ISSN 1470-3947 (print) | ISSN 1479-6848 (online)

Endocrine Abstracts (2008) 16 P534

Insulin and glucose regulates omentin-1, a novel adipokine: relation to women with the polycyastic ovary synfrome

Bee K Tan1, Raghu Adya1, S Farhatullah1, KC Lewandowski2, Paul O’Hare1, Hendrik Lehnert3 & Harpal S Randeva1

1Endocrinology and Metabolism Group, Warwick Medical School, Clinical Sciences Research Institute, University of Warwick, Coventry, UK; 2Department of Endocrinology and Metabolic Diseases, The Medical University of Lodz and Polish Mother’s Memorial Research Institute, Lodz, Poland; 31st Medical Department, University of Lübeck Medical School, Luebeck, Germany.

Polycystic ovary syndrome (PCOS) is associated with insulin resistance and obesity. Recent studies have shown that plasma omentin-1 levels decrease with obesity. Currently, no data exists on the relative expression and regulation of omentin-1 in adipose tissue (AT) of PCOS women.

Objectives: To assess mRNA and protein levels of omentin-1 in omental (om) AT of PCOS women and matched controls, including circulating omentin-1. Ex vivo and in vivo regulation of AT omentin-1 was also studied.

Research design and methods: Real-time RT-PCR and western blotting were used to assess mRNA and protein expression of omentin-1. Plasma Omentin-1 was measured by ELISA. The effects of D-glucose, insulin, gonadal and adrenal steroids on AT omentin-1 were analysed ex vivo. The in vivo effects of insulin (hyperinsulinemia) on omentin-1 levels were also assessed by a prolonged insulin-glucose infusion.

Results: In addition to decreased plasma omentin-1 levels in PCOS women (P<0.05), compared to controls, there was significantly lower levels of omentin-1 mRNA (P<0.01) and protein (P<0.05) in om AT of PCOS women (P<0.01). Furthermore, in om AT explants, insulin and glucose significantly dose-dependently decreased omentin-1 mRNA expression, protein levels and secretion into conditioned media (P<0.05, P<0.01). Also, hyperinsulinemic induction in healthy subjects significantly reduced plasma omentin-1 levels (P<0.01).

Conclusions: Our novel findings reveal that omentin-1 is down regulated by insulin and glucose. These may in part explain the decreased omentin-1 levels observed in our overweight PCOS women.

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