Searchable abstracts of presentations at key conferences in endocrinology

ea0019p314 | Steroids | SFEBES2009

The adipokine, Pre-B cell colony enhancing factor (PBEF)/visfatin, activates steroidogenic acute regulatory protein (StAR) protein expression and steroid production in human adrenocortical-H295R-cells via MAPK and PI3/AkT signalling pathways

Kaur Jaspreet , Ramanjaneya Manjunath , Chen Jing , Randeva Harpal

PBEF/Visfatin is an adipokine highly enriched in adipose tissue which is increased in obesity and has been shown to be involved in numerous physiological processes. The adrenal gland plays a pivotal role in the integration of metabolic activity and energy balance, through production of steroids, mainly glucocorticoids. Steroidogenesis is a tightly controlled and essential process, which is mediated by steroidogenic acute regulatory protein (StAR), a protein that transports cho...

ea0016p665 | Signal transduction | ECE2008

Signalling properties of corticotropin-releasing hormone receptor (CRH-R1/2) in adrenal (H295R) cells

Punn Anu , Ramanjaneya Manjunath , Lehnert Hendrik , Grammatopoulos Dimitris

Corticotropin-releasing hormone (CRH) and urocortins (UCN) bind to two types of GPCRs (CRH-R1 and CRH-R2), activating a plethora of signalling cascades, including the mitogen-activated protein kinases (MAPKs) pathways. Although previous studies have identified that CRH directly regulates adrenal steroidogenesis1, the second messenger systems and downstream effectors remain poorly defined. The adrenal cell line H295R, which express both CRH-Rs1 was used to...

ea0034p263 | Obesity, diabetes, metabolism and cardiovascular | SFEBES2014

Renalase a key regulator of brown adipose tissue activity

Ramanjaneya Manjunath , Tan Bee , Patel Vanlata , Hu Jiamiao , Lehnert Hendrik , Hillhouse Edward , Chen Jing , Randeva Harpal

Renalase is a recently discovered flavin adenine dinucleotide (FAD)-dependent oxidase, predominantly secreted into the circulation from the kidney and regulates catecholamine degradation and metabolism. Renalase gene is also present in heart muscle, skeletal muscle and liver cells in humans, and in mouse testicles. Four isoforms of renalase gene has been identified in humans (hRenalase1 to hRenalase4), however only hRenalase1 is detected in human blood suggesting that hRenalas...

ea0021p153 | Diabetes and metabolism | SFEBES2009

A role for aquaglyceroporins in adipogenesis

Brown James , Conner Matthew , Bill Roslyn , Ramanjaneya Manjunath , Bailey Clifford , Randeva Harpal , Conner Alex

Aims/objectives: Glycerol cycling plays an essential regulatory role in metabolism in both health and disease. Aquaporins (AQPs) are a recently described family of water transporting membrane proteins, which contain a subset of members that also have the ability to transport glycerol across the cell membrane. This study sought to investigate the adipose tissue expression profile of AQPs in humans, and to assess the role of AQPs in adipocyte differentiation.<p class="abstex...

ea0019oc7 | Young Endocrinologist prize session | SFEBES2009

In vivo and ex vivo regulation of Visfatin production by leptin in human and murine adipose tissue: role of the PI3K and MAPK pathways

Tan Bee , Chen Jing , Brown James , Adya Raghu , Ramanjaneya Manjunath , Bailey Cliff , Lehnert Hendrik , Randeva Harpal

Apart from its role in energy storage, adipose tissue produces several hormones and cytokines termed ¬Ďadipokines¬í that have wide-ranging effects on carbohydrate and lipid metabolism; and thereby, play an important role in the pathogenesis of the metabolic syndrome. Visfatin is a novel adipokine, levels of which increase in obesity and is adipogenic; properties common to that of leptin. Thus, leptin may modulate visfatin production in adipose tissue. We investigated t...

ea0019p131 | Diabetes, Metabolism and Cardiovascular | SFEBES2009

Identification of nesfatin-1/NUCB2 as a novel depot-specific adipokine in human and murine adipose tissue: aletered levels in obesity and food deprivation

Ramanjaneya Manjunath , Chen Jing , Brown James , Patel Suketu , Tan Bee , Randeva Harpal

NESFATIN-1 is a recently identified hypothalamic satiety molecule derived from its precursor protein, NEFA/nucleobindin2 (NUCB2). Although the hypothalamus is considered the cornerstone for maintenance of energy homeostasis, adipose tissue plays an important role in the integration of metabolic activity and energy balance; communicating with peripheral organs and the brain via secreted proteins, termed adipokines. Many hypothalamic satiety regulatory peptides have been describ...

ea0016p657 | Signal transduction | ECE2008

Orexins stimulate steroidogenic acute regulatory (StAR) protein expression through multiple signalling pathways

Ramanjaneya Manjunath , Conner Alex , Chen Jing , Lehnert Hendrik , Stanfield Peter , Randeva Harpal

Orexins mediate a variety of physiological processes including feeding behaviour, the circadian pathway and cortisol secretion. Steroidogenesis is regulated by a variety of neuropeptides and one of the key rate-limiting steps is cholesterol transport across the mitochondrial membrane by the steroidogenic acute regulatory (StAR) protein. StAR expression can be regulated through several different signalling pathways. Despite the clear link between orexins and steroid production,...

ea0019p315 | Steroids | SFEBES2009

Orexin-stimulated MAP Kinase cascades are activated through multiple G-protein signalling pathways in human H295R adrenocortical cells: differential regulation by orexin A and orexin B

Chen Jing , Ramanjaneya Manjunath , Conner Alex , Kumar Prashanth , Brown James , Johren Olaf , Lehnert Hendrik , Stanfield Peter , Randeva Harpal

Orexin-A and orexin-B orchestrate their diverse central and peripheral effects via two G-protein coupled receptors, OX1R and OX2R, which activate multiple G-proteins. They are involved in a host of physiological processes including, steroidogenesis, appetite control and energy regulation. Whilst some signalling mechanisms have been proposed for individual recombinant orexin receptors in generic mammalian cell types, it is clear that the peripheral effects of orexin are spatial...

ea0059p170 | Obesity &amp; metabolism | SFEBES2018

Effects of visfatin on brown adipose tissue energy regulation

Dimitriadis Georgios K , Adya Raghu , Tan Bee K , Jones Terence A , Menon Vinod S , Ramanjaneya Manjunath , Kaltsas Gregory , Miras Alexander D , Randeva Harpal S

The role of brown adipose tissue (BAT) in pathological states of energy homeostasis and impaired adipocyte function, such as obesity has been a major area of research interest in recent years. Herein, we sought to determine the direct effects of adipokines, visfatin and leptin on BAT thermogenesis. The effects of mouse recombinant visfatin, nicotinamide mononucleotide (NMN) and leptin with or without FK866 were studied on differentiated T37i cells. Treated cells were analyzed ...