Searchable abstracts of presentations at key conferences in endocrinology

ea0056p860 | Pituitary - Clinical | ECE2018

Mind the gap between cortisol levels measured with second generation assays and current diagnostic thresholds for the diagnosis of adrenal insufficiency: a single center experience

Grassi Giorgia , Morelli Valentina , Polledri Elisa , Fustinoni Silvia , Chiodini Iacopo , Ceriotti Ferruccio , D'Agostino Simona , Mantovani Giovanna , Arosio Maura

Objective: The current cut-offs to diagnose adrenal insufficiency (AI) have been established using outdated immunoassays. More modern methods have demonstrated less cross-reactivity with other steroids. The aim of our study was to evaluate the correlation between the cortisol assay Roche Cortisol I (R1), the newly available Roche Cortisol II (R2) and liquid chromatography tandem mass spectrometry (LC-MS/MS), the gold standard procedure for the measurement of steroids.<p cl...

ea0063p322 | Reproductive Endocrinology 1 | ECE2019

Assessment of biochemical hyperandrogenism in PCOs by liquid chromatography tandem mass spectrometry using a multisteroid kit: focus on testosterone and androstenedione

Grassi Giorgia , Morelli Valentina , Polledri Elisa , Fustinoni Silvia , Chiodini Iacopo , Ceriotti Ferruccio , D'Agostino Simona , Filippi Francesca , Somigliana Edgardo , Mantovani Giovanna , Arosio Maura

Objective: The identification of hyperandrogenism represents the cornerstone for the assessment of polycystic ovary syndrome (PCOs). However, its definition has always been troubling, mostly because of the poor accuracy shown by routine androgens assays. As suggested by literature, the application of more precise steroid measurement methods (such as liquid chromatography tandem mass spectrometry, LC-MS/MS) could improve the diagnostic workup. The aim of our study is to evaluat...

ea0049ep1043 | Pituitary - Clinical | ECE2017

Salivary and serum cortisol levels by liquid chromatography tandem mass spectrometry after standard dose ACTH test in the diagnosis of central hypopituitarism

Morelli Valentina , Polledri Elisa , Mercadante Rosa , Sonzogni Beatrice , Mantovani Giovanna , Palmieri Serena , Malchiodi Elena , Verrua Elisa , Barbieri Anna Maria , Ferrante Emanuele , Arosio Maura , Fustinoni Silvia , Chiodini Iacopo

Objective: The diagnosis of central hypoadrenalism (HPAI) is a major clinical challenge. The gold standard procedure remains insuline tolerance test (ITT). Liquid chromatography tandem mass spectrometry (LC-MS/MS) is considered the best procedure for the evaluation of cortisol levels. This study aimed to evaluate cut-offs of serum (TM-SeC) and salivary cortisol (SaC) by LC-MS/MS and serum cortisol by ECLIA assay (E-SeC) after Standard dose ACTH test (SDCT) in diagnosing HPAI.<...

ea0056p14 | Adrenal cortex (to include Cushing's) | ECE2018

Preoperative treatment with metyrapone in patient with Cushing’s syndrome due to adrenal adenoma: a pilot prospective study

Puglisi Soraya , Perotti Paola , Barbot Mattia , Cosio Paolo , Scaroni Carla , Stigliano Antonio , Lardo Pina , Morelli Valentina , Polledri Elisa , Chiodini Iacopo , Reimondo Giuseppe , Pia Anna , Terzolo Massimo

Background and objectives: Metyrapone has been approved for the treatment of patients with CushingÂ’s syndrome from all causes, but only few retrospective clinical studies are available. The aim of our study was the prospective assessment of metyrapone as pre-operative treatment.Methods: Before adrenalectomy, 7 patients with ACTH-independent CushingÂ’s syndrome due to adrenal adenoma were prospectively treated with metyrapone for 3 months, with e...

ea0063oc7.1 | Endocrine Connections 1 | ECE2019

Cortisol suppression or peripheral sensitivity and activation are associated with diabetes, hypertension and fragility fractures in postmenopausal eucortisolemic women

Aresta Carmen , Chiodini Iacopo , Gaudio Agostino , Eller-Vainicher Cristina , Morelli Valentina , Zhukouskaya Volha V , Merlotti Daniela , Orsi Emanuela , Barbieri Anna Maria , Fustinoni Silvia , Polledri Elisa , Gennari Luigi , Falchetti Alberto , Carnevale Vincenzo , Persani Luca , Scillitani Alfredo

Background: Cortisol excess is associated with a higher prevalence of hypertension (Hy), type 2 diabetes (T2D) and fragility fractures (FX). A possible association between T2D and fragility FX with the degree of glucocorticoid (GC) suppression and peripheral activation or sensitivity even in non-hypercortisolemic subjects has been previously suggested.Aim: To assess if the degree of GC suppression or peripheral sensitivity and activation are associated w...