Searchable abstracts of presentations at key conferences in endocrinology

ea0081ep692 | Pituitary and Neuroendocrinology | ECE2022

Soluble alpha klotho in blood is a new and highly stable biomarker

Ribeiro de Oliveira Longo Schweizer Junia , Haenelt Michael , Schilbach Katharina , Stormann Sylvere , Schopohl Jochen , Bidlingmaier Martin

Background: Soluble alpha klotho (sαKL) is a circulating protein that has been linked to the growth hormone (GH) axis. We previously showed its association to disease activity in patients with acromegaly, with considerable robustness towards biological confounders. However, there is scarce data in literature regarding the analytical performance of the assay, and pre-analytical stability of sαKL in blood samples. Objective: We aimed to evaluate ...

ea0049ep639 | Growth hormone IGF axis - basic | ECE2017

Modulation of the 22 kD/20 kD growth hormone (GH) ratio by exercise, fasting and glucose load in premenopausal women

Haenelt Michael , Schilbach Katharina , Gar Christina , Lechner Andreas , Schwaiger Rita , Bidlingmaier Martin

We previously have shown in a cohort of 236 non-fasted women that before and after a short high intensity exercise protocol (HIP) till exhaustion serum concentrations of 20 and 22 kD growth hormone (hGH) are highly correlated (P<0.0001). Although the GH response to exercise exhibited huge inter-individual differences, the 20 kD/22 kD-GH-ratio remained unchanged (9.6% vs 11.1%, P=0.313). A subgroup of the women was also studied under fasted conditions in a...

ea0081p660 | Pituitary and Neuroendocrinology | ECE2022

Soluble alpha klotho in adult patients with growth hormone deficiency

Ribeiro de Oliveira Longo Schweizer Junia , Schilbach Katharina , Haenelt Michael , Gagliardo Anica , Stormann Sylvere , Schopohl Jochen , Bidlingmaier Martin

Background: We recently have shown a close association of high concentrations of soluble alpha klotho (sαKL) to disease activity in acromegaly. Small pilot studies suggested that sαKL concentrations might be reduced in GH deficiency (GHD) and increase after recombinant human GH (rhGH) therapy. Our aim was to evaluate the potential of sαKL as a biomarker in GHD.Methods: We evaluated sαKL in comparison to the classical biomarkers GH, IG...

ea0049gp196 | Pituitary &amp; endocrine Tumours | ECE2017

Diagnosis of acromegaly: Sex and BMI are the major determinants of growth hormone suppression during oral glucose tolerance test (OGTT)

Schilbach Katharina , Nicolay Shiva Sophia , Haenelt Michael , Lechner Andreas , Gar Christina , Schopohl Jochen , Stormann Sylvere , Schwaiger Rita , Bidlingmaier Martin

GH suppression during OGTT is the gold-standard test in diagnosis and monitoring of acromegaly. However, discrepancies between GH nadir and IGF-I have been described and have triggered search for factors modifying the extent of GH suppression. Cut-offs at 1.0 or 0.4 ng/ml are currently recommended with modern, highly sensitive GH assays. To establish assay specific GH nadir cutoffs for the 22kD GH specific IDS-iSYS assay, we examined 381 subjects (319 females, 62 males) with n...

ea0070aep723 | Pituitary and Neuroendocrinology | ECE2020

Soluble alpha klotho measurement: Comparison of measurements by different commercially available assays in healthy subjects and active acromegaly

Ribeiro de Oliveira Longo Schweizer Junia , Schilbach Katharina , Haenelt Michael , Störmann Sylvère , Schopohl Jochen , Bidlingmaier Martin

Background: Recent studies reported high soluble α-klotho concentrations (α-klotho) in active acromegaly, with normalization after successful therapy. We showed that α-klotho correlates to GH and even better to IGF-I. So far, most studies employed an immunoassay from Immuno-Biological Laboratories (IBL) for measurement of α-klotho, but other assays are available. Agreement between assays remains questionable since α-klotho exists in several isoforms: K...

ea0056gp10 | Acromegaly | ECE2018

Human growth hormone (GH) isoforms during oral glucose tolerance test in patients with acromegaly and in healthy subjects

Ulmer Esther , Schilbach Katharina , Haenelt Michael , Nicolay Shiva Sophia , Schwerdt Laura , Schweizer Junia Ribeiro de Oliveira Longo , Bartel Christopher , Schopohl Jochen , Strasburger Christian , Wu Zida , Bidlingmaier Martin

GH consists of various molecular isoforms. Most abundant is 22 kDa-GH (80–90% of total GH), followed by 20 kDa-GH (5–15% of total GH). The biological significance of 20 kDa-GH remains unclear, but its effects appear comparable to those of 22 kDa-GH. Acromegaly is characterized by chronic GH excess. Data on GH isoforms in acromegaly are scarce, but an increased 20 kDa-/22 kDa-GH-ratio (20k-ratio) has been described. Our aims were to compare the 20k-ratio in a larger c...