Searchable abstracts of presentations at key conferences in endocrinology

ea0020s18.1 | Should adrenal venous sampling should be performed before adrenalectomy in primary aldosteronism? | ECE2009

Adrenal venous sampling should be performed before adrenalectomy in primary aldosteronism?

Mulatero Paolo

Primary aldosteronism (PA) is the most frequent form of endocrine hypertension. The identification of the subtype of PA is fundamental to distinguish between subtypes that benefit from surgery and subtypes that should be treated pharmacologically with mineralocorticoid receptor antagonists. CT scanning is the most sensitive imaging technique for the identification of adrenal nodules but lacks sensitivity and specificity. Also posture test and other clinical criteria of ‘h...

ea0032s22.3 | Improving diagnosis of primary aldosteronism | ECE2013

Adrenal vein sampling

Mulatero Paolo , Monticone Silvia , Buffolo Fabrizio , Veglio Franco

Primary aldosteronism is the most common form of secondary hypertension. The detection of primary aldosteronism is of particular importance, because it provides an opportunity for a targeted treatment (surgical for unilateral forms and medical with mineralocorticoid receptor antagonists for bilateral forms). PA diagnosis comprises screening, confirmation testing and subtype diagnosis.Guidelines recommend that all patients for whom the surgical treatment ...

ea0049ep37 | Adrenal cortex (to include Cushing's) | ECE2017

Visinin-Like Protein-1 in the regulation of aldosterone biosynthesis

Gomez-Sanchez Celso , Kuppusamy Maniselvan , Ishimwe Jeanne , Williams Tracy , Mulatero Paolo , Gomez-Sanchez Elise

Visinin-Like Protein-1 (Vsnl1) is a member of the EF-hand calcium sensor protein family that is expressed in the zona glomerulosa of the rat and human adrenal and upregulated in aldosterone-producing adenomas.We studied the expression pattern of Vsnl1 and co-localization with the CYP11B2 enzyme and the zona glomerulosa (ZG) marker Dlk1 (ZOG) using double and triple immunofluorescence in the adrenal of rats on a normal, high and low sodium diets. We also ...

ea0037gp.03.04 | Adrenal (2) | ECE2015

Prolactinoma and primary aldosteronism: is there a causal link?

Williams Tracy Ann , Dietz Anna , Beuschlein Felix , Mulatero Paolo , Reincke Martin

Introduction: We report the largest case series of concomitant prolactinoma and aldosterone-producing adenoma (APA), diagnosed in four patients from the German Conn Registry and in three patients at the Hypertension Unit, University of Torino. A disproportionate number (five out of seven) presented with macro-prolactinomas indicating that elevated prolactin (PRL) concentrations may play a role in APA formation. Further, increased expression of PRLR in the zona glomeru...

ea0070aep19 | Adrenal and Cardiovascular Endocrinology | ECE2020

Genes mediating cell growth in aldosterone-producing adenomas

Yang Yuhong , Tetti Martina , Mulatero Paolo , Schneider Holger , Reincke Martin , Ann Williams Tracy

Objective: Aldosterone-producing adenomas (APA) are a major cause of primary aldosteronism. Somatic mutations explain the excess aldosterone production in the majority of patients with APA with mutations in KCNJ5 encoding a potassium channel the most prevalent in most reported populations. Mechanisms driving cell proliferation are largely undefined.Design and method: Quantitative transcriptome analysis using RNA-seq was used to identify differen...

ea0020p7 | Adrenal | ECE2009

Use of diurnal rhythm in salivary aldosterone to discriminate between bilateral adrenal hyperplasia and aldosterone producing adenoma

Manolopoulou Jenny , Gerum Sabine , Mulatero Paolo , Spyroglou Ariadni , Reincke Martin , Bidlingmaier Martin

Differential diagnosis between bilateral adrenal hyperplasia (BAH) and aldosterone producing adenoma (APA) in aldosteronism remains challenging in many cases due to the high prevalence of incidentalomas during imaging techniques, the limited sensitivity of orthostatic testing and the technical difficulties of adrenal vein sampling (AVS).We investigated circadian variation in salivary aldosterone (SA) in patients with APA (n=22) and BAH (n=2...

ea0063gp104 | Adrenal and Neuroendocrine - Basic | ECE2019

Identification of genes mediating dysregulated cell growth in aldosterone-producing adenomas

Yang Yuhong , Tetti Martina , Jaquin Diana , Mulatero Paolo , Schneider Holger , Beuschlein Felix , Reincke Martin , Williams Tracy Ann

Background: Aldosterone-producing adenomas (APA) are a major cause of primary aldosteronism. Somatic mutations explain the excess aldosterone production in the majority of patients with APA with mutations in the potassium channel KCNJ5 the most prevalent. In contrast, mechanisms driving cell proliferation are largely unresolved.Objective: To identify genes that modulate cell growth in APAs.Methods: Quantitative transcripto...

ea0037ep370 | Diabetes (pathiophysiology & epitemiology) | ECE2015

Comparison between aldosterone and renin measurement by chemiluminescent immunoassay and RIA for the diagnosis of primary aldosteronism

Burrello Jacopo , Buffolo Fabrizio , Monticone Silvia , Williams Tracy Ann , Viola Andrea , Mengozzi Giulio , Veglio Franco , Mulatero Paolo

Objective: Primary aldosteronism (PA) is the most frequent cause of secondary hypertension. According to the Endocrine Society Guidelines, up to 50% of hypertensive patients should be screened for PA, using the aldosterone to renin (or plasma renin activity (PRA)) ratio (AARR and ARR respectively). The automated Diasorin LIAISON chemiluminescent immunoassay for renin and aldosterone measurement became available and in many laboratories is currently used instead of the classica...

ea0037ep66 | Adrenal cortex | ECE2015

Clinical significance of contralateral adrenal suppression during adrenal vein sampling in primary aldosteronism

Monticone Silvia , Satoh Fumitoshi , Viola Andrea , Fischer Eveline , Vonend Oliver , Bernini Giampaolo , Quinkler Marcus , Giacchetti Gilberta , Morimoto Ryo , Willemberg Holger , Maccario Mauro , Veglio Franco , Ito Sadayoshi , Reincke Martin , Mulatero Paolo

Objective: Adrenal vein sampling (AVS) is recognised by Endocrine Society guidelines as the only reliable mean to distinguish between aldosterone producing adenomas and bilateral adrenal hyperplasia, the two most common subtypes of primary aldosteronism (PA). However, AVS protocols are not standardised and vary between centres. The objective of the present study was to assess whether the presence or absence of contralateral adrenal (CL) suppression has an impact on the postope...

ea0094oc5.1 | Adrenal and Cardiovascular | SFEBES2023

Urine steroid metabolomics as a diagnostic tool in endocrine hypertension

Prete Alessandro , Abdi Lida , Suntornlohanakul Onnicha , Lang Katharina , Riancho Julien , Lazkani Aida , Larsen Casper K. , Gimenez-Roqueplo Anne-Paule , Pecori Alessio , Tetti Martina , Monticone Silvia , Muller Lisa M. , Adolf Christian , Timmers Henri J.L.M. , Hampson Stephanie , Eisenhofer Graeme , Ceccato Filippo , Beuschlein Felix , Kabat Marek , Bertherat Jerome , Dennedy Conall , Davies Eleanor , Deinum Jaap , Reincke Martin , Paolo Rossi Gian , Mulatero Paolo , Amar Laurence , Zennaro Maria-Christina , Sitch Alice J. , Tino Peter , Biehl Michael , Taylor Angela E. , Arlt Wiebke

Background: Hypertension affects more than 30% of the adult population worldwide and is a major cardiovascular risk factor. Identifying secondary causes of hypertension is key to offering targeted treatment and mitigating adverse health outcomes. We tested the performance of urine steroid metabolomics (USM), the computational analysis of 24-hour urine steroid metabolome data by machine learning, for diagnosing endocrine hypertension.<str...