Searchable abstracts of presentations at key conferences in endocrinology

ea0077p185 | Metabolism, Obesity and Diabetes | SFEBES2021

Measurements of skin temperature in lean and obese humans at thermoneutrality and following cold exposure

Choong Kwok T’ng , Ramage Lynne E , Kelman Alexandra , Semple Robert K , Stimson Roland H

Background: Infrared thermography (IRT) can assess human brown adipose tissue (BAT) activity non-invasively. However, it remains unclear if skin temperature is altered in obesity. We compared skin temperatures of lean and obese individuals following cold exposure and studied its relationship with energy expenditure (EE).Methods: 10 lean (age 28.3±2.1y, BMI 21.5±0.4kg/m2) and 10 obese (age 28.2±2.1y, BMI 36.2±1.3kg/m2</...

ea0086p205 | Metabolism, Obesity and Diabetes | SFEBES2022

UCP1 expression in human brown adipose tissue is inversely associated with cardiometabolic risk factors

Choong Kwok T'ng , E Ramage Lynne , Kelman Alexandra , Wakelin Sonia J , Stimson Roland H

Introduction: Brown adipose tissue (BAT) increases energy expenditure and is a potential therapeutic target for obesity and associated cardiometabolic disease. It is unclear whether human BAT activity is reduced in obesity, as BAT 18F-fluorodeoxyglucose uptake is reduced but BAT metabolic activity measured using11C-acetate PET is preserved. BAT thermogenesis relies on the presence of uncoupling protein 1 (UCP1), which uncouples oxidative phosphorylation f...

ea0021oc3.2 | Young Endocrinologists prize session | SFEBES2009

β-Cell specific overexpression of 11β-hydroxysteroid dehydrogenase type 1 reverses high fat diet-induced β-cell failure

Turban-Rajaonah Sophie , Liu Xiaoxia , Ramage Lynne , Mullins John J , Seckl Jonathan R , Morton Nicholas M

Insulin-resistant diabetes is characterised by progressive pancreatic β-cell dysfunction. Elevated pancreatic islet activity of the intracellular glucocorticoid amplifying enzyme 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) was hypothesized to drive this process in genetically obese rodents. To determine the direct effects of elevated 11β-HSD1 on β-cell function in diabetes in vivo we created a transgenic model overexpressing 11β-HSD1...

ea0021p172 | Diabetes and metabolism | SFEBES2009

Aldosterone production by the mouse adrenal gland is compromised by a high fat diet and 11β-hydroxysteroid dehydrogenase type 1 deficiency

Nilsson Frida , Morrison Hamish , Ramage Lynne , Morton Nicholas , Seckl Jonathan , Morley Steven , Kenyon Christopher

Deficiency of the intracellular glucocorticoid (GC) reactivating enzyme 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) invokes compensatory activation of the HPA axis. Nevertheless, 11β-HSD1−/− mice resist dietary-induced obesity indicating that target tissues are protected from GC actions. Since endocrine adaptation to diet is mediated by altered HPA activity, we investigated whether the adrenal response to high fat (HF) diet is compr...

ea0015oc8 | Young Endocrinologist prize session | SFEBES2008

β-cell 11β-Hydroxysteroid dehydrogenase type 1 contributes to type 2 diabetes

Turban Sophie , Ramage Lynne , Paterson Janice , Mullins John , Seckl Jonathan , Morton Nik

High systemic cortisol levels contribute to insulin resistance and may exacerbate the development of diabetes by impairing the insulin secretory response of the pancreatic beta-cells (β-cells). 11β-Hydroxysteroid dehydrogenase type 1 (11β-HSD1) is a key modulator of glucocorticoid effect within target tissues and high adipose or liver levels of this enzyme may contribute to obesity and/or metabolic disease. We investigated cellular localization of 11β-HSD1 ...

ea0094oc6.4 | Metabolism, Obesity and Diabetes | SFEBES2023

Human brown adipose tissue activity is regulated by the parasympathetic nervous system

Choong Kwok T'ng , Ramage Lynne E. , Gray Calum , Wakelin Sonia J. , JR van Beek Edwin , Kelman Alexandra , Semple Robert J. , Suchacki Karla J. , Stimson Roland H

Brown adipose tissue (BAT) is a therapeutic target for obesity and associated metabolic diseases, due to its role in non-shivering thermogenesis. BAT activation is mediated through sympathetic stimulation, but parasympathetic regulation of human BAT has not been demonstrated previously. We undertook RNA sequencing of human white and brown primary adipocytes to identify novel pathways regulating BAT. CHRM2 (encoding the muscarinic acetylcholine receptor 2) was the most differen...

ea0077oc3.1 | Metabolism, Obesity and Diabetes | SFEBES2021

The serotonin transporter SLC6A4 protects human brown adipose tissue from serotonin-mediated suppression of thermogenesis.

Choong Kwok T’ng , Suchacki Karla , Ramage Lynne , Kelman Alexandra , McNeill Ben , Rodney Stewart , Keegan Matthew , Gray Calum , Manning Jonathan , MacNaught Gillian , Fletcher Alison , Simpson Joanna , Carter Roderick , Morton Nicholas , Homer Natalie , van Beek Edwin , Wakelin Sonia , Stimson Roland

The recent discovery of brown adipose tissue (BAT) in adult humans, which generates heat to maintain body temperature in a cold environment, offers an exciting new strategy to treat obesity and metabolic disease, but our knowledge of human BAT activation is limited. To identify novel pathways regulating human BAT, we undertook RNA sequencing of human brown and white adipocytes. The gene SLC6A4 (encoding the serotonin transporter SERT) was one of the most highly differ...

ea0086oc5.1 | Metabolism, Obesity and Diabetes | SFEBES2022

Human brown adipose tissue demonstrates substantial choline uptake for incorporation into phosphatidylcholines

Suchacki Karla , Ramage Lynne , Gray Calum , Rodrguez Blanco Giovanny , Choong Kwok T'ng , Boyle Luke , MacNaught Gillian , Gregoriades Maria-lena , Wakelin Sonia , von Kriegsheim Alex , Finch Andrew , Patel Dilip , van Beek Edwin , Stimson Roland

Background: 18F-fluorodeoxyglucose (18FDG) PET is commonly used to quantify brown adipose tissue (BAT) mass/activity in humans but requires cold exposure. Rodent brown (BAds) but not white adipocytes (WAds) exhibit high choline content, thus we hypothesised that human BAT would demonstrate substantial 18F-fluorocholine (18FCH) uptake in vivo during warm and cold conditions.Methods: (1)...