Searchable abstracts of presentations at key conferences in endocrinology

ea0059p065 | Clinical biochemistry | SFEBES2018

An oestrogen profiling mass spectrometry method using N-Methyl Pyridine-3-sulfonyl chloride derivatisation

Johal Jamine , Jolly George , Gilligan Lorna C , Taylor Angela E

Objectives: Oestrogen analysis using liquid chromatography mass spectrometry is problematic, as oestrogens do not readily ionise. This coupled with low concentrations in men, pre-pubertal and post-menopausal women provides an analytical challenge. We investigated N-Methyl Pyridine-3-sulfonyl chloride (NMPS) derivatisation, as described by Wang et al. (Steroids 2015 Apr;96:140-152) to improve sensitivity of 11 oestrogens; oestrone (E1), oestradiol (E2), 2-hydroxy-oestr...

ea0035s26.3 | Pitfalls in hormone measurement | ECE2014

Advantages in the measurement of steroid hormones

Taylor Angela E , ONeil Donna M , Arlt Wiebke

Mass spectrometry has been used to measure steroids for more than 40 years, but has gained popularity within the endocrine field in the last 15+ years. This is mainly due to technological advances that facilitated the development of high throughput methods.Historically, steroids were measured by crude techniques such as thin layer chromatography (TLC) and later by the more sophisticated RIA. The problems with these techniques include:<p class="abstex...

ea0081p698 | Reproductive and Developmental Endocrinology | ECE2022

The biochemical investigation of PCOS: a UK wide survey of laboratory practice

M Hawley James , Marrington Rachel , MacKenzie Finlay , Adaway Jo , Taylor Angela , Arlt Wiebke , Keevil Brian

Background: Polycystic ovary syndrome (PCOS) is a heterogenous condition that affects approximately 12% of females. The diagnosis can be complex and biochemistry tests are routinely relied upon to help identify hyperandrogenaemia and to exclude other conditions. However, although national and international guidelines advocate the use biochemistry tests, little information is provided as to which tests should be used and which other endocrinopathies should be excluded.<p cl...

ea0065p138 | Endocrine Neoplasia and Endocrine Consequences of Living with and Beyond Cancer | SFEBES2019

Targeting oestrogen synthesis and action as a novel therapy for colorectal cancer

Sidhu Daljit , Haime Hope , Pidd Aidan , Taylor Angela , Cox Liam , Foster Paul

Colorectal cancers (CRC) represent 9.4% of all cancers, with about 42 000 new UK cases per year. Current treatment involves surgical resection followed by radio- and chemo-therapy. 5-Fluorouracil is the standard post-operative chemotherapy although tolerance is poor due to dose-limiting toxicities and drug-resistance. New treatment strategies are therefore sorely needed. One emerging chemotherapeutic option is to target oestrogen metabolism. Our novel work rev...

ea0065p217 | Metabolism and Obesity | SFEBES2019

A novel method for analysis of 11 oestrogens using high-throughput liquid chromatography tandem mass spectrometry

Gilligan Lorna , Goymer Lauren , Toor Harjeavon , Cox Grace , Shaheen Fozia , Arlt Wiebke , Taylor Angela

Oestrogen quantification in serum is challenging as concentrations are low, especially in men and post-menopausal women. Additionally, oestrogens do not ionise readily and structure similarities can lead to cross-reactivity and reduced specificity, especially with immunoassay. Full characterisation of oestrogen metabolism and its role in disease progression has therefore not been fully investigated. We aimed to develop and validate a liquid chromatography mass spectrometry met...

ea0065p229 | Metabolism and Obesity | SFEBES2019

Steroid deconjugation by helix pomatia – can we overcome snail speed?

Shaheen Fozia , Gilligan Lorna , Berner Camila , Callejas Jose Luis , Shackleton Cedric , Arlt Wiebke , Taylor Angela

Gas chromatography–mass spectrometry (GC–MS) has been used for urinary steroid analysis for over 50 years. The process of hydrolysis to release steroids from their conjugates is routinely performed using commercially available snail Helix-Pomatia sulfatase (HP). However, as this is a naturally occurring enzyme its production process results in batch variation and so each batch requires experimental validation to adjust for the difference in efficiency of the enzymati...

ea0065p230 | Metabolism and Obesity | SFEBES2019

Acute changes in steroid biosynthesis in patients following severe trauma: the golden hour study

Taylor Angela , Bentley Conor , Foster Mark , Lord Janet , Hazeldine Jon , Arlt Wiebke

Advancements in medical care have significantly improved survival rates following major traumatic injury. An understanding of the hormonal, inflammatory and metabolic changes that occur following trauma is still evolving but it is clear that they impact significantly upon patient prognosis. To date, studies that have examined trauma-induced changes in steroid metabolism have analysed samples taken from patients post-hospital admission, culminating in marked variability in the ...

ea0044p67 | Clinical biochemistry | SFEBES2016

Three minute run time LC-MS/MS method for separation and quantifying 25-hydroxyvitamin D from C3-epimers

Jenkinson Carl , Bradbury James , Taylor Angela , He Shan , Viant Mark , Hewison Martin

Vitamin D exists as two forms; D3 (UV) and D2 (plant derived). Measuring the metabolite 25-hydroxyvitamin D (25OHD) is routinely applied in research and clinical laboratories to assess vitamin D status. The Institute of Medicine and Society for Endocrinology have previously set recommended vitamin D guidelines based on combined 25OHD3 and 25OHD2 serum concentrations. In order to achieve accurate quantitation of these metabolites, the respective C3 epimers must be separately qu...

ea0041oc9.3 | Endocrine Tumours | ECE2016

Stressed to death – antioxidant pathway targeting as a novel therapeutic approach in adrenocortical carcinoma

Chortis Vasileios , Taylor Angela E , Doig Craig L , Meimaridou Eirini , Metherell Louise A , Arlt Wiebke , Foster Paul A

Context: Nicotinamide nucleotide transhydrogenase (NNT) is a NADPH-generating mitochondrial proton pump with a central role in mitochondrial antioxidant pathways. Recent studies revealed inactivating NNT mutations in patients with familial glucocorticoid deficiency, indicating a selective susceptibility of the adrenal cortex to NNT deficiency and oxidative stress. Here we explored the potential value of NNT as a therapeutic target in adrenocortical cancer.<p class="abstext...

ea0041gp184 | Reproduction &amp; Endocrine Disruption | ECE2016

Impaired DHEA sulfation defines androgen excess in women with polycystic ovarian syndrome (PCOS)

Kempegowda Punith , O'Reilly Michael W , Hassan-Smith Zaki , Storbeck Karl-Heinz , Taylor Angela E , Arlt Wiebke

Context: Polycystic ovarian syndrome (PCOS) is the most common endocrine disorder in women of classic androgen synthesis. Recent work has highlighted that impaired DHEA sulfation results in enhanced androgen production. Here, we evaluated the relationship between DHEA and DHEAS and androgen excess and metabolic phenotype in PCOS.Patients and methods: We compared 65 women with PCOS to 35 healthy women matched for age and body mass index (BMI). All subject...