Searchable abstracts of presentations at key conferences in endocrinology
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12th European Congress of Endocrinology

ea0022p267 | Diabetes | ECE2010

Have the glitazones anything to do with fractures?

Suarez-Lledo Jose

Objective: Thiazolidinedione (glitazone) (GLT) use was reported to increase frequency of fractures by the ADOPT study. Investigation of this discovery is the aim of the present work.Materials and methods: The patients and their clinical histories were examined when each ambulatory patient was personally visited by the author, for 6 months: 108 males and 160 females were seen. The GLT group was compared with the OAD and insulin user group. Moreover, the r...

ea0022p268 | Diabetes | ECE2010

Insulin-induced lipohypertrophy, past, present and future: are we losing the battle?

Mlawa Gideon , Balami Dauda , Deshmukh Sandeep , Croft Maureen , Bodmer Charles , Patel Mayank

Background: Poor glycaemic control with erratic blood glucose levels manifesting as recurrent hyperglycaemia with unpredictable episodes of hypoglycaemia is problem still faced today in daily practice. The causes include poor compliance and failure of oral hypoglycaemic. Despite being on insulin (38% of type 2 diabetes patients who require insulin treatment after 10 years) and type 1 diabetes patients continue to have fluctuating glucose concentration. The poor glycaemic contr...

ea0022p269 | Diabetes | ECE2010

Body mass index, disease duration and rheumatologic presentations in diabetes mellitus, Gorgan (North part of Iran)

Emadi Batool , Aghaie Mehrdad , Bazrafshan Hamidreza , Banikarim Amir , Behnampur Naser

Background: Diabetes mellitus has various characteristics in different organs. Some of the most important signs and symptoms are seen in joints and musculoskeletal system.Objectives: This study was designed to evaluate the effect of body mass index (BMI) on rheumatologic presentations of diabetic patients in Gorgan, Northeast of Iran.Methods: This was descriptive-analytic study which was done on DM patients whom referred to endocri...

ea0022p270 | Diabetes | ECE2010

A case of diabetic ketoacidosis with ECG abnormalities mimicking acute coronary syndrome

Ozkaya Evrim Cakir , Ozbek Mustafa , Bozkurt Nujen Colak , Cakal Erman , Karbek Basak , Delibasi Tuncay

Diabetic ketoacidosis (DKA) seen in 10–70% of patients with type 1 diabetes mellitus (DM1) has a significant risk of mortality. We present a DKA in a patient with DM1 resulted in hypokalemia related ECG abnormalities that the diagnosis needed to be differentiated from acute coronary syndrome. A 18-year-old women without any significant past medical history admitted to hospital with a two day history of fatique, weakness, nausea, vomiting. Patient appeared hipovolemic. Vit...

ea0022p271 | Diabetes | ECE2010

ESE Young Investigator Award

dos Santos Ferreira Marques Milano Felipe , Mori Rosana Cristina Tieko , Okamoto Maristela Mitiko , Machado Ubiratan Fabres

Introduction: GLUT4 protein is the most important isoform of glucose transporters in skeletal muscle. Reduction in its content and/or translocation is related to insulin resistance and type 2 diabetes mellitus. In skeletal muscle cells there is also an ATP-sensitive potassium (KATP) channel with two subunits, sulfonylurea receptor type 2A, ABCC9 (SUR2A) and KIR6.2 (KCNJ11). In pancreatic B cells, this channel triggers insulin secretion in response to the rise in glucose levels...

ea0022p272 | Diabetes | ECE2010

Depression, anxiety, quality of life and sleep in patients with type 1 diabetes mellitus

Anaforoglu Inan , Atasoy Volkan , Algun Ekrem , Kutanis Remzi

Aim: The aim of this study was to investigate the quality of life, depression, anxiety and quality of sleepiness and to determine the variables of illness and sociodemographic features among type 1 diabetic patients.Materials and methods: SF-36 quality of life (QOL) Scale, beck depression inventory, state-trait anxiety inventory form and epworth sleepiness scale have been used to evaluate 58 diabetic patients and 58 control subject (31.9±8.5 vs 31.8...

ea0022p273 | Diabetes | ECE2010

The effect of diabetes treatment on non alcoholic fatty liver disorder

Ghareh Sahar , mousavi Zohreh , Rokni Haleh

Nonalcoholic fatty liver disorder is common among diabetics. Few studies have shown that there is association between type of diabetes treatment and prevalence of NAFLD.Materials and methods: The prevalence of NAFLD has been evaluated in 236 diabetic patients and the association between type of diabetes treatment and NAFLD was surveyed.Results: Patients of 63.3% were female. Average age was 50.83±11.21 years. Average weight wa...

ea0022p274 | Diabetes | ECE2010

Insulin resistance in smokers and non-smokers in young healthy population

Acar Emel , Aydin Yusuf , Onder Elif , Cinemre Hakan , Kir Seher , Coskun Hulya , Kudas Ozlem , Turgut Mehmet

Introduction: Insulin resistance is the decrease sensitivity of tissues in the body to insulin effect. Obesity, advanced age, sedentary life and smoking are the factors leading to an increase in insulin resistance. In our study, we aimed to evaluate the relationship between the insulin resistance and smoking in individuals without advanced age, obesity, high blood glucose levels and hypertension.Materials and methods: We included in this study 100 patien...

ea0022p275 | Diabetes | ECE2010

Benefit of switching from rosiglitazone to pioglitazone on cardiovascular markers in routine clinical practice

Triay Jessica , Raghavan Rajeev , Day Andrew , Singhal Parag

Background and aims: Thiazolidinediones have been extensively used as second line agents in the management of type 2 diabetes. A small increased incidence of ischaemic heart disease was suggested by a meta-analysis of studies using Rosiglitazone. Similar meta-analysis of Pioglitazone did not show adverse cardiovascular outcomes. Our aim was to objectively evaluate the changes in glycaemic and lipid control in a controlled conversion from Rosiglitazone to Pioglitazone.<p cl...

ea0022p276 | Diabetes | ECE2010

Advanced glycation end products (AGEs) and high glucose in endothelial cells: gene expression analysis

Zitman-Gal Tali , Green Janice , Pasmanik-Chor Metsada , Oron-Karni Varda , Bernheim Jacques

Background: Elevation of blood and tissue concentrations of glucose and advanced glycation end products (AGEs) may play a relevant role in the occurrence of atherosclerosis, arteriosclerosis and diabetic complications. To evaluate the impact of elevated extracellular AGEs and different glucose concentrations we studied the gene expressions and the underlying pathways which may lead to endothelial inflammation.Methods: Fresh human umbilical vein cord endo...

ea0022p277 | Diabetes | ECE2010

Comparison of lymphomononuclear cell energy metabolism between healthy, impaired glucose intolerance and type 2 diabetes mellitus patients

Ozsari Levent , Karadurmus Nuri , Sahin Mustafa , Uckaya Gokhan , Ural Ali Ugur , Musabak Ugur , Kutlu Mustafa

Objective: Diabetes mellitus (DM) is a complex disease that affects many systems. The most important cells of the immune system are lymphomononuclear (LMN) cells. Here, we aimed to evaluate the energy metabolism of LMN cells in patients with diabetes and impaired glucose tolerance.Methods: We measured LMN cell energy metabolism in patients with type 2 diabetes mellitus, impaired glucose tolerance (IGT) and healthy subjects. Cells were freshly isolated fr...

ea0022p278 | Diabetes | ECE2010

Assessment of the therapeutic effectiveness of gliclazide MR after short-term application

Milenkovic-Vulovic Koviljka , Micic Dragan , Polovina Snezana

The research included 20 patients of average age 59±8.5 years (ranging from 45 to 75 years of age), of whom there were 8 male patients (40%) and 12 female patients (60%), with average diabetes duration of 4.8±3.16 years. In this group HbA1c levels ranged from 6.6% to 9.4%, while the average BMI level was 30±3.39 kg/m2. Prior to the introduction of gliclazide MR with the daily dosage of 30 mg, all the patients had been treated with metformin with the d...

ea0022p279 | Diabetes | ECE2010

Effectiveness of patterns of intensification of premixed insulin analogue therapy

Kalra Sanjay , Kalra Bharti , Unnikrishnan A G , Agrawal Navneet

This study was designed as a retrospective multicentric study to assess the efficacy of various methods of effective intensification and optimization of premixed insulin analogue therapy. 200 consecutive OPD charts of patients poorly controlled on premixed insulin twice daily were reviewed for changes advised by the treating physician. Data was assessed for cues, methods and efficacy of intensification of therapy.A casual blood glucose value >250 mg%...

ea0022p280 | Diabetes | ECE2010

Knowledge, attitudes and practices of type 1 diabetes patients regarding complementary and alternative medicine (CAM)

Kalra Sanjay , Kumar Satish , Kalra Bharti , Agrawal Navneet , Sharma Amit , Prusty Vinay

This multicentric, observational, non interventional study of patients with type 1 diabetes observed their knowledge, attitudes and practices regarding complementary and alternative medicine (CAM), including nutraceuticals. One hundred and thirty-five subjects with type 1 diabetes, aged <20 years, and their parents were surveyed using a structured pre-tested questionnaire.The cohort included 65.92% boys, 37.04% rural dwellers and 31.85% patients with...

ea0022p281 | Diabetes | ECE2010

The frequency of depression estimation among patients with diabetes mellitus

Navmenova Yana , Mokhort Tatiana

One of the most frequent psychological disturbances at patients with diabetes mellitus are depressions. According to the literature, the prevalence of depressions at patients with diabetes mellitus are ranging widely from 3.8 to 60.7%. This differences depend on diabetic population and the method of depression estimation.Objectives: To estimate the frequency of depression in patients with type 1 and type 2 diabetes mellitus (T1DM and T2DM)).<p class=...

ea0022p282 | Diabetes | ECE2010

The Gly972Arg polymorphism in the insulin receptor substrate-1 gene (IRS1) and the impact on type 2 diabetes mellitus

Katalinic Darko , Nikolac Nora , Zjacic-Rotkvic Vanja , Topic Elizabeta , Solter Miljenko , Plestina Stjepko

Background: Genetics may play an important role in type 2 diabetes (T2D). In the last 20 years, there has been a surge in the number of genetic studies in attempts to identify some of the underlying risk factors. Mutations in a wide variety of genes contribute to the deregulation of glucose homeostasis, and seem to confer the risk for developing T2D. Insulin receptor substrate-1 (IRS1) is a substrate of the insulin receptor tyrosine kinase and appears to have a control role in...

ea0022p283 | Diabetes | ECE2010

Impact of the SUR1 16-3 C/T and INSR His 1085 C/T genetic variability on type 2 diabetes mellitus development

Katalinic Darko , Nikolac Nora , Zjacic-Rotkvic Vanja , Topic Elizabeta , Solter Miljenko , Plestina Stjepko

Background: Type 2 diabetes (T2D), which is multifactorial, inherited and progressive chronic disorder, is characterised by hyperglycemia due to defects in insulin secretion and action. The sulfonylurea receptor (SUR1) and the insulin receptor (INSR) are critical elements in insulin-signalling pathways, and mutations in the SUR1 and INSR genes have been reported to have a role in determining susceptibility to T2D.The aim was to study whether the -3 C/T p...

ea0022p284 | Diabetes | ECE2010

Left ventricular hypertrophy and its relation with inflammation in type 2 diabetic patients

Nelaj Ergita , Gjata Margarita , Sadiku Edite , Collaku Ledio , Tase Mihal

Introduction: The association between diabetes and adverse cardiovascular outcome may be partially explained by the strong independent association of type 2 diabetes with cardiovascular target organ damage, such as left ventricular hypertrophy (LVH), a well-known predictor of cardiovascular events independent of coronary artery disease. The aim of the present study is to evaluate the relation of LVH to fibrinogen and C-reactive protein (CRP) as markers of inflammation and susc...

ea0022p285 | Diabetes | ECE2010

Abstract withdrawn....

ea0022p286 | Diabetes | ECE2010

It's time to put the ‘age' and the ‘face' on HbA1c in elderly patients with diabetes mellitus

Mlawa Gideon , Alegbeleye James , D'Esouza Raymond

Introduction: Older patients with diabetes mellitus often have concomitant comorbidities and physical disabilities which may lead to poor quality of life. The present diabetic guidelines available to clinicians in their practice suggest aggressive and meticulous diabetic control for target HbA1c (<7%). This is aimed at delaying or preventing complications. Such practice may predipose majority of older patients to experience recurrent hypoglycaemic epidodes and therefore co...

ea0022p287 | Diabetes | ECE2010

Depression, anxiety, quality of life and sleepiness in family members of type 2 diabetic patients

Anaforoglu Inan , Ramazanogullari Ibrahim , Kutanis Remzi , Algun Ekrem

Aim: The aim of this study was to investigate the quality of life, depression, anxiety and quality of sleepiness and to determine the variables of illness and sociodemographic features among family members of type 2 diabetic patients.Materials and methods: SF-36 quality of life (QOL) scale, beck depression inventory (BDI), state-trait anxiety inventory form (STAI TX-I, STAI TX-II) and epworth sleepiness scale (ESS) have been used to evaluate first degree...

ea0022p288 | Diabetes | ECE2010

Comparative and metabolic study of glycosylated hemoglobin, magnesium, nitrogenous metabolites, and lipid profile in diabetics and non-diabetics patients

Mehrotra Vinit , Awasthi G

Diabetes is the major health care problem in developing countries like India. According to WHO survey total number of diabetics patients in India alone is 3.2 cores with fastest growing population of diabetics. This is not a single disease, rather a group of disorders associated with rearrangement in carbohydrate, protein and fat metabolism.Diabetes is characterized by the elevation of blood glucose level resulting from defects in insulin secretion or ac...

ea0022p289 | Diabetes | ECE2010

Association of diabetes mellitus with thalassaemia and sickle cell disease

Thisiadou Katerina , Karamouzis Ioannis , Arampatzi Stella , Tomos Kostantinos , Michailidou Despoina

Background: The majority of complications in transfusion dependent thallasemic and with sickle cell anemia patients are due to iron overload. This is responsible for the beginning of haemosiderosis, clinical symptoms and dysfunction of important systems, specially after the 2nd decade of life. The increase of the iron accumulation is caused by hemolysis, high iron absorbance and mainly by transfusion treatment. The appearance of diabetes mellitus depends on the significance an...

ea0022p290 | Diabetes | ECE2010

Response of interleukin-6 during euglycaemic and hyperglycaemic exercise in patients with type 1 diabetes mellitus

Jenni Stefan , Wueest Stephan , Konrad Daniel , Stettler Christoph

Background: Secretion of IL-6 during exercise is hypothesised to be regulated according to substrate availability within the working muscle. We have previously shown that energy supply in type 1 diabetes during aerobic exercise in hyperglycaemia strongly relies on carbohydrate oxidation whereas a physiological shift from carbohydrate to lipid oxidation occurs in euglycaemia. The present study investigated whether IL-6 response to exercise is different in euglycaemia compared w...

ea0022p291 | Diabetes | ECE2010

Coagulation inhibitors in type 2 diabetes mellitus

Thisiadou Katerina , Karamouzis Ioannis , Arampatzi Stella , Lazaridou Despoina

Background: Diabetes mellitus (DM) is related to hypercoagulability.The predisposition for thrombosis disorders is caused by increased accumulation of platelets, by high concentrations of prothrombotic factors and by the reduced concentration and activity of the inhibitor factors. Abnormalities of coagulation inhibitors have been reported in many studies on diabetes over the last years, but unfortunately, the results have often appeared contradictory.</p...

ea0022p292 | Diabetes | ECE2010

Linagliptin, a potent and selective DPP-4 inhibitor, does not prolong the QT interval when given in therapeutic and 20-fold supratherapeutic doses

Ring Arne , Graefe-Mody Ulrike , Port Andreas , Revollo Ivette , Walter Beate , Woerle Hans-Juergen , Iovino Mario , Dugi Klaus

Background: Cardiovascular safety is important for antidiabetic agents. This study followed the ICH E14 guideline to examine the effect of the oral dipeptidyl peptidase-4 (DPP-4) inhibitor linagliptin on the cardiac QT interval in healthy volunteers.Methods: This thorough QT study of linagliptin was performed in healthy subjects, using 5 mg (therapeutic dose) and 100 mg. The randomised, single-dose, placebo-controlled, double-blind, four-way crossover st...

ea0022p293 | Diabetes | ECE2010

Emblica officinalis stimulates the secretion and action of insulin and inhibits starch digestion and protein glycation

Kasabri Violet , Flatt Peter , AbdelWahab Yasser

Traditional plant treatments have been used throughout the world for the therapy of diabetes mellitus. The aim of this study was to investigate the efficacy and mode of action of Emblica officinalis Gaertn (Euphorbiaceae) used traditionally for treatment of diabetes in India. E. officinalis aqueous extract stimulated basal insulin output and potentiated glucose-stimulated insulin secretion concentration-dependently in the clonal pancreatic beta cell line, BRIN-BD...

ea0022p294 | Diabetes | ECE2010

In-patients insulin therapy protocol: establishment and valuation

Garcia Clara , Morales Cristobal , Garcia-Quiros Juan , Tome Monica , Fernandez Ignacio , Serrano Isabel , Sendon Angel

Introduction: An insulin therapy protocol (ITP) was created during 2007 in our hospital by endocrinologists, internal physicians and nurses. The aim was to establish the basal-bolus therapy as the treatment for the non-critical diabetic in-patients in order to ease the management and lead a better glycemic control of these patients. A poster was presented to all the hospital departments in several meetings during 2008. This poster showed the way to calculate the initial insuli...

ea0022p295 | Diabetes | ECE2010

Insulin treatment intensification to basal-bolus in diabetic patients

Garcia-Quiros Juan , Tome Monica , Fernandez Ignacio , Garcia Clara , Morales Cristobal , Serrano Isabel , Sendon Angel

Objectives: To valuate the insulin treatment intensification efficacy (from three premixed insulin doses to basal-bolus therapy) in type 1 and 2 diabetic patients wrongly controlled.Material and methods: The 61 patients were included. 29 (47.5%) type 1 diabetes mellitus (DM) and 32 (52.5%) type 2 DM, attended in the Day Diabetes Hospital (DDH) during 2008. 50.8% were females and 49.2% were males. Mean age was 34.2±14.4 in type1 DM and 63.6±8.6 ...

ea0022p296 | Diabetes | ECE2010

Prevalence of cardiovascular risk factors at the time of diagnosing type 2 diabetes mellitus: follow up in the endocrinologist's consultation

Tome Monica , Morales Cristobal , de Quiros Juan Manuel Garcia , Fernandez Ignacio , Garcia Clara Socorro , Serrano Isabel , Sendon Angel

Objectives: Our aim is to assess the cardiovascular risk factors that are already present at the time of diagnosing new-onset type 2 diabetes mellitus (DM).Materials and methods: This was an observational study. From each patient the following data were retrospective collected between January 2008 and September 2009: age, sex, DM in the family, smoking, HbA1c, hypertension (HTA) and dyslipidemia, microangiopathy and macroangiopathy, lipids and medical tr...

ea0022p297 | Diabetes | ECE2010

Evaluation of effect of dose and duration of treatment with metformin on serum vitamin B12 levels in type 2 diabetic patients

Harsha K P , Niveditha G , Sanjay Reddy , Kumar K M Prasanna , Shivamurthy M C

Introduction: Metformin induced impaired vitamin B12 absorption leading to fall in serum cobalamin levels has been described in literature as early as 1971. Approximately 10% of patients and in some studies 30% of patients on metformin therapy developed metformin related cobalamin deficiency. The risk of cobalamin deficiency is comparatively more among patients with vegetarian diet than in non-vegetarians.Objective: To evaluate the effect of dose and dur...

ea0022p298 | Diabetes | ECE2010

The IMPERIAL study: physicians' perceptions regarding intensification of insulin therapy

Kalra Sanjay , Kalra Bharti , Kumar Satish

The Indian Medical Practitioners Expressions Regarding Drug Interventions in All Aspects of Metabolic Syndrome (IMPERIAL) study-5 was planned to assess physicians’ perceptions regarding the relative importance and effectiveness of various methods used to upgrade or intensify insulin therapy in patients not achieving glycemic control.Seventy-four diabetologists/endocrinologists, with clinical experience of 20.25±5.0 years, attending a conference...

ea0022p299 | Diabetes | ECE2010

A case of spontaneous pneumomediastinum and diabetic ketoacidosis in patient with diabetes mellitus which was undiagnosed before

Kim Doo-Man , Kim Sunwoo , Yoo Soonjib

Introduction: Pneumomediastinum is a rare complication of diabetic ketoacidosis (DKA). The pathophysiology of DKA commonly associated with changes in pressure gradients in the pulmonary alveoli secondary to vomiting and/or Kussmaul respiration. We report a 21-year-old male with DKA who have no history of diabetes mellitus before and was found to have pneumomediastinum and subcutaneous emphysema.Case: A 21-year-old male without previous history of diabete...

ea0022p300 | Diabetes | ECE2010

Inpatient hypoglycaemia: the continuing problem and need for a proactive management protocol

Sobajo Cassandra , Rao-Balakrishna Prasanna

Hypoglycaemia is defined as capillary blood glucose (CBG) of ≤4 mmol/l irrespective of symptoms. We audited the recognition and management of inpatient hypoglycaemia with a view to identify potential areas for improvement.Methods: A total of 120 inpatients with diabetes were identified in the hospital on a single day, of whom n=32 had ≥1 episode(s) of hypoglycaemia, as evidenced by their CBG. Data relating to recognition and management...

ea0022p301 | Diabetes | ECE2010

Hypovitaminosis D and microvascular complications in diabetic patients

Garcia Elena , Martinez Guillermo , Sanchez Raquel , Hawkins Federico

Background: High prevalence of hypovitaminosis D in diabetic patients has been reported and has been associated with microvascular complications and insulin treatment in type 2 diabetic patients.Methods: Observational study in 92 inhospital patients with different types of diabetes. We analyzed the relationship between serum 25-hydroxyvitamin D (25-OHD) concentration and the clinical features associated with diabetes.Results: Of 36...

ea0022p302 | Diabetes | ECE2010

Effects of interleukin-4 on energy metabolism

Chang Yih-Hsin , Ho Kuo-Ting , Shiau Ming-Yuh

Abundant evidence has demonstrated that long-term cytokine-mediated inflammation is a risk factor for obesity and type 2 diabetes mellitus. In this study, we focused on investigating the putative involvement of Th2-derived cytokine, interleukin-4 (IL-4), in energy metabolism. Mice were i.p. injected with adenovirus containing full-length IL-4 gene, or with recombinant mouse IL-4 every other day for 8 weeks. Effects of IL-4 on energy metabolism in insulin-target cells and blood...

ea0022p303 | Diabetes | ECE2010

Metabolic control in patients with type 1 diabetes mellitus in Belarus

Rusalenko Maryia , Mokhort Tatiana

In spite of achievements in diabetology on insulinotherapy optimization, the number of patients with T1DM, who do not achieve target indicators of compensation, remains to be high.Objective: To estimate compensation and frequency of self-control carrying out among patients with T1DM in Belarus.Materials and methods: Total 965 patients with T1DM (467m, 498f), mean age (M±m) 34.33±0.51 years (1.80–72.75), age of T1D ma...

ea0022p304 | Diabetes | ECE2010

Psychological rehabilitation efficiency in adolescents with type 1 diabetes

Rusalenko Mariya , Mokhort Tatiana

Objective: To estimate psychological rehabilitation efficiency at adolescents with T1DM.Materials and methods: Total 58 adolescents with T1DM (30m, 28f), mean age (M±σ) 15.1±2.8 years (13.00–18.00), age of T1DM manifestation 5.1±3.9 years, were provided with the PedsQL4.0 questionnaire, scales of psychological well-being (SPWB). The rehabilitation program was developed: medical unit (lessons in diabetes school and doctor’s c...

ea0022p305 | Diabetes | ECE2010

Latent autoimmune diabetes of adults and its clinical, immunological and genetics markers

Tikhonova Tatyana , Karachentsev Yurii , Shtandel Svetlana , Levchenko Tamara

The aim of the study was to determine the clinical, immunological and genetics markers of latent autoimmune diabetes of adults (LADA) in early terms of disease development.Materials and methods: Seventy-eight LADA patients (47 women, 31 men, age of manifestation 45.52±1.83 years, average age 49.37±1.17 years, insulindependence age 49.33±0.34 years with anti islet, GADA antibodies and low C-peptide levels) were enrolled. The GADA, IA2A and ...

ea0022p306 | Diabetes | ECE2010

Exercise-related levels of acetylcarnitine in skeletal muscle of individuals with type 1 diabetes in Eu- and hyperglycaemia assessed by 1H-MR spectroscopy

Jenni Stefan , Boss Andreas , Kreis Roland , Ith Michael , Christ Emanuel , Boesch Chris , Stettler Christoph

Background: Acetylcarnitine (AC) is involved in intramyocellular fuel metabolism during exercise. So far, little is known on exercise-associated myocellular AC levels in patients with type 1 diabetes. In particular, it is unknown whether AC levels differ according to glucose values in diabetic patients. 1H magnetic resonance spectroscopy (MRS) of the skeletal muscle allows for the non-invasive assessment of acetylcarnitine (AC) accumulation. The present study assess...

ea0022p307 | Diabetes | ECE2010

Association between dyslipidaemia and micro and macrovascular complications in type 2 diabetic patients

del Canizo-Gomez Francisco Javier , Rodriguez Manuela Belen Silveira , Gonzalez-Losada Tomas , Moreno-Ruiz Inmaculada , de Gorospe Perez-Jauregui Carlos , Segura-Galindo Amparo

Various risk factors act synergistically for the development of micro and macrovascular complications in type 2 diabetes mellitus (T2DM) patients. The contributions of risk factors have yet to be clearly identified and quantified, but diabetic dyslipidaemia has specific importance.Objective: To investigate the association of dyslipidaemia with micro and macrovascular complications in a T2DM population.Materials and methods: Cross s...

ea0022p308 | Diabetes | ECE2010

Pattern of type 2 diabetes mellitus in Surabaya, Indonesia

Handayani Olivia , Tandra Hans

Objective: To observe the presentation, anthropometry, and complication of type 2 diabetes (T2D) in diabetes clinic in Surabaya, Indonesia.Methodology: It is a retrospective review of diabetic cases over 12 months. Data extracted from medical records, DM patients demographic and clinical laboratory parameters.Results: We investigated 302 T2D patients, 132 males and 170 female, with mean age of 55.9±21.1 years. Mean duration of...

ea0022p309 | Diabetes | ECE2010

Metabolic syndrome in patients with type 2 diabetes mellitus in Surabaya, Indonesia

Handayani Olivia , Tandra Hans

Objective: To determine the frequency of metabolic syndrome (MS) in adult patients with type 2 diabetics (T2D) in Surabaya, Indonesia based on NCEP-ATP III.Methods: This is a retrospective study. All consecutive adults with T2D were evaluated for the presence or absence of MS. According NCEP-ATP III, metabolic syndrome (MS-ATP III) was defined as two of four of the following: central obesity (waist circumference >102 cm in males and >88 cm in fem...

ea0022p310 | Diabetes | ECE2010

Profile of patients with type 2 diabetes mellitus in Surabaya, Indonesia

Handayani Olivia , Tandra Hans

Objective: To document the anthropometrics indices and other characteristics of patients with type 2 diabetes who attending the clinic of diabetes.Methodology: The patient’s data, anthropometric indices, co-morbidities and pattern of therapies were documented in a cross-sectional study of 500 patients attending Diabetes Clinic of Surabaya, Indonesia over a 3-month period. Quantitative data were analyzed using the statistic Student’s t-te...

ea0022p311 | Diabetes | ECE2010

Risk estimation of hypoglycemic episodes among patients with type 1 diabetes mellitus

Makhlina Elena , Mokhort Tatiana

Study objective: To determine risk factors of hypoglycemic episodes among patients with type 1 diabetes mellitus (T1D) using the continuous glucose monitoring system (CGMS).Materials and methods: Total 120 female patients with T1D were examined. The 1st group was with concealed hypoglycemic episodes (n=79) that has made 66% and the 2nd group was with frank hypoglycemic episodes (n=41) that has made 31% of the total number of the examined. T...

ea0022p312 | Diabetes | ECE2010

The effects of short-term fasting on insulin sensitivity and inflammation in healthy subjects

Dumanovic Mirjana Sumarac , Micic Dragan , Pejkovic Danica Stamenkovic , Jeremic Danka , Cvijovic Goran , Stefanovic Darko , Trajkovic Vladimir

It is well known that weight losses in the range of 2.5–5% are able to improve factors of the metabolic syndrome such as insulin sensitivity. Several studies have been published concerning weight loss and subsequent changes in inflammatory markers and indicate that higher weight losses are necessary to affect the inflammatory markers than are needed to affect insulin sensitivity. Some data showed impairment of insulin sensitivity in diabetic patients during starvation. Th...

ea0022p313 | Diabetes | ECE2010

Fifty-two-week treatment with diet and exercise plus transdermal testosterone improves biomarkers of non-alcoholic fatty liver disease and cardiovascular risk in hypogonadal men with the metabolic syndrome

Saad Farid , Heufelder Armin , Bunck Mathijs , Gooren Louis

Objectives: Men with the metabolic syndrome (MetS) and type 2 diabetes (T2D) often have low testosterone levels. Elevating low testosterone levels may improve features of the MetS and glycemic control. In this analysis, we assessed effects of normalization of circulating testosterone on biomarkers of non-alcoholic fatty liver disease (NAFLD), and cardiovascular risk.Design and methods: In a single-blind, 52-week clinical trial, 32 hypogonadal men with th...

ea0022p314 | Diabetes | ECE2010

Erythropoietin and vascular endothelial growth factor as risk markers for severe hypoglycaemia in type 1 diabetes

Kristensen Peter Lommer , Pedersen-Bjergaard Ulrik , Olsen Niels Vidiendal , Schalkwijk Caper , Thorsteinsson Birger

Aims/hypothesis: Circulating erythropoietin (EPO) and vascular endothelial growth factor (VEGF) increase during hypoglycaemia and may represent a protective hormonal counter-regulatory response. We tested the hypothesis that low levels of EPO and VEGF are associated with a higher frequency of severe hypoglycaemia in a cohort of patients with type 1 diabetes.Methods: Two hundred and nineteen patients with type 1 diabetes (41% females, age 46±13 years...

ea0022p315 | Diabetes | ECE2010

Vitamin D deficiency and isolated fasting hyperglycemia in pregnancy

Vasileiou Vasiliki , Athanasiadou Anastasia , Giakoumi Athanasia , Papageorgiou George , Zapanti Evangelia , Alevizaki Maria , Anastasiou Eleni

Introduction: The relation between vitamin D deficiency and gestational diabetes mellitus (GDM) has been rarely addressed in the literature with conflicting results. The aim is to determine the association between maternal serum 25(OH)D and glucose metabolism in Caucasian pregnant women.Patients and methods: In a prospective study 157 pregnant women aged 31.2±5.8 years underwent a 100 g OGTT in the third trimester of pregnancy, during which serum 25...

ea0022p316 | Diabetes | ECE2010

Postpartum evaluation of patients with gestational diabetes and newborns characteristics: differences between two periods

Gil Beatriz Mantinan , Luna Cano Reyes , Sobrino Paula Sanchez , Carballeira Regina Palmeiro , Cruz Ines Seoane , Fernandez Concepcion Paramo , Garcia Mayor Ricardo Victor

Introduction: Blood glucose control during pregnancy is essential in patients with gestational diabetes (GD) to prevent fetal complications. Despite advances in its control, neonatal morbidity and mortality still appears in a significant percentage of cases. The presence of GD also implies consequences for the mother, as the GD appearance in a new pregnancy and/or the future development of glucose metabolism alterations.Methods: We conducted a retrospect...

ea0022p317 | Diabetes | ECE2010

Thyroid diseases in patients with type 1 diabetes mellitus

Dirikoc Ahmet , Kuru Meryem , Sen Didem Ozdemir , Aydin Cevdet , Ersoy Reyhan , Cakir Bekir

Introduction: Coexistence of type 1 diabetes mellitus (DM) and autoimmune thyroid diseases was shown in previous studies. Thyroid dysfunctions and thyroid autoantibody positiveness were reported in 23–25 and 27–44% of patients with type 1 DM, respectively. In recent years, thyroid ultrasonography (US) is widely used to diagnose autoimmune thyroid diseases with regard to its ability to define thyroid morphology and echogenity. In this study, we aimed to evaluate thyro...

ea0022p318 | Diabetes | ECE2010

Proinflammatory cytokines and serum lipids in patients with diabetes mellitus 2

Tzioras Constantinos , Brionidou Adromaxi , Klonaris Nikos , Bantila Alexandros

Introduction: Recently, several cytokines attracted considerable attention as potential effectors of insulin resistance and endothelium dysfunction, both associated with type 2 diabetes mellitus (T2DM) pathophysiology.Serum lipids disturbances also observed in inflammation and immunologic disorders have been found to largely depend on proinflammatory cytokines release.Aim: To establish the presence of cytokines levels disturbances ...

ea0022p319 | Diabetes | ECE2010

Non-invasive cytological method of diagnostics and monitoring of type 2 diabetes mellitus development

Yui Rudolf , Mansharipova Alma , Mulkibayeva Sholpan , Idrisov Alisher

Material for cytological analysis was oral swab of investing type (lip, cheek) in 22 healthy people and 33 sick ones with type 2 diabetes mellitus at sub compensational stage. The group under study was homogenous in age (45–65), sex (men) and biorhythmic type. Experiments were performed on human subjects and local Ethical Committee approval was obtained. Oral swabs were fixed in spirit–acetone and were May–Grunvald’s and Romanovsky–Gimsa’s dyed. T...

ea0022p320 | Diabetes | ECE2010

Calcaneal spur incidence is increased in patients with type 2 diabetes mellitus

Aydogdu Aydogan , Akbulut Halil , Uckaya Gokhan , Taslipinar Abdullah , Sonmez Y Alper , Aydogan Umit , Kutlu Mustafa

Obesity is a risk factor for calcaneal spur (CS) formation which is supposed to originate from chronic plantar fasciitis. But histological findings support the thesis that ‘plantar fasciitis’ is a degenerative fasciosis without inflammation. Diabetes mellitus may contribute to the risk of CS by decreased ability of tissue repair and increased reactive ossification. Thus, we aimed to determine CS incidence in asymptomatic obese subjects with and without type 2 diabete...

ea0022p321 | Diabetes | ECE2010

Analysis of the clinical course of patients with type 2 diabetes mellitus from onset of insulin treatment (EDIN study): baseline characteristics of patients

Rodriguez Angel , Caveda Elena , Reviriego Jesus

Introduction: The EDIN study evaluated the clinical course of patients with type 2 diabetes mellitus (T2DM) from the start of the insulin treatment to a minimum of 5 years, within routine clinical practice in Spain.Objective: To describe the characteristics of patients at onset of insulin treatment.Methods: Observational study that consecutively included patients with T2DM, ≥30 years old and treated with insulin for at least ...

ea0022p322 | Diabetes | ECE2010

Badly controlled hyperglycaemia provokes arrhythmia and may increase cardiovascular risk in diabetic patients

Smirnov Ivan , Ilchenko Irina , Zhuravlyova Larysa

Cardiovascular risk (CR) in patients with diabetes mellitus (DM) is high. Arrhythmias (Ar) could increase CR as well. We aimed to define is there any association between hyperglycemia and incidence of Ar. We studied 137 (75 F/62 M) type 2 diabetic patients with coronary artery disease (CAD). The age was 54.0±5.5 years (M±S.D). The CAD and DM duration <10 years was in 64 (47%) and in 47 (34%), 15 years and above – in 21 (15%) and in 34 (25%) re...

ea0022p323 | Diabetes | ECE2010

Etiologies of patients admitted to emergency department with hypoglycemia

Eren Hakan , Caliskan Haci Mehmet , Kilicli Fatih , Korkmaz Ilhan , Acibucu Fettah , Dokmetas Sebila

Introduction: Hypoglycemia is the most common endocrinological problem in emergency departments.Objective: To explore the causes of hypoglycemia in patients who were admitted to the emergency department with symptoms resembling hypoglycemia.Design: In our study, 225 hypoglycemia cases were evaluated from January 2000 to May 2008.Results: Of 225 hypoglycemia cases, 158 (70.2%) receiving treatment for diabetes ...

ea0022p324 | Diabetes | ECE2010

Dose--response characteristics for effects of insulin in patients with type 2 diabetes and severe insulin resistance: evidence for the utility of high doses of insulin

Kampmann Ulla , Hoyem Pernille , Mengel Anette , Orskov Lotte , Moller Niels

Background: To avoid the serious complications of diabetes glucose control must be tackled aggressively in type 2 diabetes. However some patients with type 2 diabetes are severely insulin resistant and although they are treated with large doses of insulin their blood glucose is still not optimally controlled.Aim: This project aims to determine the dose–response to insulin in patients with type 2 diabetes and severe insulin resistance.<p class="a...

ea0022p325 | Diabetes | ECE2010

Interrelation of changes of interval QT and indexes of metabolic control in children with diabetes mellitus type 1

Meraai Galina , Solntseva Angelika

The basic manifestations of dystrophic changes in heart of children suffering from diabetes mellitus type 1 (DM1) are disturbances of repolarization and depolarization processes, including QT and QTc intervals prolongation. Research objective: to evaluate indices of QT and QTc in children with DM1, to reveal interrelation of the given changes with the duration of disease, age, sex, levels of cholesterol (CH), of glycemie, BMI, blood pressure, pulse rate....

ea0022p326 | Diabetes | ECE2010

Pancreatic insulin secretion in women with polycystic ovary syndrome

Vrbikova Jana , Bilek Radovan , Vankova Marketa , Vondra Karel , Bendlova Bela

Aim: To study the insulin sensitivity and secretion in women with polycystic ovary syndrome (PCOS).Subjects and methods: Thirty-eight women with PCOS (age 26.1±4.1 years, BMI 20.9±2.0 kg/m2) and 81 control healthy women (age 25.8±5.2 years, BMI 21.1±2.3 kg/m2) underwent oral glucose tolerance test (OGTT). Insulin resistance was evaluated as oral glucose index (OGIS); Matsuda and HOMA-IR, insulin secretion was cal...

ea0022p327 | Diabetes | ECE2010

Chitotriosidase activity predicts endothelial dysfunction in type 2 diabetes mellitus

Haymana Cem , Sonmez Alper , Tapan Serkan , Safer Umut , Celebi Gurkan , Ozturk Ozlem , Genc Halil , Dogru Teoman , Tasci Ilker , Erdem Gokhan , Taslipinar Abdullah , Aydogdu Aydogan , Yilmaz Mahmut I , Kurt Ismail , Kutlu Mustafa

The enzyme chitotriosidase (ChT) is secreted by activated macrophages and play active role in human immune response. ChT activity is increased in atherosclerosis in association to the extent of the disease. We investigated the relevance of ChT to endothelial functions and insulin resistance in patients with T2DM.Forty newly diagnosed and untreated patients with T2DM (male 17; age: 47.0±6.2 years) and 50 healthy volunteers (male 21; age: 50.2±8....

ea0022p328 | Diabetes | ECE2010

Follow up of type 1 diabetic patients treated with insulin continuous s.c. infusion

Andia Victor M , Fernandez Elisa , Jara Antonino , Garcia Rogelio , Sambo Marcel , Lezcano Diego A

Insulin continuous s.c. infusion (ICSI) is an alternative therapy in selected diabetic patients when a good metabolic control is not achieved with multiple insulin doses (MID). We report the follow-up of patients with ICSI at least during 1 year in our centre.Patients and methods: Twenty-five patients (16 females, 9 males), with median age 38.4±12.5 years and diabetes evolution mean time 16.8±7.8 years, were changed from MID to ICSI due to defi...

ea0022p329 | Diabetes | ECE2010

Effects of ghrelin treatment to promote proliferation of pancreatic islet in BB rats

Baena Maria Gloria , Lomas-Romero Isabel Maria , Perez-Arana Gonzalo , Blandino-Rosano Manuel , Segundo Carmen , Aguilar-Diosdado Manuel , Lechuga-Sancho Alfonso M

Aims: BB rats develop diabetes as young adults by an autoimmune mechanism. Ghrelin is a peptidic hormone synthesized mainly in the oxintic cells of the gastric mucosae in adults. During intrauterine life however, this peptide is synthesized mainly at the pancreas. Ghrelin exerts proliferative and survival promoting effects in different cell types. We investigated the effect of ghrelin treatment in a diabetes autoimmune animal model to analyze death rate and cellular proliferat...

ea0022p330 | Diabetes | ECE2010

Lipid profile in patients with ST-segment elevation myocardial infarction with or without diabetes

Mladenovic Violeta , Djukic Aleksandar , Petrovic Jelena , Zdravkovic Vladimir

Hyperlipidemia is common among patients with ST-elevation myocardial infarction (STEMI) and is associated with high risk of mortality and morbidity. Hypertriglyceridemia and low HDL cholstereol are independent risk factor for cardiovascular disease.Aim: The aim of this study is to analyse lipid profile in patients with STEMI with and without diabetes.Material and methods: This study included all patients registered in Coronary Unit...

ea0022p331 | Diabetes | ECE2010

Transient insulin resistance during the laparoscopic versus open cholecystectomy

Micic Dusan , Polovina Snezana , Micic Jelena , Loncar Zlatibor , Micic Dragan , Djukic Vladimir

Aim: The aim of our study was to investigate is surgical stress induce alterations in insulin sensitivity in the course of cholecystectomy performed in two differnt procedures: as a open surgery and as laparascopic operation.Material and methods: The homeostasis assessment model (HOMA) was used to determine insulin sensitivity before and in the first, third and seventh day after the operation. Patients were divided int two groups: group A (open cholecyst...

ea0022p332 | Diabetes | ECE2010

The mean HbA1c values in T2DM according a therapy regiments

Polovina Snezana , Nemet Klara Tucic , Micic Dusan , Micic Jelena , Micic Dragan

Aim: To calculate mean HbA1c values in patients with type 2 diabetes mellitus in different therapy regiments.Material and methods: Ninety-nine diabetic persons was divided in three groups: group I (n=33) on oral hypoglucemic agents, group II (n=33) on one or two doses of insulin and group III (n=33) on intensive insulin therapy. We have calculated the mean values of HbA1c on the begining and 6 months after start of therapy regiment.<...

ea0022p333 | Diabetes | ECE2010

Therapy with exenatide: our experience

Fernandez Ignacio , Morales Cristobal , De Quiros Juan Manuel Garcia , Tome Monica , Garcia Clara , Serrano Isabel , De La Cuesta Carmen , Sendon Angel

Objectives: Verify the efficacy of the GLP-1 analogue exenatide in patients with type 2 DM assisted in our hospital area.Material and methods: This study included patients who initiated this therapy in 2008 and 2009. Through 3 months were analyzed the outcomes of weight, HbA1c and the development of adverse effects.Results: The process included 32 patients, 21 men and 11 women, with ages between 26 and 73 years old (mean age 48.78 ...

ea0022p334 | Diabetes | ECE2010

Prevalence of metabolic syndrome and diabetes mellitus type 2 under the conditions of the primary component of public health

Shagazatova Barno , Stareva Viktoria , Mukhiddinova Gavhar

Background and aims: The metabolic syndrome (MS) – pathology, that is in the center of the attention of endocrinologists, cardiologists and therapeutics, is frequently diagnosed with the development of vascular complications, or of diabetes mellitus type 2 (DMT2).The aim of the present study is to investigate the occurrence of MS, according to IDF (2005) and DMT2 according to criteria WHO (1999) under the conditions of the primary component of publi...

ea0022p335 | Diabetes | ECE2010

Adiponectin time dependence secretion under β3 agonist, PPAR agonist treatments

Hodis Jiri , Farghali Hassan

Background and aims: Adiponectin produced by visceral adipocytes is believed to be marker of insulin sensitivity. Glitazones (PPARγ agonists) induce adipocetin transcription and secretion. β3 agonists are potencial antiobesity drugs with lipolytic activity. We try to shed light on the relationship between PPARγ, β3 signaling, and adiponectin secretion.Methods: Epididymal rat adipocytes were isolated and cultivated together with trogli...

ea0022p336 | Diabetes | ECE2010

Therapeutic objectives in the care of patients with diabetes mellitus type 2 in clinical practice at an endocrine department

Jimenez Carmen Tenorio , Ibarra Pablo Jose Lopez , Munoz Manuel Romero , del Valle Maria Jimenez

According to the American Diabetes Association (ADA), achieving specific treatment goals in type 2 diabetes can substantially reduce microvascular complications, including retinopathy, nephropathy and neuropathy. Intensive management has also been shown to have a beneficial effect on cardiovascular disease. The objective of the study is to evaluate the degree of achievement of appropriate target levels for A1C, as an index of chronic glycaemia, LDL-c and blood pressure, in a l...

ea0022p337 | Diabetes | ECE2010

The effect of short-term metformin therapy on C-reactive protein and insulin sensitivity in newly diagnosed patients with type 2 diabetes mellitus

Micic Dragan , Dumanovic Mirjana Sumarac , Jeremic Danka , Cvijovic Goran , Polovina Snezana

C-reactive protein (CRP) is closely associated with obesity and cardiovascular disease in both diabetic and nondiabetic subjects. The aim of our study was to investigate the effects of commonly prescribed antidiabetic agent metformin on CRP and insulin sensitivity in type 2 diabetes patients after 12 weeks of therapy. Twenty-three newly diagnosed type 2 diabetic patients (mean age: 49.74±2.03 years; mean BMI: 31.40±1.27 kg/m2) have been given metformin 2 g...

ea0022p338 | Diabetes | ECE2010

Vitamin D and glycaemic control

Kostoglou-Athanassiou Ifigenia , Badila Razvan Alexandros , Athanassiou Panagiotis , Michou Aikaterini , Chronaiou Aikaterini , Stefanopoulos Dimitris , Terzi Thomais , Karfi Areti

Introduction: Research results from different countries show that vitamin D deficiency is a modern reality, affecting even sunny countries. Vitamin D deficiency has been related to predisposition to the development of diabetes mellitus type 2 and it may affect the secretion and action of insulin.Aim: The aim was to study the effect of vitamin D on glycaemic control in patients with diabetes mellitus type 2.Materials and methods: Th...

ea0022p339 | Diabetes | ECE2010

Maternal obesity and gestational diabetes mellitus

Kourtis Anargyros , Giomisi Athina , Makedou Kali , Mouzaki Maria , Kalogiannidis Ioannis , Traianos Alexandros , Prapas Nikolaos , Agorastos Theodoros

Objective: Numerous studies have reported an increased risk of gestational diabetes mellitus (GDM) among women who are overweight or obese compared with lean or normal-weight women. Obesity contributes to multiple health problems during pregnancy and predisposes a woman to develop GDM. Obesity is defined as a body mass index (BMI) of >30. Overweight is defined as a BMI of 25.0–29.9 and normal weight is defined as a BMI 20–24.9. The aim of the present study was to...

ea0022p340 | Diabetes | ECE2010

Performances of the fully automated Liaison® system for determination of insulin and C-peptide concentrations

Gruson Damien , Preumont Vanessa , Vandeleene Bernard

Introduction: Circulating insulin levels are widely used to provide diagnostic information for diabetes mellitus and hypoglycaemic syndromes. C-peptide concentration provides an accurate assessment of residual B-cell function and reliable information about insulin secretion in patients with diabetes. The aim of this study was to evaluate the performances and reliability of the Liaison® fully automated system for measurement of insulin and C-peptide concentratio...

ea0022p341 | Diabetes | ECE2010

Role of regulatory T-cells in the development of the partial remission period in patients with type 1 diabetes

Nikonova Tatiana , Gorelysheva Vera , Prokofiev Sergey , Pekareva Elena , Dedov Ivan

Background and aims: Remission period in type 1 diabetes (T1D), also known as the honeymoon period has been acknowledged as an advantageous time for immune intervention. However, not all subjects will go through this remission phase. It was suspected that defects in regulatory T-cells number and activity are causally related to the development of T1D.The aim of the study was to find specific T-cell assay that reflects remission period.<p class="abste...

ea0022p342 | Diabetes | ECE2010

Lipid profile in women with gestational diabetes mellitus

Kourtis Anargyros , Makedou Kali , Giomisi Athina , Mouzaki Maria , Masoura Sofia , Goutzioulis Antonios , Prapas Nikolaos , Agorastos Theodoros

Background-aim: Increased serum cholesterol and triglycerides levels may have a role in the development of gestational diabetes mellitus (GDM) and its complications. This study was designed in order to provide a description of lipids profile by gestational age during pregnancy.Subjects and methods: We studied 211 women from the outpatient clinic of metabolic disorders in pregnancy. These women were ordered to perform the oral glucose tolerance test (OGTT...

ea0022p343 | Diabetes | ECE2010

Increased insulin resistance is associated with increased urinary excretion of chromium in non-diabetic, normotensive Saudi adults

Bahijri Suhad , Alissa Eman

Background: The role of trivalent chromium (Cr3+) in improving glucose tolerance, and decreasing insulin resistance is well documented in animals and humans, however supplementation studies gave inconsistent results. Increased urinary excretion of chromium has been reported in type 2 diabetes mellitus (DM). It was not known whether this renal wasting had preceded DM, or was caused by it.Aim: Our aim was to investigate the relationship between ...

ea0022p344 | Diabetes | ECE2010

Systemic effect of insulin on local cytokine concentrations in adipose tissue and muscle in healthy humans

Mader Julia K , Ikeoka Dimas , Weinhandl Heinz , Pachler Christoph , Bock Gerlies , Plank Johannes , Ellmerer Martin , Pieber Thomas R

Introduction: Cytokines are produced in different tissues under physiological and pathophysiological conditions. The present study was performed to compare local concentrations of cytokines in subcutaneous adipose tissue and muscle using the open flow microperfusion (OFM) technique, as well as to investigate systemic effects of insulin on interstitial concentrations of these mediators.Methods: Ten healthy volunteers (age: 27±4 years, BMI: 22.6±...

ea0022p345 | Diabetes | ECE2010

Decreased serum brain-derived neurotrophic factor concentration in young nonobese subjects with low insulin sensitivity

Karczewska-Kupczewska Monika , Adamska Agnieszka , Nikolajuk Agnieszka , Otziomek Elzbieta , Gorska Maria , Straczkowski Marek , Kowalska Irina

Insulin resistance and type 2 diabetes are associated with an increased risk of neurodegenerative diseases. Brain-derived neurotrophic factor (BDNF) regulates neuronal differentiation and synaptic plasticity and its decreased levels are supposed to play a role in the pathogenesis of Alzheimer disease and other disorders. Decreased circulating BDNF levels in obesity and type 2 diabetes were reported, however, it is unclear, whether BDNF might be associated with insulin resistan...

ea0022p346 | Diabetes | ECE2010

Microvascular reactivity and oxidative stress after standard breakfast in patients with recently diagnosed type 2 diabetes

Horova Eva , Prazny Martin , Skrha Jan

Aim of the study: The aim of the study was to compare skin microvascular reactivity (MVR) with oxidative stress and metabolic parameters at fasting status and postprandially in patients with recently diagnosed type 2 diabetes.Methods: Twenty patients with type 2 diabetes (mean age 58±6 years, HbA1C 4.8±0.5%, diabetes duration 2.3±1.3 years, metformin treatment only) were included in the study. Blood samples were taken before and after 60, ...

ea0022p347 | Diabetes | ECE2010

Quantitative and qualitative changes in T regulatory lymphocytes (Tregs) in newly-diagnosed patients with type 1 diabetes

Paschou Stavroula , Vartholomatos George , Kolaitis Nikolaos , Papadopoulos George , Tsatsoulis Agathocles

Introduction: T regulatory lymphocytes (Tregs) are defined as CD4+CD25high T lymphocytes and are thought to regulate immune tolerance. The Foxp3 protein level in Tregs is proportional to CD25 protein surface expression and regulatory function, but inversely proportional to IL-7R (CD127) level, the receptor for the growth and survival of T cells. We investigated possible differences in Tregs between newly diagnosed type 1 diabetes (DM1) patients and contro...

ea0022p348 | Diabetes | ECE2010

Vanishing testes syndrome associated with diabetes mellitus

Djrolo Francois

A 30-year-old man complaining of polyuria, polydipsia and asthenia was seen in consultation with a plasma glucose level of 3.97 g/l. He was discovered diabetic 1 year ago and treated with glibenclamide, which was stopped by the patient after 5 months because of lack of money. There was no family history of diabetes. Physical examination showed an eunuchoid status man with micropenis, hypoplastic scrotum with absence of testes confirmed by ultrasonographic exploration. Three mo...

ea0022p349 | Diabetes | ECE2010

The high prevalence of abnormal cardiac function in diabetic patients of a developing community

Bazrafshan Hamid , Ramezani Mohammad Ali , Shirafkan Ahmadali , Ahangar Hasan , Gholamrezanezhad Ali

Introduction: Cardiac complications are one of the most important long-term adverse effects of diabetes mellitus (DM), which is usually under diagnosed, as it is frequently asymptomatic. As it seems that the control of DM in developing nations is not optimal, it can be predicted that the prevalence of cardiac complications of DM is subsequently high. We assessed the prevalence of such complications in a developing community in the north of Iran.Method: T...

ea0022p350 | Diabetes | ECE2010

The GG genotype of the G-11391A ADIPOQ polymorphism might be a risk factor for development of type 2 diabetes mellitus in women

Polosak Jacek , Roszkowska-Gancarz Malgorzata , Kurylowicz Alina , Turowska Olga , Franek Edward , Puzianowska-Kuznicka Monika

Background: Adiponectin is a protein hormone secreted by adipocytes. Its expression is higher in lean than in obese individuals and in women than in men. It inhibits expression of gluconeogenic enzymes and the rate of glucose production. It also stimulates glucose utilization and fatty acids oxidation. Adiponectin is encoded by a highly polymorphic ADIPOQ gene. The −11377C>G, −11391G>A, and −11426A>G ADIPOQ polymorphisms modulate cir...

ea0022p351 | Diabetes | ECE2010

Increased serum nesfatin-1 levels in patients with impaired glucose tolerance

Gulcelik Nese Ersoz , Akin Safak Cavus , Aksoy Duygu Yazgan , Karakaya Jale , Usman Aydan

Objective: Nesfatin was found to be co-localized with insulin in pancreatic β cells and its levels were decreased in type 2 diabetic patients. In order to investigate the role of nesfatin in carbohydrate metabolism, we measured baseline nesfatin-1 levels and its response to glucose load in patients with impaired glucose tolerance (IGT) and in healthy subjects.Subjects and methods: Fourteen patients with IGT and, 13 age and body mass index matched co...

ea0022p352 | Diabetes | ECE2010

TCF7L2 gene variants and polycystic ovary syndrome

Vankova Marketa , Vcelak Josef , Lukasova Petra , Bradnova Olga , Prazakova Silva , Dvorakova Katerina , Vrbikova Jana , Bendlova Bela

Polycystic ovary syndrome (PCOS) is considered as a risk factor for diabetes type 2 (DM2), it is often associated with obesity, β-cell dysfunction or insulin resistance. Genetic factors underlying the association of PCOS with DM2 is unknown. Recent human genetic studies have revealed that transcription factor 7-like (TCF7L2) polymorphisms are associated with DM2 through modulation of β-cell function.The aim of this study was to test if <...

ea0022p353 | Diabetes | ECE2010

Insulin sensitivity and acute insulin response in pateints with primary hyperparathyroidism before and after surgical treatment

Cvijovic Goran , Micic Dragan , Kendereski Aleksandra , Zoric Svetlana , Sumarac-Dumanovic Mirjana , Stamenkovic-Pejkovic Danica , Jeremic Danka

It was reported that patients with primary hyperparathyroidism (PHPT) are insulin resistant, and that surgical treatment may improve insulin sensitivity in this group of patients. The aim of our study was to evaluate the effect of surgical treatment on insulin sensitivity and acute insulin response (AIR) in patients with PHPT.Methods: In patients with PHPT (n=37; age: 57.72±8.29 years, BMI 26.22±4.37 kg/m2) euglycemic–hyp...

ea0022p354 | Diabetes | ECE2010

Renal complications of aldosteronism and diabetes: role of oxidative stress and protective effects of polyphenol extract

Elowe-Gruau Eglantine , Dal-Ros Stephanie , Auberval Nathalie , Seyfritz Elodie , Bietiger William , Pinget Michel , Jeandidier Nathalie , Sigrist Severine

Hyperaldosteronism and diabetes are two providers of kidney disease, showing molecular and cellular mechanisms in common, such as oxidative stress, causing renal tubular damages. The objective of this study is to evaluate in vitro the protective effect of a natural antioxidant (red wine polyphenols and green tea extract) in the onset of renal dysfunction. Hyperglycemia and hyperaldosteronism are induced respectively by increasing doses of glucose and aldosterone in prim...

ea0022p355 | Diabetes | ECE2010

Prediction of incident diabetes mellitus by baseline insulin-like growth factor 1 levels

Schneider Harald , Wallaschofski Henri , Stalla Gunter-Karl , Wittchen Hans Ulrich

Objective: Insulin-like growth factor 1 (IGF1) is associated with metabolic parameters and involved in glucose metabolism. Low IGF1 has been implicated in the etiology of glucose intolerance. However, epidemiological data are scarce and the role of IGF1 in prospective studies is not clear to date.Research design and methods: We measured IGF1 levels in 7665 subjects free of diabetes from two German prospective cohort studies, the DETECT study and SHIP and...

ea0022p356 | Diabetes | ECE2010

Fasting and postprandial effects of rosiglitazone on low- and high-density lipoproteins size and subclasses in type-2 diabetes

Rizzo Manfredi , Berneis Kaspar , Vekic Jelena , Rini Giovam Battista , Koulouris Spyridon , Sakellariou Dimitrios , Pastromas Socrates , Manolis Antonis

Background: There is evidence that rosiglitazone may increase cardiovascular risk and, on this basis, this agent has been recently advised for treatment of type-2 diabetes. Yet, its effects on fasting and postprandial atherogenic dyslipidemia are still not fully elucidated.Methods: In an open-label study rosiglitazone (4 mg/day for 12 weeks) was added on top of a maximum of two oral antidiabetic drugs to 18 patients with adequately controlled type-2 diab...

ea0022p357 | Diabetes | ECE2010

Levels of adrenomedullin during low dose Synacthen test in diabetics type 1

Simunkova Katerina , Bilek Radovan , Hampl Richard , Zamrazil Vaclav , Janickova-Zdarska Denisa , Hill Martin , Vondra Karel

The aim of the study was to obtain the basic data about adrenomedullin after 1 μg ACTH stimulation.Adrenomedullin has direct effect on adrenal cortex secretion, therefore we studied the changes of its concentrations during ACTH stimulation in diabetic patients suspected of hypocorticalism.Thirty-two diabetics type 1 (DM1) were investigated; 16 with and 16 without autoimmune thyroditis (AIT); age 44±10 years (mean±<sm...

ea0022p358 | Diabetes | ECE2010

Antioxidative effect of green tee and red wine polyphenol extracts on RINm5F cells after oxidative stress induction

Auberval Nathalie , Ros Stephanie , Schini-Kerth Valerie , Pinget Michel , Sigrist Severine

Diabetes is associated with an oxidative stress (OS). Free radicals are responsible of β-cell destruction in type 1 diabetes and their dysfunction in type 2. The purpose of this work is to evaluate preventive effect of antioxidative natural compounds on several models of OS induced on β-cells.The models of oxidative stress were performed with 40 μmol/l H2O2 for 30 min, with a mixture of 0.25 mmol/l hypoxanthine and 10...

ea0022p359 | Diabetes | ECE2010

Waiting time for emergency renal replacement therapy in patients with end stage renal failure secondary to diabetes mellitus

Rodriguez Florisa , Triana Javier , Tamez Hector Eloy , Robles Sofia Daniela

Introduction: The incidence of end stage renal failure (ESRF) secondary to diabetes mellitus (DM) is increasing worldwide. Renal replacement therapy, which if started early improves prognosis, is a common treatment for ESRF.Objective: To determine the waiting time and factors that influence the provision of emergency renal replacement therapy in patients with ESRF secondary to DM admitted to the emergency room of a tertiary care hospital.<p class="ab...

ea0022p360 | Diabetes | ECE2010

Obestatin and ghrelin inhibit apoptosis of human pancreatic islet endothelial cells in high glucose culture

Favaro Enrica , Zanone Maria Maddalena , Miceli Ilaria , Settanni Fabio , Camussi Giovanni , Perin Paolo Cavallo , Ghigo Ezio , Granata Riccarda

Pancreatic islet microendothelium exhibits unique structural and functional features, in an interdependent physical and functional relationship with the neighbouring β-cells. Glucose toxicity is not solely restricted to β-cells, but affects also survival, proliferation of human pancreatic islet endothelial cells, thus possibly contributing to β-cell function impairment and β-cell loss. It has been previously demonstrated that the ghrelin gene derived peptid...

ea0022p361 | Diabetes | ECE2010

Hepatic mitochondrial direct effects of thiazolidinediones: pioglitazone and rosiglitazone

Sanz Maria Nieves , Sanchez-Martin Carlos , Detaille Dominique , Bouderba Saida , El-Mir Mohammed Yehia

Introduction: Thiazolidinediones (TZDs) are synthetic insulin-sensitizing drugs used in the treatment of type 2 diabetes. They work as agonists of peroxisome proliferator-activated receptor γ (PPARg). TZDs action via PPARg does not exclude independent PPARg-activation effects. Our research group has recently reported that TZDs also execute direct effects implicated in glucose homeostasis. However, its mitochondrial action PPARg-independent has not been fully studied yet.<...

ea0022p362 | Diabetes | ECE2010

Glycemic control and treatments in adults with type 1 diabetes mellitus

Baser Husniye , Aydin Cevdet , Dirikoc Ahmet , Arpaci Dilek , Ersoy Reyhan , Cakir Bekir

Introduction: Survival is increased in childhood-onset type 1 diabetes mellitus (DM) and approximately half of patients are diagnosed after the age of 15. Thus, a considerable number of patients with type 1 DM are adults. In this study, we aimed to investigate glycemic control and modalities of treatments in adult type 1 DM patients.Material and methods: Data of type 1 DM patients hospitalized between January 2004 and December 2009 were evaluated retrosp...

ea0022p363 | Diabetes | ECE2010

Diabetic mastopathy: imaging and management

Milosevic Zorica , Karapandzic Vesna Plesinac , Markovic Biljana , Radojicic Zoran , Borojevic Nenad

Diabetic mastopathy or sclerosing lymphocitic lobulitis of the breast in the patients with diabetes mellitus is a rare, benign disease that simulates breast malignancy.Among 489 women with firm breast lump(s), three women aged 32, 38 and 47 years with long standing diabetes mellitus type 1 were identified. Mammography and breast ultrasound were performed in all patients and contrast enhanced breast MRI in one patient with bilateral lumps. The histologica...

ea0022p364 | Diabetes | ECE2010

Evaluating the effects of vitamin D metabolism on glycemic parameters and atherosclerosis markers in prediabetic patients

Parildar Hulya , Takir Mumtaz , Unal Asli Dogruk , Cigerli Ozlem , Gulmez Oyku , Dinc Feyza , Guvener Nilgun Demirag

Background and aims: Hypovitaminosis D may be associated with metabolic parameters in addition to its well-known calcemic actions. There is increasing evidence that vitamin D metabolism affects the risk of insulin resistance although the underlying molecular mechanism of this association is not clear but on the basis of evidence it is not clear whether or not vitamin D supplementation therapy in vitamin D-deficient prediabetics affects the prevention of type 2 diabetes. Our st...

ea0022p365 | Diabetes | ECE2010

Analysis of incidence and risks factors of gestational diabetes in our area: comparison between two periods

Gil Beatriz Mantinan , Cano Reyes Luna , Sobrino Paula Sanchez , Carballeira Regina Palmeiro , Fernandez Concepcion Paramo , Alvarez Manuel Penin , Mayor Ricardo Victor Garcia

Introduction: Gestational diabetes (GD) is frequently associated with obesity and family history of diabetes. Besides these, other risks factors have been proposed, as previous GD, macrosomy or abnormal reproductive history.Methods: We conducted a retrospective comparative study including all patients diagnosed of GD during 1999 and 2008. The analyzed variables were incidence and risk factors of GD.Results: We included 384 patients...

ea0022p366 | Diabetes | ECE2010

Disease-modifying drugs in the treatment of intermittent claudication in patients with diabetes mellitus: the pilot study

Kuzina Irina , Gurieva Irina , Smirnov Sergey , Begma Inna , Kalinchenko Svetlana

Neuropathy and ischemia play paramount roles in the pathogenesis of diabetic vascular complications. Reduced tissue blood flow in experimental diabetic complications can be corrected by several disease-modifying drugs. Actovegin (deproteinised hemoderivative product) is considered to be a potential agent to stimulate cellular energy metabolism and improve cell function. Testosterone vascular effects can be mediated via increasing cardiac and muscle activity and endothelium dep...

ea0022p367 | Diabetes | ECE2010

Evaluation of HbA1c levels for the detection of prediabetes

Unal Asli Dogruk , Parildar Hulya , Takir Mumtaz , Cigerli Ozlem , Dinc Feyza , Guvener Nilgun Demirag

Objective: Recent recommendations suggest that HbA1c as a stable and standardised index of chronic glucose exposure over time and could be used as a better biochemical marker for the diagnosis of diabetes and prediabetes. Our aim in this cross-sectional study is to assess the validity of HbA1c as a screening tool for the detection of prediabetes in our patient population.Methods: The study was done in 265 patients selected among the participants attended...

ea0022p368 | Diabetes | ECE2010

Comparative evaluation of serum concentrations of a novel adipocytokine, fibroblast growth factor-21 in patients with type 2 diabetes and obesity

Matuszek Beata , Lenart-Lipinska Monika , Duma Dariusz , Solski Janusz , Nowakowski Andrzej

Fibroblast growth factor-21 (FGF-21) is a novel adipocytokine, which is believed to be involved in improvement of insulin sensitivity and pathogenesis of type 2 diabetes.The aim of the study was comparative assessment of FGF-21 concentrations in serum of patients with type 2 diabetes and evaluation of possible relationships between the studied cytokine and selected clinical and biochemical parameters.Forty-four patients with type 2...

ea0022p369 | Diabetes | ECE2010

The importance of the polymorphism of the AT1-receptor gene in diabetic nephropathy patients

Kolcsar Melinda , Endreffy Emoke

Objective of our study was to investigate the renoprotective efficacy of valsartan and enalapril depending on the genetic polymorphisms of the angiotensinic system.Material and methods: In 32 type 2 diabetic patients with microalbuminuria, DNA extraction and genotypization were performed. The studied polymorphism was A1166C of the AT1-receptor gene. Two types of antiangiotensinic drugs were used in order to reduce microalbuminuria: enalapril (20 mg/d) an...