Searchable abstracts of presentations at key conferences in endocrinology

ea0031p188 | Obesity, diabetes, metabolism and cardiovascular | SFEBES2013

Human abdominal subcutaneous adipocytes as an active source of LpPLA2, influenced by fat depot and metabolic state, with LpPLA2 converting LDL into more potent atherogenic Ox-LDL, in vitro

Kumsaiyai Warunee , Harte Alison , Al-Naji Fadi , Al-Daghri Nasser , Kyrou Ioannis , Barber Thomas , Sabico Shaun , Tripathi Gyanendra , McTernan Philip

Lipoprotein-associated phospholipase A2 (LpPLA2) is a member of the phospholipaseA2 super family of enzymes, and is upregulated in arterial inflammation, obesity and cardiovascular disease. The other isoforms, iPLA2 and cPLA2, appear to contribute to inflammation through production of lipid mediators. The role of PLA2 in human adipose tissue (AT) is unclear, therefore we sought to i) characterise PLA2 isoforms in lean, obese, T2DM abdominal subcutaneous (AbdSc) and omental (Om...

ea0031p205 | Obesity, diabetes, metabolism and cardiovascular | SFEBES2013

Irisin as a central regulator in energy homeostasis?

Piya Milan K , Harte Alison L , Sukumar Kavitha , James Sean , Tripathi Gyanendra , Kumar Sudhesh , Vatish Manu , McTernan Philip G

Background/objectives: Irisin is a novel myokine, released predominantly by skeletal muscle. Peripheral action of irisin improves glucose homeostasis, increases energy expenditure and induces browning of adipocytes, with therapeutic potential for use in weight loss. However, there are no human studies on the central role of irisin in the regulation of appetite or energy expenditure. This study sought to examine the potential central role of irisin, by demonstrating irisin in h...

ea0031p210 | Obesity, diabetes, metabolism and cardiovascular | SFEBES2013

Maternal B12 insufficiency predicts neonate's metabolic risk factors

Adaikalakoteswari Antonysunil , Alexander Lawson , Webster Craig , Wood Catherine , Anderson Neil , Sivakumar Kavitha , McTernan Philip , Tripathi Gyanendra , Vatish Manu , Saravanan Ponnusamy

Studies in India (vegetarian population) show that vitamin B12 insufficiency is common in pregnancy and independently predict adiposity and insulin resistance in the offspring. Epigenetic programming is postulated as, along with folic acid, B12 is crucial for DNA methylation. Therefore, we investigated whether maternal B12 levels in a non-vegetarian UK population predict metabolic risk of the offspring.Paired maternal venous and cord blood samples (n...

ea0031p222 | Obesity, diabetes, metabolism and cardiovascular | SFEBES2013

Vitamin D supplementation as influenced by diabetic therapies

Alkharfy Khalid , Al-Daghri Nasser , Al-Othman Abdulaziz , Moharram Osama , Alokail Majed , Al-Saleh Yousef , Sabico Shaun , McTernan Philip

Objective: Vitamin D deficiency is associated with an increased risk of type two diabetes mellitus (T2DM) and cardiovascular disease reduced through Vitamin D3 supplementation. However to the best of our knowledge, no research has determined the effect of vitamin D3 supplementation in conjunction with existing pharmacological and non-pharmacological approaches to the DMT2 population. Hence, the aim of this study was to determine the effect of vitamin D3 supplementation in a co...

ea0028p176 | Obesity, diabetes, metabolism and cardiovascular | SFEBES2012

Expression of genes regulating mitochondrial fusion and fission in human adipose tissue are influenced by adiposity and bariatric surgery

McCarthy Ciara , Voyias Philip , Antonysunil Adaikala , McGee Kirsty , Harte Alison , Saravanan Ponnusamy , Kyrou Ioannis , Kumar Sudhesh , McTernan Philip , Tripathi Gyanendra

Mitochondria are essential for synthesising ATP required for cellular metabolism. In response to changes in their cellular environment, mitochondria are able to alter their morphology and abundance through the balance of “fusion” and “fission” events. The aim of this study was to profile the expression of mitochondrial genes in the abdominal subcutaneous (AbSc) adipose tissue (AT) of lean, obese and Type 2 Diabetes Mellitus (T2DM) individuals and determine ...

ea0028p184 | Obesity, diabetes, metabolism and cardiovascular | SFEBES2012

The adipocyte as an active source of PLA2 isoforms influenced by adiposity, depot specificity and Type 2 Diabetes Mellitus

Kumsaiyai Warunee , Harte Alison , Saravanan Ponnusamy , Kyrou Ioannis , Aldaghri Nasser , Al-Attas Omar , Kumar Sudhesh , Tripathi Gyanendra , McTernan Philip

Lipoprotein-associated phospholipase A2 (Lp-PLA2) is a member of the phospholipase A2 super family of enzymes that hydrolyse phospholipids, and is upregulated in arterial inflammation, obesity and cardiovascular disease. Secreted soluble Lp-PLA2 (sPLA2) increases arterial inflammation in addition to cytosolic calcium dependent PLA2 (cPLA2) and calcium independent PLA2 (iPLA2). These studies examined the influence of adipose tissue (AT) depots (abdominal subcutaneous (Abd Sc), ...

ea0021p181 | Diabetes and metabolism | SFEBES2009

The influence of HIV status and antiretroviral (ARV) therapy on expression of genes related to mitochondrial metabolism in human adipose tissue (AT)

McGee Kirsty , Boothby Meg , Harte Alison , Tomlinson Jeremy , Hill Margaret , Gathercole Laura , Kumar Sudhesh , Shahmanesh Mohsen , McTernan Philip

Background and aims: The mechanisms in HIV by which mitochondrial dysfunction occurs, leading to impaired energy homeostasis and metabolic regulation, are unclear; although similarities are noted in T2DM. Therefore, we investigated the effect of HIV status and ARV therapy on several mitochondrial and nuclear encoded genes related to mitochondrial metabolism and energy homeostasis.Materials and methods: Subcutaneous AT biopsies from the ileac crest were o...

ea0015p134 | Diabetes, metabolism and cardiovascular | SFEBES2008

Endoplasmic reticulum stress and the innate immune signalling pathway in human abdominal adipose tissue: the effects of obesity

Kusminski Christine , Schisano Bruno , Harte Alison , Tripathi Gyanendra , Ceriello Antonio , O'Hare Joseph P , Kumar Sudhesh , McTernan Philip

Background and objectives: Obesity-associated inflammation is a key contributory factor in the pathogenesis of type 2 diabetes mellitus (T2DM). Murine studies have revealed potential links between obesity-induced activation of the innate immune response and endoplasmic reticular (ER) stress. Our aims were therefore to (1) examine whether human abdominal subcutaneous (AbSc) adipose tissue (AT) obtained from lean and obese patients, differs in the level of ER stress, through ass...

ea0013p147 | Diabetes, metabolism and cardiovascular | SFEBES2007

Adiponectin complexes in human cerebrospinal fluid: distinct complex distribution from serum

Kusminski Christine , Kos Katarina , Schraw Todd , O’Hare Joseph , Ahima Rexford , Scherer Philipp , Kumar Sudhesh , McTernan Philip

Aims/hypothesis: Adiponectin is an adipocyte-derived secretory factor that is highly specifically expressed in adipocytes. It exerts effects on energy homeostasis via peripheral and central mechanisms. However, it is not clear whether adiponectin is crossing the blood brain barrier in humans. In serum, adiponectin circulates in several different complexes, each of which has distinct functions. Here, we wanted to test whether adiponectin can be found in human cerebrospinal flui...

ea0044p110 | Diabetes and Cardiovascular | SFEBES2016

Volatile organic compounds: A potential biomarker for prediction of hypertension

Azharian Sahar , Neal Matthew , Kyrou Iannos , Piya Milan , Kumar Sudesh , Covington James , McTernan Philip , Vrbikova J , Hainer V , Sramkova P , Fried M

Background: Human obesity can significantly contribute to hypertension risk, often referred to as the ‘silent killer’, although targeting whom with weight gain becomes clinically hypertensive is unclear. Whilst therapeutic intervention such as bariatric surgery can mitigate both obesity and hypertension, it is not the most suitable solution for all patients. Therefore an early biomarker detection system to assess hypertension in subjects with weight gain could reduce...