Searchable abstracts of presentations at key conferences in endocrinology
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10th European Congress of Endocrinology

Poster Presentations

Endocrine tumours

ea0016p271 | Endocrine tumours | ECE2008

The comparison of serum VEGF levels between patients with metastatic and non-metastatic thyroid cancer, and patients with non-toxic multinodular goiter

Klubo-Gwiezdzinska Joanna , Junik Roman

One of the important proangiogenic factors involved in the growth of normal and neoplastic tissues is vascular endothelial growth factor VEGF.Therefore we hypothesized, that serum VEGF concentration would differ between patients with metastatic and non-metastatic thyroid cancer, with multinodular goiter and healthy subjects. We also hypothesized that endogenous TSH stimulation would effect serum VEGF level. The study protocol was approved by the Ethical ...

ea0016p272 | Endocrine tumours | ECE2008

Special multiple endocrine neoplasia (MEN)

Santiago Piedad , Ramirez Cesar , Ruiz Isabel , Martinez Pilar , Lopez Jose Antonio , Sanchez Carolina , Moreno Alberto

The ‘Multiple Endocrine Neoplasia’ predominantly affect the pituitary gland, parathyroids, thyroids, adrenal glands and pancreas. We present the case of a patient with three different functioning endocrine gland tumours, but who cannot be included in any of these groups.Case report: Sixty-five-years-old female with recurrent episodes of nephritic colic and hypertension for 2 years; non-specific gastrointestinal complaints, hirsutism, hyp...

ea0016p273 | Endocrine tumours | ECE2008

The early results of the treatment of well differentiated thyroid cancer and its dependence on chosen factors

Klubo-Gwiezdzinska Joanna , Junik Roman

The aim of the study was to estimate the influence of a thyroid remnants’ volume, postsurgical concentration of thyroglobulin and radioiodine dose on the early treatment efficacy of well differentiated thyroid cancer.Material and methods: We retrospectively analyzed 91 patients (76 females, 15 men) with well differentiated thyroid cancer.Results: Histological classification revealed 68.1% (62/91) papillary thyroid cancers, 25....

ea0016p274 | Endocrine tumours | ECE2008

Variability in rensponse to octreotide in patients with insulinoma detected by 111In-octreotide scintigraphy (Octreoscan)

Diacono Fabrizio , Tamburrano Guido

Purpose: 111In-octreotide scinti aphy may be useful in patients with insulinoma during pre-surgical localization of the tumor and octreotide is effective in inhibiting insulin secretion and reducing the hypoglycemic events. The aim of the study was to evaluate 111in-octreotide scintigraphy in localizing primary and metastatic insulinomas and predicting the response to octreotide administration.Patients and methods: Diagnosis of insulinoma was made in 17 ...

ea0016p275 | Endocrine tumours | ECE2008

Nerve vagus schwannoma

Karatas Adem , Duren Mete , Karabulut Kagan , Hatemi Husrev , Ates Guler Turkes , Giray Serdar

Introduction: Schwannomas are benign, encapsulated, solitary, slow-growing tumors which originate from nerve sheath cells in cranial, periferic, symphathetic nerve system. Aproximately, 25–45% of schwannomas are in head and neck region. N. Vagus Schwannoma are seen relatively, rarely. The patients frequently apply with a slow-growing, painless servical mass. Malign transformation is unusual.Case report: The patient 37 years old applied to our clinic...

ea0016p276 | Endocrine tumours | ECE2008

Use of multispine computed tomography in diagnostics of ectopic acth-syndrome

Maria Babarina , Evgenia Marova , Peter Yushkov , Ludmila Rozhinskaya

Aim: To access the efficiency of mulitispine computed tomography (MSCT) in ectopic ACTH-syndrome.Patients and methods: Five clinical cases (2 female and 3 male cases) with severe hypercortisolism were analyzed, age from 26 to 63 years old.Results: In all of the 5 cases there were significant reasons to suppose ectopic ACTH-syndrome: rapid development of clinical features (in 4 of 5 cases), loss of lean mass compound with rounded an...

ea0016p277 | Endocrine tumours | ECE2008

Rottlerin inhibits migration of thyroid follicular carcinoma through destablization of the focal adhesion complex

Lin Chieh-Yu , Lin Chien-Jung , Huang Shih-Horng , Wu Jiann-Chun , Wang Seu-Mei

The ability of local invasion and distant metastasis indicates malignancy. Focal adhesion complex plays an important role during cell invasion. This complex consists of integrins, focal adhesion kinase (FAK), vinculin, talin, α-actinin and paxillin. Activation of FAK regulates the assembly of focal adhesion and stress fiber formation via GTPase Rho and Rac, which is responsible for the migratory behavior of cells. In this study, we demonstrated the inhibitory effect of ro...

ea0016p278 | Endocrine tumours | ECE2008

The enzymatic activity of type 1 iodothyronine deiodinase (D1) is low in large intestine metastases into liver

Debski Marcin , Kornasiewicz Oskar , Stepnowska Marta , Szalas Anna , Krawczyk Marek , Bar-Andziak Ewa

Type 1 iodothyronine deiodinase (D1) is responsible for the conversion of thyroxin (T4) into tri-iodothyronine (T3). The enzyme is mainly present in thyroid, liver and kidneys.There is strong evidence that the metabolism of thyroid hormones is disturbed in some neoplasmatic tissues. However there are only few available data about D1 enzymatic activity in liver tumors.The aim of this study was to estimate the e...

ea0016p279 | Endocrine tumours | ECE2008

PTHrP and HRG outcomes in MCF7 breast cancer cells transfected with HER receptors gene

Alokail Majed

Metastasis to bone occurs frequently in advanced breast cancer and is accompanied by debilitating skeletal complications. Parathyroid hormone-related protein (PTHrP) occurs in a high proportion of breast cancer and is strongly implicated in their metastatic spread to bone. Overexpression of PTHRP and its receptor in breast tumour cells could also promote the growth in an autocrine fashion. Signal transduction of growth factor receptor, EGFR (ErbB1, HER1) and ErbB2 (Neu, HER2) ...

ea0016p280 | Endocrine tumours | ECE2008

Type 1 iodothyronine deiodinase (D1) enzymatic activity is not reduced in liver focal nodular hyperplasia (FNH)

Szalas Anna , Stepnowska Marta , Debski Marcin , Kornasiewicz Oskar , Krawczyk Marek , Bar-Andziak Ewa

FNH is the second most common benign liver tumor caused mainly by oral contraceptives. D1, which is a crucial enzyme in catalyzing prohormone thyroxine (T4) into active triiodothyronine (T3), is mainly present in such tissues as thyroid, liver and kidney. There are only few data about D1 enzymatic activity in neoplasmatic conditions. Mostly in tumors reduced D1 activity was found.The aim of the study was to examine the activity of t...

ea0016p281 | Endocrine tumours | ECE2008

An unusual case of painful gynaecomastia due to large adrenocortical tumour

Lakshmi Santhosh , Foote John

We present an unusual case of painful gynaecomastia due to a large adrenocortical tumour secreting oestradiol and other steroid hormones.A 46 years old man presented with a 6 months history of progressive, painful gynaecomastia. He had no other specific symptoms and had previously been well. Alcohol intake was not excessive. Interestingly, his sister had presented with a phaeochromocytoma 2 years previously.Examination demonstrated...

ea0016p282 | Endocrine tumours | ECE2008

Screening of MEN1 gene in patients with either classic or variant MEN1 presentation

Cordella Daniela , De Marco Alessandro , Eller-Vainicher Cristina , Bastagli Anna , Jaffrain-Rea Marie Lise , Beck-Peccoz Paolo , Persani Luca

Multiple endocrine neoplasia type 1 (MEN1) is a rare dominantly inherited neoplastic syndrome. Tipically, it affects three major locations: parathyroid, endocrine pancreas or duodenum (GEP) and anterior pituitary. MEN1 is caused by mutations in MEN1 gene and its testing is now used as a complement to clinical diagnosis which may be hindered by the variable penetrance and expression of the defects. Mutation carriers are life-long monitored, while unaffected relatives can avoid ...

ea0016p283 | Endocrine tumours | ECE2008

Effect of treatment with depot somatostatin analogue octreotide on primary hyperparathyroidism in MEN-1 patients

Faggiano Antongiulio , Tavares Lidice , Milone Francesco , Mansueto Gelsomina , Ramundo Valeria , De Caro Maria Laura Del Basso , Lombardi Gaetano , De Rosa Gaetano , Colao Annamaria

Background: Expression of somatostatin receptor (SST) and therapy with somatostatin analogues have been scarcely investigated in parathyroid tumors.Objective: To evaluate the effects of depot long acting octreotide (OCT-LAR) on primary hyperparathyroidism in patients affected with multiple endocrine neoplasia type 1 (MEN-1).Subjects and methods: Eight patients with a genetically confirmed MEN-1 were enrolled. All patients presented...

ea0016p284 | Endocrine tumours | ECE2008

Correlation between postoperative hypothyroidism and seric homocysteine at the patients with thyroid carcinoma

Goldstein Andrei Liviu , Alexiu Florin , Purice Mariana

Carcinoma of the thyroid gland is an uncommon cancer but is the most common malignancy of the endocrine system. The treatment of the thyroid cancer includes surgery, radioactive iodine and hormone treatment. Postoperative hypothyroidism is the most common complication of thyroidectomy for thyroid cancer. After thyroidectomy, the patient takes a thyroid hormone drug (levothyroxine) in supresive dose for the rest of his life.In hypothyroidism there has bee...

ea0016p285 | Endocrine tumours | ECE2008

The role of parathyroid hormone-related protein on gynecomatia in patients with Klinefelter's syndrome and idiopathic gynecomastia

Deniz Ferhat , Bolu Erol , Azal Omer , Ozata Metin , Kutlu Mustafa

Background: Parathyroid hormone-related protein (PTHrP) was discovered as a tumor product. It is also one of the complex epithelial and mesenchymal interaction signal mediator in breast development. An increase in the prevalence of malignancy in patients with Klinefelter’s syndrome, but not in patients with idiopathic gynecomastia has been previously reported. PTHrP may play a critical role in the development or progression of breast cancer.Aim: We ...

ea0016p286 | Endocrine tumours | ECE2008

Two new mutations in the RET protooncogene: R770Q in coincidence with Y791N in the same family with medullary thyroid carcinoma

Rondot Susanne , Lorenz Angela , Schulze Egbert , Dralle Henning , Raue Friedhelm , Frank-Raue Karin

Context: Clinical studies are needed to classify rare and novel RET mutations associated with hereditary medullary thyroid carcinoma (MTC) into one of three clinical risk groups.Objective: We analyzed genotype–phenotype correlations associated with the RET protooncogene mutation R770Q in exon 13 which was detected simultaneously with a Y791N mutation in the same family.Results: Calcitonin determination in a 43-year-old female ...

ea0016p287 | Endocrine tumours | ECE2008

The role of 2-[18F]-fluoro-2-deoxy-D-glucose positron emission tomography (FDG/PET-CT) in the follow-up of differentiated thyroid cancer (DTC)

Pagano Loredana , Klain Michele , Pulcrano Melania , Ippolito Serena , Lombardi Gaetano , Salvatore Marco , Biondi Bernadette

Aim: To address the role of FDG/PET-CT in the follow-up of DTC.Methods: About 110 consecutive patients (86 female, 24 male, mean age 45±13 years), with DTC were selected between 1999 and 2006. All patients underwent total thyroidectomy, radioiodine ablation and had undetectable serum thyroglobulin (Tg) during L-T4 suppressive therapy and negative serum TgAb. The follow-up included a yearly clinical examination with n...

ea0016p288 | Endocrine tumours | ECE2008

Effects of the potent deacetylase inhibitor Panobinostat (LBH589) against poorly differentiated and anaplastic thyroid cancer cells

Catalano Maria Graziella , Pugliese Mariateresa , Bosco Ornella , Fortunati Nicoletta , Boccuzzi Giuseppe

Poorly differentiated and anaplastic thyroid carcinomas are aggressive human cancers that are resistant to conventional therapy. DAC inhibitors (DACi) are a promising class of drugs, acting as anti-proliferative agents by inducing apoptosis and cell cycle arrest.Panobinostat (LBH589) is a potent DACi belonging to a structurally novel cinnamic hydroxamic acid class of compounds, able to inhibit the activity of different isoforms of HDACs (HDAC 1, 3–6...

ea0016p289 | Endocrine tumours | ECE2008

Cytoplasmic shift of AUF1 in thyroid carcinoma

Trojanowicz Bogusz , Brodauf Lars , Sekulla Carsten , Chen Zhouxun , Bialek Joanna , Radestock Yvonne , Hombach-Klonisch Sabine , Klonisch Thomas , Finke Rainer , Dralle Henning , Hoang-Vu Cuong

AUF1/heterogeneous nuclear ribonucleoprotein D (hnRNPD) is an adenylate uridylate-rich elements (ARE) binding protein, which regulates the mRNA stability of many genes related to growth regulation, such as proto-oncogenes, growth factors, cytokines and cell cycle regulatory genes. Several studies demonstrated AUF1 expression in kidneys, liver, lymphoid tissues and melanocytes, and its involvement in apoptosis, tumorigenesis and development by its interactions with AREs bearing...

ea0016p290 | Endocrine tumours | ECE2008

Down-regulation of ENO1/MBP-1 gene products by retinoic acid (RA) causes decreased proliferation of the follicular thyroid carcinoma cell line FTC-133

Trojanowicz Bogusz , Winkler Anja , Hammje Kathrin , Chen Zhouxun , Sekulla Carsten , Glanz Dagobert , Schmutzler Cornelia , Mentrup Birgit , Hombach-Klonisch Sabine , Klonisch Thomas , Finke Rainer , Kohrle Josef , Dralle Henning , Hoang-Vu Cuong

Retinoic acid (RA) acts as an anti-proliferative and re-differentiation agent in the therapy of thyroid carcinoma but the molecular mechanisms by which RA mediates these effects are not well understood. We have investigated the effect of RA on the production and post-translational modification of the two ENO1 transcriptional products in the human follicular thyroid carcinoma cell line FTC-133. The single ENO1 transcript encodes a 48 kDa ENO1 with its unique N-terminal enolase ...

ea0016p291 | Endocrine tumours | ECE2008

Differences in the gene expression patterns of several immune related genes in various human adrenocortical tumors

Szabo Peter , Wiener Zoltan , Tombol Zsofia , Tolgyesi Gergely , Kovesdi Katalin , Farkas Roland , Horanyi Janos , Gaillard Rolf C , Falus Andras , Racz Karoly , Igaz Peter

Introduction: The adrenal cortex is highly involved at the immune-neuroendocrine interface, and several cytokines are known to influence the proliferation, differentiation, apoptosis and hormone production of adrenocortical cells. We therefore hypothesized that immune mediators might be involved in adrenal tumorigenesis, as well. Gene expression patterns of various inflammatory and immune mediators were studied by a functional genomics/bioinformatics approach.<p class="abs...

ea0016p292 | Endocrine tumours | ECE2008

Different gene expression patterns of histamine related genes in human adrenocortical tumors

Szabo Peter , Wiener Zoltan , Tombol Zsofia , Tolgyesi Gergely , Horanyi Janos , Gaillard Rolf C , Falus Andras , Racz Karoly , Igaz Peter

Introduction: The pathogenesis of sporadic adrenocortical tumors is poorly elucidated. Considering the importance of the adrenal cortex at the immune-neuroendocrine interface and the known actions of immune mediators on adrenocortical cell functioning, we supposed that immune or inflammatory factors might be involved in the pathogenesis of these tumors. Gene expression patterns of various inflammatory and immune mediators were studied by a functional genomics/bioinformatics ap...

ea0016p293 | Endocrine tumours | ECE2008

The prevalence of chosen complications in 72 acromegalics

Matyja Violetta , Kos-Kudla Beata , Foltyn Wanda , Strzelczyk Janusz , Karpe Jacek , Marek Bogdan , Sieminska Lucyna

Introduction: Hipersecretion of somatotrophin axis hormones in acromegaly favours the development of numerous metabolic and organ complications. The aim of the study was to establish the occurrence integrity of these complications in acromegalics.Material and methods: The tested 72 acromegalics – 32 men aged 46.9±15.2 years and 40 women aged 58.8±12.0 years, for which the period of the illness was determined, the body mass index (BMI) and ...

ea0016p294 | Endocrine tumours | ECE2008

Expression of the somatostatin receptors (SSTRs) in patients with gastro-pancreatic neuroendocrine tumours (GEP-NET) and medullary thyroid cancers (MTC)

Hubalewska-Dydejczyk Alicja , Szybinski Piotr , Trofimiuk Malgorzata , Stachura Jerzy , Wierzchowski Wojciech , Sowa-Staszczak Anna , Pach Dorota , Fross-Baron Katarzyna , Kulig Jan , Huszno Bohdan

The pattern of SSTRs expression in neuroendocrine neoplasms determines the possibility of the detection of the primary tumour and distant metastases, the application of radio-guided surgery (RGS) and the outcome of the treatment with itrium or lutetium labeled somatostatin analogues. The frequency and expression pattern of each subtype of SSTRs not only vary considerably in different NET but also in each patient.The aim of the study was to assess the SST...

ea0016p295 | Endocrine tumours | ECE2008

Melatonin restores the basal level of lipid peroxidation in rat tissues under conditions of exposure to potassium bromate

Stasiak Magdalena , Lewinski Andrzej , Karbownik-Lewinska Malgorzata

Potassium bromate (KBrO3) is a known prooxidant and carcinogen. Melatonin is a highly effective antioxidant. Indole-3-propionic acid (IPA) – an indole substance, and propylothiouracil (PTU) – an antithyroid drug, also reveal some antioxidative effects. The aim of the study was to evaluate KBrO3-induced lipid peroxidation in vitro in tissues collected from either control or melatonin-treated rats, and to compare potential preventive effect...

ea0016p296 | Endocrine tumours | ECE2008

Relaxin and S100A4 alter the invasive potential in estrogen-independent human MDA-MB-231 breast cancer cells

Radestock Yvonne , Hoang-Vu Cuong , Hombach-Klonisch Sabine

The heterodimeric peptide hormone relaxin is involved in extracellular matrix turnover during development and lactational differentiation of the breast. Relaxin expression is increased in human breast cancer and serum relaxin levels in breast cancer patients were reported to correlate with metastatic disease. We have established relaxin over-expressing transfectants of the highly invasive estrogen receptor-negative human breast cancer cell line MDA-MB-231 (MDA/RLN) to investig...

ea0016p297 | Endocrine tumours | ECE2008

The Relaxin peptide is a novel regulator of S100A4 (metastasin) in thyroid carcinoma cells

Radestock Yvonne , Hoang-Vu Cuong , Hombach-Klonisch Sabine

The peptide growth factor relaxin (RLN) is expressed in human thyroid carcinoma cell lines and tissues. We have previously established thyroid carcinoma cell transfectants with over-expression of relaxin and demonstrated that relaxin increases cellular motility and in-vitro invasiveness in these transfectants. This increase in motility and migration is relaxin receptor (LGR7)-dependent. S100A4 (metastasin), a member of the S100 family of calcium binding proteins, is known to c...

ea0016p298 | Endocrine tumours | ECE2008

Cyclic Cushing disease: clinical case

Santos Jacinta , Baptista Carla , Paiva Isabel , Rito Manuel , Carvalheiro Manuela

Cyclic Cushing’s disease is a rare situation due to episodic hypersecretion of ACTH. Suspicion is raised when strong clinical stigmata occur, with normal basal values of cortisol and normal responses to dynamic tests. After performing several tests, particularly during phases without symptoms (well-being), the probability of successful diagnosis increases.We describe the case of a 33 years old female patient with full-blown clinical picture (weight ...

ea0016p299 | Endocrine tumours | ECE2008

Adrenal ganglioneuroma: a new cause of increased serum calcitonin

Bakola Theodosia , Gkountouvas Anastasios , Aggelopoulou Anastasia , Tertipi Athanasia , Pappas Anastasios , Iakovidou Ioanna , Nisiotis Athanasios , Kaldrimidis Philippos

Introduction: Ganglioneuromas are rare, benign tumors of mature ganglion cells, arising from the sympathetic ganglia and 20% are located in the adrenal medulla, representing 1–4% of adrenal incidentalomas. About 1/3 secrete catecholamines but hypertension and other adrenergic symptoms are rare. About 60% are depicted with 131I- MIBG.Case report: A 72-year-old man was admitted for a left adrenal tumor 9×8 cm, incidentally found in abd...

ea0016p300 | Endocrine tumours | ECE2008

Proteomic profile of GH-secreting versus non-functioning pituitary tumors

Verpelli Chiara , Mantovani Giovanna , Locatelli Marco , Bello Lorenzo , Peccoz Paolo Beck , Spada Anna , Sala Claudio , Lania Andrea

GH-secreting and non-functioning pituitary tumors are clinically distinct, usually benign but potentially locally aggressive lesions originating from the replication of a single mutated pituitary cell. As for the underlying genetic and epigenetic alterations, also the patterns of activation of specific signaling pathways as well as the prognostic molecular factors leading to local invasiveness are, to date, largely unknown. In this study, we used two-dimensional electrophoresi...

ea0016p301 | Endocrine tumours | ECE2008

Genetic testing of RET protooncogene in multiple endocrine neoplasie type 2 and medullary thyroid carcinoma

Grosz Ute S , Drexler Hjordis H S , Fleischer Sandra , Schulte Heinrich M

Introduction: Multiple endocrine neoplasia type 2 (MEN2) and medullary thyroid carcinoma (MTC) are autosomal-dominant inherited diseases caused by germline mutations within the RET protooncogene. Until now, genetic testing for mutations of exon 10, 11, 13, 14, 15 and 16 was recommended for these patients (familial and sporadic cases) to identify disease-causing mutations. Because of a strong genotype–phenotype correlation in these diseases, early genetic testing of...

ea0016p302 | Endocrine tumours | ECE2008

Impact of the use of recombinant TSH stimulated thyroglobulin measurement on health related quality of life in patients in follow-up for differentiated thyroid carcinoma (DTC)

Persoon ACM , Sluiter WJ , Jager PL , Ranchor AV , Wolffenbuttel BHR , Links TP

Introduction: Thyroglobulin (Tg) measurement is the cornerstone in the follow-up of DTC. Sensitivity can be optimised by measuring recombinant TSH stimulated Tg (rhTSH-Tg). Higher sensitivity results in more Tg positive patients who need imaging and considerable patient burden. We assessed the impact of rhTSH-Tg measurement on health related quality of life (HRQOL).Methods: In 121 patients in follow-up for DTC, Tg during thyroid hormone suppression thera...

ea0016p303 | Endocrine tumours | ECE2008

Cushings' syndrome due to ectopic ACTH secretion: four cases

Yarman Sema , Mert Meral , Salman Serpil , Tanakol Refik , Boztepe Harika , Alagol Faruk

Ectopic ACTH syndrome (EAS) occurs in around 10% of all cases with ACTH-dependent hypercortisolism. The mean age of clinical presentation varies from 45 to 50 years, and most of them caused by intrathoracic neoplasm, and recognition of the disorder may be delay. Subsequently, it may be difficult to locate the ACTH source and manage the patients’ hypercortisolism. We present our patients with EAS from 2000 to 2007. Four patients, aged 15–43 years, two females and two ...

ea0016p304 | Endocrine tumours | ECE2008

Inhibition of intracellular signaling pathways and induction of cell cycle arrest by the multi-kinase inhibitor sorafenib in thyroid carcinoma cells

Broecker-Preuss Martina , Redmann Anja , Britten Martin , Mann Klaus

Objective: Therapeutic options for patients with dedifferentiated thyroid carcinoma are rare and alternative treatment strategies are needed. Among the most promising new agents for these patients are protein kinase inhibitors like the BRAF- and multi-targeted kinase inhibitor sorafenib. We have already shown that sorafenib inhibits growth of dedifferentiated thyroid carcinoma cell lines with and without BRAF mutation. The purpose of this study was to analyze the molecular eff...

ea0016p305 | Endocrine tumours | ECE2008

A rare case of hiperandrogenism bilateral Leydig cell tumor of the ovary

Marcelino Mafalda , Nobre Ema Lacerda , Conceicao Joao , Carvalho Arlindo , Horta Ana Paula , Jacome de Castro Joao

Background: The androgen-secreting tumors constitute fewer than 1% of ovarian tumors. Leydig cell tumor is one of the most common of this type of lesion and it is usually benign, small and unilateral.Case report: A 67-year-old women was referred to the Endocrine clinic due to hirsutism (score 22 Ferriman–Gallwey) and male type alopecia with 3 years of evolution and progressive worsening. Biochemically she had high levels of serum testosterone –...

ea0016p306 | Endocrine tumours | ECE2008

Distinct catecholamine phenotypes in hereditary pheochromocytoma

Eisenhofer Graeme , Lenders Jacques WM , Mannelli Massimo M , Bornstein Stefan R , Pacak Karel

This study examined whether different forms of hereditary pheochromocytoma are characterized by different catecholamine phenotypes and whether this is reflected by differences in plasma concentrations of normetanephrine, metanephrine and methoxytyramine – the respective O-methylated metabolites of norepinephrine, epinephrine and dopamine. Subjects included 154 patients with hereditary pheochromocytoma, 72 with tumors associated with von Hippel–Lindau (VHL) syndrome, ...

ea0016p307 | Endocrine tumours | ECE2008

VDR gene polymorphisms in patients with differentiated thyroid cancer

Penna-Martinez Marissa , Stern Julienne , Kahles Heinrich , Reusch Jurgen , Hinsch Nora , Hansmann Martin-Leo , Selkinski Ivan , Grunwald Frank , Wahl Robert , Holzer Katharina , Ramos-Lopez Elizabeth , Badenhoop Klaus

Background/aim: Vitamin D receptor (VDR) expression has been shown to be upregulated in several tumors and is thought to represent an important endogenous response to tumor progression. Therefore, in order to evaluate the role of VDR-gene and of the 25(OH)-Vitamin D3 in thyroid cancer we analysed four polymorphisms in patients with thyroid cancer and healthy controls.Patients and methods: Patients (n=136; 84 females and 52 males) with d...

ea0016p308 | Endocrine tumours | ECE2008

ERCC1 expression in adrenocortical carcinoma: relationship with baseline characteristics and response to platinum-based chemotherapy

Ronchi Cristina L , Sbiera Silviu , Adams Patrick , Kraus Luthgard , Linss Heidi , Wortmann Sebastian , Willenberg Holger , Allolio Bruno , Fassnacth Martin

Adrenocortical carcinoma (ACC) is a malignant tumor with poor prognosis and no established therapy in advanced stage. Cisplatin is the most frequently used cytotoxic drug, but even combined with doxorubicin and etoposid, the response rate is <50%. Recently, it has been demonstrated that the excision repair cross complementing group 1 (ERCC1) plays a relevant role in the DNA repairing process, particularly in the correction of platinum-induced DNA adducts. Accordingly, ERCC...

ea0016p309 | Endocrine tumours | ECE2008

Leptin exerts apoptotic effects and regulates androgen receptors in human prostate cancer cells

Samuel Sigal , Weiss-Messer Esther , Barkey Ronnie J

Introduction: Prostate cancer (PCa) progression is known to depend on various hormones and growth factors, but their role and underlying molecular mechanisms remain poorly understood. We recently presented preliminary findings indicating that leptin causes a greater level of activation of the JAK2/STAT3 and MAPK (ERK1/2) pathways, as well as transactivation of HER2, in androgen-sensitive LNCaP cells than in androgen-insensitive PC3 and DU145 human PCa cell lines. We and others...

ea0016p310 | Endocrine tumours | ECE2008

Long-term survival after surgical treatment in patients of adrenocortical carcinoma

Britvin Timur , Bogatyrev Oleg , Pirogov Denis , Gadzyra Alexander , Kalinin Arian

Adrenocortical carcinoma is a rare tumor characterized aggressive growth and poor prognosis. This tumor requires the complex way of treatment, where basic method is surgery.The aim of this study was to evaluate the long-term outcome and role of surgery in patients with adrenocortical carcinoma.Methods: From 1998 to 2007, 44 patients were operated for adrenocortical carcinoma (14 cortisol-secreting tumors, 1 virilizing tumor and 29 ...

ea0016p311 | Endocrine tumours | ECE2008

Parathyroid-hormone related- Peptide and PTHrP receptor type 1 are expressed in human adrenocortical carcinoma and regulate cell proliferation and apoptosis in H295R an adrenocortical-derived cell line

Rizk-Rabin Marthe , Assie Guillaume , Fernande Fernande , Perlemoine Karine , Hamzaoui Hinda , Tissier Frederique , Lieberherr Michele , Bertagna Xavier , Bertherat Jeroome , Bouizar Zhor

Adrenocortical tumor (ACT) is a rare, heterogeneous malignancy whose pathogenesis is unclear. The oncoprotein PTHrP, found in many common tumors, can regulate their growth in an autocrine/paracrine fashion through the receptor PTH-R1. Little is known about the role of PTHrP in ACT. We monitored the synthesis of PTHrP and PTH-R1 in a series of 25 ACT: 12 adrenocortical carcinoma (ACC), 13 adrenocortical adenoma (ACA), and investigated the effects of PTHrP (1–34) on H295R c...

ea0016p312 | Endocrine tumours | ECE2008

Prognostic factors in localized adrenocortical carcinoma (ACC) after complete resection

Obracay Jens , Fassnacht Martin , Saeger Wolfgang , Johanssen Sarah , Marcus Quinkler , Linden Tobias , Ann-Kathrin Koschker , Sebastian Wortmann , Morcos Michael , Willenberg Holger , Langer Peter , Behrend Matthias , Klose Silke , Reisch Nicole , Brauckhoff Michael , Petersenn Stephan , Bucsky Peter , Oelkers Wolfgang , Maeder Uwe , Hahner Stefanie , Allolio Bruno

Even after complete resection, patients with ACC have a high risk of relapse, and adjuvant treatment with mitotane is frequently recommended. Although mitotane has significant efficacy in this setting, it is associated with a wide range of side effects. As survival is highly variable, prognostic factors are of great interest to better guide adjuvant therapy after radical resection.We analyzed clinical and histopathological data of patients without distan...

ea0016p313 | Endocrine tumours | ECE2008

Multi-center, observational study on Sandostatin LAR treatment patients with acromegaly in Poland: preliminary report

Zgliczynski Wojciech , Zdunowski Piotr , Sowinski Jerzy , Kos-Kudla Beata , Bolanowski Marek , Bednarek-Tupikowska Grazyna , Kunert-Radek Jolanta , Mucha Slawomir

Aim: The aim of multi-center, observational study was to assess the outcome of the treatment with somatostatin analogue octreotide-LAR (O-LAR) patients with acromegaly.Material: Material consisted of 360 patients (60% women) aged 18–84 years (mean 45.5; S.D.±12.9) with active acromegaly. Prior to the inclusion 172 patients underwent unsuccessful neurosurgery.Methods: Observation was planned for 1 year. O-LA...

ea0016p314 | Endocrine tumours | ECE2008

A study on investigations for localizing insulinoma

Ul-Haq Mazhar , Vora Jiten P

We studied the practice of investigations for diagnosing and localising insulinoma in our hospital. We looked at the number of investigations performed before exact localisation of insulinoma.We identified 5 cases that were confirmed on clinical, biochemical or histopathological bases and were recorded on the histopathology and IT database as ‘insulinoma’ from 1986 to 2004. We looked retrospectively at the case notes of these cases.<p class...

ea0016p315 | Endocrine tumours | ECE2008

Epidemiology of pituitary tumours in Iceland 1955--2007: a Nationwide Study

Baldvinsdottir Tinna , Jonasson Jon G , Thorsson Arni V , Bragadottir Asta , Sigurdsson Gunnar , Benediktsson Rafn

Pituitary tumours may be more prevalent than previously appreciated. Although this is thought to relate mostly to greater utilisation of imaging techniques in recent years, evidence suggests that the increased prevalence also applies to clinically important tumours.We have created a nationwide registry of pituitary tumours occurring in Iceland for the last 55 years. We have examined medical, surgical, pathology and imaging records at all hospitals as wel...

ea0016p316 | Endocrine tumours | ECE2008

Effects of pasireotide on bronchial carcinoids in primary culture

Zatelli Maria Chiara , Tagliati Federico , Luchin Andrea , Bondanelli Marta , Rea Federico , degli Uberti Ettore

Bronchopulmonary endocrine tumors represent 25–30% of lung neoplasms. Surgery provides good survival for differentiated tumors (typical and atypical carcinoids), but is not useful for aggressive poorly differentiated forms. Somatostatin (SRIF) analogs can be used as medical therapy, prolonging patient survival. However, the compounds employed so far did not display antiproliferative effects. Recently, a new stable SRIF analog, pasireotide (SOM230), which activates SSTR1, ...

ea0016p317 | Endocrine tumours | ECE2008

The prognostic factors in adrenocortical carcinoma

Przybylik-Mazurek Elwira , Hubalewska-Dydejczyk Alicja , Kuzniarz Sylwia , Lubaszewska Katarzyna , Huszno Bohdan

Adrenocortical carcinoma (ACC) is a rare neoplasm with poor prognosis. Patients present signs of hormone excess: virilisation, Cushing’s syndrome or only enlarged abdominal mass. Incidentally ACC can be also detected in the ultrasonography. Some of ‘non-hypersecretory’ ACCs can produce non-bioactive hormones steroid precursors or not very big amount of them and sometimes patients present subclinical Cushing’s symptoms. Surgery and adjuvant radiotherapy and ...

ea0016p318 | Endocrine tumours | ECE2008

Cushing's syndrome due to a pigmented nodular adrenocortical disease and a acromegaly corresponding to a Carney complex (CNC)

Sanabria Concha , Perez-Ferre Natalia , Diaz Jose Angel

The CNC is a dominantly syndrome, characterized by spotty skin pigmentation, endocrine overactivity and myxomas (Carney & Young 1992), associated with lentigines and blue naevi; the disease links to 17q22-24, and its mutations have been identified in the genes PRKAR1α. The most common endocrine manifestations affect two o more endocrine glands, including acromegaly, thyroid and testicular tumours and ACTH-independent Cushing’s syndrome due to primary pigmented no...

ea0016p319 | Endocrine tumours | ECE2008

Self-observations of total life situation in patients with Acromegalia

Jensen Jonna Gintberg

Background: Acromegalia is an infrequent chronic disease requiring lifelong control. A total of about 350 Danish patients have acromegalia. It is difficult for this patient group to find information on coping strategies in fellow patients.There are numerous international quantitative studies on the symptoms in acromegalia patients; no qualitative studies on self-observations as a way to exchange experiences and optimize nursing in connection with hospita...

ea0016p320 | Endocrine tumours | ECE2008

Mitotane induces a concentration-dependent impairment of platelet aggregation in patients with adrenocortical carcinoma

Hahner Stefanie , Eigenthaler Martin , Savvoukidis Theodoros , Johanssen Sarah , Wortmann Sebastian , Koschker Ann-Cathrin , Allolio Bruno , Fassnacht Martin

Standard treatment of adrenocortical carcinoma (ACC) comprises adrenolytic therapy with mitotane. Prolongation of bleeding time has previously been observed based on a series of 7 patients (Haak et al. 1991). As patients with ACC frequently undergo surgery for local recurrence or metastases, we have studied the effect of mitotane on coagulation in 44 patients with ACC before and/or during treatment with mitotane (total sample size n=62).Pla...

ea0016p321 | Endocrine tumours | ECE2008

One hundred minimally invasive parathyroidectomies without intraoperative localisation and PTH monitoring

Farooq Muhammad Suleman , Saharay Mrinal

Background: Minimally invasive parathyroidectomy (MIP) for primary hyperparathyroidism is routinely performed in many centres. Various preoperative and intraoperative localisation techniques are used along with intraoperative PTH monitoring (IOPTH).Results: We report the results of 110 consecutive patients presenting to our unit with a diagnosis of primary hyperparathyroidism from January 2004 until November 2007. All patients had sestamibi scintigraphy ...

ea0016p322 | Endocrine tumours | ECE2008

Effect of Ginkgo biloba extract supplementation on genotoxic damage after thyroid remnant ablation by 131I

Dardano Angela , Ballardin Michela , Traino Claudio , Caraccio Nadia , Colato Chiara , Barale Roberto , Mariani Giuliano , Ferdeghini Marco , Monzani Fabio

Background: Radioiodine (131I) therapy is performed in patients with differentiated thyroid cancer (DTC), either for thyroid remnant ablation or treating distant metastasis. Although 131I therapy is generally considered safe, a genotoxic damage has been demonstrated both in vivo and in vitro.Aim: To evaluate the possible effect of Ginkgo biloba extract (EGb 761) supplementation on the time-course (up to 1...

ea0016p323 | Endocrine tumours | ECE2008

Neuroendocrine tumours (NETs): one centre experience

Hubalewska-Dydejczyk Alicja , Trofimiuk Malgorzata , Sowa-Staszczak Anna , Szybinski Piotr , Kulig Jan , Pach Dorota , Gilis-Januszewska Aleksandra , Huszno Bohdan

Background: NETs are rare tumours arising from dispersed neuroendocrine system. Nevertheless, their estimated prevalence increased lately, mostly due to progress in imaging, biochemical and histopathological diagnostics.The aim of the study was to present the characteristics of the NETs patient surveyed in our Endocrinology Department since 2000.Material and methods: One hundred and fifty-eight patients (males – 42.4%, females...

ea0016p324 | Endocrine tumours | ECE2008

Abnormalities in glucose tolerance in acromegalic patients

Stelmachowska-Banas Maria , Zdunowski Piotr , Zgliczynski Wojciech

Background: Acromegaly is characterized by disabling symptoms and relevant comorbidities. Insulin resistance, leading to glucose intolerance is one of the most important contributory factor to the cardiovascular mortality in acromegaly.Aim: To assess the impairments of glucose homeostasis in acromegalic patients and find association between activity of the disease and the severity of glucose intolerance.Patients and methods: In thi...

ea0016p325 | Endocrine tumours | ECE2008

Mutations in the ret proto-oncogene in Romanian patients with multiple endocrine neoplasia type 2

Paun Diana , Duta Carmen , Mohora Maria , Dogaru Cristian , Dumitrache Constantin

Multiple endocrine neoplasia (MEN) is characterized by the occurrence of tumors involving two or more endocrine glands; MEN 2 is defined by medullary thyroid carcinoma in association with phaeochromocytoma and appears in several clinical variants, which may be inherited as autosomal dominant syndromes. Mutational analysis of RET protooncogene has been use in the diagnosis and management of patients and families with MEN 2 variants.Aim: In this study, we ...

ea0016p326 | Endocrine tumours | ECE2008

Is the early postoperative hormonal assessment a helpful predictor of the long term remission in secreting pituitary adenomas after transsphenoidal adenomectomy?

Witek Przemyslaw , Zgliczynski Wojciech , Zdunowski Piotr , Stelmachowska-Banas Maria , Zielinski Grzegorz

Introduction: Transsphenoidal adenomectomy is the treatment of choice in secreting pituitary adenomas with the symptoms of Cushing’s disease, acromegaly as well as few cases of Prolactinoma, which – due to resistance to pharmacological treatment – require surgery. The efficacy of selective adenomectomy ranges from 60 to 95%. It is the highest in patients with well-visualized by MRI microadenomas, lower in macroadenomas and the lowest when the surgery was ...

ea0016p327 | Endocrine tumours | ECE2008

Prognostic value of 100 mcg s.c. octreotide test (SHort Octreotide Test – SHOT) for prediction of medical treatment outcome in patients with acromegaly

Zdunowski Piotr , Zgliczynki Wojciech

Background: Neurosurgery is treatment of choice in patients with GH-secreting pituitary adenoma. However, in about 50% of cases surgery is ineffective or contraindicated. Long-acting somatostatin analogues are possible, although expensive therapeutic alternative. Generally, 60–70% of patients with active disease responds to such medical treatment.Aim: Aim of this study was to identify factors influencing medical treatment outcome and to determine if...

ea0016p328 | Endocrine tumours | ECE2008

Cushing syndrome with an atypical evolution

Lecomte Pierre , Pierre Peggy , Castric Marie , Chabrolle Christine , de Calan Loic , Guyetant Serge

A 72-year-old depressed woman complained about fatigue, weight gain (20 kilos/2 years), severe and recent hypertension, easy bruises, and venous insufficiency. Whereas she was treated with six antihypertensive drugs, she had hypokaliemia (2.6 mM). Cardiac ultrasounds showed myocardial hypertrophy. A Cushing syndrome was suspected. UFC was increased (400 mM/d), cortisol cycle disrupted and a standard DXM test showed no cortisol suppression (775 nM/l). ACTH levels were consisten...

ea0016p329 | Endocrine tumours | ECE2008

Parathyroid carcinoma treated with cinacalcet: a preliminary report

Castric Marie , Pierre Peggy , Magnant Julie , Chabrolle Christine , Mor Caroline , Orain Isabelle , Lecomte Pierre

A 61-year-old man was hospitalized for paresthesias and renal insufficiency (creatinine clearance 24 ml/min) with proteinuria. Type 2 diabetes was diagnosed in 2003 and hypertension in 2007. Hypercalcemia (4.6 mM) with normal phosphorus level 1.23 mM was discovered with very high PTH levels (863 ng/l; N<78). MIBI scintigraphy localized a right inferior parathyroid tumour. No obvious sign favouring MEN1 was observed and no familial case was reported. After a short pa...

ea0016p330 | Endocrine tumours | ECE2008

Ovarian serous and mucinous tumors of low malignant potential: patterns of stromal invasion

Costi Simona , Dema Alis , Golu Ioana , Cornianu Marioara , Taban Sorina , Muresan Anca , Lazar Elena

Purpose: The purpose of this study was to evaluate the histologic spectrum of stromal–epithelial patterns of invasion in ovarian serous and mucinous tumors of low malignant potential.Materials and methods: We retrospectively analyzed 31 cases of borderline ovarian serous and mucinous tumors diagnosed of the Pathology Department of County Hospital Timisoara in a period of 5 years, between 2003 and 2007. Epithelial architecture, the patterns of stroma...

ea0016p331 | Endocrine tumours | ECE2008

Novel MEN1 germline mutations and clinical features in Greek patients with multiple endocrine neoplasia type 1

Peppa Melpomeni , Pikounis Vasilios , Kamakari Smaragda , Peros George , Economopoulos Theofanis , Raptis Sotirios A , Hadjidakis Dimitrios

Introduction: Multiple endocrine neoplasia type 1 (MEN1) is an autosomal dominant hereditary disorder, associated with mutations of the MEN1 gene, characterised by the combined occurrence of tumours of the parathyroid glands, the pancreatic islet cells and the anterior pituitary.Aim: To identify MEN1 gene mutations and characterize clinical manifestations in Greek patients with MEN1.Patients and methods: We studied 4 ...

ea0016p332 | Endocrine tumours | ECE2008

Ghrelin and its receptor are present in an ectopic ACTH lung neuroendocrine tumour causing Cushing's syndrome: potential pathophysiological implications

Quintero Ana , Martinez-Fuentes Antonio J , Gahete Manuel D , Vazquez-Martinez Rafael , Gutierrez-Alcantara Carmen , Jimenez-Reina Luis , Benito-Lopez Pedro , Malagon Maria M , Castano Justo P

Ghrelin is a 28-aa peptide originally isolated from stomach but present also in many tissues, including hypothalamus and pituitary, where it stimulates growth hormone (GH) release through the ghrelin/GH secretagogue receptor (GHS-R). Ghrelin also increases food intake and adiposity and could play a key integrative role in the endocrine–metabolic interface. Although its primary pituitary cell target are somatotropes, ghrelin also modulates other pituitary cell types, speci...

ea0016p333 | Endocrine tumours | ECE2008

Deficits in the clinical management of patients with adrenocortical carcinoma in Germany

Johanssen Sarah , Koschker Ann-Cathrin , Hahner Stefanie , Quinkler Marcus , Saeger Wolfgang , Morcos Michael , Willenberg Holger , Langer Peter , Klose Silke , Reisch Nicole , Beuschlein Felix , Brauckhoff Michael , Fottner Christian , Wortmann Sebastian , Oelkers Wolfgang , Maeder Uwe , Fassnacht Martin , Allolio Bruno

Adrenocortical carcinoma (ACC) is a rare disease with poor prognosis. Accordingly, in many cases, the attending doctors have no previous experience with the disease. The aim of our study was to evaluate the quality of care in a large number of patients with ACC in Germany. Data from 263 adult patients of the German ACC registry were analyzed with regard to the following parameters: time to diagnosis, hormonal assessment, imaging, histopathological documentation, follow-up. The...

ea0016p334 | Endocrine tumours | ECE2008

The cAMP analog 8CL-cAMP enhances PKA RIIb, triggers apoptosis and blocks cells at S and G2 cell cycle phases after 4 days of treatment in the human adrenocortical cells H295R.

Bouizar Zhor , Berterat Jerome , Rizk-Rabin Marthe

Various alterations of the cyclic AMP (cAMP) signalling cascade has been observed in adrenocortical tumors. Changes of the PKA regulatory subunits expression in tumors may positively or negatively regulate proliferation. 8CL-cAMP, a site selective cAMP analogue, induces growth inhibition in a variety of human cancer cell lines. The aim of the study was to determine if 8CL-cAMP acts on adrenocortical carcinoma H295R cell growth.We have determined the invo...

ea0016p335 | Endocrine tumours | ECE2008

Hyperprolactinaemia: different clinical expression in childhood

Saranac Ljiljana , Kostic Gordana , Stamenkovic Hristina , Zivanovic Snezana , Slavkovic-Jovanovic Maja

Hyperprolactinaemia is the most common disturbance in the pituitary gland function. Different physiological and patological conditions could influnce prolactin (PRL) secretion. Prolactin secreting tumors (micro and macroprolactinomas) are rare in children and adolescents (estimated incidence is 1 per milion). Functional diversity of prolactin action is responsable for different initial clinical expression of hyperprolactinaemia.We investigated causes of ...

ea0016p336 | Endocrine tumours | ECE2008

G12/13-dependent growth of small cell lung cancer cells in vitro and in vivo

Buch Thomas , Grzelinski Marius , Pinkenburg Olaf , Gold Maike , Aigner Achim , Gudermann Thomas

The malignant phenotype of small cell lung cancer (SCLC) cells critically relies on the autocrine stimulation of G12/13- and Gq/11-coupled neuropeptide receptors. By this means, G12/13- and Gq/11-dependent signalling pathways are constitutively activated in these cells. While Gq/11-dependent signalling has been shown to promote proliferation of SCLC cells, the role of signalling pathways via G12/13 is still elus...

ea0016p337 | Endocrine tumours | ECE2008

High prevalence of pituitary adenomas: a cross-sectional study in the city of Banbury (Oxfordshire)

Fernandez Alberto , Karavitaki Niki , Wass John AH

Background: Pituitary adenomas (PA) are considered very rare conditions, with an estimated prevalence of 25 cases/100 000 inhabitants. However, community-based studies on the prevalence of PA are currently scant.Aim: To ascertain the prevalence of PA and the characteristics of the patients diagnosed with them in a large population of inhabitants.Methods: A survey on the GP surgeries of Banbury (Oxfordshire, UK) covering 89 334 inha...

ea0016p338 | Endocrine tumours | ECE2008

Gastric NETs: new diagnostic and therapeutic approach

Hubalewska-Dydejczyk A , Gilis-Januszewska A , Szybinski P , Sowa-Staszczak A , Klek S , Huszno B , Kulig J

The incidence of GNT appears to be increasing, what can be explained by the increased detection caused by the common use of the endoscopy and the pervasive use of acid suppressive therapy leading to enterochromatofine like cells proliferation. There are numerous new diagnostic/therapeutic GNT methods in use like: EUS, SRS, somatostatin therapy and 90Y/177Lu-DOTA-TATE radiotherapy.Materials and methods: In 1998–2007, 25 patients were diagnosed with t...

ea0016p339 | Endocrine tumours | ECE2008

Fenotypes in patients with Y791F mutation of RET protooncogene

Isailovic Tatjana , Petakov Milan , Macut Djuro , Popovic Bojana , Bozic Ivana , Ognjanovic Sanja , Damjanovic Svetozar

Mutation Y791F of RET protooncogene is a well known mutation so far described in families with FMTC and familial pheochromocytoma in one family. It activates the receptor in a monomeric form. Here we present eight unrelated families with the same mutation but different fenotype expression.Patients and methods: In last 20 years, we analyzed 216 patients with MTC (age range: 3–75 years, 45.0 mean). Genetic testing for mutation in RET pro...

ea0016p340 | Endocrine tumours | ECE2008

Insulin sensitivity in a patient with gastric ghrelinoma

Popovic Bojana , Bozic Ivana , Macut Djuro , Lalic Nebojsa , Jotic Aleksandra , Djuranovic Srdjan , Isailovic Tatjana , Petakov Milan , Damjanovic Svetozar

Ghrelin is a somatotrophic, orexigenic and adipogenic hormone that has an important homeostatic role by linking regulatory systems for growth and energy balance. Ghrelin- secreting neuroendocrine tumors are rare, but we had the opportunity to study a 71 years old female patient with a gastric ghrelinoma. The patient was submitted to gastroenterological examination after having episodes of diarrhea, and small subepithelial polyp (5 mm) was revealed on gastroscopy. Pathohystolog...

ea0016p341 | Endocrine tumours | ECE2008

Association of genetic variants of somatostatin receptor 5 with acromegaly

Klovins Janis , Kapa Ivo , Balcere Inga , Ciganova Darja , Nikitina-Zake Liene , Schioth Helgi , Pirags Valdis

Full gene coding sequence of somatostatin receptor 5 and 2000 bp of upstream region was estimated using direct sequencing in 28 patients with acromegaly and 97 controls. Total 19 polymorphisms were identified (SNP1-19) and possible haplotypes were reconstructed. From all polymorphisms found silent substitution SNP15 was significantly associated with acromegaly as compared with control group (P=0.005). Another substitution SNP17 involves the amino acid change from prolin...

ea0016p342 | Endocrine tumours | ECE2008

Cushing syndrome: cycling around diagnosis

Mota Filipe , Matos Catarina , Afonso Miguel , Almeida Rui , Marques Olinda

Introduction: Cyclic Cushing syndrome (CCS) involves rhythmic fluctuations in ACTH secretion resulting in a cyclic variation of adrenal steroid production.Case presentation: A 37-year-old woman was referred to our Pituitary Clinic in 1999, for evaluation of galactorrhea and irregular menses that had started four months ago. She also complained of depression, facial hirsutism, alopecia and progressive obesity. Blood pressure was normal. Endocrinological e...

ea0016p343 | Endocrine tumours | ECE2008

Raloxifene induces growth arrest and apoptosis in prostate cell lines expressing both ERα and β

Bellastella Giuseppe , Rossi Valentina , Visconti Daniela , Palumbo Vincenzo , Chieffi Paolo , Staibano Stefania , Mascolo Giuseppe , Preziosi Domenico , Bellastella Antonio , Sinisi Antonio Agostino

Raloxifene (RAL) is a selective estrogen receptor (ER) modulator (SERM) proposed for chemoprevention of breast cancer and osteoporosis. SERMs exert agonist–antagonist effects depending on tissue or ERs expressed. RAL induces apoptosis in both androgen-dependent and independent cell lines, suggesting a selective activation of ERβ and prevalent antagonist effect on ERα in prostate cells (PC). In this study we evaluated effects of estradiol (E2) and RAL on epitheli...

ea0016p344 | Endocrine tumours | ECE2008

Pituitary adenomas: the reality of Braga

Mota Filipe , Matos Catarina , Afonso Miguel , Almeida Rui , Marques Olinda

Introduction: There are limited data on the incidence and prevalence of pituitary adenomas (PA). Although classically considered rare, systematic assessments from autopsy and radiological studies have shown a prevalence of 17% in the general population.Objective: We describe the epidemiologic of PA in Braga, the third most populous district of Portugal (860 000 inhabitants, 2673 km2), over the last 20 years.Methods: We r...

ea0016p345 | Endocrine tumours | ECE2008

Deregulated Wnt/β-catenin signaling in breast cancer: the LRP5Δ receptor

Bjorklund Peyman , Svedlund Jessica , Olsson Anna-Karin , Westin Gunnar , AEkerstrom Goran

Background: Aberrant accumulation of cytoplasmic/nuclear β-catenin frequently occurs in breast cancers and breast cancer cell lines. Deregulated expression of Wnt ligands, sFRP1 and Dvl1, but no inactivating or activating mutations in Wnt signaling components have so far been reported. We have recently described an internally truncated LRP5 receptor (LRP5Δ) in 91% of hyperparathyroid tumors, and showed that LRP5Δ was strongly implicated in the aberrant accumulat...

ea0016p346 | Endocrine tumours | ECE2008

p107Rbl1 is an haploinsufficient tumor suppressor in mouse pituitary

Martinez Gloria , Carneiro Carmen , Estravis Miguel , Yeh Nancy , Koff Andrew , Vidal Anxo

The pocket protein family members, pRb, p107 and p130, are negative regulators of the cell cycle. But, while the role of pRB as a tumor suppressor has been extensively established, genetic inactivation of p107 or p130 is uncommon in tumors. Previous studies have shown that p130 cooperates with the CDK inhibitor p27Kip1 restricting proliferation of mouse intermediate lobe pituitary cells. Accordingly double knockout mice show higher incidence of pituitary tumors than single KO ...

ea0016p347 | Endocrine tumours | ECE2008

Immunohistochemical evaluation of ghrelin expression in somatotroph and non-functioning pituitary tumours

Jaskula Magdalena , Wasko Ryszard , Kotwicka Malgorzata , Liebert Wlodzimierz , Sowinski Jerzy

Introduction: Ghrelin is known to strongly stimulate growth hormone release from the pituitary. It was also found to be produced locally in hypothalamus and anterior pituitary and in different types of pituitary adenomas. It’s been suggested that ghrelin synthesized locally in hypothalamo–pituitary area or within pituitary tumours can be an important factor contributing to pituitary tumorogenesis.Aim: The aim of the study was the immunohistoche...