Searchable abstracts of presentations at key conferences in endocrinology
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13th European Congress of Endocrinology

Poster Presentations

Steroid metabolism

ea0026p349 | Steroid metabolism | ECE2011

Beneficial effects of testosterone supplementation on prostatitis-like alterations in an animal model of metabolic syndrome

Morelli A , Filippi S , Vignozzi L , Comeglio P , Sarchielli E , Maneschi E , Cellai I , Vannelli G B , Maggi M

Introduction: Several studies suggested a direct relationship between metabolic syndrome (MetS) and increased risk of benign prostatic hyperplasia (BPH). An inflammatory component, mainly mediated by IL-8, has been proposed as the primary link between MetS and BPH pathogenesis. MetS, is often associated with testosterone (T) deficiency and an imbalance between the androgen and estrogen ratio characterizes BPH patients.Methods: We investigated the effects...

ea0026p350 | Steroid metabolism | ECE2011

Testosterone supplementation improves adipose tissue function in an animal model of metabolic syndrome

Morelli A , Maneschi E , Vignozzi L , Filippi S , Marchetta M , Mazzanti B , Calcagno A , Comeglio P , Cellai I , Vannelli G B , Maggi M

Introduction: Adipose tissue dysfunction is associated to metabolic syndrome (MetS), a clustering of cardio-metaboilic risk factors, including hypogonadism. We recently demonstrated that T supplementation was able to ameliorate the metabolic profile and reduce visceral fat accumulation in high-fat diet (HFD)-induced rabbit model of MetS.Methods: We evaluated the differentiation capacity of preadipocytes (rPAD) obtained from visceral fat of the following ...

ea0026p351 | Steroid metabolism | ECE2011

Evaluation of a method to measure long term cortisol levels in health and disease

Manenschijn L , Koper J W , van den Akker E L T , Feelders R A , Lamberts S W J , van Rossum E F C

Introduction: Elevated levels of cortisol are known to induce a wide range of pathology, e.g. abdominal obesity, type 2 diabetes and cardiovascular disease. Measuring serum and saliva cortisol is limited to one time point. Measurement of cortisol in scalp hair is a recently developed method to measure long term cortisol levels and might reflect cortisol exposure in patients with hyper- or hypo-cortisolism and in healthy persons. Our aims were to explore this measurement of cor...

ea0026p352 | Steroid metabolism | ECE2011

Effect of smoking cessation on steroid balance in postmenopausal women

Hruskovicova Hana , Duskova Michaela , Simunkova Katerina , Hill Martin , Pospisilova Hana , Kralikova Eva , Vondra Karel , Starka Luboslav

Introduction: Cigarette smoking is one of the most serious substance abuse problems. It is generally accepted that nicotine and other chemicals in tobacco smoke alter endocrine function in women, induce changes in female hormonal balance and likely contributes to reported associations of smoking with adverse reproductive outcomes, including menstrual dysfunction, infertility, and earlier menopause. Studies on the effects of smoking and smoking cessation on female sex hormones ...

ea0026p353 | Steroid metabolism | ECE2011

Activin A regulates local testosterone production and growth in prostate cancer through 17β-hydroxysteroid dehydrogenase

Hofland J , van Weerden W M , Steenbergen J , Dits N F J , Jenster G , de Jong F H

Introduction: Local androgen synthesis in prostate cancer (PC) could contribute to the development of castration-resistant PC (CRPC). Pathways controlling intratumoral steroidogenic enzyme expression in PC are unknown. Since activin has been implicated in regulation of PC growth and steroidogenic enzyme expression in other steroidogenic tissues, we investigated the effect of activin on intratumoral steroidogenesis in PC.Methods: Activin A effects and reg...

ea0026p354 | Steroid metabolism | ECE2011

Standardized high throughput LC–MS/MS steroid-quantification for clinical metabolomics

Koal Therese , Schmiederer Diane , Tuan Hai Pham , Rohring Cornelia , Rauh Manfred

Mass spectrometry based clinical metabolomics needs standardization of analytical assays to improve future inter-laboratory comparability and to become a successful new and established technology in clinical routine laboratories such as mass spectrometry based therapeutic drug monitoring (TDM) over the past 10 years. Steroid hormones are one of the most interesting endogenous metabolite class in clinical metabolomics today. Standardized determination of concentrations of stero...

ea0026p355 | Steroid metabolism | ECE2011

Salivary cortisol and testosterone: a comparison of salivary sample collection methods in healthy controls

Van Caenegem E , Wierckx K , Fiers T , Segers H , Vandersypt E , Kaufman J M , T'Sjoen G

Context: In population and psychosocial studies, saliva becomes increasingly popular, mainly due to its non-invasive collection methods and availability of free hormone fractions. However, few studies have evaluated the impact of collection devices on steroid hormone analysis; and none with the currently used methods.Objective: The aim of this pilot study was to minimize pre-analytical errors based on sample collection methods.Meth...

ea0026p356 | Steroid metabolism | ECE2011

Hormonal parameters and sex hormone receptor gene polymorphisms in males with autoimmune diseases

Doukas Charalampos , Saltiki Katerina , Terzidis Kostas , Mantzou Aimily , Sarika Lida , Cimponeriu Andriana , Sfikakis Petros , Alevizaki Maria

Introduction: Autoimmune disease (AD) is more common in women than in men. Sex hormones may play a role. Sex hormone receptors (SHR) are expressed in cells of the autoimmune system. We investigated the possible role of hormonal parameters and of common gene polymorphisms of the estrogen receptor alpha (ESR1), beta (ESR2) and androgen receptor (AR) in the presence of AD in men.Methods: In total 227 men were studied; 125 had at least o...

ea0026p357 | Steroid metabolism | ECE2011

Inhibitory effect of glucocorticoids on GLUT4 expression in visceral adipose tissue may be determined by increased 11β hydroxysteroid dehydrogenase 1 (11β-HSD1) expression

Mori R C T , Campello R S , Machado U F

Local glucocorticoids action depends on 11β hydroxysteroid dehydrogenase (11β-HSD) enzymes. Adipose tissues mainly express the 11β-HSD1, which activates inactive cortisone. Altered 11β-HSD1 and GLUT4 expressions have been reported in obesity. GLUT4 participates in the genesis and maintenance of insulin resistance/obesity, and could also be involved in the insulin resistant state observed in hypercortisolism. The aim of this study was to investigate the 11&#...

ea0026p358 | Steroid metabolism | ECE2011

Chronic effect of 17β estradiol on GLUT4 expression in 3T3-L1 adipocytes

Campello R S , Poletto A C , Furuya D T , Mori R C T , Machado U F

Introduction: Insulin resistance results from a combination of genetic and environmental factors and it may be considered the primary cause of the development of metabolic disorders, such as type 2 diabetes mellitus. It is characterized by a reduced ability of insulin sensitivity tissues to respond to normal levels of the hormone. In these tissues, glucose transport stimulated by insulin occurs through the glucose transporter 4 and alterations in its expression are related to ...