Searchable abstracts of presentations at key conferences in endocrinology

ea0025p292 | Steroids | SFEBES2011

Glycyrrhetinic acid disrupts the synthesis of adrenocorticosteroid hormones at multiple sites

Al-Dujaili Emad , Kenyon Christopher , Nicol Moira , Mason Ian

Background and aims: Previously we showed that the bioactive liquorice constituent glycyrrhetinic acid (GA) inhibits not only 11β-hydroxysteroid dehydrogenase enzymes but also the sulphation of corticosteroids by the enzyme sulphotransferase 2A1 (SULT2A1). Since pregnenolone, 17α-hydroxy-pregnenolone, deoxycorticosterone (DOC) and dehydroepiandrosterone (DHEA) are all prime substrates for SULT2A1, GA could have important effects in directing pathways of glucocorticoi...

ea0049gp4 | Adrenal 1 | ECE2017

Androgen receptor signalling is essential for regression of the adrenal x-zone and regulation of the adrenal cortex in the male mouse

Gannon Anne-Louise , O'Hara Laura , Mitchell Rod , Mason Ian , Smith Lee

Introduction: Androgens have long been known to play an important role in health and wellbeing. A range of clinical disorders in males and females can arise due to disruption to production and action of androgens. Androgen receptor (AR) is widely expressed throughout the adrenal cortex, yet the wider role for androgen signalling in the adrenal remains underexplored due to the lack of suitable animal models.Methods: An adrenal-specific androgen receptor k...

ea0021p216 | Endocrine tumours and neoplasia | SFEBES2009

Expression and regulation of oestrogen sulfotransferase (EST) in human ovarian surface epithelium (OSE) and epithelial ovarian cancer (EOC)

Ren Xia , Harlow Chris , Fegan Scott , Mason Ian , Critchley Hilary , Hillier Stephen

Ovarian cancer is the most fatal of all gynaecological malignancies. EOC accounts for >90% of malignant ovarian tumours and is thought to originate mostly from OSE cells. Epidemiological data suggest EOC is oestrogen responsive and we previously showed active oestrogen could be produced locally by EOC cells, but not by normal OSE cells, through the combined activities of steroid sulfatase (STS) and 17βhydroxysteroid dehydrogenase. We now investigate if EST, which conv...

ea0021p343 | Steroids | SFEBES2009

Application of a highly specific and sensitive ELISA for the estimation of cortisone in biological fluids

Baghdadi Hussam , Al-Dujaili Emad , Almoosawi Suzana , Howie Forbes , Mason Ian

It is now generally acknowledged that local tissue concentrations of cortisol and cortisone are modulated by site-specific actions of 11β-hydroxysteroid dehydrogenase (11β-HSD) isoenzymes 1 and 2. Cortisone, the inactive metabolite of cortisol is produced by type 2 11β-HSD. To assess 11β-HSD type 1 and 2 activity, the cortisone/cortisol ratio has to be accurately determined. Immunoassays to measure cortisone levels are not widely available and tend to lack ...