Searchable abstracts of presentations at key conferences in endocrinology

ea0094p219 | Metabolism, Obesity and Diabetes | SFEBES2023

Broccoli extract as an agent to protect against endoplasmic reticulum stress and mitochondrial dysfunction in human adipocytes

Murphy Alice , Barker Guy , Piya Milan , Tripathi Gyanendra , McTernan Philip

Background: Endoplasmic reticulum (ER) stress and associated mitochondrial dysfunction contribute to the pathogenesis of obesity and type 2 diabetes mellitus (T2DM). Studies show broccoli can reduce inflammation in cancer and may therefore mitigate inflammation-induced cellular disruption that precedes insulin resistance in human adipocytes. Therefore these studies investigated the impact of freeze-dried broccoli extracts (BE) on ER stress and mitochondrial dy...

ea0059p168 | Obesity & metabolism | SFEBES2018

The impact of freeze dried broccoli extract to mitigate inflammation in human adipocytes through the mevalonate pathway

Murphy Alice , Azharian Sahar , Tripathi Gyanendra , Barker Guy , Chappell Michael , McTernan Philip G

Background: Delivery of nutrient excess in obesity can disrupt protein folding in the endoplasmic reticulum (ER) within adipose tissue; this activates the unfolded protein response (UPR) and contributes to type 2 diabetes mellitus (T2DM) risk. Thus, the aims of this study were to utilise freeze-dried broccoli extract (BE) as a nutrient to mitigate such cellular damage in human adipocytes, understand the relevance of associated pathways, and create a mathematical model of the U...

ea0044p191 | Obesity and Metabolism | SFEBES2016

Freeze dried broccoli extract relieves ER stress and mitochondrial inefficiency in differentiated human pre-adipocyte cells

Murphy Alice , de la Escalera Clapp Lucia Martinez , Jackisch Laura , Azharian Sahar , Aladel Alanoud , Barker Guy , Tripathi Gyanendra , Chappell Michael , McTernan Philip

Background: In obesity, excess nutrients can disrupt protein folding in the endoplasmic reticulum (ER) which activates the unfolded protein response (UPR) and alters mitochondrial function. These changes can induce inflammation, oxidative stress and insulin resistance. The aim of the study was to investigate whether broccoli extract can protect against cellular damage in human adipocytes, which with mathematical modelling may help predict pathway response.<p class="abstext...