Searchable abstracts of presentations at key conferences in endocrinology

ea0028p136 | Growth and development | SFEBES2012

Maturational effects of glucocorticoids on fetal cardiomyocytes are direct and mediated by glucocorticoid receptor

Rog-Zielinska Eva , Kenyon Chris , Holmes Megan , Chapman Karen

In addition to their essential role in fetal lung development, glucocorticoids promote late-gestation maturation of the fetal heart as shown by severe functional impairment, structural disorganisation and transcriptome immaturity in fetal hearts of glucocorticoid receptor-deficient (GR-/-) mice. Here we use primary fetal mouse cardiomyocytes (E16-E17.5) to investigate whether these effects of glucocorticoids result from direct actions of GR and whether they are independent of ...

ea0034oc2.4 | Endocrine regulation of cell behaviour | SFEBES2014

Influence of glucocorticoid receptor density on development and remodeling of the heart

Richardson Rachel , Rog-Zielinska Eva , Thomson Adrian , Moran Carmel , Gray Gillian , Chapman Karen

Variation in the human glucocorticoid receptor (GR) gene associates with relative glucocorticoid resistance, hypertension and increased cardiovascular disease risk. Mice heterozygous for a null GR mutation (GR+/−) are also glucocorticoid resistant with raised circulating glucocorticoid levels and elevated blood pressure in adulthood. We have characterised the cardiac phenotype of these mice throughout development and investigated their response to cardiovascul...

ea0031oc4.1 | Obesity, metabolism and bone | SFEBES2013

Glucocorticoid receptor deficiency in cardiomyocytes causes pathological cardiac remodelling in mice

Richardson Rachel , Rog-Zielinska Ewa , Thomson Adrian , Moran Carmel , Kenyon Christopher , Gray Gillian , Chapman Karen

Variation in the glucocorticoid receptor (GR) gene associates with relative glucocorticoid resistance, hypertension and increased cardiovascular disease risk in humans. To investigate the contribution of cardiac GR to this phenotype we have characterised adult male mice with cardiomyocyte and vascular smooth muscle deletion of GR (SMGRKO) and have found left ventricular function to be impaired.SMGRKO mice, generated by crossing GR ‘floxed’ mice...

ea0031p141 | Growth and development | SFEBES2013

Impaired heart function and cardiac maturation in fetal mice with disrupted GR signalling in vascular smooth muscle and cardiomyocytes

Rog-Zielinska Eva A , Thompson Adrian , Moran Carmel , Kenyon Christopher J , Holmes Megan C , Chapman Karen E

Glucocorticoid signalling is essential for cardiac maturation late gestation. In mice, global glucocorticoid receptor deficiency (GR−/−) severely impairs cardiac function and ultrastructure at embryonic day (E) 17.5. To dissect direct effects of GR deficiency in the heart from effects on other systems, Sm22α-Cre mice were crossed with ‘floxed’ GR mice to generate SMGRKO mice with disrupted GR signalling in cardiomyocytes and vascular smoot...

ea0038oc4.6 | Diabetes and cardiometabolic complications | SFEBES2015

Cardiac fibrosis and the balance between glucocorticoid and mineralocorticoid receptors signalling

Richardson Rachel , Batchen Emma , Darroch Rowan , Scullion Kathleen , Rog-Zielinska Ewa , Kenyon Christopher , Gray Gillian , Chapman Karen

Specific variations in the human glucocorticoid receptor (GR) gene associate with increased cardiovascular disease risk. GR signalling is essential for cardiac maturation in utero and adult mice with cardiomyocyte and vascular smooth muscle deletion of GR (SMGRKO mice) have cardiac hypertrophy, fibrosis and impaired function. Intriguingly, levels of left ventricle (LV) mRNA encoding the mineralocorticoid receptor (MR), which is pro-fibrotic in heart, rise postnatally ...

ea0073aep650 | Thyroid | ECE2021

Myxedema coma in a COVID-19 patient

Cidade-Rodrigues Catarina , Dalila Martins Marta , Verónica Cardoso , Linhas Rita , Viveiros Filipa , Rogério Ruas , Ferraz Rita , Cunha Filipe

IntroductionMyxedema coma is a rare condition with an estimated incidence of 0.22 per million per year in the western world and a mortality rate around 30–50%. It can occur as the result of severe longstanding hypothyroidism or be precipitated by an acute event, such as infection. We present a rare case of myxedema coma in an elderly woman with SARS-CoV-2 infection.Case reportAn 82-year-old woman, with n...

ea0025oc5.4 | Reproduction and fetal programming | SFEBES2011

Impaired cardiac function in GR−/− fetal mice

Rog-Zielinska Eva , Thomson Adrian , Moran Carmel , Brownstein David , Kenyon Christopher , Michailidou Zoi , Szumska Dorota , Bhattacharya Shoumo , Richardson Jennifer , Owen Elizabeth , Watt Alistair , Forrester Lesley , Holmes Megan , Chapman Karen

Glucocorticoid levels rise dramatically in late gestation in mammals and are essential for organ maturation in preparation for birth. They are widely used clinically to mature the lungs of premature infants and hypomorphic GR mice die neonatally due to severe lung atelectasis. Here, we describe fetal lethality in GR null (GR−/−) mice with 51% loss at E17.5 (P<0.05) that could be due to impaired cardiac development. Histology showed reduced hea...