Searchable abstracts of presentations at key conferences in endocrinology

ea0056gp135 | Female Reproduction | ECE2018

Liquid chromatography-tandem mass spectrometry characterization of a broad serum and salivary steroid profile in classical form of polycystic ovary syndrome

Mezzullo Marco , Fanelli Flaminia , Zanotti Laura , Pagotto Uberto , Gambineri Alessandra

Polycystic ovary syndrome (PCOS) is a common endocrine disorder among premenopausal women affecting up to 20% of the population. Even though androgen excess is the main diagnostic criterion for PCOS, the evaluation of hyperandrogenemia relies on methods with low accuracy and mainly able to measure total androgen amounts. Liquid chromatography-tandem mass spectrometry (LC–MS/MS) allows high accuracy steroids measurement and if applied to saliva, it also allows the measurem...

ea0063gp142 | Interdisciplinary Endocrinology 1 | ECE2019

Impact of age and metabolic profile on the definition of 12 circulating steroid reference intervals measured by LC-MS/MS in an Italian healthy adult male cohort

Mezzullo Marco , Di Dalmazi Guido , Fazzini Alessia , Ibarra Gasparini Daniela , Pelusi Carlotta , Pagotto Uberto , Fanelli Flaminia

Background: Steroid profiling by LC-MS/MS is increasingly used in endocrine research, and is being introduced in the clinical practice. Due to the large disagreement with previous immunoassays, and to the enlarged panel of measurable steroids, novel and robust reference intervals (RIs) need to be established. Steroid circulating levels are known to be influenced by several physio-pathological factors, such as age, gender, stress and metabolic status. Therefore, generating unbi...

ea0063gp143 | Interdisciplinary Endocrinology 1 | ECE2019

Impact of age, menstrual phase, menopausal status and metabolic profile on the definition of 12 circulating steroid reference intervals measured by LC-MS/MS in an Italian healthy adult female cohort

Mezzullo Marco , Di Dalmazi Guido , Fazzini Alessia , Ibarra Gasparini Daniela , Gambineri Alessandra , Pagotto Uberto , Fanelli Flaminia

Endocrine clinical practice strongly relies on normative data for the assessment of hormonal imbalance. However, the generation of robust steroids reference intervals (RIs) remains a challenge. As the steroids circulating levels are influenced by many procedural aspects related to blood collection (time, fasting state) and physio-pathological factors, such as age, sex, stress and metabolic status, the need for partitioning RIs is mandatory. Furthermore, unbiased healthy, drug-...

ea0037gp.05.08 | Developmental and paediatric endocrinology | ECE2015

A simple and rapid method for steroid profiling by two dimensional liquid chromatography–tandem mass spectrometry: toward routine application

Fanelli Flaminia , Mezzullo Marco , Fazzini Alessia , Pedercini Matthew , Pasquali Renato , Pagotto Uberto

Steroid testing has a central role in clinical decision-making and in research studies on diseases such as hypercortisolisms, female hyperandrogenism, male hypogonadism or inborn disorders of steroid synthesis. Recently, liquid chromatography–tandem mass spectrometry (LC–MS/MS) proved its superiority to routine immunoassays (IA) in accurately and sensitively measuring low level steroids. However, the replacement of automated IA with LC–MS/MS platforms is limited...

ea0035p626 | Female reproduction | ECE2014

Simultaneous determination of salivary androgens by liquid chromatography–tandem mass spectrometry: analytical and biological validation

Mezzullo Marco , Fanelli Flaminia , Benetta Elisa Dalla , Garelli Silvia , Gambineri Alessandra , Pagotto Uberto , Pasquali Renato

The hormone assessment in saliva is emerging as a new valuable tool for clinical and research purposes. Salivary levels are supposed to reflect the free fraction of circulating steroids, thus representing a more informative indicator of their activity. Moreover, saliva collection is not stressful and does not require medical assistance, allowing reliable and multiple sampling in a day. Liquid chromatography–tandem mass spectrometry (LC–MS/MS) technology offers high s...

ea0035p757 | Obesity | ECE2014

Circulating endocannabinoids are associated with cardiometabolic impairment independently of adiposity in normal weight subjects

Fanelli Flaminia , Belluomo Ilaria , Cuomo Gaia , Mezzullo Marco , Baccini Margherita , Casadio Elena , Pasquali Renato , Pagotto Uberto

Obesity is characterized by hyperactivated endocannabinoid System (ECS) in brain and periphery and by increased levels of circulating endocannabinoids (cECs), anandamide (AEA) and 2-arachidonoylglycerol (2AG). It is still debated whether in absence of obesity cECs are only modulated by adiposity or if they are associated with cardiometabolic parameters.We recruited 155 healthy normal weight (NW, BMI: 18.5–24.9 kg/m2) drug-free volunteers ...

ea0056p889 | Adrenal cortex (to include Cushing's) | ECE2018

Are there specific biomarkers able to differentiate non classical congenital adrenal hyperplasia (NCAH) due to 21-hydroxylase deficiency from non-NCAH in a population of naive hyperandrogenic women in the reproductive age?

Oriolo Claudia , Menabo Soara , Baldazzi Lilia , Castelli Silvia , Pagotto Uberto , Mezzullo Marco , Fanelli Flaminia , Gambineri Alessandra

Objective: This study was aimed to evaluate the prevalence of non classical congenital adrenal hyperplasia due to 21-hydroxylase deficiency (21-NCAH) in hyperandrogenic women in the reproductive age attending our Endocrinology Unit and to identify specific phenotypic traits among clinical, biochemical and hormonal features.Setting: Outpatient Unit of Endocrinology, S. Orsola-Malpighi University Hospital of Bologna, Italy.Patients a...

ea0081ep860 | Reproductive and Developmental Endocrinology | ECE2022

A 20-steroid LC-MS/MS panel to investigate classical and backdoor pathways of androgen biosynthesis in the Leydig cells

Magagnoli Matteo , Mezzullo Marco , Limoncella Silvia , Tommasini Alessia , Santi Daniele , Pelusi Carla , Simoni Manuela , Pagotto Uberto , Casarini Livio , Fanelli Flaminia

Background: The classical steroidogenic route to the synthesis of dihydrotestosterone in Leydig cells involves delta-5 and delta-4 precursors. Besides, the “backdoor pathway”, encompassing progesterone metabolites, is gaining increasing interest in fetal development and men pathophysiology. Nowadays, liquid chromatography/tandem mass spectrometry (LC-MS/MS) is the ideal technique to simultaneously quantify large panels of steroids with elevated sensitivity and specif...

ea0081ep879 | Reproductive and Developmental Endocrinology | ECE2022

Daytime salivary androgen rhythm by LC-MS/MS in women affected by Polycystic Ovary Syndrome (PCOS) fulfilling the three Rotterdam diagnostic criteria

Fanelli Flaminia , Hawley James M , Mezzullo Marco , Fazzini Alessia , Zanotti Laura , Magagnoli Matteo , Pagotto Uberto , Keevil Brian , Gambineri Alessandra

Background: Excess testosterone in PCOS is accompanied by increased levels of other ovarian and adrenal androgens and precursors. Recently, 11-oxygenated adrenal androgens have been postulated as major components of the circulating androgen pool in PCOS. Obesity is strictly connected with androgen excess and with the derangement of hormone circadian rhythmicity. To date, it has not been clarified whether hyperandrogenism in PCOS, either complicated by obesity or not, is accomp...

ea0049ep669 | Obesity | ECE2017

Menopause is a major determinant of endocannabinoid 2-arachidonoylglycerol plasma level and of its relevance as biomarker of dyslipidemia and insulin resistance in lean women

Fanelli Flaminia , Mezzullo Marco , Baccini Margherita , Casadio Elena , Gasparini Daniela Ibarra , Vicennati Valentina , Pasquali Renato , Pagotto Uberto

Context: The endocannabinoid system (ECS) hypertonicity contributes to obesity development and maintenance. Excess circulating levels of ECS mediator 2-arachidonoylglycerol (2AG) were reported in obese humans, however, unstandardized experimental settings (i.e. analytical conditions, sample size, concurrent diseases/medications, statistics, gender, body mass index (BMI) and age), clouded its role in dysmetabolism and its usefulness as biomarker. The study aimed at describing 2...