Searchable abstracts of presentations at key conferences in endocrinology

ea0004oc22 | Steroid hormone action | SFE2002

Agonist activated adrenocorticotropin receptor internalises via a clathrin-mediated G-protein coupled receptor kinase dependent mechanism

Baig A , Hunyady L , Clark A

The physiological effects of the pituitary hormone, adrenocorticotropic hormone (ACTH) on the adrenal are mediated by the melanocortin 2 receptor (MC2R), a G protein coupled receptor (GPCR) that signals via adenylate cyclase to elevate intracellular cyclic AMP (cAMP) levels. Therefore the expression and function of the receptor are likely to be the major determinants of the response to ACTH. Following repeated stimulation, the cAMP signal is diminished or desensitised. Desensi...

ea0029p296 | Cardiovascular Endocrinology and Lipid Metabolism | ICEECE2012

Allosteric effects between the protomers of AT1 angiotensin receptor homodimer: role of the conserved DRY motif

Szalai B. , Varnai P. , Hunyady L.

Introduction: G protein coupled receptor dimerization is proposed to have great impact on receptor signaling. Allosteric communication between the protomers of a dimer can alter ligand binding, receptor conformation and the interactions with different effector proteins. Although the concept of intradimeric interactions is widely accepted, the molecular mechanism behind them is not clearly understood. In this study we investigated the allosteric interactions within the type I a...

ea0029p1162 | Nuclear receptors and Signal transduction | ICEECE2012

Characterisation of the N321K mutation in the type-2 vasopressin receptor causing nephrogenic diabetes insipidus

Erdelyi L. , Balla A. , Toth M. , Hunyady L.

Background: Loss of function mutations of the type-2 vasopressin receptor (V2R) in kidney lead to nephrogenic diabetes insipidus (NDI). The function of mutant receptors can be rescued using ligands resulting sufficient amount of plasma membrane localized V2Rs in a conformation which are able to generate cAMP signal. We examined a previously described, not characterized mutation. In order to determine personalized therapy for the individual the properties of the mutant receptor...

ea0029p286 | Cardiovascular Endocrinology and Lipid Metabolism | ICEECE2012

The role of β-arrestin proteins in CB1 cannabinoid receptor endocytosis

Hunyady L. , Gyombolai P. , Boros E. , Turu G.

Introduction: β-Arrestins are cytosolic proteins, which play key roles in the desensitization, internalization and signaling of G protein-coupled receptors. Currently, little is known about the role of β-arrestins in the internalization of the CB1 cannabinoid receptor (CB1R). Therefore, the interaction between β-arrestins and CB1R, and its role in receptor internalization were studied.Materials and methods: The β-arrestin binding of C...

ea0014s3.1 | Signaling and regulation of G-protein-coupled hormone receptors | ECE2007

Trafficking and signaling of angiotensin receptors

Hunyady László , Karip Eszter , Turu Gábor , Szidonya László

The octapeptide hormone angiotensin II (Ang II) exerts its major biological effects via angiotensin AT1 receptors (AT1Rs). Signaling of AT1Rs is regulated by ß-arrestins, which bind to activated AT1Rs, uncouple them form G proteins, and initiate their internalization via clathrin-coated pits and cause G protein independent MAP kinase activation. It has been shown previously that AT1Rs internalize via ß-arrestin-d...

ea0070aep7 | Adrenal and Cardiovascular Endocrinology | ECE2020

Investigation of angiotensin II induced gene expression changes in vascular smooth muscle cells

Balla András , Szalai Laura , Gém Janka , Kovács Kinga , Szakadáti Gyöngyi , Hunyady László

The angiotensin II (AngII) hormone exerts a number of biological effects through the type 1 angiotensin II receptor (AT1R). One of the main targets of AngII are vascular smooth muscle cells, their stimulation activates many signaling pathways and results in gene expression changes.Affymetrix Gene Chip experiments were performed to analyze the effects of AngII stimulation on gene expression. For the determinations, aortic thoracic aorta from young male Wi...

ea0014p163 | (1) | ECE2007

High prevalence of novel mutations of the MEN1 gene in Hungarian patients with multiple endocrine neoplasia type 1

Balogh Katalin , Hunyady László , Patócs Attila , Gergics Peter , Valkusz Zsuzsa , Tóth Miklós , Varga Ibolya , Gláz Edit , Rácz Károly

Introduction: Multiple endocrine neoplasia type 1 (MEN 1) may present as a familial or a sporadic disorder with multiple endocrine tumours including parathyroid adenomas or hyperplasias, tumours of endocrine pancreatic and pituitary gland. Familial and sporadic MEN 1-related states which do not fulfill current diagnostic criteria but may be related to MEN 1 syndrome have been also described.Aims: The aim of this study was to examine the prevalence and sp...