Searchable abstracts of presentations at key conferences in endocrinology

ea0081mte1 | Personalized therapy in acromegaly | ECE2022

Personalized therapy in acromegaly

Zatelli Maria Chiara

Personalized medicine aims at providing indications concerning the management of each patient according to her/his specific needs and disease characteristics. Acromegaly, although a rare disease, has a profound impact on patientsÂ’ quality and expectancy of life, due to increased morbidity and mortality as compared to the general population in correlation mainly due to cardiovascular disease and cancer. Several clinical variables have been investigated as predictors of res...

ea0031s8.3 | Non functioning pituitary tumours (Supported by <emphasis role="italic">Endocrine-Related Cancer</emphasis> and the Pituitary Foundation) | SFEBES2013

Pathological markers of aggressive pituitary tumour behaviour

Zatelli Maria Chiara

Recent advances in molecular pathology have improved our knowledge on the pathogenesis of pituitary tumors, as well as on their growth potential, likelihood of recurrence, and prognosis. The development of reliable and prognostically informative methods of assessing tumor behavior is particularly important in pituitary tumors, where no precise correlation exists between morphology and clinical aggressiveness. Specific morphologic features (macroscopic invasion of the perisella...

ea0035p681 | Growth hormone IGF axis basic | ECE2014

Effects of two mTOR inhibitors on in-vitro models of GH-secreting pituitary adenomas

Bellio Mariaenrica , Gagliano Teresa , Zatelli Maria Chiara , Uberti Ettore Degli

Background: Gigantism and acromegaly are the main consequences of GH excess, mainly due to a pituitary adenoma. Surgery is the first therapeutic option, but also medical therapy is employed, being represented mostly by somatostatin analogues (SSA), that reduce both tumour mass and GH hypersecretion. However about 10% of patients is resistant to SSA.PI3K/Akt/mTOR pathway, activated by growth-factors such as IGF1, is important in regulating many cellular p...

ea0081p288 | Calcium and Bone | ECE2022

Tumour induced osteomalacia: 2 years treatment with burosumab

Aliberti Ludovica , Pontrelli Margherita , Verrienti Martina , Gagliardi Irene , Zatelli Maria Chiara , Ambrosio Maria Rosaria

Introduction: Tumor-induced osteomalacia (TIO) is a paraneoplastic syndrome due to an overproduction of fibroblast growth factor 23 (FGF23) by small and benign mesenchimal tumors. FGF23 increase causes hypophosphatemia, osteomalacia and muscle weakness. TIO is usually cured by tumour resection, but neoplasms may be unidentifiable/unresectable or the patient may refuse surgery. In these cases, medical treatment with high doses of oral phosphate and calcitriol is mandatory, even...

ea0081ep1190 | Late Breaking | ECE2022

NanoLuc® Binary Technology to explore the mechanism of action of a Magmas inhibitor

Borges de Souza Patricia , Gagliardi Irene , Ambrosio Maria Rosaria , Bondanelli Marta , Zatelli Maria Chiara

Introduction: Magmas encodes for an integral constituent of the TIM23 translocase complex located in the mitochondrial inner membrane that drives proteins from the intermembrane space into the mitochondrial matrix by functionally interacting with Tim14. We previously demonstrated that Magmas silencing is able to sensitize ACTH-secreting mouse pituitary adenoma cells to pro-apoptotic stimuli, reduce DNA synthesis, accumulate cells in G0/G1 phase with concomitant decrease in S p...

ea0063p38 | Adrenal and Neuroendocrine Tumours 1 | ECE2019

Influence of Tumor Microenviroment in response modulation to treatment in human BP-NENs

Bresciani Giulia , Ditsiou Angeliki , Cilibrasi Chiara , Vella Viviana , Giamas Georgios , Zatelli Maria Chiara , Gagliano Teresa

Introduction: Broncho-Pulmonary Neoplasms (BP-NENs) are rare neoplasms arising from neuroendocrine cells of the respiratory epithelium. Since previous studies in our lab have demonstrated the efficacy ofEverolimus, approved for BP-NENs treatment, and Dinaciclib, a Cyclins and CdKs inhibitor, on monolayer system we have investigated if a more complex tumour system could generate a change in drugs effects and cell resistance. Therefore, through the use of 3D culture model, we ha...

ea0063p40 | Adrenal and Neuroendocrine Tumours 1 | ECE2019

Available 3D cultures methods: study on a Pancreatic Neuroendocrine Neoplasm cell line

Bresciani Giulia , Hofland Leo J , Giamas Georgios , Gagliano Teresa , Zatelli Maria Chiara

Background: Pancreatic Neuroendocrine Neoplasms (pNENs) are malignancies arising from the endocrine pancreas. Past in vitro studies have led to a better comprehension and characterization of this malignancy under several points of view. However, effective medical therapies are still not available, therefore, it is essential to continue studying this malignancy in order to identify a successful approach. Due to tumour complexity, techniques such as 3D cultures, able to...

ea0035oc7.1 | IGF-1 and Thyroid Basic | ECE2014

GH induces chemoresistance in human endometrial cancer cell lines involving ERK 1/2 and PKCdelta

Zuolo Giulia , Minoia Mariella , Gentilin Erica , Gagliano Teresa , Tagliati Federico , Zatelli Maria Chiara , Uberti Ettore degli

Background: Surgery is the main therapeutic option for advanced endometrial cancer; however, when disease relapses, chemotherapy is the only option. Chemoresistance is a very common phenomenon in these tumors. We previously demonstrated that GH protects breast cancer cells towards the cytotoxic effects of doxorubicin, inducing chemoresistance. Recent evidences show that endometrial cancer cells secrete GH, stimulating their own growth in an autocrine fashion.<p class="abst...

ea0035p538 | Endocrine tumours and neoplasia | ECE2014

Cyclin D1 levels are involved in the resistance to m-TOR inhibitors in human bronchial carcinoids

Benfini Katiuscia , Gagliano Teresa , Gentilin Erica , Minoia Mariella , Daniela Mole , Uberti Ettore degli , Zatelli Maria Chiara

Background: Bronchial carcinoids (BC) are still orphan of medical therapy. We previously demonstrated that the typical BC human cell line NCI-H727 is sensitive to Everolimus, in terms of cell viability reduction, while the atypical human BC cell line NCI-H720 is not. However, the mechanisms underlying this phenomenon have not been fully clarified.Aim: The aim of our study is to investigate the mechanisms of resistance to mTOR inhibitors in BC cells.<...

ea0032p6 | Adrenal cortex | ECE2013

Activation of EGFR promotes ACC cell proliferation by inducing VEGF autocrine secretion

Gagliano Teresa , Mole Daniela , Gentilin Erica , Bellio Mariaenrica , degli Uberti Ettore , Zatelli Maria Chiara

Adrenocortical cancer (ACC) is a rare and aggressive malignancy. Currently the main therapeutic option is surgery, but due to difficult and delayed diagnosis and to the onset of metastases, medical therapy is often tried. ACC treatment is mainly represented by Mitotane alone or in association with chemotherapy, with variable results. Understanding the molecular mechanisms that regulate ACC proliferation could be useful to identify new therapeutic options. Aim of our study is t...