Searchable abstracts of presentations at key conferences in endocrinology

ea0065p195 | Metabolism and Obesity | SFEBES2019

ISX-9 preferentially induces enterochromaffin and I-cell enteroendocrine lineages in human small intestinal organoids

Pedro Patricia Fonseca , Tsakmaki Anastasia , Bewick Gavin

Enteroendocrine cells (EECs) are a hormone-/neurotransmitter-producing population with well-defined physiological roles. Knowledge regarding their differentiation program in the human gut, however, is scarce. Deciphering endocrine specification could identify targets which allow the manipulation of specific EEC populations and form the basis for new treatments for metabolic, inflammatory and cognitive disorders. Isoxazole-9 (ISX-9) is a small molecule, previously used in proto...

ea0059p155 | Obesity & metabolism | SFEBES2018

Identity and cell fate of Ngn3-expressing population in small intestinal organoids

Pedro Patricia Fonseca , Tsakmaki Anastasia , Bewick Gavin

The intestinal epithelium (IE) is populated by different cell types each with a unique set of functions. Each cell type is derived from a common progenitor, the stem cell. The hierarchy of epithelial cell fate is transcriptionally regulated for example, Notch signalling defines secretory versus absorptive destiny. Peptide hormone producing enteroendocrine (EE) cells are scattered throughout the epithelium where they integrate complex nutrient signals and respond by promoting m...

ea0065p218 | Metabolism and Obesity | SFEBES2019

Inhibition of nicotinamide phosphoribosyltransferase (NAMPT) prevents cytokine-mediated beta cell dysfunction

Egbase Daniel , Sayers Sophie , Evans Elizabeth , Butterworth Sam , Greally Joshua , Bewick Gavin , Tsakmaki Anastasia , Persaud Shanta , Caton Paul

Intracellular nicotinamide phosphoribosyltransferase (NAMPT) is the rate-limiting enzyme in the NAD salvage pathway. Abnormally elevated NAMPT can promote autoimmune and inflammatory processes. Many of these processes are key to type 1 diabetes (T1D) pathogenesis. Whilst NAMPT has been examined in other inflammatory diseases, the role of NAMPT in T1D remains unclear. To investigate the role of NAMPT in T1D, we used small molecule NAMPT modulators to determine the role of abnor...