metabolic control in type 1 diabetic patients

Ferrer-García Juan Carlos , Merino-Torres Juan Francisco , Segovia-Portolés Raquel , Campos-Alborg Vicente , Ballester Agustín Herrera , Sellés Francisco Piñón , Juan Carlos Sánchez

Objective: To compare 2 modalities of bolus-basal insulin therapy with aspart-detemir, according to the moment of administration of detemir (DET), before the lunch or bed-time, in type 1 diabetic patients with poor metabolic control.Methods: We conducted a prospective study of 40 type 1 diabetic patients, with poor metabolic control (HbAc 7–12%), randomized to receive treatment with 1 injection of DET before the lunch or bed-time and followed-up dur...

ea0014p38 | (1) | ECE2007

Oral antibodies to insulin receptor are found effective in the treatment of streptozotocin-induced diabetes in rats

Dugina Julia , Martyushev-Poklad Andrey , Kheyfets Irina , Spasov Alexander , Samokhina Maria , Epstein Oleg , Sergeeva Svetlana

An experimental study was designed to test a drug candidate for the treatment of diabetes mellitus in rats with streptozotocin (STZ) diabetes.Diabetes was induced in outbred male rats (280–300 g) by single iv injection of streptozotocin 50 mg/kg. The animals showing hyperglycemia (12–15 mM) 72 hours after injection were randomized to receive daily intragastric doses of distilled water, glibenclamid 8 mg/kg, or polyclonal antibodies to C-termina...

ea0014p39 | (1) | ECE2007

1 year endurance training at the level of the ventilatory threshold in type-2 diabetics reduces by 50% healh costs: a controlled randomized trial

Jean-Frédéric Brun , Audrey Jaussent , Marie-Louise Picot , Jacques Mercier , Christian Oréfaut

This trial was undertaken in order to evaluate the effects of endurance training on health cost in type 2 diabetes. 35 diabetic patients were randomly assigned to 2 groups: After 10 drop-outs, 15 followed a training program (8 sessions followed by training at home at the level of the ventilatory threshold VT) while 10 had only routine treatment. Both groups were followed over 1 year with evaluation at 30, 120, 240 and 365 days for health costs, blood pressure, and a...

ea0014p40 | (1) | ECE2007

PED levels are increased in peripheral blood leucocytes from euglycaemic subjects at-risk of type 2 diabetes

Valentino Rossella , Lupoli Gelsy Arianna , Formisano Pietro , Esposito Iolanda , Alberobello Anna Teresa , Perfetti Anna , Cirillo Michele , Gennarelli Nicola , Gargiulo Giovanna , Lobello Roberto , Beguinot Francesco

Background: Phosphoprotein enriched in diabetes (PED) is a scaffold protein widely produced in different tissues; it is involved in multiple cellular functions, including insulin-regulated glucose transport. Previous findings showed that in individuals with type 2 diabetes (T2D) the PED gene is overexpressed in skeletal muscle (SM) and adipose tissue (AT), both target tissues for insulin activity. Our group has recently evidenced that PED protein is also expressed in periphera...

ea0014p41 | (1) | ECE2007

Effectiveness of α-lipoic acid in prevention of peripheral diabetic neuropathy

Jikurauli Nino , Giorgadze Ellen , Svani Nino , Chachibaia Vasil , Bestavashvili Felix , Lominadze Tea , Chanturia Nia , Malazonia Anna

Background: The aim of our study was to assess the effectiveness of α-lipoic acid for the reducing the risk of developing peripheral diabetic neuropathy.Subjects and methods: We have studied 38 patients with type I and II diabetes, from age 28 to 35. The mean duration of disease was 9 years. Patients were divided in two groups. In group I were included 15 patients which got α-lipoic acid in order to prevent peripheral diabetic neuropathy in dos...

ea0014p42 | (1) | ECE2007

Adhesion molecules s-VCAM-1 and s-ICAM-1 in members of families with familial combined hyperlipidemia

Karásek David , Vaverková Helena , Halenka Milan , Fryšák Zdenek , Novotný Dalibor , Budíková Marie

Objective: Familial combined hyperlipidemia (FCH) is the most common familial hyperlipidemia with a high risk of the early atherosclerosis. The aim of this study was to compare levels of s-ICAM-1 and s-VCAM-1 in asymptomatic members of FCH families with healthy controls and to find out relation between s-ICAM-1, respective s-VCAM-1, and risk factors accompanying FCH. We also investigate association between adhesion molecules and intima-media thickness of common carotid artery ...

ea0014p43 | (1) | ECE2007

Body fat distribution, lipid and adipokine levels in South African type 2 diabetic patients of African and Indian origin

Waisberg Rita , Paiker Janice , George Jaya , Crowther Nigel

Introduction: The increased insulin resistance seen in Type 2 diabetic patients has been shown to be associated with abdominal fat accumulation, hypertension, and dyslipidaemia. The dyslipidaemia is characterized by raised serum triglycerides and decreased high density lipoprotein cholesterol (HDL) levels. The aim of the present study was to investigate the relationship between abdominal fat, lipid and adipokine secretion in South African type 2 diabetic patients of Indian and...

ea0014p44 | (1) | ECE2007

Low dose cyclosporin and methotrexate administration induces remission of Type 1 diabetes mellitus

Sobel Douglas , Abbassi Val , Henzke Annette

Although, high doses of cyclosporine (cyclo)has been demonstrated to inhibit the development of type 1diabetes mellitus (T1D), its usefulness was limited by its toxicity. Since methotrexate (Mtx) and cyclo have been shown to synergistically act in other disease processes, we determined if low dose cyclo and Mtx therapy could inhibit the development of diabetes and reduce or eliminate the need for insulin therapy in a pilot study.Methods: Insulin dose and...

ea0014p45 | (1) | ECE2007

Progression of diabetic retinopathy in pregestational diabetes mellitus

Guerrero R , Martinez Brocca MA , Pomares MA , Palma S , Gonzalez I , Acosta D , Moreno E , Villamil F

Background: Pregnancy may adversely affect the progression of diabetic retinopathy and can have serious implications for the pregnant women.Aim: To asses the impact of pregnancy on the progression of diabetic retinopathy in women with type 1 and type 2 diabetes mellitus and to identify risk factors for the progression of retinopathy during pregnancy.Methods: 306 diabetic women, 229 (75.4%) with type 1 and 77 (23.9%) with type 2 di...

ea0014p46 | (1) | ECE2007

Comparison of insulin monotherapy and combination therapy with insulin and metformin or insulin and rosiglitazone or insulin and acorbose in type 2 diabetes

Yilmaz Hamiyet , Gursoy Alptekin , Sahin Mustafa , Tutuncu Neslihan Bascil , Demirag Nilgun Guvener

The aim of this study was to compare the efficacy of treatment with insulin alone, insulin plus acarbose, insulin plus metformin or insulin plus rosiglitazone in type 2 diabetic subjects who were previously on insulin monotherapy, and to evaluate the effects of these treatments on cardiovascular risk factors including lipid profile, C-reactive protein (CRP) and fibrinogen. Sixty-six poorly controlled type 2 diabetic patients on insulin monotherapy were involved. They were rand...

ea0014p47 | (1) | ECE2007

Competition between catecholamines and glucose for binding sites on proteins of erythrocytes

Lepschy Michael , Moestl Erich , Palme Rupert

Glucose is slowly linked to haemoglobin in a non-enzymatic reaction and the determination of a glycated protein (HbA1c) is used for long term monitoring blood glucose concentrations. Radiometabolism studies in sheep showed that a haemoglobin-adduct formation also takes place with epinephrine or norepinephrine.The aim of our study was to elucidate if there is a competition between catecholamines and glucose for binding sites on proteins of the erythrocyte...

ea0014p48 | (1) | ECE2007

Body composition, emotional state and quality of life in patients with diabetes mellitus type 2

Lasaite Lina , Lasiene Jurate

Diabetes mellitus type 2 (DM2) is affecting physical and psychological health.Objective: Compare anthropometric data, body composition, lipids levels, emotional state, quality of life (QoL) of DM2 patients and that of healthy persons of the same age.39 persons (58.1±9.6 years) with DM2 (18 male, 21 female) and 41 healthy persons (54.3±9.9 years) (22 male, 19 female). Profile of Mood State (POMS) used for emotional state e...

ea0014p49 | (1) | ECE2007

Deleterious effects of beta-blockers on arterial stiffness and central pulse pressure in menopausal women: baseline findings from the Cashmere trial

Laurent S , Christin Maitre S , Gompel A , Boutouyrie P , Jaillon P , Thuilliez Ch , Zannad F , Pornel B , Pannier B , Struijker-Boudier H , Van Bortel L , Kuntz Ch , Keanry-Schwartz A , Pithois-Merli I , Simonneau D , Simon T

Beta-blockers (BB) may be less effective than other antihypertensive drugs for stroke prevention in patients with primary hypertension (ASCOT and LIFE studies). Our study compares arterial stiffness and central PP between users (BB+) and non users of BB (BB-), among menopausal women with hypercholesterolemia and no history of CV disease.Methods and Results: We used the baseline data of 664 menopausal women, screened for the Cashmere study, a12-month doub...

ea0014p50 | (1) | ECE2007

Prevalence of GADA and IAA in elderly patients with type 2 diabetes

Kurowska Maria , Tarach Jerzy S , Jankowska Helena , Kowalczyk Mariusz , Malicka Joanna , Nowakowski Andrzej

Little is known about the prevalence and significance of islet cell immunity in elderly patients with type 2 diabetes. The low antibody titers against islet-cell antigens in LADA elderly patients may be a sign of a less aggressive autoimmune diabetes.The objective: To establish the changing frequency and titers of GADA and IAA in elderly diabetics.Material: 83(56F;27M)diabetic patients (60–91 y) divided in age related groups. ...

ea0014p51 | (1) | ECE2007

Comparison of plasma homocysteine concentrations (HYC) in patients with acute coronary syndrome (ACS) and newly or previously diagnosed type 2 diabetes

Kurowska Maria , Tarach Jerzy S , Gernand Wojciech , Kudlicki Janusz , Tarkowski Adam , Nowakowski Andrzej , Czekajska Teresa , Solski Janusz

Introduction: The patients with ACS and scheduled for an elective coronary angiography have high frequency of both newly and previously diagnosed diabetes. The diabetic patients with acute myocardial infarction have an increased risk of death. Elevated blood HYC is strongly related to an increased risk for atherosclerosis and cardiovascular disease. This association is particularly evident in patients with diabetes.Aim of the study: An attempt to evaluat...

ea0014p52 | (1) | ECE2007

The association between carotid artery intima-media thickness and cardiovascular mortality and morbidity in Type 2 diabetes: a retrospective study

Ataoglu Hayriye Esra , Yenigun Mustafa , Saler Tayyibe , Yigit Zehra , Temiz Levent , Saglam Zuhal , Çetin Faik , Kumbasar Baki , Sar Fuat , Ahbap Suleyman

Carotid artery intima-media thickness (CCA-IMT) highly correlates with cardiovascular events in type 2 diabetes (T2DM). We aimed to determine the cardiovascular mortality and morbidity incidence regarding CCA-IMT and Framingham Score compared with preceding results of T2DM individuals. Our aim was to determine whether ultrasonographic evaluation of carotid arteries may predict cardiovascular mortality, morbidity and diabetic complications in T2DM patients.<p class="abstext...

ea0014p53 | (1) | ECE2007

Comparison of the effects of gliclazide and glibenclamide on insulin resistance and metabolic parameters

Gokturk Rabia , Ataoglu Hayriye Esra , Yenigun Mustafa , Ahbap Suleyman , Saglam Zuhal , Temiz Levent , Çetin Faik , Sar Fuat , Kumbasar Baki

Introduction: It has been shown with many studies that sulphonylureas may have a negative effect on parameters of insulin resistance while improving glucose regulation. However not all of the sulphonylureas have the same effect. This analysis assessed the different effects of sulphonylureas on some metabolic parameters of insulin resistance.Method: Newly diagnosed 25 T2DM individuals who were naive of oral antidiabetic therapy were recruited and randomiz...

ea0014p54 | (1) | ECE2007

24-hour ambulatory blood pressure and aortic dimensions in women with Turner syndrome

Hjerrild Britta , Sorensen Keld , Pedersen Erik Morre , Lundorf Erik , Horlyck Arne , Christiansen Jens Sandahl , Gravholt Claus Hojbjerg

Study objective: To study blood pressure (BP) levels and aortic dimensions in women with Turner syndrome (TS).Materials and methods: 102 women with TS (mean age 37.7 years; 18–62 years). 24 hour ambulatory BP measurement and echocardiography was performed on participants.Results: Mean BP systolic (sys) and diastolic (dia) values were (±SD): sysBPday 128.0±15.3; diaBPday 81.6±11.8; sysBPnight 110.4...

ea0014p55 | (1) | ECE2007

Plasma marker of lipid peroxidation and type 2 diabetes in subject with coronary artery disease in Iranian subjects

Haghparast Fariborz , Nourooz-Zadeh Jaffar

Objective: Abnormal lipid profile is an important risk factor in the development of macrovascular atherosclerotic complications in patients with type 2 diabetes mellitus (T2D). The aim of this study was to investigate the relationship between lipid profile and lipid peroxidation in type 2 diabetics with and without coronary artery disease (CAD).Materials and methods: We studied 80 patients with T2D, 40 with CAD and 40 without CAD. We also studied 50 non-...

ea0014p57 | (1) | ECE2007

Effect of testosterone replacement therapy on adipocytokines in hypogonadal men with Type 2 diabetes

Kapoor D , Clarke S , Stanworth R , Channer KS , Jones TH

Serum testosterone level is known to inversely correlate with insulin sensitivity and obesity in men. Furthermore, there is evidence to suggest that testosterone replacement therapy reduces insulin resistance and visceral adiposity in Type 2 diabetic men. Adipocytokines are hormones secreted by adipose tissue and contribute to insulin resistance. We report a double-blind placebo controlled crossover study in 20 hypogonadal Type 2 diabetic men examining the effect of testostero...

ea0014p57 (1) | (1) | ECE2007

Effect of testosterone replacement therapy on adipocytokines in hypogonadal men with Type 2 diabetes

Kapoor D , Clarke S , Stanworth R , Channer KS , Jones TH

Serum testosterone level is known to inversely correlate with insulin sensitivity and obesity in men. Furthermore, there is evidence to suggest that testosterone replacement therapy reduces insulin resistance and visceral adiposity in Type 2 diabetic men. Adipocytokines are hormones secreted by adipose tissue and contribute to insulin resistance. We report a double-blind placebo controlled crossover study in 20 hypogonadal Type 2 diabetic men examining the effect of testostero...

ea0014p58 | (1) | ECE2007

A role of the liver in the infringements of lipid metabolism of patients with diabetes mellitus type 2 and metabolic syndrome

Korpachev Vadim , Kovalchuk Alla , Kushnareva Natalija , Kovalchuk Volodymyr

Different interdependences with symptoms of insulin resistance give us the possibility to consider steatosis as a disorder of the liver with metabolic syndrome (MS).The aim of study is to assess the role cholesterol-HDL in rise of diabetic steatohepatosis.40 patients with Diabetes Mellitus type 2 (DM) and signs of MS were examined to determine spreading of steatohepatosis as one of the factors of insulin resistance. Only 8 of them ...

ea0014p59 | (1) | ECE2007

Radionuclide study of hepatobiliary system function in patients with type 2 diabetes and metabolic syndrome

Slavnov Valentin , Kovalchuk Alla , Korpachev Vadim , Kushnareva Natalija

The aim of the research is to study the functional state of liver parenchyma in patients with type 2 diabetes and to analyze hepatobiliary system disorders depending on marker of metabolic syndrome (MS).The study involved 22 patients with type 2 diabetes and MS and 8 healthy persons. Dynamic hepatoholecystoscintigraphy was performed using RKC 301T gamma camera after Tc-99m mesida administration and bile-expelling meal.We establishe...

ea0014p60 | (1) | ECE2007

Omega-3 polyunsaturated fatty acids in the treatment of cardiovascular autonomic neuropathy in Type 2 diabetes mellitus patients

Serhiyenko Victoria , Serhiyenko Alexander , Serhiyenko Ludmila

Background and aims: The aim of this study was to assess the effects of docosahexaenoic (DHA) and eicosapentaenoic acid (EPA) on the heart rate variability (HRV), some biochemical parameters in patients (pts) with Type 2 diabetes mellitus and cardiovascular aunonomic neuropathy (CAN).Materials and methods: 39 pts with CAN (54±5 yrs) were allocated in two groups: A (n=26) were receiving capsules of fish oil every day (2.0 g EPA, 2.0 g DHA and ...

ea0014p61 | (1) | ECE2007

Nicotinamide and alpha-lipoic acid in the treatment of cardiovascular autonomic neuropathy in Type 2 diabetic patients

Serhiyenko Ludmila , Serhiyenko Alexander , Serhiyenko Victoria

Background and aims: The present study has examined the effect of α-lipoic acid (ALA) and nicotinamide (NA) on the heart rate variability (HRV), superoxide dismutase (SOD), gluthation peroxidase (GPO), catalase activities, reduced gluthatione (GSH), malondialdegide (MDA) contents in the RBCs’ in Type 2 diabetic patients (T2DM) with cardiovascular autonomic neuropathy (CAN).Materials and methods: 59 patients with T2DM and CAN (59.3±7.9 year...

ea0014p62 | (1) | ECE2007

Plasma measures of oxidative stress and antioxidant status in type 2 diabetes mellitus

Haghparast Fariborz , Nourooz-Zadeh Jaffar

Objective: The aim of this study was to test the hypothesis that type 2 diabetes mellitus is associated with increased oxidative stress in Iranian subjects.Materials and methods: The study population consisted of Fifty-nine patients with type 2 diabetes (mean age 62.5±8.7 years). Type 2 diabetes was diagnosed according to the American Diabetes Association criteria. 36 patients had diabetes complications and 23 patients had no complications. For the ...

ea0014p63 | (1) | ECE2007

Does screening of primary hyperaldosteronism only lead to diagnosis of more adrenal hyperplasia?

Sigurjonsdottir Helga , Gronowitz Mikael , Anderson Ove , Eggertsen Robert , Herlitz Hans , Johannsson Gudmundur

Background: The prevalence of primary hyperaldosteronism (PA) has recently been reported as high as 7.5% in patients with hypertension from unselected populations (USP) and up to 22% in selected populations (SP). Whether increased screening and diagnosis of PA will lead to more findings of curable PA (aldosterone producing adenoma, APA) is unclear.Methods: Three-hundred fifty-three consecutive patients with hypertension, age 20 to 88 years were included ...

ea0014p64 | (1) | ECE2007

Liquorice in moderate doses decreases serum levels of vitamin B12 but does not affect the serum lipid levels

Sigurjonsdottir Helga , Arnadottir Margret , Wallerstedt Sven

Background: Liquorice in moderate doses increases blood pressure (BP) in healthy individuals (NT) as well as patients with hypertension (HT) due to increased cortisol effect. Glycyrrhetinic acid, the active substance in liquorice, inhibits 11beta-hydroxysteroid-dehydrogenase type 2 (11betaHSD2) which converts the active hormone cortisol to the inactive hormone cortisone. Recently it has been reported that treatment with glucocorticoids decreases serum levels of cobalamin (B12)...

ea0014p65 | (1) | ECE2007

Decreased insulin sensitivity in young lean hypertensive men is not associated with increased visceral fat and changes in plasma adipocytokines

Penesova Adela , Radikova Zofia , Cizmarova Eva , Belan Vitazoslav , Vigas Milan , Koska Juraj

Objective: Increased abdominal visceral adipose tissue (VAT) deposition is associated with insulin resistance in obese and/or hypertensive patients. We investigated the association of insulin sensitivity with the amount of VAT in young, lean, non-treated males with recently established high normal blood pressure or hypertension grade 1 (HT).Subjects and methods: Twenty-one subjects with HT (age 20.3±0.6 years, BMI 22.4±0.5 kg/m2, sys...

ea0014p66 | (1) | ECE2007

Comparison of twice daily NPH insulin versus once daily glargine insulin in the frequency of nocturnal hypoglycemia in Type2 diabetic patients with congestive heart failure

Kurtulmus Neslihan , Demirdogen Fatma , Tukek Tufan

Aim: We had the aim to determine the insulin treatment strategy that could prevent or decrease the occurrence of hypoglycaemia while providing better regulation of blood glucose in Tip 2 diabetic patients with cardiac failure.Method: The patients demonstrating similar characteristics with respect to the age, body mass index, the duration of diabetes and heart failure were randomized into two groups as insulin glargine (n: 19) and NPH (n: 11...

ea0014p67 | (1) | ECE2007

Intravenous constant ghrelin infusion in healthy young men: sustained cardiovascular effects of supraphysiological ghrelin levels

Vestergaard Esben Thyssen , Andersen Niels Holmark , Hansen Troels Krarup , Rasmussen Lars Melholt , Moller Niels , Sorensen Keld , Sloth Erik , Christiansen Jens Sandahl , Jorgensen Jens Otto Lunde

Objective: The short-term cardiovascular effects of continuous ghrelin infusion in healthy humans remain to be studied.Methods: Fifteen healthy, young and normal-weight men volunteered to participate in a randomized double-blind, placebo-controlled cross-over study. The local ethics committee approved the study. We used a constant infusion of human ghrelin at a rate of 5 pmol/kg body weight per minute for 180 minutes and measured peak left ventricular my...

ea0014p68 | (1) | ECE2007

Circulating retinol binding protein 4 and protein C inhibitor are not related to insulin resistance

Promintzer Miriam , Krebs Michael , Luger Anton , Bischof Martin Georg , Nowotny Peter , Binder Christoph , Esterbauer Harald , Anderwald Christian

Recent data suggest that circulating retinol binding protein-4 (RBP4) is involved in the pathogenesis of insulin resistance in rodents and humans. Moreover, protein C inhibitor (PCI) which specifically binds retinoic acid was found to be increased in myocardial infarction survivors who are also insulin-resistant.Therefore, we investigated the association of insulin resistance with plasma retinol binding factors (RBP4 and PCI active antigen) in nondiabeti...

ea0014p69 | (1) | ECE2007

KCNJ11 and ABCC8 promotor variants in congenital hyperinsulinism

Brusgaard Klaus , Hussain Khalid , Svargo Lone , Brock-Jacobsen Bendt , Christesen Henrik

Congenital hypoglycemic hyperinsulinemia (CHI) is a clinical and genetic heterogeneous entity. Clinical manifestations can vary from serious life threatening to milder difficultly identifiable cases. Children who don’t react adequate to medical treatment are subject to pancreatic recession. The molecular ethiology are from recessive mutations of the ABCC8 (SUR1) and KCNJ11 (Kir6.2) to dominant mutations of the GCK or GDH genes. Focal dysplasia ...

ea0014p70 | (1) | ECE2007

Effect of L- carnitine supplementation on glycemic profile in patients with type 2 diabetes mellitus

Sakerhosseini Rahebeh , Rahbar Alireza , Saadat Avid , Pordal Amir hamzeh , Taleban Feroug azam , Golestan Banefsheh

Background: It has been thought that L-carnitine is effective in improving insulin-mediated glucose disposal either in healthy subjects or in type 2 diabetic patients,and carnitine plays an important role in diabetes mellitus omplications (cardiovascular disease).Objective: We designed this study to investigate the effects of oral L-carnitin administration on fasting plasma glucose (FPG) and glycosylated hemoglob (HbA1c), in patients with diabetes mellit...

ea0014p71 | (1) | ECE2007

Raised serum, adipocyte and adipose tissue retinol binding protein 4 (RBP4) in women with polycystic ovary syndrome

Tan Bee Kang , Chen Jing , Pua Sheryl , Richard Kennedy C , Randeva Harpal S

Context: Polycystic ovary syndrome (PCOS) is associated with insulin resistance (IR) and obesity, both predisposing factors to type 2 diabetes. A very recently described adipokine, retinol-binding protein 4 (RBP-4), has been shown to modulate insulin signalling and possibly lead to IR. At present, there is no data that depict the relative expression of RBP-4 in either serum or adipose tissue of PCOS women.Objectives: In women with PCOS compared to matche...

ea0014p72 | (1) | ECE2007

Polymorphisms of von Willebrand factor gene promoter modulate the corticosteroid-mediated increase of VWF levels in Cushing’s syndrome.

Daidone Viviana , Sartorello Francesca , Albiger Nora , Mantero Franco , Pagnan Antonio , Casonato Alessandra , Scaroni Carla

Cushing’s syndrome (CS) is associated with hypercoagulable state, mainly dependent on corticosteroid-induced increase of von Willebrand factor (VWF) levels, even though this does not affects all patients. In normals plasma VWF levels are genetically determined by ABO blood groups and polymorphisms G/C −1793, C/T −1234, A/G −1185, G/A −1050 of VWF promoter. These SNPs segregate as haplotype 1 (G/C/A/G) and haplotype 2 (C/T/G/A) with genotype 1/1 (GG...

ea0014p73 | (1) | ECE2007

Circulating pro- and anti-inflammatory cytokines in women with gestational diabetes

Kuzmicki Mariusz , Telejko Beata , Zonenberg Anna , Szamatowicz Jacek , Kretowski Adam , Nikolajuk Agnieszka , Gorska Maria

Gestational diabetes mellitus (GDM) identifies a population of women at high risk of subsequent type 2 diabetes mellitus, representing an early stage in the natural history of the disease. Systemic inflammation is associated with the development of type 2 diabetes but the data concerning pro- and anti-inflammatory cytokines in patients with GDM are limited. The aim of our study was to investigate serum concentrations of interleukin-8 (IL-8), IL-18 and IL-10 in pregnant women w...

ea0014p75 | (1) | ECE2007

Metabolic improvement in diabetic patients with glucose continuous monitoritation in real time

Morales Portillo Cristobal , Serrano Olmedo Isabel , Maraver Silvia , Fernandez Arguelles Amaya , Garcia-Quiros Muñoz Juan Manuel , Martinez De Pinillos Guillermo , De La Cuesta Mayor Carmen , Sendon Perez Angel

Objective: To study if the use of continuous glucose monitoritation systems in real time (GUARDIAN REAL TIME®) in the diabetic patients could improve the metabolic control.Material and method: 15 type 1diabetic patients studied (age: 33.8±13.40, years of evolution: 17.06±11.32) in intensive treatment (5 multidoses, 10 insulin pump) which were made one first blind 4 days long monitoritation to them, PERIOD 1 (P. BLIND) with senso...

ea0014p76 | (1) | ECE2007

The role of stress related aldosterone secretion in essential hypertension

Markou Athina , Androulakis Ioannis , Gouli Aggeliki , Dimitriou Kostantina , Zacharoulis Achilleas , Papadopoulos Kostantinos , Barlogiannis Theodoros , Chariatis Evangelos , Halapas Antony , Kourlaba Eleni , Ragkou Despoina , Kondyli Georgia , Piaditis George

Introduction: Approximately 90% of the hypertensive population is characterized as having essential hypertension. Apart from renin and K+, ACTH plays an important role in aldosterone secretion, being a potent stimulant under situations of stress. Up to date, the association between stress and aldosterone-related hypertension has not been studied and that is the aim of our study.Methods: 36 hypertensive patients and 14 matched for age and sex c...

ea0014p77 | (1) | ECE2007

Dialysis therapy and its complications

Bublik Evgeniya , Galstyan Gagik , Melnichenko Galina , Safonov Victor , Shutov Euginy , Filipcev Pavel , Udovichenko Oleg

Aim: To study prevalence of foot complications in patients on dialysis therapy and evaluate the role the haemodynamic changes during dialysis procedure in development of foot problems.Methods: 109 dialysis patients, mean age 49 years. 60 of them had diabetes mellitus (DM): 29 on haemodialysis (HD), 31 on peritoneal dialysis (PD). Non-diabetic patients (NDM): 24 HD, 25 PD.Vascular status: doppler, photoplethysmography. Polyneuropath...

ea0014p78 | (1) | ECE2007

The effect of interferon treatment on glucose metabolism in patients with chronic hepatitis

Alev Atilla , Kubat Uzum Ayse , Murat Tatli Ali , Oner Kaya Fatih , Dik Iskender , Bedir Burhan

Introduction: In recent years, interferon (IFN) is used in treatment of chronic hepatitis and the studies about the side effects of IFN therapy are increasing.Objective: We aimed to investigate the effects of IFN therapy on glucose metabolism.Materials and methods: Study group was consisted with 30 patients who were diagnosed as chronic hepatitis. Sixteen of 30 were chronic hepatitis B and 14 were chronic hepatitis C. Diagnose was ...

ea0014p79 | (1) | ECE2007

Unacylated ghrelin (UAG) enhances the early insulin response to meal, improves glucose metabolism and decreases free fatty acids levels in healthy volunteers

Prodam Flavia , Riganti Fabrizio , Gramaglia Elena , Benso Andrea , Lucatello Barbara , Broglio Fabio , Abribat Thierry , Jan van der Lely Aart , Ghigo Ezio

Ghrelin circulates in two different forms. Acylated ghrelin (AG), a natural ligand of the GH Secretagogue receptor (GHS-R) type 1a, exerts several biologic central and peripheral actions including stimulation of GH secretion, but also modulation of insulin secretion, glucose and lipid metabolism. Unacylated ghrelin (UAG), despite unable to bind the GHS-R1a, is biologically active showing some influence in vitro and in vivo on glucose and lipid metabolism likely m...

ea0014p80 | (1) | ECE2007

Weight-related concentrations of steroid hormones in patients of both sexes with preserved gonadal function suffering from coronary artery disease

Sablik Zbigniew , Samborska-Sablik Anna , Henryk Goch Jan , Kula Krzysztof

Aim: To define concentrations (conc) of steroid hormones in patients (pts) with preserved gonadal function, suffering from coronary artery disease (CAD), and to assess their relations to patients’ weight.Material: Pts with coronarographically proved CAD: C-W group (gr)-52 women (W) in the age 43±3 years, with stable menstrual rhythm, C-M gr-46 men (M) in the age 52±6 years. Healthy volunteers: H-W gr-15 W (H-W) in the age 41±4 years, ...

ea0014p81 | (1) | ECE2007

Diabetic patient’s evaluation of continuous glucose monitoring sensors versus capillary glucose measurements

De Pinillos Gordillo Guilermo Martínez , Fernández-Argüelles Amaya , Garcí-Quirós Jm Juan Manuel

Objective: To evaluate the monitoritation systems acceptance: capillary glucose measurements and continuous glucose sensors (CGSM and GUARDIAN).Research design and methods: 15 diabetics patients were monitoritatied in two diferents periods of time. (Period 1: Guardian, 86 hours long. Period 2: CGSM 72 hours long). Later, they had to fill a satisfaction questionnaire concerning several aspects which were valued from 0–6.<table boarder="1" cellpad...

ea0014p82 | (1) | ECE2007

Dehydroepiandrosterone therapy in men with verified coronary heart disease: the effects on fibrinogen, plasminogen activator inhibitor-1 (PAI-1) and tissue plasminogen activator (tPA)

Rabijewski Michal , Papierska Lucyna , Zgliczynski Wojciech

Objectives: The aim of this study was to analyze the influence of DHEA therapy on fibrinogen, plasminogen activator inhibitor-1 (PAI-1) and tissue plasminogen activator (tPA) plasma concentrations in men with decreased serum DHEAS levels and angiographically verified coronary heart disease (CHD).Material and methods: The study included thirty men aged 41–60 years (mean age 52±0.90 yr) with serum DHEAS concentration <2000 μg/l, who were...

ea0014p83 | (1) | ECE2007

Insulin resistance and insulin secretion in non-diabetic acromegalic patients

Niculescu Dan , Purice Mariana , Lichiardopol Radu , Coculescu Mihail

Aim: To set up, in acromegaly without diabetes mellitus, a correlation between the disease activity in GH-secreting adenoma (AA) - assessed by minimum GH serum level during an oral glucose tolerance test (OGTT) - and severity of insulin resistance (IR), assessed by HOMA-IR index.Methods: 75 out of 88 consecutive patients with acromegaly hospitalized in our department were included in this study. 13 patients proved to have diabetes mellitus and were exclu...

ea0014p84 | (1) | ECE2007

Response to metformin treatment in adolescent siblings with familial partial lipodystrophy of the dunnigan variety (FPLD) due to the R482W LMNA gene mutation

Ryan James , Kiely Patrick , Crowley Vivion , Maher Michael , O’Connor Rosemary , O’Halloran Domhnaill

FPLD is a rare monogenic cause of insulin resistance. We document responses to treatment with metformin in 2 adolescent sisters with FPLD due to heterozygosity for R482W LMNA gene mutation.The probands, aged 14 and 16 years, presented with secondary amenorrhoea, hirsutism and progressive acanthosis nigricans. Phenotypically they showed central obesity, nuchal enlargement, and thin muscular arms. These changes occurred post-pubertally. Anthrpometri...

ea0014p85 | (1) | ECE2007

Insulin sensitivity and lipid levels in pateints with primary hyperparathyroidism

Cvijovic Goran , Micic Dragan , Kendereski Aleksandra , Zoric Svetlana , Sumarac-Dumanovic Mirjana , Pejkovic Danica , Georgiev Maja

Patients with primary hyperparathyroidism (PHPT) are insulin resistant. The effect of PHPT on lipid levels and low-grade inflammation leves is unknown. The aim of our study was to estimate the cardiovascular risk profile in patients with PHPT. Methods: In patients with PHPT (N=19; age: 58.15±8.38 years; PTH 180.83±104.15 ng/l, calcium 2.97±0.19 mmol/l) insulin sensitivity (measured using euglycemic hyperinsulinemic clamp - M value), lipids (total choleste...

ea0014p86 | (1) | ECE2007

The effect of surgical treatment on insulin sensitivity in pateints with primary hyperparathyroidism

Kendereski Aleksandra , Micic Dragan , Cvijovic Goran , Zoric Svetlana , Sumarac-Dumanovic Mirjana , Pejkovic Danica , Georgiev Maja

It was reported that patients with primary hyperparathyroidism (PHPT) are insulin resistant, and that surgical treatment may improve insulin sensitivity in this group of patients. The aim of our study was to evaluate the effect of surgical treatment on insulin sensitivity in patients with PHPT. Methods: In patients with PHPT (N=19; age: 58.15±8.38 years) insulin sensitivity was estimated using euglycemic hyperinsulinemic clamp (M value) before and 3 months after su...

ea0014p87 | (1) | ECE2007

Insulin-sensitivity and glycaemic control improve on rosuvastatin (RSV) treatment in hypertriglyceridaemic type-2 diabetes (T2DM)

Silvani Giorgio , Tartaglia Andreas , Acquati Silvia , Vacirca Anna Laura , Bondi Antonella , Gagliardi Lorenza , Nizzoli Maurizio

Aims: Studies on statins and insulin-sensitivity in T2DM are highly controversial. We aimed to evaluate the effect of RSV in type-2 diabetic people and whether its action may be phenotype-dependent, i.e. triglyceride (TG)-related (study approved by the local Ethical Committee).Methods: 48 type-2 diabetic pts (22 M:26 F), in a poor glycaemic control with oral agents, insulin or a combination therapy (unchanged over the study), were given RSV 10 mg for 12 ...

ea0014p88 | (1) | ECE2007

Gestational diabetes mellitus and adiponectin levels

Culha Cavit , Gorar Suheyla , Serter Rustu , Demir Yavuz , Karakaya Pinar , Aral Yalcin

Aim: Hypoadiponectinemia is known to be associated with insulin resistance, diabetes and obesity. Since gestational diabetes is together with increased body mass index (BMI) and decreased insulin sensitivity, the evaluation of adiponectin levels in these patients is interesting. We investigated the relationship between adiponectin and glucose tolerance during pregnancy and after delivery.Materials and methods: We evaluated plasma adiponectin levels, insu...

ea0014p89 | (1) | ECE2007

Lipoprotein Lp(a) in patients with systemic lupus erythematosus. Relationship with disease activity and anticardiolipin antibodies

Athanassiou Panagiotis , Kostoglou-Athanassiou Ifigenia , Papadopoulou Despina , Galanaki Chrysoula , Kalogirou Thomais , Kordalis Athanasios , Antoniadis Christodoulos , Kaldrymidis Philippos

Systemic lupus erythematosus (SLE) is a multisystem multifactorial autoimmune disorder. The survival of SLE patients has been improved by the administration of immunomodulatory therapy. Patients, however, are affected by late onset complications of the disease such as atherosclerosis. Lipoprotein Lp(a) is a known risk factor for the development of atherosclerosis.The aim was to study Lp(a) levels and their relationship with disease activity in SLE patien...

ea0014p90 | (1) | ECE2007

Lipoprotein Lp(a) in patients with rheumatoid arthritis and its relationship with disease activity

Athanassiou Panagiotis , Kostoglou-Athanassiou Ifigenia , Papadopoulou Despina , Galanaki Chrysoula , Kalogirou Thomais , Kordalis Athanasios , Antoniadis Christodoulos , Kaldrymidis Philippos

Epidemiological studies indicate that rheumatoid arthritis (RA) patients have increased mortality. Cardiovascular disease seems to be one of the major causes of death in patients with RA. Lipoprotein disorders are observed in patients with systemic autoimmune disorders as well as in patients with RA. Lipoprotein Lp(a) is an independent risk factor for the development of cardiovascular disease.The aim of the study was estimate lipoprotein Lp(a) levels and...

ea0014p91 | (1) | ECE2007

Impaired proinsulin secretion before and during oral glucose stimulation in HIV-infected patients, who display fat redistribution

Haugaard Steen B , Andersen Ove , Halsall Ian , Iversen Johan , Nicholas Hales Charles , Madsbad Sten

Beta-cell function of HIV-infected patients on highly active antiretroviral therapy (HAART), who display lipodystrophy, may be impaired. An early defect in beta-cell function may be characterized by an increased secretion of 32–33 split proinsulin (SP) and intact proinsulin (IP).To address this issue the secretion pattern of SP and IP of 16 HIV-infected men with lipodystrophy (LIPO) and 15 HIV-infected men without lipodystrophy (NONLIPO) were studie...

ea0014p92 | (1) | ECE2007

Concentration of vasopressin and of N- terminated naturetic propeptyde type B – potent predictors of survival of patients after cardiac arrest

Samborska-Sablik Anna , Sablik Zbigniew , Gaszynski Wojciech , Henryk Goch Jan , Kula Krzysztof

Aim: To assess concentration (conc) of arginine vasopressin (AVP) and conc of N- terminated naturetic propeptyde type B (NTpBNP) in patients (pts) after cardiac arrest (CA) and their role for clinical state of pts after CA.Material: 52 pts after CA, 36 men and 16 women, in the age 62±13 years. CA was caused by ventricular fibrillation in 31 cases, by asystolia in 15 and by pulseless electrical activity in 6. 28 pts died after CA (P-CA-D), 24 survive...

ea0014p93 | (1) | ECE2007

Changes in plasma adiponectin during the treatment of diabetic ketoacidosis

Emre Yildirim A , Selim Demir Yavuz , Ozturk Abidin , Ozden Barazi A , Cetiner Hacer , Kilic Gokcen , Ozcan Tugrul , Acar Yasar , Demirbas Berrin , Gursoy Gul

Background: Low plasma adiponectin concentrations are associated with diabetes mellitus. Resuts from animal studies suggest that adiponectin plays an important role in regulating insulin action. Leptin levels found to be low in patients with diabetic ketoacidosis (DKA). The recent studies showed that insulin replacement during DKA increased leptin concentrations. In our study, we aimed to determine the effect of insulin replacement on serum leptin and adiponectin concentration...

ea0014p94 | (1) | ECE2007

Power spectral analysis (PSA) of heart rate variability (HRV) in the detection of cardiac autonomic neuropathy (CAN) in subjects with diabetes mellitus

Khamis Amjed , Rizvi SR , Hoashi Shu , Byrne Marie , Firth RG , Kinsley Brendan

Standard Autonomic Function tests AFT may not detect subclinical CAN. Modalities exist (Frequency Domain) using PSA of HRV, which may detect subclinical CAN.We performed standard AFTs (HR response to deep breathing, Valsalva, Tilt at 1 min and 6 min) and PSA of HRV on these tests in 46 subjects (29 DM and 17 Controls) matched for age and sex. We sought to establish if those DM subjects considered normal by AFT would exhibit abnormalities in PSA of HRV. W...

ea0014p95 | (1) | ECE2007

Glicoregulation in obese diabetics treated with glargin insulin in combination with metformin and with glargin in combination with glimepirid

Velija-Asimi Zelija

Aims: The aim of study was to evaluate glucose control in obese diabetics during six months of treatment with glargin insulin in combination with metformin and glimepirid.Methods: In beginning of study excluded patients who had coronary heart and kidneys disease before. In study included 43 obese diabetics with type 2 diabetes [23 male and 20 female, BMI=29.82±1.91 kg/m2, aged 42–65 yr], who had previously been treated with different...

ea0014p96 | (1) | ECE2007

High-sensitivity C-reactive protein in diabetes mellitus type II according to micral test findings

Hambali Zarida

Cardiac complications account for three quarter of deaths among diabetic patients. Many studies have shown that high-sensitivity C-reactive protein (hs-CRP) correlated with the inflammatory process of atherosclerosis in the coronary artery. This study is designed to determine the levels of plasma hs-CRP in Type II diabetic patients with microalbuminuria and its association with other biochemical markers used for diabetic monitoring. All biochemical parameters were analyzed usi...

ea0014p97 | (1) | ECE2007

Localization of an ectopic adrenocorticotropin-secreting tumour using 18F- Dopa PET/CT

Dubois Séverine , Morel Olivier , Rodien Patrice , Jeanguillaume Christian , Enon Bernard , Illouz Frédéric , Saint-André Jean-Paul , Girault Sylvie , Rohmer Vincent

Ectopic adrenocorticotropin secretion (EAS) accounts for 10–15% of cases of Cushing’s syndrome and comprises a spectrum of lesions from highly malignant tumours to a variety of less aggressive neuroendocrine tumours. Selective removal of the primary lesion is the optimal management. It is therefore mandatory to localize the source of ectopic ACTH.As no single test is accurate enough to distinguish the ectopic from the pituitary sources of ACTH,...

ea0014p98 | (1) | ECE2007

Adrenocortical carcinosarcoma: first european case report

Somda Frédéric , Leger Julie , Guy Laurent , Norha Olivier , Desbiez Françoise , Boiteux Jean-Paul , Kemeny Jean-Louis , Thieblot Philippe , Tauveron Igor

Adrenocortical sarcoma is an extremly rare tumour associated with a quite pejorative evolution.We report the case of a fifty-eight years old woman presenting a chronic asthenia and acute flank pain. She had no hypertension, no clinical sign of virilization or hypercorticism. Abdominal ultrasonography revealed an 8 centimeters mass above the right kidney. CT scan aspect evoked an adrenal carcinoma embolizing vena cava. Hormonal assays did not reveale any inappropriate secretion...

ea0014p99 | (1) | ECE2007

The genetic association of medullary thyroid carcinoma with Hirschsprung’s disease

Dvorakova Sarka , Vaclavikova Eliska , Skaba Richard , Vlcek Petr , Bendlova Bela

Medullary Thyroid Carcinoma (MTC) can be associated with Hirschsprung’s disease (HSCR). Mutations in exon 10 of the RET proto-oncogene were found in patients with co-occurrence of HSCR and MTC. The aim of the study was to screen the MTC risk exons in patients with HSCR. The genetic analysis comprised 73 HSCR patients (53 males, 20 females) who were operated on and followed-up during 2001-2006. The cohort consisted of 48 patients with classical HSCR, 11 with long colonic a...

ea0014p100 | (1) | ECE2007

Inhibition of C17,20-lyase activity by new 17β-exo-heterocyclic androsterone derivatives

Szécsi Mihály , Tóth István , Wölfling János , Schneider Gyula , Julesz János

17α-Hydroxylase-C17,20-lyase (P45017α) is a key regulator enzyme of the steroid hormone biosynthesis in both the adrenals and the testes. Inhibition of this enzyme can block androgen synthesis in an early step, and may thereby be useful in the treatment of prostatic carcinoma, which is androgen-dependent in the majority of cases. Abiraterone and its analogues have been found strong inhibitors of P45017α suggesting that steroid de...

ea0014p101 | (1) | ECE2007

Cigarette smoking increases high calcitonin levels

d’Herbomez Michele , Bauters Catherine , Leclerc Laurence , Leteurtre Emmanuelle , Carnaille Bruno , Schlienger Jean-Louis , Caron Philippe , Wémeau Jean-Louis

Increased basal or pentagastrin-stimulated calcitonin level is the cornerstone for the biological diagnosis of medullary thyroid carcinoma, but is also observed in patients with C-cell hyperplasia (CCH) of the thyroid. In a prospective multicenter study we re-evaluated the reference ranges of basal calcitonin (bCT) in 287 euthyroid controls without thyroid disease (142 men-45 smokers, 3 deprived, 145 women-27 smokers). The CT levels were measured using 2 different assays (Cis-...

ea0014p102 | (1) | ECE2007

Cortisol excess, inflammatory markers and echocardiographic alterations in adrenal incidentalomas

Ermetici Federica , Malavazos Alexis , Coman Calin , Morricone Lelio , Corsi Massimiliano , Ambrosi Bruno

An increased cardiovascular risk has been described in patients (pts) with adrenal incidentaloma (AI), similarly to pts with overt Cushing’s syndrome (CS). Some echocardiographic abnormalities and alterations in adipokine secretion involved in insulin resistance, inflammation and atherosclerosis have been reported in pts with CS. In this study the possible correlation between echocardiographic parameters and adipokine levels in pts with AI was evaluated.<p class="abst...

ea0014p103 | (1) | ECE2007

Prognostic value of anti-thyroperoxidase antibodies in high malignancy degree breast cancer

Fiore Emilio , Giani Claudio , Giustarini Elisa , Muller Ilaria , Mammoli Claudia , Campani Daniela , Roncella Manuela , Fustaino Loredana , Pinchera Aldo

A high incidence of serum anti thyro-peroxidase antibodies (TPOAb) has been found in breast cancer (BC). Aim of this study was to evaluate the predictive value of TPOAb in BC. The study group included 47 women submitted to mastectomy for high malignancy degree BC (tumor size >5 cm and/or n° lymph-nodes >3), followed for 5 years. No patient had distant metastases. All were evaluated for thyroid disorders after breast surgery and before any anti-tumoral adjuvant the...

ea0014p104 | (1) | ECE2007

Bone density in patients with non-functioning pituitary adenomas (NFA)

Fischli Stefan , Stettler Christoph , Sahli Rahel , Schwab Silvia , Chappuis Bernard , Jenni Stefan , Diem Peter , Christ Emanuel

Background: Surgically treated patients with NFA often present with secondary hypogonadism. Hypogonadism is a well known risk factor for osteopenia or osteoporosis. The aim of this study was to assess (a) the frequency of osteopenia/osteoporosis in a single centre Swiss cohort of patients with operated NFA and (b) whether gender or hypogonadism impacts on bone density at follow up.Methods: Data of patients with NFAs diagnosed between 1967 and 2005 were a...

ea0014p105 | (1) | ECE2007

Echo-enhanced ultrasound has a higher sensitivity than high-resolution CT in the detection of hepatic metastasis of adrenocortical carcinoma

Bauditz Jürgen , Wermke Wolfram , Beyersdorff Dirk , Lochs Herbert , Strasburger Christian J. , Quinkler Marcus

Background: Adrenocortical carcinoma (ACC) is a rare and heterogeneous malignancy with incompletely understood pathogenesis and poor prognosis. Computerized tomography (CT) and magnetic resonance imaging (MRI) are routinely performed for imaging of the adrenal mass and for standard staging of chest and abdomen as lung and liver are the primary organs for metastatic spread in ACC. Contrast ultrasound is a non-invasive procedure which has been shown to have a high sensitivity an...

ea0014p106 | (1) | ECE2007

Characteristics of metabolic syndrome in patients with adrenal incidentaloma

Ivovic Miomira , Vujovic Svetlana , Stojanovic Milos , Tancic-Gajic Milina , Marina Ljiljana , Barac Marija , Drezgic Milka

Several studies show that characteristics of metabolic syndrome are often seen in patients with adrenal incidentaloma. The aim of our study was to evaluate metabolic factors in these patients. 208 patients (148 female and 60 male, age 55.08±11.02 y’s and BMI 27.91±4.6 kg/m2) were admitted and biochemical, endocrine testing were performed. Lipid status: cholesterol 5.77±1.26 mmol/L, triglyceride 1.92±0.98 mmol/L. 113(55%) patients were hypert...

ea0014p107 | (1) | ECE2007

The role of radio-guided surgery (RGS) with the use of 99mTc-EDDA/HYNIC-octreotate in detection of unknown primary and secondary sites of neuroendocrine tumours of the gastrointestinal tract (GEP-NET) and improving the final outcome of patients

Hubalewska-Dydejczyk Alicja , Kulig Jan , Szybinski Piotr , Mikolajczak Renata , Sowa-Staszczak Anna , Fröss-Baron Katarzyna , Huszno Bohdan

Despite a wide spectrum of imaging diagnostics, GEP-NETs often stay undetectable until the time of dissemination. Removing of a primary tumour together with disseminated lymph nodes even with the presence of liver metastases is the most appropriate treatment to delay progression of the disease. SRS followed by RGS gives a possibility to detect occult GEP-NET intra-opratively. 99mTc-HYNIC/EDDA-octreotate, a somatostatin analogue with high affinity to sst2 was applied...

ea0014p108 | (1) | ECE2007

Ascl1 is abundantly expressed in endocrine pancreatic tumors

Johansson Téresè , Halin Lejonklou Margareta , Ekeblad Sara , Stålberg Peter , Skogseid Britt

Background: Apart from inactivation of the MEN1 gene, molecular events essential for tumorigenesis of the endocrine pancreas are poorly characterized. A potentially useful approach for understanding tumor progression is to study transcription factors operating in fetal pancreatic development. The Notch signaling cascade with expression of the transcription factors Hes1, Hey1, and Ascl1 plays a vital role in sustaining the balance between cell prolif...

ea0014p109 | (1) | ECE2007

Histopathological and molecular studies in patients with goiter and hypercalcitoninemia: reactive or neoplastic C-cell hyperplasia?

Verga Uberta , Ferrero Stefano , Vicentini Leonardo , Brambilla Tatiana , Cirello Valentina , Muzza Marina , Beck-Peccoz Paolo , Fugazzola Laura

The cut-off values able to differentiate between reactive or neoplastic C cell hyperplasia (CCH) or to predict sporadic medullary thyroid cancer (MTC) are still debated both for basal and stimulated calcitonin (bCT and sCT). Aim of the present study was to define the prevalence and the histological patterns of CCH in 15 patients with multinodular goiter (MNG), bCT>10 pg/ml and sCT levels >50 pg/ml. These data were compared with those from 16 patients with MNG and bCT l...

ea0014p110 | (1) | ECE2007

Thyroid cancer and pregnancy: clinical outcome and time of diagnosis in a series of 94 women

Michela Perrino , Guia Vannucchi , Leonardo Vicentini , Mannavola Deborah , Beck-Peccoz Paolo , Fugazzola Laura

Thyroid cancer represents the second more frequent tumor among those diagnosed during pregnancy. Indeed, during pregnancy thyroid volume increases by 20–30% and new nodules can appear, due to the effect of choriogonadotropin which stimulates thyroid growth. Hence, it has been proposed that thyroid cancer diagnosed during pregnancy could harbour a poorer prognosis. Aim of the present study was to compare the clinical outcome in the following 3 groups of patients affected w...

ea0014p111 | (1) | ECE2007

RET genotypes comprising specific haplotypes of polymorphic variants are associated with sporadic medullary thyroid cancer

Fugazzola Laura , Muzza Marina , Mian Caterina , Cordella Daniela , Barollo Susi , Cirello Valentina , Elisa Girelli Maria , Opocher Giuseppe , Beck-Peccoz Paolo , Persani Luca

Many single nucleotide polymorphisms (SNPs) of the RET gene have been described both in the general population and in patients with sporadic medullary thyroid cancer (sMTC), MEN2 or Hirschsprung disease. Some association studies reported a higher prevalence of these variants in the affected patients, suggesting a possible role in modifying the risk of occurrence of the disease. However, data from different cohorts of sMTC are discrepant and the aim of the present study ...

ea0014p112 | (1) | ECE2007

Isolation of the Side population (SP) from murine adrenal glands renders cells with adrenocortical stem cell properties

Lichtenauer Urs , Shapiro Igor , Geiger Klaus , Pulichino Anne-Marie , Drouin Jaques , Beuschlein Felix

Radioactive and transgenic tracing experiments indicate that in the adult adrenal stem cells persist in the periphery of the cortex, which migrate centripetally and populate the inner cortical zones upon differentiation. However, investigation of these cells has been hampered by the lack of known marker genes. Vital Hoechst dye exclusion has been described as a method for isolating a side population (SP) from mouse bone marrow, which was enriched with stem cells. Utilizing thi...

ea0014p113 | (1) | ECE2007

Selective intra-arterial calcium stimulation with hepatic venous sampling in investigation of hyperinsulinemic hypoglycemia

Shazli Yusof Mohd , Ahern Tomas , Smith Thomas P , O’Shea Donal

A retrospective analysis of the results of all intra-arterial calcium stimulations performed at St. Vincent’s Hospital, Dublin, in the years 2001-2006. All patients with symptoms suggestive of hypoglycemia had 72 hour fasting test with evidence of inappropriately elevated insulin and c-peptide at the time of hypoglycemia. These patients were investigated further with pancreatic imaging and selective intra-arterial calcium stimulation with hepatic venous sampling (ASVS). A...

ea0014p114 | (1) | ECE2007

Somatostatin and dopamine receptor regulation and effects of a new somatostatin/dopamine chimeric compound on cell proliferation of the human androgen-dependent prostate cancer cell line LNCaP

Arvigo Marica , Ruscica Massimiliano , Dozio Elena , Culler Michael D , Minuto Francesco , Motta Marcella , Magni Paolo , Ferone Diego

The increasing use of somatostatin (SRIF) analogues prompted extensive investigations on SRIF receptor (SSR) in human tumours. Human prostate cancer (PCa) may differentially express SSRs from the normal tissue. Moreover, SSR and dopamine (D) receptors (DR) may interact to form homo- and heterodimers with enhanced functional activity. In the present study, using the human androgen-dependent PCa cell line LNCaP, we investigated: 1) SSR and DR subtype expression in different cult...

ea0014p115 | (1) | ECE2007

Somatostatin receptor regulation and effects of somatostatin and somatostatin analogues on cell proliferation of the human androgen-dependent prostate cancer cell line LNCaP

Ruscica Massimiliano , Arvigo Marica , Dozio Elena , Culler Michael D , Minuto Francesco , Motta Marcella , Ferone Diego , Magni Paolo

Somatostatin (SRIF) has been demonstrated to inhibit in vitro proliferation of normal and transformed cells via SRIF receptors (SSRs). Moreover, like other neuroendocrine molecules, SRIF and SSRs may play a significant role in the progression and neuroendocrine differentiation of human prostate cancer (PCa). However, conflicting results have been reported in the literature on SSR heterogeneity and specific cell localization in PCa. In the present study, using the human ...

ea0014p116 | (1) | ECE2007

Conjugated and unconjugated serum steroid hormone concentrations in relation to tumour receptor status in postmenopausal breast cancer patients

Vincze Borbála , Pommersheim Ferenc , Udvarhelyi Nora , Horváth Zsolt , Kapuvári Bence , Boldizsár Mariann , Köves István , Orosz Zsolt , Láng István

Human breast cancer tissue is able to concentrate estrogens. 17-beta-estradiol (E2) and estrone (E1) are produced locally through several mechanisms, e.g. from conjugated and unconjugated steroid hormones uptaken from the circulation. This study was aimed to investigate the correlation between endogenous serum sex steroid concentrations and tumour receptor status in postmenopausal breast cancer patients undergoing surgical intervention. The study involved 740 postmenopausal pa...

ea0014p117 | (1) | ECE2007

A novel activating germline mutation in the RET gene (Y606C) in a patient with medullary thyroid carcinoma

Luconi Michaela , Ercolino Tonino , Lombardi Adriana , Becherini Lucia , Piscitelli Elisabetta , Sole Gaglianò Maria , Serio Mario , Mannelli Massimo

Germline mutations in the RET gene cause MEN2, an inherited cancer syndrome associated with medullary thyroid carcinoma (MTC). We performed genetic analysis on DNA from whole blood of a 58 yr old female affected by a multifocal MTC. Exons 10, 11, 13, 15 and 16 of RET gene were amplified by PCR using specific primers and characterised by direct automatic sequencing. Here, we report a new RET point mutation: a heterozigous missense mutation Y606C, a G to A nucleotide substitutio...

ea0014p118 | (1) | ECE2007

Novel germline VHL mutations associated to uncommon clinical presentations

Ercolino Tonino , Becherini Lucia , Simi Lisa , Sole Gaglianò Maria , Nesi Gabriella , Valeri Andrea , Mannelli Massimo

The von Hippel-Lindau (VHL) syndrome is an inherited multi-tumor disorder characterized by clinical heterogeneity and high penetrance. Pheochromocytoma (Pheo) is present in 10-15% of cases. It can be isolated or associated with other lesions such as hemangioblastomas, kidney cysts or cancer, and pancreatic lesions. Pheos secrete norepinephrine and are generally located in the adrenals. While performing genetic testing in patients affected by apparently sporadic pheos or PGLs, ...

ea0014p119 | (1) | ECE2007

The expression of alternatively spliced forms of type 1 deiodinase is changed in clear cell renal cell carcinoma

Piekielko-Witkowska Agnieszka , Master Adam , Zbigniew Tanski , Alicja Nauman

Type 1 deiodinase (D1) catalyses deiodination of tyroxine (T4), which leads either to synthesis of triiodothyronine or reverse triiodothyronine (rT3). T3 can influence the process of neoplasia through its receptors which act as transcription factors and regulate the expression of many tumor suppressor genes and oncogenes. The aim of the study was to analyze the changes in expression of alternatively spliced variants of D1 mRNA in clear cell Renal Cell Carcinoma (ccRCC), which ...

ea0014p120 | (1) | ECE2007

Ret expression reduces estrogen-induced lactotrope hyperplasia

Cañibano Carmen , Rodríguez Noela , Tovar Sulay , Vázquez María Jesús , Lavandeira Montserrat , Díeguez Carlos , Álvarez Clara

RET is a tyrosine kinase receptor activated by GDNF, NTN, ART and PSPN through GFRa1,2, 3 and 4 respectively. Activation of the receptor elicits intracellular pathways such us Ras/MAPK and PI3K/AKT leading to differentiation and proliferation. Our group has previously shown that RET is expressed specifically in the somatotroph cell population within the pituitary gland, both in rats and in humans. We have also shown that, in absence of its ligand GDNF, RET induces activation o...

ea0014p121 | (1) | ECE2007

Analysis of BRAF point mutation in papillary thyroid carcinoma

Sykorova Vlasta , Dvorakova Sarka , Laco Jan , Ryska Ales , Kodetova Daniela , Astl Jaromir , Vesely David , Bendlova Bela

BRAF point mutations are found in 29-69% of papillary thyroid carcinoma (PTC). BRAF is a serine-threonine kinase involved in the phoshorylation of MAPK signaling pathway. The mutation is located in the exon 15 of BRAF, resulting in the substitution of valine to glutamate at codon 600 (V600E). Mutation generates unregulated B-Raf activity that leads to increased cellular proliferation. The aim of this study was to determine the frequency of BRAF mutation in the Czech population...

ea0014p122 | (1) | ECE2007

Effect of surgery on carotid vascular remodeling in patients with pheochromocytoma

Bernini Giampaolo , Galetta Fabio , Bardini Michele , Franzoni Ferdinando , Taurino Chiara , Moretti Angelica , Bernini Matteo , Salvetti Antonio

In vitro and in vivo studies suggest that catecholamines, in addition to their hemodynamic effect, exert a direct influence on the vascular wall, leading to eutrophic and hypertrophic remodeling. This finding is in agreement with that recently reported by our group on patients with pheochromocytoma (PHEO) who show carotid intima media thickness (IMT) and vascular fibrosis higher than essential hypertensives matched for classic cardiovascular risk factors, includi...

ea0014p123 | (1) | ECE2007

A case report of ectopic Cushing’s disease presented with trombocytopenia

Ozgil Yetkin Demet , Zuhur Sayid S , Kara Ekrem , Gurel Sayin Ayla , Oz Buge , Tasan Ertugrul

PURPOSE: To report a case of Cushing’s syndome caused by ectopic ACTH secretion related to a thymic carcinoid presented with trombositopenia.CASE: years old male presented with fatigue, skin rash. At presentation, physical findings showed Cushingoid appearance, with moon face, hyperpigmentation, easy bruising and buffalo hump. His laboratory findings showed platelet: 90.000 (150.000–450.000), ACTH: 609 pg/ml (0–46 pg/ml), baseline cortisol...

ea0014p124 | (1) | ECE2007

Influence of Lanreotide Autogel on insulin sensitivity among patients with acromegaly

Eda Ertorer Melek , Bakiner Okan , Anaforoglu Inan , Bozkirli Emre , Bascil Tutuncu Neslihan , Guvener Demirag Nilgun

There are limited data inquiring the relationship between somatostatin analogues and insulin sensitivity among acromegalic cases. This study was conducted to evaluate short-term effects of lanreotide autogel (LA), administered every 28 days by deep subcutaneous injection, on insulin sensitivity among acromegalic patients with pituitary tumors. Before and following six months of LA treatment, insulin resistance and beta-cell function were calculated by using homeostasis model a...

ea0014p125 | (1) | ECE2007

A newly detected mutation of the RET proto-oncogene in exon 8 as a cause of multiple endocrine neoplasia Type 2A

Bethanis S. , Palouka Th. , Avgoustis Ch. , Koutsodontis G. , Bei T. , Yannoukakos D. , Tsagarakis S.

Multiple endocrine neoplasia type2A (MEN 2A) is a syndrome of familial cancers characterized by medullary thyroid carcinoma (MTC), pheochromocytoma and hyperplasia of the parathyroid glands. RET protooncogene is the responsible gene for MEN 2A; in more than 96% of MEN 2A families mutations in RET exon 10 or exon 11 are identified. Herein we report a MEN 2A case affected by a mutation (Gly533Cys) in exon 8. A 66-yr-old male patient was referred to our Department due ...

ea0014p126 | (1) | ECE2007

Clinical and biochemical effects of adjuvant mitotane treatment in patients with adrenocortical cancer (ACC)

Daffara Fulvia , De Francia Silvia , Reimondo Giuseppe , Zaggia Barbara , Sperone Paola , Di Carlo Francesco , Angeli Alberto , Berruti Alfredo , Terzolo Massimo

Objective: Seventeen patients (9 women, 8 men aged 36 years, 22–58) radically resected for ACC were treated with adjuvant mitotane and prospectically followed from 2000 to 2006.Methods: Stage of ACC: was: 1 stage I,; 12 stage II, 4 stage III; Weiss score 6, 3–9; Ki67% 20, 4–67. Eleven patients had functional tumors. Median duration of treatment was 15 months (range:4–84) and 14 patients are currently on mitotane, 2 died, 1 discontinue...

ea0014p127 | (1) | ECE2007

Effectiveness of retinoic acid treatment for redifferentiation of thyroid cancer in relation to recovery of radioiodine uptake

Fernández Fernández Cristián A. , Puig-Domingo Manel , Lomeña Francisco , Estorch Montserrat , Bittini Angel L , Marazuela Mónica , Santamaría Javier , Martín Tomás , Martínez de Icaya Purificación , Moraga Immaculada , Cuesta María , Mejías Ana , Porta Manel , Mauricio Dídac , Halperin Irene

Retinoic acid (RA) treatment has been used in the last decade for redifferentiation of metastatic thyroid cancer that have lost radioiodine uptake (RIup) with heterogeneous results.Aim: To evaluate the improvement of RIup after a course of RA treatment.Method: Retrospective analysis of 29 patients with radioiodine negative metastatic disease (17 men /12 women; 22 papillary, 4 follicular and 3 oncocytic tumours). RA was given at a d...

ea0014p128 | (1) | ECE2007

Expression of the neuropeptide cortistatin in haematological malignancies

Dalm Virgil , Staal Frank , Hofland Leo , van der Lely Aart-Jan , van Hagen Martin

Introduction: Cortistatin (CST) is a 17 amino-acid neuropeptide involved in sleep regulation. Due to its structural resemblance to somatostatin (SS), CST binds with high affinity to the 5 known SS receptors. CST also binds to the putative MrgX2 receptor. Previously we demonstrated that various types of human immune cells and tissues as well as lymphoid cell lines express CST mRNA. We suggested that CST plays a regulatory role in immune cell function both in physiological and p...

ea0014p129 | (1) | ECE2007

A loss-of-function polymorphic mutation in the P2X7 receptor gene in patients with papillary thyroid cancer

Dardano Angela , Falzoni Simonetta , Polini Antonio , Bemi Alessia , Solini Anna , Caraccio Nadia , Di Virgilio Francesco , Monzani Fabio

Extracellular nucleotides, via specific plasma membrane receptors (P2Rs) of the X and Y subtype, modulate several cell functions, including cell-to-cell cross talk. We have previously demonstrated the expression of several functional P2XRs subtypes, including P2X7, in primary human thyrocytes. P2X7 is the main player in inflammation and immunomodulation; a strong expression of this receptor has been shown in several human solid tumors. Polymorphisms of the gene enco...

ea0014p130 | (1) | ECE2007

Enhanced expression of functional P2X7 receptor in human papillary thyroid cancer

Dardano Angela , Ferrari Davide , Cuccato Sabina , Santini Eleonora , Caraccio Nadia , Gulinelli Sara , Callegari Giulia , Faviana Pinuccia , Solini Anna , Di Virgilio Francesco , Monzani Fabio

Extracellular ATP modulates several biological processes via activation of plasma membrane receptors (P2Rs) in normal human thyrocytes (NT). We characterized P2Rs expression and function in two thyroid cancer cell lines: FB1 (anaplastic cancer) and FB2 [papillary cancer (PTC)]. P2Rs expression was evaluated by RT-PCR and WB, intracellular Ca2+changes by fluorimetric technique (Fura2-AM), IL-6 release by ELISA, intracellular [i(ATP)] and extracellular ATP [...

ea0014p131 | (1) | ECE2007

Results 90Y-DOTATATE therapy in patients with neuroendocrine tumours (NETs) - own experience

Kunikowska Jolanta , Hubalewska-Dydejczyk Alicja , Sowa-Staszczak Anna , Krolicki Leszek , Ochman Pawel , Mikolajczak Renata , Pawlak Dariusz , Kobylecka Malgorzata , Maczewska Joanna , Huszno Bohdan

In the 1980 s the discovery of expression of somatostatin receptors on NET cells made the use of somatostatin analogues in diagnosis and therapy possible.The aim: Of the study was to assess response of targeted radio-nuclide therapy with radio-labelled somatostatin analogue 90Y-[DOTA0,D-Phe1,Tyr3]-octreotate (DOTATATE) in treatment of disseminated NETs.Material and methods: 12 patients (a...

ea0014p132 | (1) | ECE2007

Results of treatment of patients with pituitary somatotroph adenomas

Grigoriev Andrey , Molitvoslovova Natalye , Kolesnikova Galina , Kuzmin Anatolij

In ESC for the period since October 2004 till October 2006 were operated 69 patients with acromegaly. Men were 22 (32%), women – 47 (68%). Age of patients changed from 24 till 68 (middle – 47).All patients were separated into 2 groups: surgery (group 1) and combination treatment (group 2), which consist of surgery and somatostatin analogues therapy before and after surgery.In most cases were macroadenomas, only 5 patients...

ea0014p133 | (1) | ECE2007

Adrenal incidentaloma, an oncological or endocrinological enigma? Clinical analysis of 1300 cases observed at a single endocrinological centre

Kasperlik-Zaluska Anna , Roslonowska Elzbieta , Slowinska-Srzednicka Jadwiga , Zgliczynski Wojciech , Jeske Wojciech , Papierska Lucyna , Otto Maciej , Slapa Rafal , Cichocki Andrzej , Cwikla Jaroslaw

Objective: Incidentally found adrenal tumour (adrenal incidentaloma = AI) is the most frequent adrenal disorder. Every patient with AI has to be evaluated carefully to choose the best method of management. We present our experience with a group of 1300 patients with AI, registered at our department.Material and methods: Material consisted of 1300 patients (female/male ratio 2.6, age 10–87 years) with AI ranging in size from 0.8 to 23.0 cm. Methods: ...

ea0014p134 | (1) | ECE2007

Frequency and type of adrenal tumors in our patients

Medic-Stojanoska Milica , Kovacev-Zavisic Branka , Radovanov Tijana , Bajkin Ivana , Novakovic Jovanka , Mitrovic Milena , Tomic Dusan Z , Curic Nilkola , Todorovic-Djilas Ljiljana

In recent years, adrenal tumors (AT) are no rare disease. They may arise from all zones of adrenal cortex and medulla, benign or malignant, sometimes as metastases of distant malignances. Patient’s present hormone excess or mass effect, but part of them is clinically silent. The aim of this study was to investigate the frequency, hormonal secretion and pathohistology of AT in our patients lasting years. All patients with AT which are hospitalized in the period from Januar...

ea0014p135 | (1) | ECE2007

Papillary thyroid cancer – the possible role of death ligands in tumor immunology

Mezosi Emese , Gyory Ferenc , Borbely Agnes , Ujhelyi Bernadett , Soos Gyorgyike , Nagy Endre V. , Varga Zsuzsa , Bajnok Laszlo

Papillary thyroid cancer (PTC) cells and immune cells can kill each other by death ligands. Death ligands induce apoptosis only in sensitive cells. The sensitivity to apoptosis is regulated in a complex and poorly understood manner. The aim of this study was to investigate the Fas ligand (FasL) and Tumor Necrosis Factor-Related Apoptosis Inducing Ligand (TRAIL) expression in PTC cells and tumor infiltrating immune cells. Twenty-six PTCs without and fifteen PTCs with cervical l...

ea0014p136 | (1) | ECE2007

Leptin and adiponectin interact in regulating prostate cancer cell growth

Mistry Tina , Digby Janet , Desai Ken , Randeva Harpal

Introduction: Leptin and adiponectin have opposing properties and are implicated as molecular mediators between obesity and (aggressive) prostate cancer. Adiponectin, circulates inversely proportional to visceral fat accumulation, and has demonstrated anti-proliferative effects in prostate cancer cells; circulating leptin levels increase with visceral fat accumulation and has shown mitogenic effects. We propose that adiponectin and leptin interact in prostate cancer cell growt...

ea0014p137 | (1) | ECE2007

A novel role for Visfatin/Pre-B cell colony-enhancing factor 1 (PBEF)/Nicotinamide phosphoribosyltransferase (NMPRTase) in prostate carcinogenesis

Mistry Tina , Digby Janet , Cross Joe , Desai Ken , Randeva Harpal

Introduction: Visfatin/PBEF is a novel adipokine circulating inversely proportional to visceral fat mass and exerts insulin-mimetic effects; it is expressed in normal, inflamed and tumour tissues. Visfatin/PBEF has also been identified as NMPRTase, a key intracellular enzyme involved in NAD+ metabolism, replenishing NAD+ during cellular respiration. Inhibition of NMPRTase by the anti-cancer agent FK866 has been shown to induce apoptosis in tumours. Prosta...

ea0014p138 | (1) | ECE2007

Initial presentation of patients with acromegaly - analysis of the German acromegaly register

Petersenn Stephan , Reincke Martin , Buchfelder Michael , Franz Holger , Quabbe Hans-Jürgen

Due to its rarity, initial endocrine abnormalities in acromegaly are difficult to investigate in a large cohort, especially with respect to cofounding variables. We searched the German Acromegaly Register for data on the first presentation of patients with acromegaly.Up to November 2005, 1485 patients with acromegaly had been entered into the database. Male patients demonstrated significantly higher random GH (21.0 (0.2–620.0) ng/ml, median (range))...

ea0014p139 | (1) | ECE2007

Survivin – a promising target for immunotherapy in patients with adrenocortical carcinoma

Fassnacht Martin , Wortmann Sebastian , Sbiera Silviu , Kuehner Dorothee , Wobser Marion , Adam Patrick , Becker Juergen C. , Allolio Bruno

Objectives: Adrenocortical carcinoma (ACC) is a rare tumor with poor prognosis and limited therapeutic options. Survivin is an anti-apoptotic molecule expressed by neoplastic and tumor-specific endothelial cells of various carcinomas, but rarely or only weakly in normal differentiated tissue. In melanoma and pancreatic cancer, preliminary results of a survivin vaccination trial ( indicated that an immunological response in patients is often paralleled by...

ea0014p140 | (1) | ECE2007

Thyroid cancer: with an unexpected location – in the pancreas and in an unexpected combination with Boeck’s sarcoidosis

Valkusz Zsuzsanna , Csajbók Éva , Gardi János , Kardos Lilla , Tiszlavicz László , Pávics László

The incidence of differentiated thyroid cancer gradually increased in the last few decades. Primery thyroid cancer is usually located in the thyroid gland and can be classified into well differentiated and poorly differentiated forms. Sooner or later, these cancers metastatize into local lymphnodes or distant organs.We present the histories of two patients with anusual forms of thyroid cancer.A woman of 64 was admitted in our depar...

ea0014p141 | (1) | ECE2007

Thyroglobulin-antibodies in the “normal” range may decrease the diagnostic accuracy of thyroglobulin in the care of patients with differentiated thyroid cancer

Zoltán Locsei , Erzsébet Toldy , István Szabolcs , Gábor Kovács L.

Issue: The use of thyroglobulin (Tg) as tumor marker in differentiated thyroid cancer (DTC) is limited in the presence of thyroglobulin-antibodies (TgAb) but it is generally believed that this is true only for TgAb concentrations over the normal ‘cut off’ point.The aim: Of this study was to investigate if TgAb-s in the normal range, considered to be physiological, may also influence the accuracy and clinical relevance of Tg measurement.<p c...

ea0014p142 | (1) | ECE2007

Dopamine receptor expression and dopamine agonist effectiveness in post-surgical persistent medullary thyroid cancer

Pivonello Rosario , Ferolla Piero , Faggiano Antongiulio , Ferone Diego , Cerasola Enrica , Angeletti Gabriella , Santeusanio Fausto , Minuto Francesco , Lombardi Gaetano , Lamberts Steven WJ , Hofland Leo J , Colao Annamaria

Dopamine receptors was suggested to be expressed in medullary thyroid cancer (MTC). The aim of the current study was to evaluate the expression of D2 dopamine receptor in MTC and the effectiveness of the dopamine agonist cabergoline in patients with MTC. Five paraffin-embedded cases of MTC obtained after thyroidectomy were used to evaluate D2 receptor expression by immunohistochemistry. Fifteen patients (7 males, 8 females, 36–78 years) with post-sur...

ea0014p143 | (1) | ECE2007

Somatostatin analogues and the PI3K-AKT-MTOR-P70S6K pathway: how do they control the proliferation of neuroendocrine tumours?

Franchi Giulia , Grozinsky-Glasberg Simona , de Oliveira Antonio Ribeiro , Salahuddin Nabila , Korbonits Márta , Grossman Ashley B.

Background: Somatostatin analogues are very useful in the treatment of symptomatic neuroendocrine tumours, but effects on proliferation remain unclear. Overexpression of the proto-oncogene protein kinase Akt has been demonstrated in certain endocrine tumours, and activates downstream proteins including mTOR and p70S6K, which play a significant role in cell growth and proliferation. We have therefore explored the site of action of somatostatin in causing inhibition of prolifera...

ea0014p144 | (1) | ECE2007

Angiotensin 4–8 and angiotensin 5–8 inhibit cell proliferation in GH3 rat pituitary lactosomatotroph tumor cell culture

Ptasinska-Wnuk Dorota , Kunert-Radek Jolanta , Lawnicka Hanna , Pawlikowski Marek

Introduction: In many tissues angiotensin peptides acts as the auto/paracrine growth factors. Their effects are dependent on activation of various intracellular signaling pathways, including mitogen-activated protein kinases (MAPK).Angiotensin II (ang II) is the best known angiotensin peptide. The ang II derivatives, angiotensin III (ang III) and angiotensin IV (ang IV) posses biological activity as well. Both ang II and ang IV are known to promote the p...

ea0014p145 | (1) | ECE2007

Epidermal growth factor receptor (EGFR) as a potential new target in the treatment of patients with adrenocortical carcinoma – results of pre-clinical studies

Fassnacht Martin , Hahner Stefanie , Heinrich Barbara , Adam Patrick , Johanssen Sarah , Quinkler Marcus , Koschker Ann-Cathrin , Stroebel Philipp , Marx Alexander , Allolio Bruno

Objectives: Adrenocortical carcinoma (ACC) is a rare malignancy with incompletely understood pathogenesis and poor prognosis. Overexpression of epidermal growth factor receptor (EGFR) has been demonstrated in several tumors and is partly associated with a more aggressive phenotype and a worse prognosis. In addition, targeting the EGFR tyrosine kinase represents a successful new therapeutic strategy, e.g. in non-small cell lung cancer. Therefore, we investigated the role of EGF...

ea0014p146 | (1) | ECE2007

Time necessary to achieve the maximum effect of goserelin, LH-RH agonist, in therapy of hormone dependent breast cancer

Murgic Jure , Matovinovic Martina , Fabijanic Lucija , Katalinic Darko , Vrkljan Milan

Nearly one third of women diagnosed with invasive breast cancer are younger than 50 years with regular menstrual cycles. 60% of these tumors express estrogene and progesterone receptors. Common treatment procedure is surgery followed by chemotherapy, radiotherapy and hormone therapy. Often in younger patients chemotherapy causes permanent amenorrhoea. In case that menses afterwards occurs ovarial suppression is needed, mostly by goserelin, LH-RH agonist. The principle of thera...

ea0014p147 | (1) | ECE2007

Acromegaly due to a lung carcinoid: a case report

malika Rezzoug , Farida Chentli

Acromegaly secondary to a lung carcinoïd is a very rare entity. Secretion of GHRH (Growth hormone releasing hormone) or GHRH like by neuroendocrine tumor induces pituitary hyperplasia and a production of Growth hormone (GH) with or without others anterior pituitary hormones. Total resection of lung tumor induces normalisation of pituitary function as in our observation.AD, 37 years, male, came to our unit for diabetes mellitus and acromegaly. His ch...

ea0014p148 | (1) | ECE2007

Intra- and supra-sellar immature teratoma mimicking pediatric craniopharyngioma

Farida Chentli

Immature teratoma arising from the brain is very rare. The intra and supra sellar localization is very exceptional. Its clinical symptoms and radiological aspects on TDM are similar to those of craniopharyngiomas but on MRI the fat signal characterize teratomas, but only the histological exam gives the confirmation of this last lesion and makes differential diagnosis with mature tumor whose prognosis is better than the immature one. Our observation illustrates all these proble...

ea0014p149 | (1) | ECE2007

Pituitary microprocess

Farida Chentli

Nowadays neuro-radiological explorations are so frequent that radiologists discover more and more pituitary lesions. In this work we would like to study pituitary microlesions (PML: inferior or equal to 10 mm) in order to analyze age and sex repartition, clinical symptoms leading to the diagnosis, position in the pituitary area and the apparent etiologies.All our patients are examined and hormonal exploration is as complete as possible.<p class="abst...

ea0014p150 | (1) | ECE2007

Adrenal incidentalomas and insulin sensitivity – are there any differences between adenomas and hyperplasia?

Dudasova Daniela , Lazurova Ivica , Wagnerova Hedviga , Dravecka Ingrid

It is well known that adrenal masses, particularly adenomas are frequently related to metabolic syndrome and insulin resistance. However, there are no reported data about the differences between adenomas and hyperplasia.Authors examined the prevalence of symptoms of the metabolic syndrome and insulin resistance in 25 patients with adrenal incidentalomas (10 men, 15 women) of the mean age 57.9+15 years. 15 patients had adrenal adenoma determined by CT or ...

ea0014p151 | (1) | ECE2007

Frequency of occurence of MEN1 syndrome in patients admitted with primary hyperthyroidism

Baldys-Waligorska Agata , Sokolowski Grzegorz , Trofimiuk Malgorzata , Golkowski Filip , Huszno Bohdan

Background: Primary hyperparathyroidism (HPT) is the most common endocrinopathy in MEN1 and usually its first clinical manifestation. Yet MEN1 is a rare disease, representing only 2–4% of all cases of HTP. We studied the frequency of MEN1 syndrome in HPT patients admitted to our Department.Methods: In a retrospective analysis of 84 suspected HPT patients hospitalized in 1999–2006, case reports of 11 patients with suspected MEN1 were analysed. M...

ea0014p152 | (1) | ECE2007

Evaluation of the efficacy of sandostatin LAR in the treatment of acromegaly

Baldys-Waligorska Agata , Krzentowska Anna , Golkowski Filip , Huszno Bohdan

Background: Somatostatin analogues are used to treat acromegaly patients who, following surgery, have not fulfilled cure criteria (hGH<2,5 ng/ml, IGF-1 below normal range for age and post-OGTT hGH <1,0 ng/ml). We evaluated the efficacy of Sandostatin LAR in managing such patients.Material and method: In our Clinic, 81 acromegaly patients (mean age 51.6±14.4 yrs) were registered over the years 1983-2005. Based on CT i MRI, macroadenoma and mi...

ea0014p153 | (1) | ECE2007

The beta-HLH transcription factor neurogenin-2 is preferentially expressed by secreting pituitary adenomas

Fratticci Amato , Grieco Fabio , Spilioti Cristina , Giangaspero Felice , Esposito Vincenzo , Santoro Antonio , Ventura Luca , Alesse Edoardo , Jaffrain-Rea Marie-Lise

Beta-HLH transcription factors are involved in the ontogenesis of neural/neuroendocrine cells, and may play a role in the pathogenesis of neuroendocrine tumours. Neurogenin 2 (Ngn2) is expressed by the developing mouse pituitary. After preliminary data indicating its expression in the normal human pituitary, we have studied its phenotypic expression in normal and adenomatous pituitary tissues.Methods: Fifty-two pituitary adenomas (PA) – 23 clinicall...

ea0014p154 | (1) | ECE2007

TGFß1 signalling in human insulinomas compared with human islets.

Nabokikh Anastasiya , Bilban Martin , Gartner Wolfgang , Vila Greisa , Niederle Bruno , Nielsen Jens , Zielinski Christoph , Luger Anton , Wagner Ludwig

Insulinomas are thought to be the result of reduced ß-cell death and hyperproliferation of this specific and highly differentiated cell type. Specific growth- factors are responsible for inducing ß- cell replication and might therefore be involved in insulinoma formation. Pluripotent islet progenitor cells are thought to be located at pancreatic ducts, which can give rise to novel islets as well as exocrine pancreas formation. TGFß1 signalling disruption has bee...

ea0014p155 | (1) | ECE2007

The effect of SOM230 on cell proliferation and cortisol secretion in the human adrenal carcinoma cell line H295R

Mariniello Barbara , Cervato Sara , Opocher Giuseppe , Albiger Nora , Occhi Gianluca , Scaroni Carla , Mantero Franco

Adrenocortical carcinoma (AC) is a rare neoplasm with poor prognosis. Medical treatment of AC is actually based on the use of op’DDD (mitotane) with or without traditional chemotherapeutic agents. Only very few information are available about the effectiveness of somatostatin analogs in AC. In human adrenal gland the expression of all five somatostatin receptor (SSTR) subtypes was previously demonstrated. A differential expression was shown in adrenal adenomas and carcino...

ea0014p156 | (1) | ECE2007

MEN2B – Two simultaneous cases of a rare syndrome

Sallai Ágnes , Hosszú Éva , Gergics Péter , Tulassay Zsolt , Rácz Károly , Fekete György

A 17-year-old boy was referred to our Department. In his medical history Crohn’s disease had been supposed because of abdominal pain and distention. He had previously undergone minor surgery as having large tongue with neuromas and hypertrophic gums. Due to his marfanoid appearance, arachnodactyly, massive eyebrows and lips together with his medical history, multiple endocrine neoplasia type 2B (MEN2B) was suspected, which is a very uncommon hereditary disease. It consist...

ea0014p157 | (1) | ECE2007

Prevalence of autonomous cortisol and aldosterone secretion in patients with a single benign cortical adrenal adenoma after modification of the diagnostic tests

Piaditis George , Kaltsas Gregory , Kounadi Theodora , Markou Athina , Mazarakis Nikos , Gouli Aggeliki , Makras Polyzois , Papadogias Dimitrios , Dimitriou Kostantina , Stathopoulou Alexandra , Kossyvakis Kostantinos , Despoina Ragkou , Kyriakos Vamvakidis , Zografos George , Kontogiorgos George , Chrousos George

Background: The normal cut-offs of screening and diagnostic tests for autonomous aldosterone (AAS) and cortisol (ACS) secretion are poorly defined, mainly due to the presence of adrenal adenomas among those who have served as controls and the stimulating effect of ACTH on aldosterone secretion.Methods: We investigated cortisol and aldosterone secretion in 151 patients with benign cortical adrenal adenomas (BCAA) and in 119 healthy controls with a normal ...

ea0014p158 | (1) | ECE2007

Immunohistochemical detection of angiotensin receptors AT1 and AT2 in adrenal tumors

Pawlikowski Marek , led Beata , Winczyk Katarzyna

Immunohistochemical detection of angiotensin receptors AT1 and AT2 in adrenal tumorsAngiotensin II is well known to affect the adrenal cell growth and function. Angiotensin receptors AT1 and AT2 were found to be present in the normal adrenal gland. However, the data on the expression of angiotensin receptors in the adrenal tumors is very scarce.To overcome this gap, the paraffin sections of the adrenal cortical tumors and of pheoch...

ea0014p159 | (1) | ECE2007

Bilateral adrenal incidentalomas: exploration of aberrant responses and comparison with unilateral lesions

Vicha Eirini , Vassiliadi Dimitra-Argyro , Avgoustis Christos , Palouka Theodossia , Tsagarakis Stylianos

Aberrant hormone receptors have been demonstrated in macronodular adrenal hyperplasia or, rarely, unilateral adenomas causing Cushing’s syndrome but their prevalence in adrenal incidentalomas (AI) remains uncertain. Therefore we evaluated patients with bilateral AI for evidence of abnormal response to physiological stimuli. We also compared their biochemical characteristics with those of patients with unilateral AI.Assessment of adrenal function was...

ea0014p160 | (1) | ECE2007

Inhibitory effect of rosiglitazone – PPARgamma receptor ligand on growth of human adrenocortical tumor cells in vitro

Winczyk Katarzyna , Lawnicka Hanna , Fuss-Chmielewska Julita , Kolomecki Krzysztof , Kuroszczyk Jacek , Pawlikowski Marek

Introduction: The peroxisome proliferator-activated receptors gamma (PPARγ) are nuclear receptors which are detected in normal and pathological tissues. Our earlier study showed the overexpression of PPARγ in human adrenal tumors and pituitary adenomas in comparison to normal glands. The in vitro experiments indicated that ligands of PPARγ inhibit growth of many tumors including pituitary adenomas, thyroid cancers and adrenal carcinomas. However, the data...

ea0014p161 | (1) | ECE2007

The modern pre- and intraoperative diagnostic algorithm of pancreatic NET with the use of 99mTc-EDDA/HYNIC-octreate scintigraphy – the impact of SRS on patients’ management

Hubalewska-Dydejczyk Alicja , Fröss-Baron Katarzyna , Mikolajczak Renata , Huszno Bogdan , Szybinski Piotr , Kulig Jan , Januszewska Aleksandra , Sowa-Staszczak Anna , Pach Dorota

Pancreatic NETs often cause difficulties in imaging diagnostics and optimal diagnostic algorithm is searched for. According to the latest reports MDCT sensitivity amounts 60-90%, MR: 80-90%, SRS: 62-100%, EUS: 70-90%.Aim: Assessment of the usefulness of 99 mTc-EDDA/HYNIC-octreotae scintigraphy in detection of primary and metastatic tumours of pancreatic NET in comparison to CT, EUS and IOUS and evaluation of the impact of scintigraphic results...

ea0014p162 | (1) | ECE2007

Segregation of P25L and S80I mutations of the vhl gene in an extended Hungarian family with von Hippel-Lindau syndrome

Patócs Attila , Balogh Katalin , Tóth Miklós , Fazakas Ferenc , Likó István , Rácz Károly

Background: von Hippel-Lindau syndrome (VHL) is a rare autosomal dominant disease caused by alterations of the vhl tumor-suppressor gene. Patients with VHL are at risk for development of retinal, central nervous system and spine hemangioblastomas, clear-cell renal cell carcinomas, pheochromocytomas, endolymphatic sac tumors and cysts; and pancreatic islet cell tumors. Based on the presence or absence of pheochromocytoma as a phenotypic marker, VHL can be divided into di...

ea0014p163 | (1) | ECE2007

High prevalence of novel mutations of the MEN1 gene in Hungarian patients with multiple endocrine neoplasia type 1

Balogh Katalin , Hunyady László , Patócs Attila , Gergics Peter , Valkusz Zsuzsa , Tóth Miklós , Varga Ibolya , Gláz Edit , Rácz Károly

Introduction: Multiple endocrine neoplasia type 1 (MEN 1) may present as a familial or a sporadic disorder with multiple endocrine tumours including parathyroid adenomas or hyperplasias, tumours of endocrine pancreatic and pituitary gland. Familial and sporadic MEN 1-related states which do not fulfill current diagnostic criteria but may be related to MEN 1 syndrome have been also described.Aims: The aim of this study was to examine the prevalence and sp...

ea0014p164 | (1) | ECE2007

Analysis of germline mutations in patients with pheochromocytomas and paragangliomas

Krawczyk Aleksandra , Hasse-Lazar Kornelia , Ziaja Jacek , Pawlaczek Agnieszka , Krajewska Jolanta , Peczkowska Mariola , Preibisz Aleksander , Kubaszek Agata , Januszewicz Andrzej , Jarzab Barbara

There are two types of neoplasms derived from chromaffine tissue: pheochromocytomas (tumors of adrenal core) and paragangliomas (tumors located extraadrenally). Majority of these tumors are sporadic, although according to literature, when DNA analysis is carried out, hereditary disease can be diagnosed in about 25% of patients: Multiple Endocrine Neoplasia type 2 (MEN2A and MEN2B), von Hippel-Lindau Syndrome (VHL), Pheochromocytoma/Paraganglioma Syndrome (PPS) and neurofibroma...

ea0014p165 | (1) | ECE2007

RET exon 13 germline polymorphism in patients with pheochromocytomas and paragangliomas

Hasse-Lazar Kornelia , Pawlaczek Agnieszka , Krawczyk Aleksandra , Wiench Malgorzata , Oczko-Wojciechowska Malgorzata , Peczkowska Mariola , Preibisz Aleksander , Kubaszek Agata , Januszewicz Andrzej , Ziaja Jacek , Jarzab Barbara

Germinal mutations in protooncogene RET are associated with the inherited medullary thyroid carcinoma (MTC) which occurs as the sole manifestation of disease (FMTC) or, more frequently, as the part of multiple endocrine neoplasia (MEN2). The contribution of RET polymorphism to the occurrence of apparent sporadic MTCs is controversial. In our previous study we have found out that the frequency of RET 769 CTT>CTG polymorphism in patients with MTCs is not significantly higher...

ea0014p166 | (1) | ECE2007

Cabergoline suppression test in distinguishing the variability of response to dopamine agonists in prolactinomas

BADIU Corin , VOICU Daniela , Caragheorgheopol Andra , Hortopan Dan , Silvestro Luigi

Primary therapy in prolactinomas, the most frequent pituitary adenomas, consists in ergot derivatives dopamine agonists (bromocriptine or cabergoline) which lowers prolactin levels and shrink the tumour. Bromocriptine was the first drug used, but the therapeutic levels are attained after several days/weeks, therefore an acute suppression test is not possible. However, the biological response is variable and 10% of prolactinomas are resistant to medical therapy. In order to eva...

ea0014p167 | (1) | ECE2007

Predictive value of pituitary histology on clinical outcome in acromegaly: a retrospective cohort study

Steffin Birgit , Saeger Wolfgang , Quabbe Hans-Jürgen , Petersenn Stephan , Ludecke Dieter K , Honegger Jürgen , Buchfelder Michael , Reincke Martin

Immunohistochemistry is commonly performed on tumour specimen obtained during transsphenoidal surgery, but its predictive value for clinical outcome is largely unknown. The aim of this study was to compare clinical and biochemical outcome characteristics after surgery with histological tumour properties. This was achieved by matching data from the German Acromegaly Register with those of the Pituitary Tumour Registry of the German pituitary working group. From 285 out of 1543 ...

ea0014p168 | (1) | ECE2007

Somatostatin receptor immunohistochemistry in neuroendocrine tumors: a proposal of scoring system for clinical characterization and therapy selection

Faggiano Antongiulio , Volante Marco , Pia Brizzi Maria , La Rosa Stefano , Rapa Ida , Ferrero Anna , De Chiara Anna , Righi Luisella , Capella Carlo , De Rosa Gaetano , Dogliotti Luigi , Colao Annamaria , Papotti Mauro

Background: Typing somatostatin receptor (SSTR) expression in neuroendocrine tumors (NETs) is of relevance to target an octreotide-based diagnostic approach and treatment. The expanding use of immunohistochemistry to detect SSTR is to date not paralleled by an accurate methodological setting and standardized interpretation of the results.Objective: A multicentric study was designed to compare SSTR immunohistochemical expression with in vivo scinti...

ea0014p169 | (1) | ECE2007

Prevalence of primary aldosteronism among hypertensive patients (preliminary results)

Matrozova Joanna , Zacharieva Sabina , Kirilov Georgi , Boyanov Mihail , Hristov Vladimir

Until recently primary aldosteronism /PA/ was thought to be rare, accounting for no more than 0.05–2% of the hypertensive patients. Studies published in the last decade demonstrate that primary hyperaldosteronism is a much more common cause of secondary hypertension than was previously thought, accounting for as many as 5% to 25% of hypertensives in some series. For the present, there are no data concerning the prevalence of PA in Bulgaria which determined the realization...

ea0014p170 | (1) | ECE2007

Leptin modulates the growth of murine Colon 38 cancer and interferes with the cytotoxic effect of fluorouracil in vitro

Melen-Mucha Gabriela , Lawnicka Hanna

Epidemiological studies underline that obesity represents a significant risk factor for development of several cancer among them colon cancer. Moreover, multiple recent data indicate that some of adipose tissue-derived hormones may influence the growth of malignant cells. Leptin, the product of the ob gene, is one of them. However, research is still contradictory regarding the role of leptin in colon cancer.The aim of our study was to examine the ...

ea0014p171 | (1) | ECE2007

Muscle mitochondrial function is impaired in patients with prior acromegaly

Szendrödi Julia , Zwettler Elisabeth , Schmid Albrecht Ingo , Chmelik Marek , Pacini Giovanni , Kacerovsky Gertrud , Schnack Christoph , Schernthaner Guntram , Wagner Oswald , Klaushofer Klaus , Roden Michael

Acromegaly is amongst other symptoms associated with myopathy, alterations of energy expenditure and insulin action which are mediated by growth hormone (GH) and insulin-like growth factors (IGFs). It is unclear to which extent these abnormalities remain after treatment. Thus, we examined glucose metabolism, intracellular fat deposition and mitochondrial function in patients with prior acromegaly (AM).Six AM (4f/2 m, age: 49±10 years, body mass inde...

ea0014p172 | (1) | ECE2007

Diagnosis and treatment of the ACTH-secreting neuroendocrine pancreatic tumors

Aleksandra Gilis-Januszewska , Alicja Hubalewska-Dydejczyk , Malgorzata Trofimiuk , Dorota Pach , Magdalena Szurkowska , Monika Buziak-Bereza , Elwira Mazurek , Bohdan Huszno

Introduction: Neuroendocrine tumors secreting ACTH are a rare cause of Cushing’s syndrome.Diagnostic and therapeutical difficiulties might be caused due to different clinical picture of of neuroendocrine tumors.Patients, diagnostic and therapeutic approach: During 2004–2005 2 female patients 32-years old AL and 67-years old ZS were hospitalized in Endocrinology Department due to severe hypercorticism signs and symptoms. I...

ea0014p173 | (1) | ECE2007

Novel mutations in genes encoding succinate dehydrogenase complex subunits B (SDHB) and von Hippel-Lindau protein (VHL) in patients with nonsyndromic pheochromocytoma

Vignjevic Jovana , Nikolic Jelena , Isailovic Tatjana , Mirkovic Katarina , Petakov Milan , Antic Jadranka , Macut Djuro , Beleslin-Cokic Bojana , Popovic Bojana , Bozic Ivana , Ognjanovic Sanja , Damjanovic Svetozar

Background: Several susceptibility genes have been found to be associated with development of pheochromocytoma (PHEO): RET, VHL, SDHB and SDHD. We investigate the frequency of germ-line mutations in SDHB and VHL genes in patients with apparently sporadic PHEO.Material and methods: Fifty patients (38 women, mean age 42) with apparently sporadic adrenal and extra-adrenal PHEO were screened. DNA was extracted from whole blood and from paraffin embedded tumo...

ea0014p174 | (1) | ECE2007

Evaluation of plasma and urinary metanephrines as well as serum chromogranin A for the diagnosis of pheochromocytoma

Unger Nicole , Pitt Christian , Walz Martin K , Mann Klaus , Petersenn Stephan

Adrenal pheochromocytomas are neoplasms characterized by catecholamine excess. We recently reported on the diagnostic value of plasma metanephrines measured by RIA for the diagnosis of pheochromocytoma. However, RIA may not be used in many laboratories.This study evaluated plasma and urinary metanephrines determined by a newly available ELISA as well as serum chromogranin A (CgA) for the diagnosis of pheochromocytoma. Spontaneous blood samples and 24h-ur...

ea0014p175 | (1) | ECE2007

11C-5-hydroxytryptophan PET scan in diagnosis of ectopic Cushing’s syndrome from typical lung carcinoid: a case report

Macut Djuro , Damjanovic Svetozar , Bjekic Jelica , Popovic Bojana , Bozic Ivana , Petakov Milan , Ognjanovic Sanja , Isailovic Tanja , Joksomovic Miloje , Jakovic Radoslav , Mirkovic Jelena , Stojsic Jelena

A 34-year-old woman was initially presented with clinical signs of Cushing’s syndrome (CS). On endocrinological examination, a diagnosis of ACTH dependent CS was established (serum cortisol: 08.00 h: 1245 nmol/l; 24.00 h: 275; plasma ACTH concentration 104 ng/l; inadequate cortisol suppresion on LDDST (787) and suppresion to 318 following HDDST). A magnetic resonance imaging (MRI) confirmed a microadenoma in the left part of the pituitary. Ultrasound examination confirmed...

ea0014p176 | (1) | ECE2007

Mutational analysis in patients with nonsyndromic MEN1

Mirkovic Katarina , Beleslin-Cokic Bojana , Vignjevic Jovana , Nikolic Jelena , Antic Jadranka , Popovic Bojana , Isailovic Tatjana , Bozic Ivana , Petakov Milan , Macut Djuro , Ognjanovic Sanja , Damjanovic Svetozar

Background: Mutational screening of the MEN1 gene has been recommended for patients who fulfill clinical criteria for familial or sporadic MEN1 and those suspicious or atypical of MEN1.Patients and methods: Eighteen apparently unrelated individuals (6 males; 12 females, age range 16–71) with clinical manifestations of MEN1 were analised. In addition, we evaluated 7 relatives. Genomic DNA from peripheral blood leucocytes was extracted using st...

ea0014p177 | (1) | ECE2007

Screening for mutations in exon 10, 11, 13 and 14 of the RET protooncogene associated with inherited medullary thiroid carcinoma (MTC) in Serbian population

Nikolic Jelena , Vignjevic Jovana , Mirkovic Katarina , Beleslin-Cokic Bojana , Antic Jadranka , Isailovic Tatjana , Petakov Milan , Damjanovic Svetozar

Background: Ret protooncogene germ-line mutations are associated with the inherited multiple endocrine neoplasia type 2 syndromes (MEN2a and MEN2b) and also with familial medullary thyroid carcinoma (FMTC). In this study, we report a large scale of mutations in exon 10, 11, 13 and 14 RET protoocogene in patients from Serbia. Our study included patients with MTC.Methods: Our study included 180 patients. Patients were tested for RET protooncogene mutations...

ea0014p178 | (1) | ECE2007

The use of 18F-FDG PET/CT with or without rhTSH stimulation during follow-up of patients with differentiated thyroid carcinoma

Lupoli Gelsy Arianna , Panico Annalisa , Colarusso Sara , Fonderico Francesco , Gonnella Antonio , Poggiano Maria Rita , Pucci Luigi , Martinelli Addolorata , Nicolai Emanuele , Salvatore Marco , Lupoli Giovanni

Background: Fluorine-18 fluorodeoxyglucose positron emission tomography (FDG-PET) is a new method employed in the management of differentiated thyroid cancer (DTC). The integrated FDG-PET plus computed tomography (PET/CT) fusion imaging system seems able to provide some additional advantages over PET alone, mainly related to a better anatomical localisation of the hypermetabolic metastatic lesions. The influence of serum TSH levels on 18F-FDG uptake by recurrences o...

ea0014p180 | (1) | ECE2007

Lower catch-up growth under rGH therapy at pre-pubertal pituitary dwarves diagnosed at an older age

Branisteanu Dumitru , Galesanu Corina , Cristea Cristina , Zbranca Eusebie , Vulpoi Carmen , Preda Cristina , Mogos Voichita

Growth hormone deficiency leads to profoundly decreased growth velocity and, when untreated, to pituitary dwarfism. We evaluated growth evolution for one to four years under rGH therapy (0.07 IU/kg/day, subcutaneously) at seventeen idiopathic pituitary dwarves with isolated GH deficiency, 13 boys and 4 girls, with a wide span of age at therapy onset (between 4 and 24 years old). Diagnosis was set subsequent to at least two negative GH stimulation tests. All patients were pre-p...

ea0014p181 | (1) | ECE2007

The growth hormone – insulin-like growth factor-I axis in adult thalassemic patients

Danesi Leila , Cattaneo Agnese , Valassi Elena , Scacchi Massimo , D’Angelo Emanuela , Mirra Nadia , Zanaboni Laura , Cappellini Maria Domenica , Cavagnini Francesco

GH deficiency (GHD) can be recognized in a not negligible proportion of thalassemic children, while data on the prevalence of this disorder in adult patients are lacking. Therefore, we elected to study the GH – IGF-I axis in a large group of adult thalassemic subjects.Study design: Ninety-four patients (69 with thalassemia major and 25 with thalassemia intermedia on stable transfusional regimen, 39 men and 55 women, aged 31.5±6.8 years, receivi...

ea0014p182 | (1) | ECE2007

The role of BMP-3B in the establishment of zona glomerulosa in the adrenal gland

Bakmanidis Artem , King Peter

The adrenal gland is composed of the medulla and the cortex, which is further subdivided into three zones: zona glomerulosa (zG), zona fasciculata (zF) and zona reticularis (zR). The zones of the cortex are functionally characterised by their ability to synthesise different steroids and consequently they express different steroidogenic enzymes. These and other markers of the zones have been described but so far no good candidate for a determining factor of zonal establishment ...

ea0014p183 | (1) | ECE2007

Selenium supply modulates growth spurt of selenoprotein P knockout mice

Renko Kostja , Michaelis Marten , Schweizer Ulrich , Köhrle Josef , Schomburg Lutz

Background: Selenoproteins are playing an important role in body homeostasis and development. They control thyroid hormone metabolism and are of prime importance for intracellular redox regulation and cellular defence. The SePP knockout mouse (SePP-KO) is a model of impaired Se metabolism characterized by a disrupted distribution system for organified Se. One of the major phenotypes of the homozygote SePP-KO mice is a reduced increase in size and weight during the growth spurt...

ea0014p184 | (1) | ECE2007

Factors affecting height velocity (HV) during GnRH analog therapy in girls with central precocious puberty (CPP)

Thomas Dimitrios , Vlachopapadopoulou Elpis , Karachaliou Feneli , Vourliotaki Eirini , Kaldrimides Philippos , Michalakos Stefanos

Aim: To assess factors affecting HV during triptorelin suppression treatment for CPP.Materials and methods: Forty-six girls with CPP, with mean age at diagnosis 8.4 yrs who presented with signs and symptoms of puberty before the age of 8 years and were treated with triptorelin for at least 2 years, were studied. All girls were categorized into three groups according to the difference between bone age (BA) and chronological age (ΔBA-CA): group I with...

ea0014p185 | (1) | ECE2007

Auxological and IGF system parameters in African in comparison with western countries normal children

Boschetti Mara , Piredda Sabrina , Sidoti Marilena , Di Battista Eliana , Larizza Daniela , Ferone Diego , Barreca Antonina , Minuto Francesco

Growth is an integrated process, resulting from the response of cells to nutrient availability and to hormonal status. Nutrients, in turn, are important regulators of IGF-IGFBP system which are critical regulators of growth. Genetic factors seem to be very important determinant of final stature in countries with high quality of life at variance with underprivileged countries where food intake deficiency is critical. The aim of our study was to evaluate the influence of environ...

ea0014p186 | (1) | ECE2007

X-linked neuronal T3 transport defect: Allan Herndon Dudley syndrome

Dugal Tabinda , Albanese Assunta , Mc Entagart Muriel

Thyroid hormone is absolutely necessary for early brain development.Incidence of thyroid disorders in infancy is 1:4,000.Thyroid hormones can be deficient through hormone synthesis and action or very rarely through defective transport. Some new and exciting transporters for tri-iodothyronine (T3) have recently come to light.MCT 8 gene encodes the protein that transports T3 into neurons. Its mutation result in inability of T3 to enter a developi...

ea0014p187 | (1) | ECE2007

Cephalometric analysis and dental maturation in patients with Turner’s syndrome (TS)

Gomes Rafaella Shimada , Oliveira Darwin Luiz Martins , Ramos-Dias João Carlos , Nigri Alcinda Aranha , Gianini Reinaldo José , Nonoyama Sérgio Yassuo , Senger Maria Helena

Craniofacial proportions of girls with TS, compared to normal children, show reduced size of the craniofacial complex, retrognathic profile and tendency towards advanced dental age. Growth hormone (GH) treatment in TS positively affects stature, but its effects on craniofacial growth and dental development are largely unknown. The aim of this study was to analyze and to correlate the craniofacial morphology, chronological, dental and bone ages of TS patients receiving GH or no...

ea0014p188 | (1) | ECE2007

Craniofacial development and dental maturation in growth hormone(GH)-deficient patients

Oliveira Darwin Luiz Martins , Gomes Rafaella Shimada , Senger Maria Helena , Nigri Alcinda Aranha , Gianini Reinaldo José , Nonoyama Sérgio Yassuo , Ramos-Dias João Carlos

Growth is a complex process, influenced to a large extent by GH. Children with GH deficiency (GHD) have typical somatic features, including short stature and a reduction of the craniofacial structures. Dental age (DA) is normally delayed in relation to chronological age (CA). The effect of GH replacement on craniofacial growth is still poorly understood. We studied the craniofacial development and dental maturation in 17 patients (4F, 13M) with GHD of different etiologies. The...

ea0014p189 | (1) | ECE2007

Developing brain as an endocrine gland secreting GnRH and dopamine to general circulation

Ugrumov Michael

This study was aimed to test our hypothesis that the brain-derived gonadotropin-releasing hormone (GnRH) and dopamine (DA) are delivered to the general circulation in fetal and neonatal rats, i.e. before the establishment of the blood-brain barrier, that is in contrast to adult rats. The GnRH and DA concentrations were measured in plasma and in the brain on the 18th embryonic day (E18), E21, 3rd postnatal day (P3), i.e. before the establishment of the blood-brain barrier, and ...

ea0014p190 | (1) | ECE2007

Closure by iptakalim, a cardiovascular K(ATP) channel opener, of rat islet beta-cell K(ATP) channels and its molecular basis

Suga Sechiko , Wakui Makoto , Kudo Takanori , Ogawa Yoshiji

Diabetes mellitus is a group of diseases characterized by high levels of blood glucose resulting from defects in insulin production, insulin action, or both. Diabetes patients usually have accompanying cardiovascular disorders. Sulfonylureas have been the leading oral antihyperglycemic agents for type 2 diabetes treatment, which currently still constitute the most popular anti-diabetic drugs. Nevertheless, concern has arisen over the side effects of sulfonylureas on the cardio...

ea0014p191 | (1) | ECE2007

Plasma adiponectin and leptin levels in obese and insulin resistant postmenopausal females with type 2 diabetes

Adamia Nona , Tsotsonava Tea , Janberidze Ketevan

Objective: Adipocytokines appear to be important in regulating insulin sensitivity. The objective of this study was to compare the levels of adiponectin and leptin in lean, obese and obese diabetic (OD) postmenopausal female (PMF) subjects during 6 months follow-up of Metformin therapy (MT).Methods: We examined plasma levels of adiponectin and leptin in 26 OD PMF with a mean body mass index (BMI) of 36.6±1.8, 10 obese (BMI=35.9±2.2) and 10 lean...

ea0014p192 | (1) | ECE2007

Pioglitazone modifies the effects of growth hormone on lipolysis and insulin sensitivity

Krag Morten B , Nielsen Søren , Guo ZengKui , Pedersen Steen B , Schmitz Ole , Christiansen Jens S , Jørgensen Jens OL

Context: Thiazolidiones (TZD) improves insulin sensitivity in type 2 diabetes via effects on fat metabolism, whereas growth hormone (GH) stimulates lipolysis and induces insulin resistance.Objective: To evaluate the effects of TZD on fat metabolism and insulin sensitivity in GH-treated GH deficient (GHD) patients.Design: Randomized, placebo-controlled, double-blind parallel-group study including 20 GHD patients on continued GH repl...

ea0014p193 | (1) | ECE2007

The metabolic syndrome and associated sexual dysfunction: psychobiological correlates

Corona Giovanni , Mannucci Edoardo , Petrone Luisa , Mansani Riccardo , Balercia Giancarlo , Chairini Valerio , Forti Gianni , Maggi Mario

Objectives: The aim of present study is to determine psychobiological characteristics of sexual dysfunction (SD) associated with metabolic syndrome (MS; as defined by National Cholesterol Education Program’s Adult Treatment Panel III, NCEP-ATP-III criteria) in a consecutive series of 803 male out-patients.Methods: Several hormonal, biochemical and instrumental (penile doppler ultrasound, PDU) parameters were studied, along with psycho...

ea0014p194 | (1) | ECE2007

A comparison of NCEP-ATP-III and IDF metabolic syndrome definitions with relation to metabolic syndrome associated sexual dysfunction

Corona Giovanni , Mannucci Edoardo , Petrone Luisa , Lotti Francesco , Fisher Alessandra D , Balercia Giancarlo , Chairini Valerio , Forti Gianni , Maggi Mario

Objectives: The aim of present study was to verify possible differences in the prevalence of vasculogenic ED and hypogonadism comparing two distinct new definitions of MetS, as National Cholesterol Education Program-Third Adult Treatment Panel (NCEP-ATPIII) and International diabetes Federation (IDF) in patients with sexual dysfunction.Methods: Several hormonal, biochemical and instrumental (penile doppler ultrasound) parameters were studied. ANDROTEST S...

ea0014p195 | (1) | ECE2007

Effect of supervised structured exercise program for 16 weeks on metabolic, pulmonary and cardiovascular parameters in obese adolescents

Guízar-Mendoza Juan , Linares Benigno , Amador Norma , Barbosa Gloria , Malacara Juan

Objective: To investigate whether a supervised structured exercise program by 16 weeks improves metabolic, pulmonary and cardiovascular parameters in obese adolescents.Material and methods: We included 38 obese adolescents between 12–15 years old. They participated in a supervised exercise program by 90 minutes, 5 days a week during 16 weeks. At baseline and at the end of the exercise program, we evaluated cardiorespiratory fitness, anthropometric m...

ea0014p196 | (1) | ECE2007

Continuous administration of dihydrotestosterone or letrozole to immature female rats results in polycystic ovary syndrome characteristics at adult age

Mannerås Louise , Lystig Theodore , Holmäng Agneta , Ottosson-Lönn Malin , Stener-Victorin Elisabet

Introduction: Polycystic ovary syndrome (PCOS) is a complex endocrine and metabolic disorder associated with ovulatory dysfunction, hyperandrogenism, abdominal obesity and insulin resistance. Since hyperandrogenism is a PCOS key feature, the aim was to evaluate the effects of androgen receptor activation in terms of continuously administration, beginning pre-pubertal, of either the non-aromatizable androgen dihydrotestosterone (DHT) or the aromatase inhibitor letrozole (L), on...

ea0014p197 | (1) | ECE2007

Neonatal sex steroid exposure of female rats results in insulin resistance and enlarged mesenteric adipocytes

Alexanderson Camilla , Lystig Theodore , Stener-Victorin Elisabeth , Lönn Malin , Holmäng Agneta

Introduction: Neonatal events may contribute to the development of disorders such as type 2 diabetes and obesity at adult age. We have previously shown that neonatal testosterone (T) programming of female rats is followed by insulin resistance and changes in adipose tissue distribution with centralization of body fat. Therefore, the aim of this study was to examine the effects of neonatal injection of T, estradiol (E) or dihydrotestosterone (DHT) on insulin sensitivity and siz...

ea0014p198 | (1) | ECE2007

Evaluation of visceral protein malnutrition in morbid obese patients operated on laparoscopic gastric bypass

Casañ Rosa , Morillas Carlos , Sola Eva , García Katherinne , Yanini Jesus , Jover Ana , Gomez Marcelino , Hernández Antonio

Morbid obesity is associated with a decreased life expectancy and a myriad of serious medical problems. The Roux-en-Y gastric bypass (RYGBP) is the most effective procedure for the treatment of these patients, but it can be responsible of early and late complications. The aim of this prospective study was to evaluate the rate of visceral protein malnutrition (VPM) in morbid obese (MO) patients two years after laparoscopic RYGBP. Albumin (Alb), prealbumin (Prealb), transferrin ...

ea0014p199 | (1) | ECE2007

Overconsumption of salty and sweet foods increases blood pressure in children

Petrou Vassilios , Tertipi Athanasia , Papathanasiou Asteroula , Zerva Ourania , Skarpa Vassiliki , Papachilleos Petros , Anastasacou Marina , Nicolopoulos Panagiotis , Hadjiathanasiou Charalambos

Aim: To estimate the impact of overconsumption of salty and sweet foods on Body Mass Index (BMI) and Blood Pressure (BP) in children.Patients-Methods: We studied 208 children (105 girls), aged 9.2±3.0 yr, 57 (27.4%) of normal weight, 37 (17.8%) overweight and 114 (54.8%) obese. Overconsumption of NaCl was considered >5 g/day and of free sugar >0.5 g/Kg ideal Body Weight/day. BP was measured as appropriate and BMI was estimated in all childre...

ea0014p200 | (1) | ECE2007

Expression of PKA regulatory subunits inversely correlates with BMI and insulin resistance parameters in human adipocytes from lean and obese subjects

Bondioni Sara , Mantovani Giovanna , Alberti Luisella , Corbetta Sabrina , Gilardini Luisa , Invitti Cecilia , Zappa Marco Antonio , Ferrero Stefano , Peverelli Erika , Lania Andrea G , Beck-Peccoz Paolo , Spada Anna

In human adipocytes the cAMP-dependent pathway mediates signals originating from the activation of beta-adrenergic receptors, thus playing a key role in the regulation of important metabolic processes such as lipolysis and thermogenesis. CyclicAMP effects are mainly mediated by cAMP-dependent protein kinase (PKA), a tetrameric enzyme composed by two catalytic subunits associated with two regulatory (R) subunits. There are four different R subunit genes and proteins (R1A, R1B, ...

ea0014p201 | (1) | ECE2007

Insulin resistance and fasting leptin’s relationship in subjects with metabolic syndrome

Mokhort T , Shishko E

Background and aims: The aim of our study is to investigate the possible associations between leptin and fasting insulin and index HOMA-IR in patients with Metabolic Syndrome as Leptin is involved in regulation of body weight.Materials and methods: The study included 100 patients (32 m, 68 f) 25–65 years. They were divided into 2 groups matched by sex, age, weight, body mass index (BMI), waist-to-hip ratio (WHR). Research group included 56 patients ...

ea0014p202 | (1) | ECE2007

Epicardial adipose tissue, hepatic steatosis and obesity

Cikim Ayse Sertkaya , Topal Ergun , Harputluoglu Murat , Keskin Lezzan , Zengin Zeynep , Cikim Kerim , Ozdemir Ramazan , Aladag Murat , Yologlu Saim

Objective: The measurement of epicardial adipose tissue (EAT) sonographically is reported to be related with both obesity and insulin resistance. Hepatic steatosis is one of the best known other coincidence with obesity. We aimed to evaluate the relationships between EAT thickness, hepatic steatosis and insulin resistance in obese patients.Methods: Obese 63 subjects were enrolled into the study. Local ethical committee approval was obtained. Patients wer...

ea0014p203 | (1) | ECE2007

Decreased 11beta-hydroxysteroid dehydrogenase type 1 activity in obese boys

Wiegand Susanna , Richardt Anna , Remer Thomas , Wudy Stefan A , Tomlinson Jeremy W , Grüters Annette , Stewart Paul M , Strasburger Christian J , Quinkler Marcus

Objective: The incidence of childhood obesity and type 2 diabetes has reached epidemic proportions. Glucocorticoid excess causes central obesity and diabetes mellitus as seen in Cushing’s syndrome. The 11beta-hydroxysteroid dehydrogenase type 1 enzyme (11beta-HSD1), which is predominantly expressed in liver and adipose tissue, regenerates active cortisol from inactive cortisone. Increased 11beta-HSD1 may cause tissue-specific Cushing syndrome with central obesity and impa...

ea0014p204 | (1) | ECE2007

Clinical presentation of nonclassic congenital adrenal hyperplasia &br;(NC-CAH): from suspicion to diagnosis

Román Anna Casteràs , Fernández Ma Concepción Páramo , Aguado Javier De la Fuente , Abad Elena Hervás , Rodríguez Laura Fajar , Gil Beatriz Mantiñán , Cano Reyes Luna , Iraeta Ma Antonia Rego , Rodríguez Digna , Lestón Domingo González , García-Mayor Ricardo Víctor

Background: Nonclassic congenital adrenal hyperplasia (NC-CAH) caused by mutations in CYP21B gene is an inherited disorder with various clinical forms in relation to the 21-hydroxylase (21OH) activity. Classic forms are recognized early during neonatal period as salt-wasting crisis or genital ambiguity, while non-classic form presents later with wide hyperandrogenic spectrum. Genetic testing has proved to be the definitive diagnostic method.Aim: To obser...

ea0014p205 | (1) | ECE2007

Daily and nightly urinary free cortisol ratio as a marker of the hypothalamic-pituitary-adrenal (HPA) axis activity in abdominal obesity

Vicennati Valentina , Pagotto Uberto , Ambrosi Bruno , Maccario Mauro , Uberti Ettore Degli , Boscaro Marco , Cavagnini Francesco , Mantero Franco , Armanini Decio , Vettor Roberto , Marugo Mario , Pasquali Renato

Abdominal obese (AO) women might have a hyperactivation of the HPA axis. The limitations of previous studies have been often represented by the limited and heterogeneous number of patients enrolled. Our aim was to asses urinary free cortisol (UFC) output during daily and nightly hours in a large cohort of AO women vs. normal weight controls (CT). 107 AO women and 37 CT were enrolled in the study. In basal condition, each subject underwent OGTT, biochemical determinations. Each...

ea0014p206 | (1) | ECE2007

Interaction of hypothalamic receptors involved in weight regulation

Rediger Anne , Tarnow Patrick , Grüters Annette , Schäfer Michael , Biebermann Heike

Objectives: Food intake is centrally regulated in hypothalamic nuclei where many G-protein-coupled receptors (GPCRs) are expressed which are known to be involved in weight regulation. Peripheral hormonal signals activate their corresponding receptors in the arcuate nucleus. Orexigenic signals activate POMC expression in one subset of neurons and inhibit AgRP and NPY expression in a second subset. Cleavage products of POMC, α- and β-MSH then stimulate melanocortin-4-r...

ea0014p207 | (1) | ECE2007

Association of estrogen receptor-alpha gene polymorphisms with cerebrovascular disease in patients with metabolic syndrome

Xita Nektaria , Markoula Sophia , Lazaros Leandros , Kyritsis Athanasios , Georgiou Ioannis , Tsatsoulis Agathocles

Introduction: The vascular protective effects of estrogens are known to be mediated by their binding to specific estrogen receptors (ER). However, the significance of genetic variations of the ER in vascular diseases has not been reported. We have examined the association between stroke and PvuII and XbaI polymorphisms of the estrogen receptor-alpha gene in patients with metabolic syndrome.Methods and subjects: The study population consisted by 84 male a...

ea0014p208 | (1) | ECE2007

Bone mineral density and body composition in pubescent obese children endangered by metabolic syndrome

Tobisch Borbala , Kozma Andrea , Nagy-Szakall Zsuzsanna , Peter Ferenc , Blatniczky Laszlo

Aims: The prevalence of type 2 diabetes due to insulin resistance is increasing in puberty. Authors have investigated whether body weight excess arises only from increased fat content of the body. Different anthropometrical data were also analysed if they are able to predict degree of insulin resistance.Materials and methods: 108 obese children (50 female, 58 male, Tanner st-s1-5, mean age 12.06 years, mean BMI: 30.97 kg/m2) with positive fami...

ea0014p209 | (1) | ECE2007

Influence of gaining weight on metabolic syndrome in the menopause

Simoncig-Netjasov Aleksandra , Vujovic Svetlana , Stojanovic Milos , Ivovic Miomira , Tancic Milina , Drezgic Milka

Metabolic syndrome (MS) represents a prominent risk factor for cardiovascular disease. Parameters of MS were compared between obese women and controls. I: 50 women (31.92±5.83 kg/m2; 54.4±3.64 y’s); Controls: 37 women (23.50±2.13 kg/m2; 53.92±3.95 y’s). Weight, height, waist and hips circumference, sagital abdominal diameter (SAD) and blood pressure (BP) were measured. Blood was taken at 8 am for: fasting glucose, triglycerid...

ea0014p210 | (1) | ECE2007

Effects of physiological bell-shaped elevations of free fatty acids on glucose metabolism and insulin sensitivity in humans

Gormsen Lars , Nielsen Charlotte , Jessen Niels , Vestergaard Esben , Christiansen Jens , Jørgensen Jens , Møller Niels

Background: Physiological elevations of free fatty acids (FFAs) occur in a dynamic bell-shaped fashion lasting some hours, e.g. nocturnally and during exercise. In order to define the metabolic role of physiological elevations in relation to diurnal fluctuations in insulin sensitivity, the present study was designed to identify the metabolic effects of a dynamic 4 hour elevation of FFAs during a glucose clamp.Materials and methods: 8 lean, healthy men we...

ea0014p211 | (1) | ECE2007

The metabolic changes induced by glucocorticoids: involvement of AMP-activated protein kinase

Christ-Crain Mirjam , Kola Blerina , Lolli Francesca , Fekete Csaba , Seboek Dalma , Wittman Gabor , Ajodha Sharon , Harvey-White Judith , Kunos George , Mueller Beat , Amaldi Giorgio , Giacchetti Gilberta , Boscaro Marco , Grossman Ashley , Korbonits Marta

Background: Excess glucocorticoids result in Cushing’s syndrome (CS) which is characterised by increased food intake, central obesity, dyslipidaemia and insulin resistance, leading to the metabolic syndrome. AMPK is a regulator of energy homeostasis and plays an important role in the regulation of appetite, glucose uptake, lipogenesis and gluconeogenesis. We hypothesised that the effects of corticosteroids on metabolism would be mediated by changes in AMPK activity in a t...

ea0014p212 | (1) | ECE2007

Net endogenous acid production and circulating leptin are associated with potentially bioactive free glucocorticoids in healthy lean women

Maser-Gluth Christiane , Dimitriou Triantifillia , Remer Thomas

Recent evidence suggests that endogenous glucocorticoids (GC) may be suppressed by adipocyte-derived leptin and elevated by dietary acidity. Therefore we examined whether these factors might be predictors of potentially bioactive free glucocorticoids independently of adrenocortical activity.Body composition, plasma cortisol, plasma leptin, 24-h urinary excretion rates of net acid (NAE) reflecting daily diet-dependent acid load, total nitrogen, urinary fr...

ea0014p213 | (1) | ECE2007

Serum gamma-glutamiltransferase increases in type 2 diabetes mellitus but it is not related with the body mass index

Ferrer-García Juan Carlos , García-Fabra Xelo , Albalat-Galera Raquel , Sánchez-Llópez Patricia , Herrera-Ballester Agustín , Juan Carlos Sánchez

Objectives: We have examined the relationship between the hepatic enzymes and type 2 diabetes. We have analyzed if the levels of hepatic enzymes are associated with body weight, lipid profile and the treatment with metformin, thiazolidinediones or statins.Methods: 318 patients with type 2 diabetes were included and they were compared with 100 healthy subjects. Alanine aminotransferase (ALT), aspartate aminotransferase (AST) and gammaglutamiltranspeptidas...

ea0014p214 | (1) | ECE2007

Effects of pharmacological stimulation or blockade of cannabinoid receptor type 1 (CB1) on gene expression in mouse cultured adipocyte cells

Bellocchio Luigi , Cervino Cristina , Fanelli Flaminia , Vicennati Valentina , Pasquali Renato , Pagotto Uberto

The endocannabinoid system has recently emerged as an important modulator of several functions of adipose tissue by altering cell proliferation and gene expression. In this work, we investigated the effects of CB1 activation/blockade in mouse 3T3-L1 adypocite cells by using WIN55,212, a CB1/CB2 agonist and rimonabant, a specific CB1 antagonist, in different experimental settings such as acute treatment on pre-adipocytes and on mature adipocytes, and chronic treatment during di...

ea0014p215 | (1) | ECE2007

Absence of TSH-induced increase in leptin levels in patients with history of differentiated thyroid carcinoma undergoing rhTSH testing

Cecoli Francesca , Andraghetti Gabriella , Ghiara Carla , Briatore Lucia , Cavallero Domenico , Mussap Michele , Minuto Francesco , Giusti Massimo

Some extra-thyroid effects of TSH have been described in vitro and in vivo. TSH has recently been suggested to induce IL-6 secretion from adipocytes. Leptin is the main secretory protein from adipose tissue. Our aim was to evaluate the acute effect of rhTSH-induced TSH surge on serum leptin levels in differentiated thyroid carcinoma (DTC) patients. Ten patients (2 m, 8 f; age range 31–66 years) with stage 1-3 DTC were evaluated during scheduled standard rhTS...

ea0014p216 | (1) | ECE2007

Serum visfatin in relation to insulin resistance and markers of hyperandrogenism in lean and obese women with polycystic ovary syndrome

Kowalska Irina , Straczkowski Marek , Nikolajuk Agnieszka , Adamska Agnieszka , Karczewska-Kupczewska Monika , Otziomek Elzbieta , Wolczynski Slawomir , Gorska Maria

Visfatin is a newly discovered protein secreted by adipose tissue, which is suggested to play a role in pathogenesis of insulin resistance. Polycystic ovary syndrome (PCOS) is heterogeneous disorder, where insulin resistance might be involved in the development of endocrine and metabolic abnormalities. The aim of the present study was to asses the relation between serum visfatin and insulin sensitivity and markers of hyperandrogenism in a lean and obese PCOS patients. The stud...

ea0014p217 | (1) | ECE2007

Relationship between serum adiponectin and oxidative and non-oxidative glucose metabolism in apparently healthy humans

Adamska Agnieszka , Straczkowski Marek , Kowalska Irina , Nikolajuk Agnieszka , Karczewska-Kupczewska Monika , Karolczuk-Zarachowicz Malgorzata , Otziomek Elzbieta , Gorska Maria

The pathogenesis of insulin resistance is not completely understood, however, there are data that it might be associated with altered tissue carbohydrate and lipid oxidation. Adiponectin may be a key regulator of insulin sensitivity and energy metabolism. The aim of the present study was to determine the association of adiponectin, glucose metabolism (oxidation and storage) and lipid oxidation by applying the euglycemic clamp technique and indirect calorimetry.<p class="ab...

ea0014p218 | (1) | ECE2007

Relationships between serum adiponectin, interleukin 10 and interleukin 18 concentrations and muscle lipid fractions in healthy humans

Straczkowski Marek , Kowalska Irina , Nikolajuk Agnieszka , Baranowski Marcin , Adamska Agnieszka , Karolczuk-Zarachowicz Malgorzata , Karczewska-Kupczewska Monika , Zabielski Piotr , Blachnio Agnieszka , Gorski Jan , Gorska Maria

Intramuscular lipids, including ceramide, might be responsible for the development of insulin resistance. Insulin action is also inversely associated with circulating proinflammatory cytokines, like interleukin (IL)-18 and positively related to antiinflammatory factors, like adiponectin and IL-10. The aim of the present study was to estimate the relationships between serum adiponectin, IL-10 and IL-18 concentrations and muscle lipid fractions in healthy humans.<p class="ab...

ea0014p219 | (1) | ECE2007

Prevalence of metabolic syndrome in old men and its relation to ghrelin

Alfaro Sergio Rueda , Serra-Prat Mateu , Fernández Cristián Fernández , Palomera Elisabet , Casamitjana Roser , Domingo Manel Puig

Aim: To study the prevalence of metabolic syndrome (MS) and its relation with ghrelin in old men.Material and methods: Prospective-population based study (2002–2005) in which 153 independently living men older than 70 y were included. Comorbidities, physical exam, BMI, blood pressure were recorded and blood sample taken for biochemical and hormonal determinations. Metabolic syndrome was defined using IDF criteria.Results: MS w...

ea0014p220 | (1) | ECE2007

Antipsychotic drugs and associated metabolic disorders

Melinda Kolcsár , Réka Nagy , Imre Nagy , Annamária László , Zoltán Kun Imre

Obesity is a major contributor to a range of metabolic disorders responsible for much of the medical morbidity and mortality. Increasing numbers of reports concerning not only obesity, but diabetes, hyperglycaemia and lipid dysregulation in patients treated with antipsychotics have raised concerns about a possible association between these metabolic effects and treatment with these medications. The objective of our study was to investigate the prevalence of obesity and other m...

ea0014p221 | (1) | ECE2007

Associations between thyroid function parameters and adipokines in euthyroid individuals

Bajnok Laszlo , Seres Ildiko , Varga Zsuzsa , Jeges Sara , Peti Attila , Karanyi Zsolt , Juhasz Attila , Csongradi Eva , Mezosi Emese , Nagy Endre V , Paragh Gyorgy

Adipose tissue secreted hormonal mediators, adipokines play pivotal roles in the regulation of, among others, central nervous and immune systems influencing body weight, insulin action and inflammatory responses. The aim of our present study was to investigate possible associations of thyroid function with plasma levels of three of known adipokines, i.e. leptin, adiponectin and resistin in 74 Caucasian subjects without any endocrine diseases or related therapy. In order to cre...

ea0014p222 | (1) | ECE2007

Human adipose tissue derived DPP-IV regulates lipolysis through NPY in cultured abdominal subcutaneous adipocytes

Kos Katarina , Baker Adam , Harte Alison , McTernan Philip , O’Hare Paul , Kumar Sudhesh

We have previously shown that the orexigenic hormone NPY is secreted by human adipocytes. The orexigenic hormone NPY(1–36) is truncated by the dipeptidyl-inhibitor IV (DPP-IV) to NPY(3–36) as consequence its affinity changes from receptor Y1 to Y4 and 5. The aim was to investigate whether DPP-IV is expressed in adipose tissue (AT) where it could modulate adipose tissue growth through modulation of NPY activity. This is relevant in light of DPP-IV inhibitors utilised ...

ea0014p223 | (1) | ECE2007

The role of nitric oxide in pathogenesis of development of arterial hypertension during obesity

Giorgadze Diana , Giorgadze Ellen , Doliashvili Tamar , Sanikidze Tamar , Asatiani Ketevan , Bochorishvili Ketevan , Surmava Arkadi

Background: The cardiovascular complication is the main cause of morbidity and mortality in obese patients. Endothelial dysfunction and atherosclerosis have the goal role in development of these diseases. The aim of our study was to reveal the role of nitric oxide during obesity associated arterial hypertension.Subject and method: 200 obese patients (age 35–55) were investigated. Control group comprised 25 healthy subjects. We calculated BMI, determ...

ea0014p224 | (1) | ECE2007

Effects of PGC-1α on endothelial function and apoptosis

Park Joong-Yeol , Won Jong Chul , Lee Ki-Up , Lee Woo Je , Ko Kyung Soo , Song Kee-Ho , Lee In-Kyu

Aims: Central obesity is associated with increased cardiovascular morbidity and mortality. It has been proposed that increased lipid accumulation in vascular tissue and the consequent increase in oxidative stress may be a missing link between obesity and atherosclerosis. The peroxisome proliferators-activated receptor (PPAR)-γ coactivator 1-α (PGC-1α) is a transcriptional coactivator playing an important role in energy metabolism. PGC-1α is present in vascu...

ea0014p225 | (1) | ECE2007

Changes in serum glucose metabolism and growth hormone, cortisol, prolactin, ghrelin, leptin concentrations in normal weight patients with schizophrenia before treatment with atypical antipsychotics

Doknic Mirjana , Maric Nadja , Pekic Sandra , Damjanovic Aleksandar , Miljic Dragana , Stojanovic Marko , Djurovic Marina , Jasovic-Gasic Miroslava , Dieguez Carlos , Casanueva Felipe , Popovic Vera

Schizophrenia is a devastating mental illness associated with obesity and diabetes mellitus rates that far exceed those of the general population.The aim was to evaluate changes in positive energy balance (serum insulin, leptin and ghrelin) and hormones involved in neuroendocrine regulations (serum cortisol, growth hormone and prolactin) before treatment with atypical antypsyhotics (SGA) in normal weight patients with schizophrenia.<p class="abstext"...

ea0014p226 | (1) | ECE2007

Frequency of hypogonadism in males with type 2 diabetes and its relation with erectile dysfunction and obesity

García-Malpartida Katherinne , Casañ-Fernández Rosa , Yanini-García Jesús , Jover-Fernández Ana , Gómez-Balaguer Marcelino , Solá-Izquierdo Eva , Morillas-Ariño Carlos , Hernández-Mijares Antonio

Introduction: The aim of our study was to determine the frequency of hypogonadism (H) in males with type 2 diabetes (T2D) and its relation with erectile dysfunction (ED) and obesity.Methods: We studied 107 diabetic males who came successively to an Endocrine consultation. The presence of H was determined by total testosterone (T) with an immunofluerescence method and free testosterone (fT) calculated with Vermeulen’s equation, defining H if T<2 ...

ea0014p227 | (1) | ECE2007

Acute phase reactants and soluble cell adhesion molecules are associated to plasma leptin levels in obese nondiabetic children

Garanty-Bogacka Barbara , Syrenicz Malgorzata , Syrenicz Anhelli , Gebala Aneta , Dawid Grazyna , Walczak Mieczyslaw

There are increasing evidences that leptin, a protein secreted by adipose tissue, may be an important factor contributing to the development of atherosclerosis. In this study, the relationship between plasma leptin levels and markers of inflammation and endothelial activation was investigated in 214 obese nondiabetic children and adolescents. Fasting levels of leptin, C-reactive protein (CRP), fibrinogen (FB), interleukin-6 (IL-6), interleukin-1β (IL-1β), intercellul...

ea0014p228 | (1) | ECE2007

Relationship between homocysteine level and low-grade systemic inflammation in obese children with metabolic syndrome

Syrenicz Justyna , Garanty-Bogacka Barbara

Obesity is an independent risk factor for the development of cardiovascular disease, frequently associated with various metabolic disorders defined as metabolic syndrome. High plasma total homocysteine (Hcy) concentration is now well established as a powerful risk indicator for a wide range of vascular diseases.The aim of this study was to investigate total Hcy levels in obese children and their possible association with both metabolic syndrome and vario...

ea0014p229 | (1) | ECE2007

Oxidative stress and antioxidant defense is associated with adiposity in men among the urban population of south Iran

Haghparast Fariborz , Nourooz-Zadeh Jaffar

Introduction: Changes in lifestyle have resulted in an increased number of boese subjects, and obesity is currently an important causative factor of health-related problems in Iran.Aims: To investigate the direct relationship of oxidative stress and antioxidant status with obesity in men.Materials and methods: We measured the plasma levels of malondialdehyde (MDA) as a marker of oxidative stress and vitamin E, glutathione and super...

ea0014p230 | (1) | ECE2007

Ghrelin basal levels in metabolic syndrome

Mokhort Tatiana

Ghrelin is known to play an important role in overweight formation and glucose metabolism regulation. The aim was to assess ghrelin basal secretion features in persons with metabolic syndrome (MS).We examined 39 patients (age 35–55years) with MS (IDF criteria) and 28 healthy persons of comparable age. Ghrelin, insulin and C-peptide serum concentrations were measured by immunoenzyme method, lipid spectrum parameters - by spectrophotometry. For IR ass...

ea0014p231 | (1) | ECE2007

Pioglitazone treatment significantly decreases 5-alpha reductase activity and improves metabolic risk factors in PCOS

Glintborg Dorte , Hermann Anne Pernille , Hagen Claus , Veldhuis Johannes , Schmedes Anne , Frystyk Jan , Flyvbjerg Allan , Andersen Marianne

Objective: To investigate the effect of pioglitazone on cortisol metabolism in PCOS.Design: Thirty insulin resistant PCOS patients were randomized to either 16 weeks of pioglitazone (30 mg/day) or placebo treatment. Before and after intervention, patients underwent 24 h 20 min.-integrated blood sampling for measurement of cortisol and 24 h excretion of cortisol, cortisone and steroid metabolites (cortisol, corticosteron, androgen, and 17-hydroxyprogester...

ea0014p232 | (1) | ECE2007

Plasma adiponectin and leptin levels in menopausal metabolic syndrome

Virsaladze David , Adamia Nona , Charkviani Nino , Khitarishvili Ketevan , Giorgadze Tea , Tananashvili David

Objective: The aim of our investigation was the study of blood adiponectin and leptin levels in patients with menopausal metabolic syndrome (MMS) and their correlation with the parameters of MMS features.Methods: 40 females with menopausa have been investigated. In 38 cases diabetes mellitus type 2 has been registered, and in 2 – impaired glucose tolerance. Mean duration of postmenopausal period was 11.1±7.4. Control group consisted of 10 femal...

ea0014p233 | (1) | ECE2007

Serum interleukin 6 and soluble form of interleukin 6 receptor concentrations in obese subjects with impaired glucose tolerance

Nikolajuk Agnieszka , Straczkowski Marek , Kowalska Irina , Adamska Agnieszka , Kinalska Ida , Gorska Maria

Background: Obesity is associated with an increased risk of impaired glucose tolerance and type 2 diabetes. Insulin resistance is the link between obesity and disturbances of glucose metabolism. It is suggested that some substances secreted by adipose tissue might play a role in the pathogenesis of insulin resistance. One of these substances is interleukin-6 (IL-6), cytokine, which regulates synthesis of the acute-phase proteins in the liver. The aim of the present study was t...

ea0014p234 | (1) | ECE2007

Association of sex hormone-binding globulin (shbg) levels with measures of adiposity and metabolic profile in apparently healthy individuals

Saltiki Katerina , Voidonikola Paraskevi , Stamatelopoulos Kimon , Mantzou Emily , Chryssohoou Eleftheria , Papamichael Christos , Alevizaki Maria

Objective: The association of SHBG levels with obesity, hyperinsulinemia and metabolic abnormalities is well recognized in both men and women. Recent data suggest that SHBG levels are an important predictor of cardiovascular disease (CVD) risk. Several methods have been used for the measurement of adiposity including ultrasonography (U/S) which is a reliable and low-cost method. We used U/S to assess regional adiposity and investigated possible associations with SHBG levels.</...

ea0014p235 | (1) | ECE2007

Comparative analysis of adiponectin, leptin and C-peptide levels in obese non-diabetic, type 1 diabetic and lean non-diabetic children

Sysoyeva Olga , Karlovich Nataly , Mokhort Tatiana

A range of hormones which regulate energy metabolism are secreted by adipose tissue, among which adiponectin and leptin are the main adipokines regulating insulin sensitivity.The aim of our study was to estimate and compare levels of adiponectin, leptin, C-peptide and adiponectin-to-leptin ratio (A/L) in obese non-diabetic, Type 1 diabetic (T1D) children and lean non-diabetic controls.BMI and SDS BMI were calculated in 88 children ...

ea0014p236 | (1) | ECE2007

Effects of pioglitazone and metformin on body weight and the insulin resistance parameters in young patients with obesity

OLeksyk Olya , Serhiyenko Alexander , Serhiyenko Victoria

Background and Aims: The aim of this study was to evaluate the effects of pioglitazone (PGZ) and metformin (MET) plus Hypocaloric Diet (HD) in young patients with obesity and impaired oral glucose-tolerance test (75 g glucose).Materials and methods: 49 patients (17.1±1.2 yrs) were allocated in groups: A (n=14) received PGZ 30 mg tid plus HD, B (n=12) - MET 1000 mg tid plus HD, C - PGZ 30 mg plus MET 1000 mg plus HD (n=11), D (<i...

ea0014p237 | (1) | ECE2007

Plasma visfatin levels during oral glucose tolerance test in obese women

Micic Dragan , Sumarac-Dumanovic Mirjana , Kendereski Aleksandra , Cvijovic Goran , Stamenkovic-Pejkovic Danica , Georgiev Maja , Zoric Svetlana , Jorga Jagoda , Dieguez Carlos , Casanueva Felipe

Visfatin is expressed in visceral adipose tissue and is up regulated in some animal models of obesity. Insulin-mimetic actions of visfatin may be part of the feedback regulation of glucose homeostasis, so plasma glucose or insulin may have effect on visfatin levels in humans. The aim of study was to investigate plasma glucose, insulin and visfatin during oral glucose tolerance test (OGTT, 75 gr) in obese women. 22 obese women (age: 36.73±1.88 yrs; BMI 34.72±0.67 kg/m...

ea0014p238 | (1) | ECE2007

The association of high sensitivity C-reactive protein levels with body fat mass and body fat distribution

Gokalp Deniz , Tuzcu Alpaslan , Akay Hatice , Arikan Senay , Bahceci Mithat

Background and aim: C-reactive protein (CRP) is a sensitive marker for systemic inflammation. In this study we aimed to investigate the relationship between high-sensitivity C-reactive protein (hs-CRP) levels and BMI, body fat mass and fat distribution in healthy subjects.Subjects and methods: A total 117 healthy subjects aged with 20–68 yr [normal weight (BMI 18.5–25.0 kg/m2, n:35), overweight (BMI: 25–30 kg/m2</sup...

ea0014p239 | (1) | ECE2007

Innervation of white and brown adipose tissue: dual viral transneuronal tracing study

Dénes Ádám , Darida Miklós , Boldogkoi Zsolt , Kovacs Krisztina

Central control of body weight involves coordinated regulation of food intake and energy metabolism. White (WAT) and brown (BAT) adipose tissue represent functionally distinct compartments of lipid storage and fuel consumption, respectively. Both adipose tissues are innervated by the sympathetic nervous system. Tyrosine hydroxylase positive fibers were found in between fat cells. To determine the extent to which the control of different fat compartments is provided by the same...

ea0014p240 | (1) | ECE2007

Identification of orexin receptors in brown adipocytes: functional effects of orexin-B

Digby Janet , Chen Jing , Markovic Danijela , Viengchareun Say , Lombes Marc , Lehnert Hendrik , Randeva Harpal

Objective: Orexin-A and orexin-B and their G-protein coupled receptors (orexin receptor-1 & -2: OX1R, OX2R) have divergent effects on physiological behaviour, cardiovascular regulation, glucocorticoid and insulin release. Furthermore, orexins have been shown to affect both brown adipose tissue energy expenditure and thermogenesis through stimulation of sympathetic nerve activity. Despite in vivo studies demonstrating a role for orexins acting centrally on adipose ti...

ea0014p241 | (1) | ECE2007

Waist circumference and BMI as predictors of arterial hypertension in childhood and adolescence in Latvia

Madrevica Inese , Krikis Juris , Ligere Renate

Child and adolescent adiposity is a problem of major concern not only for Europe, but also for the world at large. Increase of waist circumference, BMI and arterial blood pressure are metabolic syndrome risk factors which contribute to the development of cardiovascular disease, hypertension and diabetes mellitus.To determine whether changes in arterial blood pressure are related to the increase of waist circumference and BMI in childhood and adolescence....

ea0014p242 | (1) | ECE2007

Uric acid is an important predictor of metabolic disturbances in obese women

Cikim Ayse Sertkaya , Bayraktaroglu Taner , Azezli Adil Dogan , Orhan Yusuf

Introduction: It was shown that a relationship between uric acid and cardiovascular diseases, and hyperuricemia is associated with systemic inflammation. But, determination of uric acid is widely available and inexpensive, it has been overlooked as a marker of systemic inflammation and metabolic disturbances. In this study, we aimed to evaluate uric acid value and its association with inflammation and metabolic disturbances in overweight and obese Turkish women.<p class="a...

ea0014p243 | (1) | ECE2007

Fasting and postprandial plasma obestatin levels are reduced in obesity

Maier Christina , Haider Dominik , Vila Greisa , Riedl Michaela , Luger Anton , Wolzt Michael

Obestatin has recently been identified as a peptide derived from pre-proghrelin that opposes ghrelin effects on appetite and body weight in rodents. We studied the effect of food intake on both these hormones in obese and lean subjects and recorded in parallel the subjective sensations of satiety and hunger. Eight obese (two males and six females, BMI=31–52 kg/m2) and eight age- and sex-matched lean subjects (BMI=19–23 kg/m2) were randomized to ...

ea0014p244 | (1) | ECE2007

Insulin resistance and insulin secretion in morbidly obese patients before and six months after bariatric surgery

Promintzer Miriam , Prager Gerhard , Stadler Marietta , Anderwald Christian , Mandl Martina , Manesch Soheila Shakeri , Nowotny Peter , Bischof Martin Georg , Ludvik Bernhard , Luger Anton , Krebs Michael

Recent case reports describe hyperinsulinemic hypoglycemia after gastric bypass. The aim of this study was the assessment of insulin resistance and insulin secretion in non-diabetic morbidly obese patients before and six months after bariatric surgery.In 8 non-diabetic morbidly obese patients (OB:6f/2 m, age:42±3a, BMI:47.29±2.2 kg/m2) and 6 controls (CON:4f/2 m, age:43±0a, BMI:23.8±0.5 kg/m2) we performed a freq...

ea0014p245 | (1) | ECE2007

Growth hormone Reduces Inflammation in Postmenopausal Women with Abdominal Obesity: a 12-month randomized placebo-controlled trial

Franco Celina , Andersson Björn , Lönn Lars , Bengtsson Bengt-Æke , Svensson Johan , Johannsson Gudmundur

Context: Abdominally obese individuals have relative hyposomatotropism, elevated serum markers of inflammation, and increased risk of cardiovascular disease (CVD).Objective: The aim was to study the effect of GH treatment on serum levels of inflammatory markers and vascular adhesion molecules in postmenopausal women with abdominal obesity.Design: Forty postmenopausal women aged 51–63 yrs with abdominal obesity received GH (0.6...

ea0014p246 | (1) | ECE2007

Hyperactivity of the hypothalamic-pituitary-axis and adrenal hyperandrogenism in polycystic ovary syndrome: a consequence of 5 β-reductase hyperfunction

Gambineri Alessandra , Patton Laura , Forlani Giulia , Munarini Alessandra , Tomassoni Federica , Pagotto Uberto , Pasquali Renato

Among the uncertainties surrounding the etiology of polycystic ovary syndrome (PCOS) the role of increased peripheral cortisol metabolism has become interesting, particularly in relationship to the pathogenesis of adrenal hyperandrogenism. The patways of cortisol metabolism include irreversible inactivation of cortisol by 5α- and 5β-reductase. To evaluate the association of 5α- and 5β-reductase activity with adrenal hyperandrogenism in PCOS, we recruited 90...

ea0014p247 | (1) | ECE2007

An examination of the prevalence of IDF and ATPIII defined metabolic syndrome: towards population based screening

Waterhouse Deirdre , Sharma Jayant , Sheehan Frances , O’Shea Donal

Introduction: Despite the significant associated cardiovascular morbidity, as well as the significant economic implications, little consideration has been given towards population screening for the Metabolic Syndrome (MetS). Therefore, we wished to estimate the prevalence of MetS, using both the recently published IDF, as well as the previously defined Cholesterol Education Program Adult Treatment Panel III (ATPIII), criteria. Additionally, we hypothesised that simple, inexpen...

ea0014p248 | (1) | ECE2007

Neuroendocrine and genetic aspects of metabolic disturbances in women with simple obesity, polycystic ovary syndrome (PCOS) and eating disorders

Baranowska-Bik Agnieszka , Bik Wojciech , Wolinska-Witort Ewa , Gaj Pawel , Mikula Michal , Bragoszewski Piotr , Ostrowski Jerzy , Baranowska Boguslawa

Neuropeptides and adipocytokines influence metabolic homeostasis and food intake. Deregulation in their secretion leads to insulin resistance or metabolic syndrome. Adiponectin possesses anti-diabetic and insulin sensitizing properties. Expression of this gene remains under control of nuclear peroxisome proliferator-activated receptor (PPAR)-gamma. Ghrelin, an endogenous ligand for GH secratagogue receptor (GHSR), modulates metabolic homeostasis. A high amino-acid homology and...

ea0014p249 | (1) | ECE2007

The effect of body composition and iron status on insulin resistance in hemodialysis patients

Rasic Zorica , Perunicic Gordana , Tica Jelena , Popovic Vesna , Vujovic Marina , Gluvic Zoran

Background/Aims: High level of inflammatory cytokines was present within malnourished and chronic renal failure maintenance hemodialysis (MHD) patients, but there were conflicting data about the role of inflammation on development of insulin resistance (IR) in non-obese and overweight MHD patients.Methods: We selected 23 well-nourished and 20 middle- to moderate-malnourished, sex and age-matched, stable MHD patients, 23 male and 20 female, with median di...

ea0014p250 | (1) | ECE2007

Relationship between obesity, insulin resistance and adipokines in morbidly obese women

Madani Rana , Miheisi Nazar , Hashemi Majid , Mohamed-Ali Vidya

Background: Several cytokines and chemokines are released by adipose tissue and associated with insulin resistance. We investigated the systemic and in vitro release of RANTES(Regulated upon Activation Normal T-cell Expressed and Secreted), MCP-1(Monocyte Chemo-attractant Protein-1), adiponectin, leptin and IL-6 from three human adipose tissue depots and their relationship to insulin sensitivity.Methods: Fasting blood samples were taken from obese...

ea0014p251 | (1) | ECE2007

Obesity in pre-school children –new epidemic problem?

Barg Ewa , Joanna Chmielarska , Barbara Adamska , Inna Begar , Agnieszka Konieczna , Malgorzata Skarzynska

In the opinion of the World Health Organization the obesity will become one of four major epidemics of the 21st century. The most important factor for its occurrence is the lifestyle.The aim of the research was to analyse the body mass index (BMI) of pre-school children and their parents and the components of their lifestyle, such as diet, sports and free time activities.Material and Methods: The two-stage study included 537 childr...

ea0014p252 | (1) | ECE2007

Somatostatin receptor subtype 2 inhibits glucagon secretion and regulates glucose homeostasis

Strowski Mathias , Singh Vandana , Grötzinger Carsten , Zacharias Sylvia , Wiedenmann Bertram , Plöckinger Ursula

Objectives: Somatostatin (SST) inhibits glucagon and insulin secretion. Five receptor subtypes for SST are known (SSTR1-SSTR5), all of which are expressed in the endocrine pancreas. SSTR2 inhibits glucagon secretion in vitro, however its role in vivo is not well understood. Here, we characterize the role of SSTR2 in regulating glucose homeostasis in mice with diet-induced obesity.Methods: SSTR2-deficient (SSTR2−/−) and control mice...

ea0014p253 | (1) | ECE2007

The role of combined treatment of arterial hypertension in patients with obesity

Tamar Doliashvili , Ellen Giorgadze , Ketevan Asatiani , Arkadi Surmava

The aim of our study was to assess the advantage of combinative therapy with lisinopril and moxonidin for the treatment of arterial hypertension in group of obese patients in comparison to the single-drug therapy with lisinopril.Methods: 26 obese patients were divided in 2 groups. They underwent a 24-hour monitoring of the arterial pressure and were diagnosed as arterial hypertension II degree (ESH-ESC). In the I group for the purpose of stabiliza...

ea0014p254 | (1) | ECE2007

A registry of GDM in portugal

Ruas Luísa , Rocha Tiago , Dores Jorge , Carvalheiro Manuela

Introduction: A retrospective study of the year 2003, of 1314 women with GDM, was performed.Patients and methods: Two groups according to pre-pregnancy BMI: Go - BMI&gE;30 Kg/m2; Gno BMI<30 Kg/m2. Mean age 32.9±5 years, A1c<6% in both groups. Influence of BMI in different variables was analysed: family history of DM, weight gain during pregnancy; blood pressure, need of insulin, gestation age at the beginning of insulin...

ea0014p255 | (1) | ECE2007

The effects of glucocorticoids on the expression of gluconeogenic and lipogenic enzymes in a rodent model of Cushing’s syndrome

Lolli Francesca , Christ-Crain Mirjam , Kola Blerina , Fekete Csaba , Wittman Gábor , Grossman Ashley B , Korbonits Márta

Background: Cushing’s syndrome results from chronic exposure to excessive levels of glucocorticoids (GC). The clinical manifestations associated with hypercortisolaemia are variable and differ widely in severity, including hypertension, apparent obesity and metabolic aberrations such as diabetes, dyslipidaemia, ultimately leading to changes similar to the metabolic syndrome. We hypothesised that GC might influence the expression of the genes involved in lipogenesis and gl...

ea0014p256 | (1) | ECE2007

HRT in treatment of dislipidemia in women with hypogonadotropic hypogonadism

Zektser Vita , Ilovayskaya Irena , Iliyn Aleksandr , Goncharov Nikolay , Melnichenko Galina

Hypoestrogenemia is associated with dislipidemia and is an independent risk factor for cardiovascular diseases in postmenopausal women. However, there is a cohort of young women with gonadal steroid deficit caused by the disorders of central regulation. Twenty women with hypogonadotropic hypogonadism (HH) were included in group 1. (median age - 29 years and 3 months, median duration of amenorhea - 5 years 3 months, mean BMI - 24.6±6.05 kg/cm2. Women were examined before a...

ea0014p257 | (1) | ECE2007

Rare polimorphism in the intron of human Agouti- related protein gene is associated with obesity

Kapa Ivo , Kalnina Ineta , Pirags Valdis , Schioth Helgi , Klovins Janis

Agouti related protein (AGRP) as a endogenous antagonist of melanocortin 4 receptor plays an important role in regulation of food intake and energy balance being one of the most potent orexigenic factors. We have determined complete sequence of AGRP gene and upstream promoter region in 100 patients with severe obesity (BMI>35). Three previously described polymorphisms were identified: silent mutation G538A in second exon, non-synonymous mutation G772A (rs5030980) and C662T...

ea0014p258 | (1) | ECE2007

The relationship between plasma androgens (testosterone and dehydroepiandrosterone sulfate), insulin resistance and visceral obesity in elderly men

Rabijewski Michal , Papierska Lucyna , Zgliczynski Wojciech

Introduction: In elderly men testosterone and DHEAS deficiency is often observed, also changes of body composition and metabolic disturbances are common disorders.Objectives: The aim of this study was to analyze the association between testosterone and DHEAS deficiency and waist/hip ratio (WHR) and also levels of glucose, insulin, HOMA and FG/FI ratio in elderly men as well as analysis, whether these sex hormones influent on measured parameters separatel...

ea0014p259 | (1) | ECE2007

Prevalence of metabolic syndrome in a cohort of young Mediterranean women with polycystic ovary syndrome and association with clinical and biochemical parameters

Patton Laura , Gambineri Alessandra , Repaci Andrea , Forlani Giulia , Pagotto Uberto , Pasquali Renato

Aim: The purpose of the study was to evaluate the prevalence of the metabolic syndrome (MS) in a cohort of young Mediterranean women with PCOS in reproductive age and to evaluate the association of the MS with clinical and biochemical parameters.Setting: Among 200 PCOS (17-31 years) criteria of MS in accordance with the “NCEP-ATPIII” were used to construct 3 groups: no one criteria, 1 or 2 criteria and 3 or more criteria (affected by MS). All p...

ea0014p260 | (1) | ECE2007

Obesity: diffusion weighted imaging features of brain

Alkan Alpay , Sahin Ibrahim , Keskin Lezzan , Karakas Hakki Muammer , Cikim Ayse Sertkaya , Karaca Zuhal , Turhan Nedim , Firat Ahmet Kemal , Sigirci Ahmet , Ulutas Ozkan

Homeostasis of the body weight is maintaining by the interactions between the different sections of the brain and peripheral tissues. Diffusion weighted magnetic resonance imaging (DWI) is a method that investigates the microscopic motion of the water particules in tissues and also depends on measurement of the signal variations in tissues which is connected with the kinetic energy of the molecules called; molecular diffusion. The purpose of this work was to detect brain diffu...

ea0014p261 | (1) | ECE2007

Hepatic and brain metabolism in young adults with glycogen storage disease type 1

Mandl Martina , Weghuber Daniel , Krssak Martin , Roden Michael , Nowotny Peter , Brehm Atilla , Krebs Michael , Widhalm Kurt , Bischof Martin

Glycogen storage disease type 1 (GSD1) is a rare inherited defect of endogenous glucose production. While children present with severe hypoglycemia the propensity for hypoglycemia may decrease with age in these patients. It was the aim of this study to elucidate the mechanisms for milder hypoglycemia symptoms in grown up GSD1 patients. Four patients with GSD1 (BMI: 23.2±6.3 kg/m2, age: 21±3 yr) and four healthy controls matched for BMI (23.1±3.0 kg/m<...

ea0014p262 | (1) | ECE2007

Body composition and GH status in morbidly obese females before and after laparoscopic silicone adjustable-gastric banding

Savastano Silvia , Colao Annamaria , Rota Francesca , Vuolo Laura , Cristina Savanelli Maria , Cascella Teresa , Orio Francesco , Angrisani Luigi , Lombardi Gaetano , Di Somma Carolina

The GH/IGF-I axis function are reported to ameliorate after weight-loss. Bariatric surgery leads to a significant weight loss in morbidly obese patients. We investigated the relationships between GH/IGF-I axis and body composition in 20 morbidly obese females (BMI: 44.8±4.7; waist circumference (W) 119.5±7.2 cm, age 33.7±11.7 yrs) with a normal glucose tolerance, before and after laparoscopic silicone adjustable-gastric banding (LASGB). The GH axis was evaluated...

ea0014p263 | (1) | ECE2007

The importance of (TAAAA)n polymorphism of SHBG gene in the metabolic syndrome

Xita Nektaria , Milionis Charalambos , Georgiou Ioannis , Elisaf Moses , Tsatsoulis Agathocles

Introduction: Sex hormone binding globulin (SHBG) levels have been associated with the development of the metabolic syndrome. In particular, low SHBG levels have been proposed as an indicator of increased risk for metabolic syndrome in men. The (TAAAA)n repeat polymorphism SHBG gene is believed to affect SHBG levels. In vitro experiments have shown that the allele with 6 TAAAA repeats is associated with decreased transcriptional activity of SHBG gene. The aim of this study was...

ea0014p264 | (1) | ECE2007

Exophthalmos and its relation to adipokines in obese men

Caglayan Sinan , Ozisik Gokhan , Unal Melih , Ozcan Omer , Ozata Metin

Up to date, many studies were performed regarding the relationship between obesity and inflammation, and exophthalmos which is developed in thyroid-associated ophthalmopathy (Graves’ ophthalmopathy). Several studies suggest that transforming orbital preadipocytes into adipocytes may cause exophthalmos because of the inflammation. Therefore, we examined the relationship exophthalmos and obesity which is also called low-grade systemic inflammation. We investigated the relat...

ea0014p265 | (1) | ECE2007

Influence of orlistat on adiponectin levels in obese women

Tzoitou Marianthi , Papadopoulou Evridiki , Papageorgiou Athanasios , Galactidou A , Kita Marina , Kourtis Anargyros , Poulakos Pavlos , Avramidis Avraam

Introduction: Adiponectin is secreted by adipocytes and has been linked to glucose and lipid regulation. Obesity, diabetes and atherosclerosis have been associated with reduced adiponectin levels. Orlistat lowers lipids and improves insulin sensitivity but its effect on other metabolic parameters is not known.The purpose of this study is to evaluate the influence of orlistat on metabolic and hormonal parameters of the adipose tissue.<p class="abstext...

ea0014p266 | (1) | ECE2007

Effect of omega-3 fatty acids on plasma adiponectin levels in Metabolic syndrome subjects

Dimitrov Dimiter

Background: Increased consumption of fish and fish oil as a source of n-3 long chain polyunsaturated fatty acids (n−3 LC-PUFA), mainly eicosapentaenoic acid (EPA, 20:5 n-3) and docosahexaenoic acid (DHA, 22:6 n-3) is often associated with decreased mortality (as well as morbidity) from cardiovascular disease. Treatment with n-3 LC-PUFA augments circulating adiponectin levels via a PPARγ-dependent mechanism in animal models. Given that adi...

ea0014p267 | (1) | ECE2007

The polymorphism of PPAR and susceptibility to atherosclerosis in children with low birth weight (below 2500 g)

Barg Ewa , Skoczynska Anna , Wikiera Beata , Chacka Dorota , Glab Ewa , Lebioda Arleta , Turczyn Barbara

Children, who are born with low birth weight (less 2500 g) are known to have an increased risk of developing lipid disturbances and atherosclerosis in later live.PPAR alpha activity could play a regulatory role in the pathogenesis of hyperlipidemia and a modulatory role in the control of inflammatory response.The aim of this study was to determine whether the presence of polymorphism in gene of peroxisome proliferators-activated re...

ea0014p268 | (1) | ECE2007

Visfatin, adiponectin, leptin and insulin sensitivity in severe obese women with normal and impaired glucose tolerance

Sumarac-Dumanovic Mirjana , Micic Dragan , Georgiev Maja , Stamenkovic-Pejkovic Danica , Jorga Jagoda , Kenedereski Aleksandra , Cvijovic Goran , Zoric Svetlana , Dieguez Carlos , Casanueva Felipe

Visfatin, a new adipokine, facilitates adipogenesis and has insulin-mimetic properties. There are data that hyperglycemia causes an increase in plasma visfatin levels in people with T2DM this increase gets more prominent as the glucose intolerance worsens. The aim of the study was to investigate plasma visafatin, leptin and adiponectin in obese women with normal and impaired glucose tolerance. Thirteen obese women (age: 34.50±2.57 yrs; BMI 35.05±0.57 kg/m2) with norm...

ea0014p269 | (1) | ECE2007

Characterization of the rat homologue of the human neuroendocrine marker secretagogin – new functional implications by in vitro studies

Gartner Wolfgang , Vila Greisa , Daneva Teodora , Koc-Saral Felic , Majdic Otto , Nabokih Anastasia , Luger Anton , Wagner Ludwig

Objective: Establishment of rodent in vitro cell systems for the extension of the functional data about the recently cloned neuroendocrine marker secretagogin.Methods: 1. DNA-cloning; 2. Antibody generation; 3. Immunoblotting and Immunohistochemistry; 4. Cell-transfection; 5. Luciferase Reporter Assays; 6. ELISA.Results: 1. We characterized the rat homologue of human secretagogin (rat secretagogin) and demonstrated the homol...

ea0014p270 | (1) | ECE2007

Tilapia GnRH receptors: signal transduction and internalization rate

Avitan Ayelet , Levavi-Sivan Berta

Multiple subtypes of GnRH receptor (GnRHR) are present in individual vertebrate species. We found two distinct GnRHRs in tilapia, classified as type 1 and 3 (tGnRHR1/3). Amino-acid similarity between the receptors was calculated at 59%, with the different amino acids scattered throughout the receptors. We compared the sequence analysis and signal transduction of the two tGnRHRs, using the human GnRHR type 1 as a control. Sequence analysis revealed that all three receptors exhi...

ea0014p271 | (1) | ECE2007

The relationship between carotid intima-media thickness metabolic and anthropometric parameters in healthy subjects

Akay Hatice , Gokalp Deniz , Arikan Senay , Tuzcu Alpaslan , Bahceci Mithat

Background and aim: Carotid intima-media thickness (IMT), as assessed by B-mode ultrasound, is a commonly used clinical marker that reflects systemic burden of atherosclerosis and value of IMT at or above 1 mm at any age is associated with a significantly increased risk of myocardial infarction. On the basis of such findings, we aimed to clarify the relationship between carotid intima-media thickness and anthropometric and metabolic parameters in virtually healthy subjects.</p...

ea0014p272 | (1) | ECE2007

CRF and the Urocortins activate NFAT and induce catecholamine production in PC12 cells

Dermitzaki Erini , Tsatsanis Christos , Androulidaki Ariadne , Venihaki Maria , Gravanis Achille , Margioris Andrew

We have previously shown that Corticotropin – releasing factor (CRF) and Urocortins (UCNs) induce the production of catecholamines from normal human and rat adrenal chromaffin and PC12 pheochomocytoma cells via induction of tyrosine hydroxylase, the rate limiting enzyme of catecholamine biosynthesis. We have also shown that CRF induces calcium ion entrance into the cytoplasm from both extracellular sources (influx) and from intracellular stores (mobilization) in PC12 cell...

ea0014p273 | (1) | ECE2007

Gs-dependent receptor endocytosis of melanocortin-4 receptors

Breit Andreas , Heling Dominik , Gudermann Thomas

Melanocortin receptors (MCR), which belong to the superfamiliy of G protein-coupled receptors (GPCR), are preferentially coupled to Gs proteins and play a major role in the regulation of energy homeostasis. In line with this notion, mutations in the MC4R gene are the most frequent monogenic cause of severe obesity in human beings. Recently it has been shown that the MC4R receptor undergoes, similar to most GPCR, GPCR kinase (GRK) and arrestin-mediated ligand-promoted receptor ...

ea0014p274 | (1) | ECE2007

The endocrine disruptor DDT appears to be an uncompetitive inverse agonist for activating TSHr mutants, FSH receptor and LH receptor

Montanelli Lucia , Ferrarini Eleonora , Dimida Antonio , Rossi Mario , Agretti Patrizia , Giorgi Franco , Corsini Giovanni Umberto , Pinchera Aldo , Vitti Paolo , Maggio Roberto , Tonacchera Massimo

The insecticide DDT has been shown to inhibit both the basal and the TSH stimulated accumulation of cAMP in CHO stably transfected with the TSHr (CHO-TSHr). Aim of this study was to evaluate whether the DDT has a similar effect on cells transfected with TSHr mutants displaying a high level of constitutive activity. In addition we investigate the effect of DDT on cells transfected with wtFSHr and wtLHr which share a high degree of amino-acid homology sequence with wtTSHr. In co...

ea0014p275 | (1) | ECE2007

Effect of vitamin D replacement on endothelial function and oxidative stress in vitamin D deficient subjects

Tarcin Ozlem , Yavuz Dilek , Toprak Ahmet , Telli Ahu , Yuksel Meral , Yazici Dilek , Sancak Seda , Deyneli Oguzhan , Haklar Goncagul , Akalin Sema

Introduction: Vitamin D (Vit D) receptors have been shown in extra skeletal tissues. Vit D deficiency plays a role in the development of many malignant, chronic inflammatory, autoimmune and metabolic diseases. Our aim was to evaluate the effect of Vit D replacement therapy on insulin sensitivity, endothelial function and oxidative stress in Vit D deficient subjects.Material-method: Serum 25(OH) D levels of 74 volunteer-healthy subjects (22.7±2.7) we...

ea0014p276 | (1) | ECE2007

Transthyretin is up-regulated by androgens in mice liver and choroid plexus

Santos Cecilia RA , Henrique Alves C , Saraiva Maria Joao , Abreu Raquel , Baltazar Graça , Socorro Silvia , Quintela Telma P , Gonçalves Isabel MT

Transthyretin (TTR) is well documented as a carrier for thyroid hormones. It also binds retinol binding protein preventing its filtration trough the kidneys and therefore is involved in delivering retinol to target cells. Moreover, TTR sequesters amyloid-beta impairing its deposition in nervous tissues and possibly contributing to its removal. Despite its importance in mammalian physiology, there are few studies regarding the regulation of TTR synthesis. In silico analy...

ea0014p277 | (1) | ECE2007

Adrenal incidentalomas: aberrant expression of hormone receptors (preliminary results)

Mena Elena , Sahun Manel , Alià Pedro , Navarro M Ángel , Soler Joan , Villabona Carles

Background: In some patients with adrenal tumors cortisol regulation may be under the control of abnormal or ectopic hormone receptors. The objective of this study is investigate the presence of these aberrant receptors in subjects with adrenal incidentaloma and biochemical criteria of subclinical hypercortisolism.Patients and methods: We studied seventeen patients with adrenal incidentalomas, ten patients with a unilateral tumor (age 48–70, M/F: 4/...

ea0014p278 | (1) | ECE2007

SMP30 is expressed in rat mammary gland and down-regulated by estradiol

Maia Cláudio JB , Santos Cecilia RA , Schmitt Fernando , Socorro Silvia

The SMP30 (Senescence Marker Protein 30) is involved in the maintenance of intracellular Ca2+ homeostasis and in the regulation of various Ca2+- dependent proteins. A suppressive effect on cell proliferation, DNA synthesis and on the expression of oncogenes in rat hepatoma cells overexpressing SMP30 has been reported recently suggesting it may have a role in cancer progression. High levels of SMP30 expression have been found in liver and kidney of rats bu...

ea0014p279 | (1) | ECE2007

Family mutation of PRKRA1A associated with Cushing syndrome from pigmented micronodular adrenal dysplasia

Gentil Alfonso , Martin Tomas , Díaz Galvez Milagrosa , Maraver Silvia , Lucas Miguel

Introduction: Pigmented micronodular adrenal dysplasia (PMNAD) is an infrequent cause of Cushing syndrome ACTH-independent, and can form a part of Carney syndrome (CS). In both, regulatory subunit mutations of the protein kinase A (PRKAR1A) have been demonstrated, but without apparent genotype-phenotype correlation.Objective: To demonstrate the mutation of PRKAR1A and its functional and clinical expression in a family affected with PMNAD.<p class="ab...

ea0014p280 | (1) | ECE2007

Non-genomic glucocorticoid effects on insulin secretion

Vila Greisa , Wagner Ludwig , Nabokikh Anastasia , Luger Anton

Glucocorticoids act directly on pancreatic islets suppressing insulin secretion from the beta cells through a genomic mechanism of slow onset. We present here data on immediate actions of dexamethasone on two models of insulin secretion: RINm5F and INS-1 beta cell lines. Under normal glucose concentrations, dexamethasone rapidly (within minutes) decreased insulin secretion about 30%. Under hypoglycaemic conditions (glucose reduced to 50% for 1 hour) dexamethasone increased ins...

ea0014p281 | (1) | ECE2007

The androgen receptor in the rat choroid plexus

Alves CH , Socorro S , Gonçalves IMT , Santos CRA

The choroid plexus (CP) produces cerebrospinal fluid (CSF) and forms the blood-CSF barrier, being involved in the maintenance of the extracellular milieu of the brain and secretion of several neuroprotective factors. There are several experimental evidences showing that androgens enhance cognition and act as potential protective factors against Alzheimer’s Disease. It has been shown that testosterone exerts neuroprotective actions against oxidative stress, apoptosis, and ...

ea0014p282 | (1) | ECE2007

Correlation of BclI, N363S and the ER22/23EK polymorphisms of the glucocorticoid receptor gene and bone mineral density in patients with endogenous and exogenous hypercortisolism

Szappanos Ágnes , Toke Judit , Boyle Belema , Majnik Judit , Varga Ibolya , Gláz Edit , Tóth Miklós , Rácz Károly

Objective: Genetic variation in the glucocorticoid receptor (GR) gene may be related to the clinical heterogenety and severity of the Cushing’s syndrome. BclI, N363S and ER22/23EK polymorphisms are the three most investigated polymorphisms within the GR gene, however, the importance and magnitude of their effect in hypercortisolemic states are unclear. The BclI and the N363S variants are associated with increased, while the ER22/23EK variant is associated with reduced glu...

ea0014p283 | (1) | ECE2007

In vitro effects of 17 βE2 and raloxifene on desmoid tumour derived cells

Picariello Lucia , Sala Silvia Carbonell , Martineti Valentina , Gozzini Alessia , Brandi Maria Luisa , Tonelli Francesco

Desmoid tumours (DT) are a benign manifestation of familial adenomatous polyposis. The prevalent development in young fertile women, the regression during menopause or with tamoxifen treatment, underlie the potential role of estrogens and Estrogen Receptors (ERs) in the pathogenesis of these tumors. To investigate this hypothesis, the expression of ERs α and β in desmoid tumors derived cell cultures, the effects of 17βE2 and of raloxifene on DT cell p...

ea0014p284 | (1) | ECE2007

ESR2 genotypes are associated with a reduced relative risk for sporadic colorectal cancer

Martineti Valentina , Picariello Lucia , Del Monte Francesca , Valanzano Rosa , Strigoli Debora , Sala Silvia Carbonell , Canonico Giuseppe , Tonelli Francesco , Brandi Maria Luisa

According to incidence data from Italian cancer registers, colorectal cancer is the third most common cancer in both men and women even considering skin non-melanoma cancers, lung and breast cancers. Moreover it represents the third absolute leading cause of cancer death in women and the fourth in men. Although data on Italian population regarding the role of estrogens in colorectal cancer have not yet been collected, several strands of evidence from international epidemiologi...

ea0014p285 | (1) | ECE2007

Frequency of three major glucocorticoid receptor gene polymorphisms in patients with adrenal incidentalomas

Antic Jadranka , Cokic Bojana Beleslin , Ognjanovic Sanja , Vignjevic Jovana , Mirkovic Katarina , Nikolic Jelena , Paunovic Ivan , Damjanovic Svetozar

Background: Several polymorphisms of glucocorticoid receptor (GR) gene, including BclI, N363S and ER22/23EK, which may have an influence on glucocorticoid sensitivity, have been reported. BclI and N363S polymorphisms have been associated with clinical characteristics of increased and ER22/23EK of decreased glucocorticoid (GC) effects. On the other hand, metabolic syndrome has been described in patients with adrenal incidentalomas.Objective ...

ea0014p286 | (1) | ECE2007

An analysis on delays in diagnosis of papillary thyroid cancer

Dionigi Gianlorenzo , Dionigi Renzo , Bartalena Luigi , Tanda Maria Laura , Piantanida Eliana , Boni Luigi , Rovera Francesca

Background: Thyroid carcinoma represents the most frequent form of cancer of the endocrine glands. In Italy, temporal trend shows a significant increase of incidence rates. Papillary thyroid cancer is the most common thyroid malignancy. Papillary thyroid carcinoma happens to be a multicentric tumor and trends to spread to the lymph nodes in the early stage of the disease. Thus early diagnosis is vital to improve the outcome for patients with thyroid cancer. The aim of this stu...

ea0014p287 | (1) | ECE2007

Post-treatment effects of maternal hypothyroidism and thyroxin therapy on the subiculum neuronal density of the newborn rats

Behnam-Rassouli Morteza , Delshad Zahra , Fazel Alireza

Studies in mice and rats suggest that legions of hippocampus interfere with memory for space and context and can have a significant effect on memory storage. The goal of the present study was to investigate the effect of maternal hypothyroidism, and thyroxin therapy on the neuronal density of the subiculum.Twenty five adult female Wistar rats were divided into experimental groups (Exp) 1 and 2 and control. The Exp groups made hypothyroid (500 mg/l PTU in...

ea0014p288 | (1) | ECE2007

Abstract unavailable...

ea0014p289 | (1) | ECE2007

The effects of subclinical hypothyroidism and replacement therapy on paraoxonase-1 (PON-1) and common carotis intima media thickness

Kebapcilar Levent , Comlekci Abdurrahman , Tuncel Pinar , Solak Ahmet , Secil Mustafa , Gencel Omur , Yesil Sena

The mechanism of atherosclerosis in patients with subclinical hypothyroidism (sHT), which has been partly attributed to lipid abnormalities, is still controversial. There is substantial evidence that ox-LDL plays an important role during the atherosclerosis process and paraoxonase-1 (PON-1) significantly inhibits generation of lipid peroxidation and thus plays a role in against atherosclerosis. The aim of the study was evaluate qualitative changes in lipoprotein metabolism, hs...

ea0014p290 | (1) | ECE2007

Elevated plasma FABP4 (aP2) levels in hypothyroidism: potential implication for accelerated atherosclerosis

Ozisik Gokhan , Caglayan Sinan , Gultepe Mustafa , Ozata Metin

Context: FABP4 (adipocyte-specific fatty acid-binding protein 4, also known as aP2) is a cytoplasmic lipid chaperon involved in lipid metabolism, glucose homeostasis, and the regulation of inflammatory response. Its expression is limited to adipocytes, macrophages, skeletal muscle, and bronchial epithelia. Recently, a polymorphic allele of the aP2 promoter (−87T→C) has been shown to be associated with decreased FABP4 expression in fat tissue, lowered triglyc...

ea0014p291 | (1) | ECE2007

Markers of REDOX system at autoimmune diseases of thyroid gland

Tea Tsotsonava , Nona Adamia , Irina Chitava

Objective: Oxidative stress is developing by disequilibrium between antioxidative and oxidative mechanisms. In these conditions dysfunction of thyroid gland (TG) has been reported. It is related with deranged biosynthesis of thyroid hormones, in particular, with the absorption of iodine in thyrocytes. The objective of our investigation was to study the impact of oxidative stress on autoimmune diseases (AD) of TG.Methods: 38 patients [group 1 - with diffu...

ea0014p292 | (1) | ECE2007

Selenium and its relation to thyroid antibodies, volume and ultrasound texture

Smutek Daniel , Cermakova Ivana , Jiskra Jan , Potlukova Eliska , Tesar Ludvik

Objective: To find a relation between thyroid parameters (thyroxin serum level, thyroid antibodies, thyroid gland volume and ultrasound texture) and serum level of selenium.Background: Selenium deficiency can lead to a decrease of triiode-thyronine in peripheral tissues. Changes in thyroid hormone production can be reflected in followed thyroid parameters.Methods: In 33 patients ultrasound examination of thyroid gland was performed...

ea0014p293 | (1) | ECE2007

Which prognosis criteria for thyroid anaplastic carcinoma?

Larroumets Guillaume , Somda Frederic , Roche Beatrice , Dejax Catherine , Desbiez Francoise , Kwiatkowski Fabrice , Thieblot Philippe , Tauveron Igor

The prognosis of thyroid anaplastic carcinoma is poor. Yet can prognosis criteria change the therapeutic options?Method: From a series of 26 cases from a single group recorded between 1990 and 2006, we analyzed the outcome after treatment based on surgery, radio and chemotherapy and looked for prognosis criteria.Results: All but one patients died with a mean survival of 273 days (median survival of 130 days). Over 50% of patients h...

ea0014p294 | (1) | ECE2007

Clinical-epidemiological characteristics of thyroid cancer (TC) in the Crimea

Butyrsky Olexandr , Hajiyev Oraz , Bezrukov Oleg

We retrospectively analyzed case reports of patients been operated within 50 yrs. Our investigation demonstrates increasing tendency of TC. Total frequency of TC has increased from 0.76% in 1953–1964 to 8.48% in 2001–2005. Analysis revealed prevalence of TC in women (87.6%), sharp increase of morbidity after 30 years (14.6%), peak of morbidity – after 60 years (27.4%). Frequency of TC among adolescents is not increased (1.6–2.3%) that’s associated with...

ea0014p295 | (1) | ECE2007

Fine needle aspiration biopsy of the thyroid. Cytohistologic correlation: experience in a central military hospital

Nobre Ema Lacerda , Vilar Helena , Jorge Zulmira , Marcelino Mafalda , André Saudade , Guerra Paulo , Lopes Carlos , de Castro João Jácome

Background and aims: Thyroid nodular disease (TND) is a common condition in the general population. Malignant nodules occur in 5% of patients with thyroid nodules. Fine-needle aspiration biopsy (FNAB) is considered to be the most reliable method of differentiating benign and malignant thyroid nodules. The purpose of this study was to assess the accuracy of FNABs performed in our Hospital.Methods: We retrospectively reviewed the medical records of patient...

ea0014p296 | (1) | ECE2007

Thyroid nodules in the elderly: role of ultrasound (US) and ultrasound-guided fine-needle aspiration biopsy (US-FNAB)

Triggiani Vincenzo , Guastamacchia Edoardo , Resta Francesco , Sabba Carlo , Tafaro Emilio

The prevalence of thyroid nodules is increased in the elderly. The aim of this study was to evaluate the accuracy of US and US-FNAB in the diagnosis of thyroid cancer in a population of elderly subjects, all of whose thyroid nodules were subjected to US-FNAB, with no prior selection based on dimensions or echo-pattern. Over a three-year period, 276 consecutive patients (64 males and 212 [76.8%] females), aged 65 to 87 (mean 70±4.4), underwent US evaluation and US-FNAB of ...

ea0014p297 | (1) | ECE2007

Clinical and pathological characteristics of thyroid anaplastic carcinoma: a regional survey in Auvergne

Larroumets Guillaume , Tauveron Igor , Somda Frederic , Roche Beatrice , Desbiez Francoise , Dejax Catherine , Kwiatkowski Fabrice , Thieblot Philippe , Auvergne Groupe Thyroide

Thyroid anaplastic carcinoma (TAC) is rare among thyroid cancers. Few surveys on these diseases are available.Aim: To analyze the clinical and pathological characteristics of TAC in Auvergne, France, from a series of 26 patients among a cohort of over 1500 thyroid carcinomas (i.e. 1.7% of total thyroid cancers).Results: Mean age was 72.1 years (range 42–91 years), with a sex ratio of 19 women to 7 men. A previous history of th...

ea0014p298 | (1) | ECE2007

Abstract unavailable...

ea0014p299 | (1) | ECE2007

Serum n-terminal pro-b-type natriuretic peptide (NT-proBNP) levels in patients with hyper- and hypothyroidism. Hyperthyroidism may affect NT-proBNP levels as independent of cardiac dysfunction

Arikan Senay , Tuzcu Alpaslan , Gokalp Deniz , Bahceci Mithat , Danis Ramazan

Background and aim: It was known that NT-proBNP levels increased in cardiac failure. But, NT-proBNP levels in different thyroid status still unclear. We aimed to evaluate serum NT-proBNP levels in both of hyperthyroid and hypothyroid patients without cardiac insufficiency.Subjects and methods: Thirty nine patients with hyperthyroidism (43.0±16.5 yr), 25 patients with hypothyroidism (35.4±13.9 yr) and 34 ages matched euthyroid subjects (41.4&#17...

ea0014p300 | (1) | ECE2007

Soluble intercellular adhesion molecule-1(sICAM-1) levels and different schemes of Graves’ ophthalmopathy (GO) treatment

Bogomazova Elena , Mokhort Tatjana

Aim: To evaluate the base-line serum sICAM-1 levels among patients with GO and levels sICAM-1 at the end of 6 month follow up after different schemes of GO treatment.Material and methods: 72 patients with GO have been surveyed. Patients have been put into 4 groups depending on spent treatment: 1st group – 26 patients received methylprednisolone per os 1 mg/kg in a combination with plasmapheresis; 2nd group – 14 patients received p...

ea0014p301 | (1) | ECE2007

The influence of smoking upon the incidence of Graves’ disease and severity of Graves’ ophthalmopathy

Zbranca Eusebie , Branisteanu Dumitru , Scurtu Bogdanel , Mogos Voichita

In order to investigate the relationship between Graves’ disease, its associated ophthalmopathy and smoking, we followed retrospectively a group of 270 patients with Graves’ disease (233 females and 37 males). Smoking incidence within this group was compared to that found in a control, thyroid disease-free group of 120 patients. The incidence of smokers was significantly higher in the group with Graves’ disease (145 out of 270, 54%) when compared to the control ...

ea0014p302 | (1) | ECE2007

Fewer and fewer thyroidectomies in the treatment of Graves’ disease

Radu Diaconescu Mihai , Glod Mihai , Costea Ioan

Background: Surgery which was until sixty years ago the only treatment available for Graves’ disease (Gd) is now the last recommended therapeutical option, the number of thyroidectomies (Tx) being in continous decrease. American physicians prefere radioiodine use while their European and Japanese colleagues like better long-term administration of antithyroid drugs.Patients and methods: A retrospective study carried out on 52 consecutive patients wit...

ea0014p303 | (1) | ECE2007

The prevalence of thyroid cancer in Albania

Ylli Agron , Toti Florian , Furreraj Thanas , Husi Gerond , Sinjari Evis , Shehu Medi , Celiku Edmond

Introduction: The prevalence of thyroid cancer is increasing worldwide as well as in Albania. For the first time we have created the National Thyroid Cancer Register, including the period 2000–2005.Aim: Define the prevalence of thyroid cancer in Albanian population, the prevalence of different histopathologic forms of cancer and the probable risk factors.Results: During this period 83 patients were diagnosed of Thyroid cancer....

ea0014p304 | (1) | ECE2007

The prevalence of anti-C1q antibodies is increased in autoimmune thyroid disorders

Potlukova Eliska , Jiskra Jan , Sandova Petra , Limanova Zdenka , Smutek Daniel , Mareckova Helena , Antosova Marie , Trendelenburg Marten

Background: Autoantibodies directed against complement C1q (anti-C1q) have been described in a number of systemic autoimmune disorders. In systemic lupus erythematosus, they are strongly associated with proliferative lupus nephritis. However, no study has focused on the presence of anti-C1q in organ specific autoimmune disorders. The aim of this study was to determine the prevalence of anti-C1q in autoimmune thyroid disorders (AITD).Methods: Serum levels...

ea0014p305 | (1) | ECE2007

The analgesic efficacy of lidocaine/prilocaine (EMLA) cream during the fine-needle aspiration biopsy of thyroid nodules

Gursoy Alptekin , Ertugrul Derun , Sahin Mustafa , Bascil Tutuncu Neslihan , Nar Demirer Asli , Guvener Demirag Nilgun

Pain is one of the few drawbacks of fine-needle aspiration biopsy (FNAB) in patients with nodular thyroid disease (NTD). Lidocaine/prilocaine cream, an eutectic mixture of local anesthetics (EMLA), is a frequently used topical anesthetic. Despite its well-documented efficacy for the relief of pain associated with other cutaneous procedures that involve needle insertion, the analgesic role of EMLA has not been previously reported in patients with NTD who are undergoing FNAB. Th...

ea0014p306 | (1) | ECE2007

Radioactive iodine in the treatment of type 2 amiodarone-induced thyrotoxicosis

Gursoy Alptekin , Bascil Tutuncu Neslihan , Gencoglu Arzu , Yilmaz Hamiyet , Nar Demirer Asli , Guvener Demirag Nilgun

Objective: Amiodarone-induced thyrotoxicosis (AIT) is usually classified into 2 types (type 1, in which a high iodine content triggers the autonomous production of thyroid hormone; and type 2, in which destructive thyroiditis causes the release of preformed thyroid hormone). A mixed form of AIT has also been also described. AIT is a difficult management problem that sometimes requires ablative thyroid therapy. The use of radioactive iodine (RAI) therapy in patients with type 1...

ea0014p307 | (1) | ECE2007

The prevalence of hyperprolactinaemia in overt and subclinical hypothyroidism

Hekimsoy Zeliha , Kafesçiler Sabriye , Güçlü Feyzullah , Özmen Bilgin

Hyperprolactinaemia can occur in patients with primary hypothyroidism. Although its prevalence in overt hypothyroidism varies from 0 to 40%, its prevalence and clinical significance in subclinical hypothyroidism has not been studied. In this prospective, observational study, serum levels of prolactin (PRL) were measured in 167 consecutive patients presenting to our endocrinology clinic for evaluation of hypothyroidism, and correlation of PRL levels with the severity of hypothy...

ea0014p308 | (1) | ECE2007

The role of parathyroid hormone monitoring after total thyroidectomy in predicting post-thyroidectomy related hypocalcaemia

Athanassiou Evangelos , Christodoulidis Gregory , Hatzitheofilou Constantinos , Tepetes Constantinos , Zacharoulis Demetrios , Tzovaras George , Euthymiou Manthos

Objective: Clinically apparent hypocalcaemia following total thyroidectomy occurs in 20–25% of patients subjected to total thyroidectomy, in 2-4% of these patients the hypocalcaemia is permanent. Treatment by per-os or iv administration of calcium supplements and vitamin D is suggested when the serum calcium concentration falls bellow a critical level, either before or whenever the patients develop clinical symptoms of tetany. The purpose of this study is to evaluate the ...

ea0014p309 | (1) | ECE2007

Abstract unavailable...

ea0014p310 | (1) | ECE2007

Intraorbital tissues effects of rituximab (RTX) treatment in patients with thyroid-associated ophthalmopathy (TAO)

Vannucchi Guia , Campi Irene , Rossi Stefania , Bonara Paola , Guastella Claudio , Currò Nicola , Simonetta Simona , Sina Clara , Ratiglia Roberto , Beck-Peccoz Paolo , Salvi Mario

We previously described a significant response to RTX treatment in patients with active TAO, with no effect on TRAb and hyperthyroidism. In order to study the effect of RTX in the orbit, we analyzed the orbital tissues of 9 patients with TAO at decompression after RTX (n.2) or other treatments. Decompression was carried out in 2 patients for sight threatening optic neuropathy and in 7 for correction of proptosis. Of the RTX treated patients, one was decompressed after 12 month...

ea0014p311 | (1) | ECE2007

Thyroid and gastric autoimmune diseases

Morel Stéphanie , Georges Agnès , Bordenave Laurence , Corcuff Jean-Benoît

Background & aim: Autoimmune thyroid disease (AITD) is frequently accompanied by other organ-specific diseases. The aim of this study was to estimate the frequency of the association AITD-Biermer’s disease (pernicious anemia) by investigating the presence of intrinsic factor antibodies (IF-Ab) in the serum of patients with AITD.Methods: Sera from patients with biological signs of AITD (increased serum TSH levels associated to detectable thyroid ...

ea0014p312 | (1) | ECE2007

VEGF, FGF and HGF in differentiated thyroid cancer

Przybylik-Mazurek Elwira , Huszno Bohdan

Pathogenesis of thyroid cancer involves a number of biological, and environmental factors. The growth factors have mitogenic, proliferative and dedifferentiating effects. Some of the cytokines: Vascular Endothelial Growth Factor (VEGF), Fibroblast Growth Factor (FGF), Hepatocyte Growth Factor (HGF) are detected in a neoplasmatic tissue. Moreover, there are affected thyroid cancer cell growth and function in vitro.Aim of the study: The aim of the s...

ea0014p313 | (1) | ECE2007

Fas and FasL expression on peripheral lymphocytes in patients with autoimmune thyroid disease

Fountoulakis Stelios , Vartholomatis George , Philippou George , Tsatsoulis Agathocles

Objective: The Fas/Fas ligand (FasL) apoptotic pathway is activated in patients with autoimmune thyroid disease (AITD). It is believed that Fas and FasL expression in intrathyroidal T lymphocytes and thyrocytes is regulated in a manner resulting in thyroid cell apoptosis in Hashimoto’s thyroiditis (HT) or lymphocyte apoptosis in Graves’ disease (GD). The hypothesis that Fas and FasL may be differentially expressed on peripheral lymphocytes in patients with HT and GD ...

ea0014p314 | (1) | ECE2007

A novel pro-migratory action of TGFb in papillary carcinoma

Rodríguez García-Renueles María Elena , Bravo López Susan Belen , Seoane Rafael , Puig Carmen Gil , Teijeiro José Cameselle , Barreiro Francisco , Villamarín Clara Álvarez

Neoplastic thyroid diseases (multinodular goiter (MNG), follicular adenoma, differentiated (DTC) and undifferentiated thyroid carcinoma) have a higher incidence in women than in men. In fact, in the last ten years, DTC is the only cancer increasing the frequency in women, with an incidence similar to ovarian carcinoma or lymphomas.TGFb is a secreted factor important in the normal function of the thyrocyte. It has two independent actions: a fast antiproli...

ea0014p315 | (1) | ECE2007

Significance of accurate serum thyroglobulin cut-off values in the postoperative follow-up of differentiated thyroid carcinoma

Boér András , Vincze Borbála , Sinkovics István , Keresztes Sándor , Kapuvári Bence , Boldizsár Mariann , Remenár Éva , Otto Szabolcs

The present study was undertaken to evaluate the diagnostic performance of a high-sensitive thyroglobulin (Tg) immunoradiometric assay (BRAHMS Tg-S) in the follow-up of papillary and follicular thyroid cancer patients treated with total/nearly total thyreoidectomy and radioiodine ablation therapy. During TSH suppression serum Tg concentration was measured 6 weeks prior to the radioiodine ablation (onT4-Tg before ablation) as well as 3 months following treatment (onT4–Tg a...

ea0014p316 | (1) | ECE2007

Thyroid ultrasonography and ultrasonography-guided fine-needle aspiration biopsy of thyroid nodules in correlation with pathological findings

Poplawska-Kita Anna , Dzieciol Janusz , Lebkowska Urszula , Zonenberg Anna , Kita Arkadiusz , Siewko Katarzyna , Górska Maria

Introduction: Ultrasonography-guided fine-needle aspiration biopsy (ug-FNAB) is recommended as the first and most important step in the management of nodular thyroid disease.Material and methods: We compared the results of ultrasonography examination (US) and the ug-FNAB of the thyroid gland with postoperative histpathological findings in 387 patients with thyroidectomy operated on (61 cytological and 326 clinical indication).Resul...

ea0014p317 | (1) | ECE2007

Different prevalence of type 1 and type 2 amiodarone-induced thyrotoxicosis over a 30-year period

Tomisti Luca , Dell’Unto Enrica , Cosci Chiara , Sardella Chiara , Bartalena Luigi , Bogazzi Fausto , Martino Enio

Amiodarone induced thyrotoxicosis (AIT) may develop in patients with either underlying thyroid disorders (type 1) or normal gland (type 2). The latter is considered a drug-induced destructive thyroditis, usually responding to glucocorticoids. Further treatments after restoring euthyroidism are often not necessary. The former is a true form of iodine-induced hyperthyroidism the management of which includes thionamides, potassium perchlorate and thyrodectomy. The prevalence of t...

ea0014p318 | (1) | ECE2007

Carbamazepine and risk of hypothyroidism: a prospective study

Horacek Jiri , Simko Julius , Waberzinek Gerhard

While carbamazepine (CBZ) treatment may affect serum thyroid hormone concentrations it rarely leads to clinically important hypothyroidism. This study was aimed to evaluate an early effect of CBZ on thyroid status in hypothyroid patients with thyroid hormone replacement, as compared with patients without a thyroid disorder.Twenty-nine patients indicated for CBZ treatment were followed prospectively. Their thyrotropin (TSH), total thyroxine (TT4) and free...

ea0014p319 | (1) | ECE2007

Epidemiology of thyroid cancer in the north eastern region of Poland

Zonenberg Anna , Telejko Beata , Nikolajuk Agnieszka , Rybaczuk Mikolaj , Dzieciol Janusz , Zimnoch Lech , Kokoszko Maciej , Dadan Jacek , Wasielica Mieczyslaw , Borawski Jacek , Puchalski Zbigniew , Juczewska Marzena , Kinalska Ida , Gorska Maria

Before the introduction of mandatory salt iodination in 1997 the North-Eastern Region of Poland was known to be a moderate iodine deficiency area. Moreover, it was one of the mostly contaminated regions after the Chernobyl accident in 1986. The aim of our study was to evaluate the descriptive epidemiological features of incident thyroid cancers diagnosed among the residents of this area between 1996 and 2005. The Regional Cancer Surveillance Program was used to collect data on...

ea0014p320 | (1) | ECE2007

The Na+/I symporter (NIS) transports two of its substrates, I and ClO4, with different stoichiometries

Dohan Orsolya , Portulano Carla , Basquin Cecile , Amzel L Mario , Carrasco Nancy

The sodium/iodide transporter (NIS) mediates active I− uptake in thyroid, lactating breast, salivary gland, and stomach epithelial cells. NIS-mediated I− transport is electrogenic with a 2:1 Na+:I− stoichiometry, i.e. when NIS activity is measured electrophysiologically in NIS-expressing oocytes, inward positive currents are recorded upon addition of I− or other anions that are NIS substrates. Howev...

ea0014p321 | (1) | ECE2007

Estimation of influence radioiodine treatment on course of Graves’ ophthalmopathy

Nechaeva Olga , Dreval Alexander , Shestakova Tatiyana , Bardeeva Yulia , Komerdous Irina , Perepelova Oksana , Chzikh Irina

Aim: To estimate influence of radioiodine treatment of Graves’ disease (GD) on course of Graves’ ophthalmopathy (GO).Material and methods: 9 patients with GD and mild or moderate GO were included to the study (3 (33%) men and 6 (67%) women); the anamnesis of smoking had 4 persons (44%). Diagnosis of GO was evaluated by determination of severity and activity of disease with CAS, presents of diplopia, orbital ultrasound. CAS before radioiodine tr...

ea0014p322 | (1) | ECE2007

Influence of a subclinical thyrotoxicosis on heart in various age-grades

Babenko Alina , Grineva Elena , Vashrameeva Natalia

Subclinical thyrotoxicosis (ST) characterized by low serum TSH and normal FT4 and FT3 concentrations. ST may cause changes of geometry of heart and developments of diastolic dysfunction. Influence of ST on this evolutions depending on age of patients, duration of S?, effect of TSH level is not clear. In present research the effects of ST on changes of EchoCG at a different age were studied. The present study includes 66 normotensive patients with ST witho...

ea0014p323 | (1) | ECE2007

Thyroid abnormalities during treatment with PEGIFNα-2a and PEGIFNα-2b in patients affected by HCV-related chronic disease: &br;a prospective randomized study

Scavuzzo Francesco , Monda Guido Di , Serino Domenico , Tartaglione Maria Teresa , Lampasi Filippo , Ascione Antonio , Volpe Raffaele

Background: Correlation between therapy with interferons, thyroid autoimmunity and dysfunction is widely reported. Currently we used two different pegilated-interferons (α-2a, α-2b).Aim: Evaluate a probable different behaviour of two PEG-IFN responsible of thyroid abnormalities.Patients and methods: We observed 236 consecutive naïve-patients with HCV-related chronic-disease undergoing a treatment with antiviral thera...

ea0014p324 | (1) | ECE2007

The effect of L-thyroxin theraphy on left ventricular diastolic dysfunction in patients with subclinical hypothyroidism

Saler Tayyibe , Saglam Zuhal Aydan , Korkmaz Kazim , Ataoglu Hayriye Esra , Yenigun Mustafa , Oguzhan Berrin

Aim: Subclinical Hypothyroidism (SH) is associated with cardiovascular disorders which may include increased risk for atherosclerosis, endothelial dysfunction and myocardial dysfunction.To investigate the prevalence of left ventricular systolic and diastolic dysfunction in patients with subclinical hypothyroidism and the effect of L-thyroxin therapy on myocardial performance using conventional echocardiographic parameters.Method: The study includes 95 pa...

ea0014p325 | (1) | ECE2007

Risk factors for thyrotoxic cardiomyopathy

Shulgina Veronika , Melnichenko Galina , Fadeev Valentine , Shornikov Sergey

Objective of the study: To examine risk factors for thyrotoxic cardiomyopathy (ThC).Methods: In retrospective study (1975 to 2003) 272 patients aged 54 [43; 62] years with different forms of toxic goiter in combination with cardiac rhythm disturbances with or without heart failure (HF) were included. Atrial fibrillation (AF) and/or atrial flutter and/or ventricular premature beats accompanied with HF were diagnosed in 80.5% (219/272) patients (group 1), ...

ea0014p326 | (1) | ECE2007

Partial withdrawal of levothyroxine to stimulate serum thyroglobulin (TG) in the follow-up of differentiated thyroid carcinoma (DTC)

Ianni Francesca , Rota Carlo Antonio , Corsello Salvatore Maria , Rosa Annapina De , Gallo Francesca , Paragliola Rosa Maria , Ingraudo Francesca , Ricciato Maria Pia , Maussier Maria Ludovica , Salvatori Massimo , Pontecorvi Alfredo

Aim: We compared effectiveness of partial withdrawal of levothyroxine (L-T4) to the use of recombinant human TSH (rhTSH) in preparation for Tg testing. We also evaluated clinical aspects and quality-of-life (QOL) during both regimens.Materials and methods: Ten consecutive patients, previously treated with total thyroidectomy and radioiodine ablation for DTC, underwent rhTSH protocol and, after 15 days, reduced their L-T4 dose by 50% for 5 weeks. At the f...

ea0014p327 | (1) | ECE2007

Selected markers of endothelial dysfunction in patients with subclinical and overt hyperthyroidism

Modzelewska Anna , Szelachowska Malgorzata , Zonenberg Anna , Gorska Maria

Objective: The aim of this study was to evaluate the influence of subclinical and overt hyperthyroidism on the chosen markers of endothelial function.Material and methods: The groups studied consisted of 97 hyperthyroid subjects (51 with subclinical and 46 with overt hyperthyroidism) and 39 healthy controls matched for age, gender and body mass index. The following parameters were measured: TSH, FT3, FT4 (by MEIA), VCAM-1 (vascular cell adhesion molecule...

ea0014p328 | (1) | ECE2007

The changes in the incidence of nodular goitre, thyroid cancer and urine excretion of iodine in the inhabitants of north eastern Poland in 1997 and 2005

Zonenberg Anna , Zarzycki Wieslaw , Telejko Beata , Modzelewska Anna , Nikolajuk Agnieszka , Rybaczuk Mikolaj , Dzieciol Janusz , Siewko Katarzyna , Kinalska Ida , Gorska Maria

A higher incidence of nodular goitre and less differentiated types of thyroid carcinoma have been observed in iodine deficient regions. North-Eastern Poland was an area with a moderate deficiency of iodine until the introduction of the mandatory salt iodination in 1997 (30±10 mg KJ/kg NaCl). The aim of our study was to compare the incidence of goitre, thyroid carcinoma and urine excretion of iodine in the inhabitants of the North-Eastern Region of Poland in 1997 and 2005....

ea0014p329 | (1) | ECE2007

The role of deiodinases in thyronamine biosynthesis

Piehl Susanne , Gabor Balizs , Thomas Heberer , Köhrle Josef

Deiodinases (5′-D1, 5′-D2, 5-D3) control the systemic and local bioavailability of thyroid hormones by removing iodine from their substrates. Thyronamine (T0AM) and 3-iodothyronamine (3-T1AM) are possible novel metabolites of classical thyroid hormones which have been demonstrated to occur endogenously and to display unique effects such as reducing body temperature in mice and activating the plasma membrane bound G-Protein coupled receptor TAAR1 (Scanlan et al.<...

ea0014p330 | (1) | ECE2007

Thyroid disease prevalance in Cushing’s disease

Toptas Tayfur , Tiryakioglu Ozay , Yetkin Demet Ozgil , Kadioglu Pinar

Purpose: To determine the prevalence of nodular thyroid disease, autoimmune thyroid disease, goiter and primary thyroid dysfunction in patients with active Cushing’s disease.Patients and methods: Nineteen patients with active Cushing’s disease (17 female, 2 male, mean age 43.16±3.55 years) and forty, age and gender matched healthy volunteers who served as the control group (34 female, 6 male, mean age 47.28±2.31 years) were included i...

ea0014p331 | (1) | ECE2007

From sampling to analytics: experience and diagnostic consequences with some thyroid markers

Toldy Erzsebet , Locsei Zoltan , Szabolcs Istvan , Toldy Erzsebet , Kovacs Gabor L.

In the clinical laboratory practice, endocrine biomolecules are mainly measured by immunoassay. Storage of the samples can not be avoided in many cases. Measurement in the low concentration ranges require exact knowledge on how storage would influence the functional sensitivity of the measurement.Aim: To evaluate the effect of storage of serum samples on their stability and the functional sensitivity of the applied method.Methods: ...

ea0014p332 | (1) | ECE2007

Gq/11-dependent signaling of the tyhrotropin receptor regulates metallothionein 1 expression in human thyroid carcinoma cells

Buech Thomas , Baeck Christer , Nowak Nicola , Gudermann Thomas

Metallothioneins (MT) are cystein-rich intracellular proteins which exert anti-apoptotic effects by protecting cells against oxidative stress and DNA damage. Previously, expression of MT in normal and neoplastic thyroid tissue has been demonstrated. However, the thyroidal regulation of MT expression is widely unsettled. Thus, we investigated the expression of MT isoform 1 in human thyroid carcinoma cells (FTC-133-TSHR) upon stimulation with thyrotropin (TSH). Using quantitativ...

ea0014p333 | (1) | ECE2007

Association of cytokine gene polymorphism with Graves’ disease in Turkish population

Kutluturk Faruk , Yarman Sema , Savran Fatma Oguz , Kekik Cigdem

Cytokines play a crucial role in the pathogenesis of autoimmune thyroid disease, and recent studies have demonstrated an association between cytokine gene polymorphisms and Graves’ disease (GD) in different ethnic groups.The aim of the present study was to investigate the relationship of IL-6, IL-10, TNF-α, TGF-β, and INF-γ gene polymorphisms with the development of GD in Turkish population. A total of 224 subjects were included in the study comprising of 1...

ea0014p334 | (1) | ECE2007

Hashimoto’s encephalitis: role of diagnostic SPECT

Martin Tomas , Gentil Alfonso , Torres Alberto , Maraver Silvia , Diaz-Galvez Milagrosa

In autoimmune thyroid disease some diverse neurological alterations like dementia, psychosis or peripheral neuropathy, are described. Hashimoto’s encephalopathy (EH) is a serious form of these neurological alterations. We describe three cases with different presentation and morphologic normal tests where cerebral SPECT was diagnostic.Case n°1. A 32-year-old male diagnosed of autoimmune hypothyroidism which presents paresthetics and muscular sti...

ea0014p335 | (1) | ECE2007

Usefulness of Ki-67, PCNA, c-erbB-2 and CK 19 in the diagnosis of some thyroid follicular tumors

Zosin Ioana , Cornianu Marioara , Golu Ioana , Balas Melania , Milos Aurora

This study represents a complex evaluation of a group of 26 cases with thyroid nodular disease (TND), using different diagnostic methods. Clinically and by means of ultrasonography, 12 patients were suspected of malignancy. Fine-needle aspiration biopsy (FNAB) detected mainly suspicious (12 cases) and malignant smears (5 cases). The microscopic examination of surgical specimens established the following diagnosis: follicular adenoma – FA (4 cases), papillary hyperplasia &...

ea0014p336 | (1) | ECE2007

Soluble CTLA-4 is increased in Graves’ disease and not related to thyroid status or ophthalmopathy severity

Daroszewski Jacek , Pawlak Edyta , Bolanowski Marek , Slowik Miroslaw , Frydecka Irena

Cytotoxic T-lymphocyte antigen-4 (CTLA-4) is a B7-binding protein that plays an important role in the down-regulation of T-cell activation. CTLA-4 function is closely associated with predisposition to autoimmune diseases. A native soluble form of CTLA-4 (sCTLA-4) is reported to be present in the sera of patients suffering from autoimmune thyroid disease. In this study we report data on sCTLA-4 concentrations in patients with clinical expression of Graves’ disease.<p c...

ea0014p337 | (1) | ECE2007

The relationship of epicardial fat thickness with carotid intima media thickness and endothelial function in subclinical and overt hypothyroidism

Yazici Dilek , Aydin Hasan , Özben Beste , Toprak Ahmet , Yavuz Dilek , Tarcin Ozlem , Sancak Seda , Deyneli Oguzhan , Akalin Sema

Hypothyroidism is associated with increased cardiovascular mortality. Epicardial fat thickness (EFT) has been found to be correlated with visceral fat accumulation and is thought to be a novel cardiovascular risk factor. The aim of this study was to determine EFT and its relationship with carotid intima media thickness (CIMT) and flow-mediated vasodilation (FMD) in subclinical and overt hypothyroid patients.Ten patients with overt (Group H) (42.2±15...

ea0014p338 | (1) | ECE2007

Prevalence of thyroid antibodies in gestational diabetes mellitus

Ruas Luísa , Paiva Sandra , Campos Maria , Marta Elvira , Lobo António , Cravo Annette , Carvalheiro Manuela

Background: Pregnancy alters the natural history of autoimmune thyroid disorders. The incidence rate of positive thyroid antibodies (T-abs +) in asymptomatic women during pregnancy has been reported to be between 6 and 19.6%.Aim: To determine the prevalence of thyroid antibodies (T-abs) in Gestational Diabetes Mellitus (GDM).Subjects & Method: In 408 women, at the time of diagnose of GDM, TSH, free thyroxine, free tri-iodothyro...

ea0014p339 | (1) | ECE2007

Thyroid investigation profile in patients with Hashimoto’s thyroiditis associated with other autoimmune disorders

Poiana Catalina , Peretianu Dan , Carsote Mara

Introduction: The prevalence of autoimmune disorders (AID) is more frequent in patients with Hashimoto’s thyroiditis (HT).Aim: To see if the clinical and biochemical aspects are different among the patients with HT and if they change related to the systemic or organ specific AID.Material and method: A. HT was diagnosed on antithyroperoxidase antibodies (ATPO) over 34 UI/ml. B. 491 patients with HT were investigat...

ea0014p340 | (1) | ECE2007

Ultrasound patterns in patients with autoimmune thyroiditis

Peretianu Dan , Carsote Mara , Poiana Catalina

Objectives: To analyze the conceptual frame for a correlation between thyroid echographic description and antithyroid peroxydase antibodies (ATPO) levels in Hashimoto’s thyroiditis (HT).Material-methods: A. 783 patients: 396 with HT (ATPO>34 UI/ml), 386-control (ATPO<34 UI/ml). B. Ultrasound aspects were described in 8 patterns: 0-thyroid lack; 1 – hypoechogenic and pseudonodular; 2 – hypoechogenic and homogenous; 3 &...

ea0014p341 | (1) | ECE2007

Iodine intake in Portugal: preliminary results in pregnant women

Limbert Edward , Prazeres Susana , Miranda Ana , Castro João , Carrilho Francisco , Reguengo Henrique , Borges Fatima

Introduction: Iodine is the key element for the synthesis of thyroid hormones and its intake modulates the physiology and physiopathology of thyroid gland. In Portugal, endemic goiter has disappeared, but some data make us consider that iodine intake, as in other European areas, is far from being sufficient. Taking into account the potential harmful effects of moderate iodine deficiency during pregnancy, when needs are increased, and the absence of recent data on iodine intake...

ea0014p342 | (1) | ECE2007

“False negative” results of Tg in patients with DTC (differentiated thyroid carcinoma)

Voliotopoulos Vassilios , Gianakopoulos Vassilios , Valais Ioannis , Charalabidou Isaia

Purpose: The long-term monitoring of patients with differentiated thyroid carcinoma is essential throughout the patient’s life after total or near total thyroidectomy followed by I-131 ablation after thyroid suppression using recombinant TSH (Thyrogen).As known the administration of rhTSH increase the sensitivity of Tg concentrations measurements. Antithyroglobulin antibodies are common clinical problem in patients with differentiated thyroid carcin...

ea0014p343 | (1) | ECE2007

The incidence of Hashimoto’s thyroiditis in the differentiated thyroid carcinoma

Voliotopoulos Vassilios , Gianakopoulos Vassilios , Valais Ioannis

Purpose: Hashimoto’s thyroiditis is medical disease witch affects more than 5% of the population and represents the most common cause of hypothyroidism. The possibility of an immunological and autoimmune mechanism in the pathogenia of the disease has been suggested.Methods and Materials: In 200 patients, who received iodine 131 therapy after total or near total thyroidectomy for one or more cold nodules,in our department last year (71% with papillar...

ea0014p344 | (1) | ECE2007

Thyroid cancer radioidine therapy using recombinant human TSH

Voliotopoulos Vassilios , Gianakopoulos Vassilios , Valais Ioannis

Purpose: The use of recombinant TSH (Thyrogen) has already entered in the clinical routine in order to avoid the discomfort and the morbidity associated with the withdraw of the thyroid hormone.Methods and Materials: We used the recombinant human TSH in 20 patients (age>50 years) totally or near totally thyroidectomized who came in our clinic to receive radioiodine therapy for locally invasive differentiated thyroid cancer. All patients were treated,...

ea0014p345 | (1) | ECE2007

Characterization of facilitative glucose transporters (GLUT) in human thyroid carcinoma cell lines

Ciampi Raffaele , Vivaldi Agnese , Piampiani Pamela , Agate Laura , Salvadori Piero , Del Guerra Alberto , Pinchera Aldo , Elisei Rossella

18FDG-PET is based on the capability of tumor cells to take up glucose. An increment in expression of the glucose transporter 1 (GLUT1) has been observed in thyroid tumors with poor prognosis but very few data are available about the expression of other glucose transporters in thyroid. Here, we study the expression and function of GLUT isoforms 1, 2, 3, 4, 6, 8, 10, 12 in human thyroid carcinoma cell lines ARO and FRO (anaplastic carcinoma), NPA (poorly-differentiated papillar...

ea0014p346 | (1) | ECE2007

Genotype/phenotype relation for toxic thyroid nodules with or without TSH receptor mutations

Gozu Hulya , Mueller Sandra , Bircan Rifat , Krohn Knut , Sargin Mehmet , Sargin Haluk , Gezen Cem , Erginer Turgay , Karadayi Nimet , Paschke Ralf

Constitutive activation of the cAMP pathway by activating TSHR mutation stimulates both thyrocyte proliferation and function. Thus they lead to formation of toxic thyroid nodules (TTNs) and ultimately hyperthyroidism. The in vitro activity of the various TSH-receptor mutation varies from 2–7 fold cAMP increase over the wild type TSH receptor. One previous study invrestigated a possible genotype to phenotype relation in TTNs with somatic TSHR mutation with a negativ...

ea0014p347 | (1) | ECE2007

High prevalence of ER22/23EK polymorphism of the glucocorticoid receptor gene in patients with Graves’ orbitopathy

Boyle Belema , Korányi Katalin , Bertalan Rita , Rácz Károly , Balázs Csaba

Objective: To investigate whether three polymorphisms of the glucocorticoid receptor gene known to influence the sensitivity to glucocorticoids could be implicated in the pathomechanism of Graves’ orbitopathy.Methods: Allelic frequencies of the ER22/23EK, Bcl l and N363S polymorphisms of the glucocorticoid receptor gene were investigated in 99 patients with Graves’ orbitopathy (mean age, 47.8±13.4 years) and in 175 healthy individuals (mea...

ea0014p348 | (1) | ECE2007

Removal of tick box for TFT in pathology request forms reduces TFT performed during acute medical admission

Piya Milan , Belbase Biparna , Thapa Meenakshi , Koppada Anu , Myers Martin , Baxter Susan , Rajbhandari Satyan

Joint UK guideline (2006) recommends that routine testing of thyroid function (TFT) in patients admitted acutely to hospital is not warranted unless specific clinical indications exist. Despite this, TFT is frequently requested during acute medical admission. In our previous audit in 2002, during a 1 month period from 18th September, 458 subjects were admitted to medical assessment unit (MAU) and 183 (40%) were offered TFT. 39 (29%) results were beyond the laboratory reference...

ea0014p349 | (1) | ECE2007

Thyroid hormones in serum and cerebrospinal fluid in patients with brain tumor and acute stroke

Katalinic Darko , Matovinovic Martina , Solter Miljenko , Maric Andreja , Murgic Jure , Vrkljan Milan , Dobrovic Karolina

We studied levels of T3, T4, FT3, FT4, rT3 and TSH concentrations in serum and FT3, FT4, rT3 and TSH concentrations in cerebrospinal fluid (CSF) in 10 patients with brain tumor and 20 patients with acute stroke and compared them to 7 patients in control group (further clinical evaluation in control group did not show brain lesions). All patients were euthyroid. The study was approved by loc...

ea0014p350 | (1) | ECE2007

The influence of universal salt-iodization on the iodine status of County Mures, detected through TSH determinations in newborns between 2001–2006

Szanto Zsuzsanna , Kun Ildiko , Kun I. , Coros Ligia , Cucerea Manuela

Iodine deficiency in a geographical area can be quantified not only by urinary iodine excretion, but by the frequency of elevated TSH-levels in newborns, too. We compared the TSH-levels obtained between 2001–2003 with those collected after extension of universal salt-iodization with increased iodine-content (KIO3 34±8.5 mg-/kg) in the whole country (2004–2006). The governmental decision was adopted in 2002, implemented in practice in December 2003, an...

ea0014p351 | (1) | ECE2007

The evolution of hypothyroidism in pregnant women in County Mures between 2001–2006

Kun Ildiko , Szanto Zsuzsanna , Kun I , Szabo B , Coros Ligia

County Mures is a moderate/mild iodine-deficient area, the iodine-prophylaxis having an important role in prevention of IDD. Between 2001–2003 we made a partial screening at 320 pregnant women to detect thy-roid dysfunctions, and in 13% (43 cases) we observed hypo-thy-roidism, the majority being subclinical forms. The most frequent compli-ca-tions were threatened abortion or premature birth, and dys-gra-vidia. We found that even the subclinical hypothyroidism can cause se...

ea0014p352 | (1) | ECE2007

The use of perchlorates in the treatment of some special forms of hyperthyroidism (report of two cases)

Kun I , Szanto Zsuzsanna , Let Emilia

The perchlorates block the enzyme NIS, inhibiting iodine accumulation in the thyroid and favour the elimination of intrathyroidal iodine unused for hormone synthesis. Their therapeutical utilisation actually is limited due to the toxicity. In the literature there are different opinions regarding the adverse effects (nephrotic syndrome, irreversible aplastic anemia etc.), but several authors sustain that these appear only after high doses, and after the development of therapeut...

ea0014p353 | (1) | ECE2007

Iodine deficiency detected through urinary iodine excretion in school-children living in goiter prevalent regions of County Mures (2005–2006)

Kun I , Balazs J , Nasalean Anisie , Gliga Camelia , Detesan Gabriela , Simescu Mihaela , Coros Ligia , Ionescu Anca , Madaras Gabriela , Szanto Zsuzsanna , Macarie C

Our previous studies made between 1999–2003 demonstrated that County Mures is a moderate/mild iodine-deficient geogra-phical area. In 2002 a governmental decision was given for universal iodization of alimentary salt with increased iodine-content, realized during 2004. The aim of our study was to investigate the effect of in-creased iodine-supplementation at school children living in different iodine-deficient areas in County Mures, through urinary iodine excretion (UIE)....

ea0014p354 | (1) | ECE2007

Endothelial function and hemostasis factors in hypothyroidism and subclinical hyperthyroidism

Gazdag Annamaria , Nagy V Endre , Varga Emma , Samson Laszlo , Erdei Annamaria , Jenei Zoltan

Long-term follow-up of differentiated thyroid cancer (DTC) patients requires yearly measurements of serum thyroglobulin (Tg) levels is endogenous (4 weeks off-thyroxin) or exogenous (human recombinant TSH injection) high TSH state. Subclinical hyperthyroidism is maintained in the patients throughout the rest of the year. We examined the endothelial function, hemostasis factors and metabolic parameters in short-term iatrogenic hypothyroidism (HO) and in subclinical hyperthyroid...

ea0014p355 | (1) | ECE2007

The relevance of thyroid echography in diagnostic of subclinical autoimmune thyroiditis during pregnancy and postpartum

Carmne Gorduza , Voichita Mogos , Vlad Gorduza Eusebiu , Eusebie Zbranca

Thyroid diseases are more frequent in women. We made a longitudinal study at 112 healthy pregnant women from Iasi county, Romania. The aim of study was to determine incidence and evolution of autoimmune thyroiditis in pregnancy and postpartum. The proceedings of study imposed to verify clinical aspect of thyroid, the volume of thyroid measured echographic, echostructure of thyroid, function of thyroid (TSH, FT4) and autoimmune modification of thyroid (anti-thyroid peroxidase a...

ea0014p356 | (1) | ECE2007

Outcomes of a fixed dose of 370 MBq of radioiodine in hyperthyroidism

Howat Isabel , Sawers Hilary , Sawers Stewart

In 1995 the Royal College of Physicians issued guidelines for the use of radioiodine in hyperthyroidism. They recommended administration of enough radioiodine to achieve euthyroidism, with acceptance of a moderate rate of hypothyroidism e.g. 15–20% at 2 years and 1–3% per annum thereafter. Guide activity was 400–550 MBq for standard hyperthyroidism (mainly Graves’ disease), at least 550 MBq for toxic multinodular goitre, and 300–500 MBq in toxic adenom...

ea0014p357 | (1) | ECE2007

The lymphocyte interactions in thyroid tissue in graves’ disease

Ben-Skowronek Iwona , Sierocinska-Sawa Jadwiga , Szewczyk Leszek , Korobowicz Elzbieta

Introduction: The T,B and antigen presenting cells play a key role in the pathogenesis of autoimmune diseases.The aim of the studies was to analyse different regulatory cells subsets interaction in patients with Graves’ disease.Material and methods: We have studied paraffin thyroid specimens obtained from 10 children with Graves’ disease after thiamazole treatment. The thyroid tissue was stained with hematoksylin-eosin (H...

ea0014p358 | (1) | ECE2007

Water purification technology reduces iodine content of drinking water and contributes to iodine deficiency

Samson Laszlo , Czegeny Ildiko , Nagy Endre V , Mezosi Emese , Erdei Annamaria

Drinking water is the major natural source of iodine in many European countries. In the present study, we examined possible sites of iodine loss during the usual water purification process. Water samples from 6 sites during the technological process were taken and analyzed for iodine content. Under laboratory circumstances, prepared iodine in water solution has been used as a model to test the effect of the presence of chlorine. Samples from the purification sites revealed tha...